Standard name
Human Ortholog
Description Steryl-beta-glucosidase with broad specificity for aglycones; has a role in ergosteryl-beta-glucoside catabolism; required for normal vacuolar morphology; has similarity to the C. neoformans ergosteryl-beta-glucosidase EGCrP2; localizes to the cytosol
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 1.0 0.99 0.97 0.99 0.95 0.91 0.9 0.8 0.75 0.76 0.74 0.98 0.99 0.99 0.96 0.97 0.95 0.92 0.93 0.83 0.78 0.71 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.15 0.08 0.27 0.32 0.31 0.29 0 0 0 0 0 0 0 0 0 0.07 0.08 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.08 0 0 0.06 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 0 1 1 4 6
Bud 0 0 4 0 1 1 4 1 5 5 7 6 2 1 0 0 0 0 0 1 3 1 9 6
Bud Neck 0 0 0 0 2 1 0 2 1 0 1 0 0 0 0 0 0 0 0 0 1 0 3 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 1 0 1 4 1 1 3 0 1 1 5 1 0 4 5 4 2 0 1 1 1 1 2
Cytoplasm 290 249 233 180 279 256 266 327 172 183 196 169 308 285 352 192 206 146 277 246 204 130 172 219
Endoplasmic Reticulum 3 0 1 0 1 1 0 0 1 2 2 0 1 0 1 5 11 3 1 0 2 3 3 4
Endosome 1 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 2 3 0 0 1 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 2 0 0 0 3 0
Mitochondria 7 2 0 7 0 20 44 28 59 78 80 66 4 1 2 4 5 5 3 7 8 11 20 16
Nucleus 2 0 1 3 3 2 4 9 2 3 3 12 1 2 0 1 2 1 0 2 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0 0 0 3 1 1 0 1 3
Vac/Vac Membrane 0 0 0 2 1 5 1 12 4 10 6 11 0 1 1 5 8 13 0 1 14 10 13 12
Unique Cell Count 301 249 236 186 283 269 292 362 216 243 258 228 313 288 355 201 213 154 301 264 247 166 243 285
Labelled Cell Count 307 252 241 194 292 288 322 382 247 282 297 270 321 290 360 214 238 173 301 264 247 166 243 285


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.4 4.9 4.3 5.5 4.6 4.9 5.1 4.4 4.3 4.4 4.7 4.5 4.9 4.9 7.3 7.5 6.9 4.0 4.6 4.8
Std Deviation (1e-4) 0.5 0.6 1.2 1.1 1.4 1.1 1.4 0.9 1.0 0.9 1.2 1.0 1.0 1.4 1.1 1.4 1.4 1.3 1.1 2.1 1.8
Intensity Change (Log2) – – – -0.17 0.18 -0.06 0.02 0.06 -0.13 -0.18 -0.14 -0.05 -0.1 0.01 0.01 0.6 0.62 0.5 -0.28 -0.07 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3807 -0.4922 -0.4816 -0.2568 0.5168 -0.3412 0.2391 -0.1476 -0.3037 -0.3428 -1.1059 -0.6163 -1.0688 -0.8677 -0.9024 -0.5351 -1.4596 -0.9304
Actin 0.0796 0.0024 0.0175 0.0425 0.0026 0.0068 0.0635 0.0003 0.0149 0.0005 0.0001 0.0002 0.031 0.0001 0.0108 0.0041 0 0.0025
Bud 0.0017 0.0029 0.0013 0.0215 0.0007 0.0001 0.0056 0.003 0.0005 0 0 0.0001 0.0005 0.0062 0.0015 0.0037 0 0.0003
Bud Neck 0.0103 0.0001 0.0007 0.0018 0.0006 0.0004 0.0025 0.0001 0.0006 0.0002 0.0001 0.0002 0.0013 0.0001 0.0003 0.0005 0 0.0001
Bud Periphery 0.0038 0.0031 0.0066 0.0169 0.0013 0.0002 0.0102 0.0079 0.0007 0.0001 0 0.0002 0.0007 0.0023 0.0018 0.0066 0 0.0005
Bud Site 0.0073 0.0075 0.0024 0.0307 0.0013 0.0001 0.0051 0.0004 0.0036 0.0003 0 0 0.0066 0.0066 0.0039 0.0264 0 0.0002
Cell Periphery 0.0009 0.0004 0.0003 0.001 0.0003 0.0001 0.0006 0.0002 0.0002 0 0 0 0.0004 0.0002 0.0001 0.0004 0 0
Cytoplasm 0.0612 0.1198 0.0767 0.1256 0.0998 0.1677 0.1 0.1613 0.1965 0.0981 0.2685 0.2483 0.0826 0.1379 0.1361 0.1263 0.0665 0.1695
Cytoplasmic Foci 0.0701 0.0068 0.0228 0.0171 0.0085 0.0088 0.0287 0.0043 0.0153 0.0408 0.0056 0.0034 0.0361 0.0019 0.0097 0.0122 0.0006 0.0027
Eisosomes 0.0012 0.0002 0.0003 0.0001 0.0001 0.0002 0.0004 0.0001 0.0001 0 0 0.0001 0.0004 0.0001 0.0003 0.0001 0 0.0001
Endoplasmic Reticulum 0.0104 0.0015 0.001 0.001 0.0038 0.0015 0.0057 0.0014 0.0016 0.0004 0.0021 0.0009 0.002 0.0005 0.002 0.0033 0.0002 0.0009
Endosome 0.0534 0.0015 0.0074 0.0167 0.0381 0.0017 0.0283 0.0027 0.0049 0.0023 0.0041 0.0005 0.0113 0.0004 0.0081 0.0091 0 0.0099
Golgi 0.0237 0.002 0.006 0.0081 0.0043 0.0028 0.0109 0.0001 0.0027 0.0024 0.0001 0 0.0059 0 0.0037 0.0019 0 0.0048
Lipid Particles 0.0296 0.0007 0.0057 0.0283 0.0433 0.0039 0.012 0.0003 0.0111 0.0054 0.0001 0.0003 0.0103 0.0001 0.0074 0.0035 0 0.0009
Mitochondria 0.0146 0.0015 0.0115 0.0036 0.0388 0.0037 0.0073 0.0003 0.0094 0.0003 0.0001 0.0002 0.0041 0.0001 0.0035 0.0022 0 0.004
None 0.544 0.8443 0.8167 0.602 0.6435 0.7792 0.6704 0.8127 0.7235 0.8343 0.7173 0.7438 0.7835 0.842 0.7927 0.7516 0.9323 0.7887
Nuclear Periphery 0.0194 0.0008 0.0026 0.001 0.0452 0.0009 0.009 0.0005 0.0016 0.0002 0.0003 0.0003 0.0008 0.0002 0.0023 0.0032 0.0001 0.0028
Nucleolus 0.0019 0.0002 0.0004 0.0116 0.0028 0.0002 0.0011 0.0002 0.0004 0.0001 0 0.0001 0.0004 0.0001 0.0005 0.0016 0 0.0001
Nucleus 0.0123 0.0008 0.0081 0.0266 0.0424 0.0015 0.0081 0.0008 0.0014 0.0003 0.0005 0.0006 0.0008 0.0007 0.0025 0.0025 0.0001 0.0099
Peroxisomes 0.0328 0.0023 0.0047 0.0252 0.0029 0.0184 0.0041 0.0001 0.008 0.0126 0 0.0001 0.0141 0 0.0078 0.0021 0 0.0003
Punctate Nuclear 0.0125 0.0006 0.0057 0.0028 0.0034 0.0015 0.0203 0.0019 0.0015 0.0012 0.0002 0.0005 0.0061 0.0002 0.0034 0.0334 0.0001 0.0005
Vacuole 0.0062 0.0006 0.001 0.0144 0.0095 0.0003 0.0043 0.0012 0.0012 0.0003 0.0007 0.0002 0.0011 0.0002 0.0011 0.0043 0 0.0008
Vacuole Periphery 0.0031 0.0001 0.0006 0.0013 0.0069 0.0001 0.002 0.0002 0.0004 0.0001 0.0001 0 0.0002 0 0.0005 0.0009 0 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2118 5.5873 6.0596 9.7619 7.792 11.5325 12.2764 12.172 12.937 10.1988
Translational Efficiency 0.7428 1.0618 0.8737 0.69 0.798 0.7021 0.6594 0.6894 0.7102 0.7073

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1895 158 256 673 2148 1534 877 544 4043 1692 1133 1217

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 641.12 808.74 1042.38 856.90 682.63 655.10 849.30 1021.44 663.17 669.45 892.93 930.45
Standard Deviation 74.15 112.11 113.61 103.16 88.22 87.00 107.58 151.78 84.50 100.17 135.63 151.25
Intensity Change Log 2 — 0.335082 0.701215 0.418532 — -0.059389 0.315170 0.581429 — 0.145127 0.515037 0.504828

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000061 0.000691 0.001395 0.000883 0.000075 0.000355 0.000233 0.000173 0.000068 0.000386 0.000496 0.000565
Bud Neck 0.011893 0.030771 0.005577 0.006273 0.013882 0.037276 0.002933 0.010431 0.012950 0.036669 0.003530 0.008132
Bud Site 0.003990 0.014267 0.010385 0.035082 0.002983 0.014948 0.010367 0.033170 0.003455 0.014884 0.010371 0.034227
Cell Periphery 0.000095 0.000296 0.000342 0.000173 0.000093 0.000138 0.000193 0.000105 0.000094 0.000153 0.000227 0.000142
Cytoplasm 0.558014* 0.319151* 0.183750* 0.452994* 0.521178* 0.397558* 0.387383* 0.646677* 0.538444* 0.390237* 0.341372* 0.539570*
Cytoplasmic Foci 0.145771 0.314191* 0.008801 0.016776 0.188411* 0.275196* 0.013413 0.001922 0.168425 0.278837* 0.012371 0.010136
Eisosomes 0.000103 0.000254 0.000113 0.000060 0.000113 0.000177 0.000051 0.000010 0.000108 0.000184 0.000065 0.000038
Endoplasmic Reticulum 0.000577 0.001098 0.017826 0.002804 0.000763 0.000668 0.002872 0.000231 0.000676 0.000708 0.006250 0.001654
Endosome 0.006771 0.037076 0.005704 0.006910 0.007727 0.032258 0.002362 0.000312 0.007279 0.032708 0.003117 0.003961
Golgi 0.000786 0.004800 0.000188 0.002284 0.001795 0.006345 0.000083 0.000092 0.001322 0.006201 0.000107 0.001304
Lipid Particles 0.005526 0.007447 0.003867 0.001249 0.006550 0.010894 0.001671 0.000123 0.006070 0.010572 0.002167 0.000746
Mitochondria 0.004066 0.006188 0.001123 0.006642 0.003397 0.017097 0.000617 0.002205 0.003710 0.016078 0.000732 0.004659
Mitotic Spindle 0.000571 0.001274 0.001220 0.021262 0.001314 0.003851 0.008262 0.012034 0.000965 0.003610 0.006671 0.017137
None 0.006617 0.001245 0.002948 0.004864 0.003899 0.003441 0.008621 0.008905 0.005173 0.003236 0.007339 0.006671
Nuclear Periphery 0.000360 0.000520 0.002690 0.001520 0.000471 0.000531 0.001462 0.000535 0.000419 0.000530 0.001740 0.001080
Nuclear Periphery Foci 0.000345 0.001083 0.005543 0.001286 0.000351 0.000510 0.001237 0.000063 0.000348 0.000563 0.002210 0.000739
Nucleolus 0.001071 0.001929 0.000634 0.000287 0.001330 0.003500 0.000523 0.000341 0.001209 0.003353 0.000548 0.000311
Nucleus 0.198896* 0.111049 0.585884* 0.279278* 0.197058* 0.099774 0.493710* 0.261767* 0.197920* 0.100827 0.514537* 0.271451*
Peroxisomes 0.002041 0.047510 0.000463 0.002720 0.003358 0.018890 0.000334 0.000180 0.002741 0.021562 0.000363 0.001585
Vacuole 0.051827 0.097721 0.161207 0.153964 0.044465 0.073223 0.063445 0.020133 0.047915 0.075511 0.085534 0.094141
Vacuole Periphery 0.000617 0.001438 0.000339 0.002690 0.000790 0.003370 0.000228 0.000592 0.000709 0.003190 0.000253 0.001752

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.38 -13.35 -4.29 0.73 6.92 -6.45 -9.04 -1.05 3.69 3.61 -7.61 -14.53 -4.37 0.44 3.58
Bud Neck -3.09 3.32 5.11 4.22 1.00 -9.44 10.16 2.41 10.70 -6.02 -10.60 11.23 5.47 13.15 -5.14
Bud Site -3.22 -2.22 -4.03 -0.68 -2.01 -8.87 -2.16 -4.46 -1.14 -3.14 -8.96 -2.62 -6.04 -1.17 -3.87
Cell Periphery -2.98 -11.39 -3.00 2.50 9.40 -5.69 -3.41 2.74 6.57 4.33 -6.28 -6.68 -0.96 4.82 5.95
Cytoplasm 10.98 34.00 21.88 0.41 -11.16 13.53 20.58 -0.10 -9.20 -14.83 18.59 31.55 14.92 -1.33 -12.02
Cytoplasmic Foci -9.00 36.12 34.62 16.75 -3.93 -12.10 42.32 45.46 47.04 9.09 -17.64 54.50 56.05 48.78 2.30
Eisosomes -3.09 -2.50 4.10 3.69 6.24 -5.05 8.69 23.12 13.80 15.14 -6.19 5.91 14.93 11.56 8.56
Endoplasmic Reticulum -2.57 -16.01 -15.57 -9.39 12.78 0.78 -14.09 3.25 2.55 16.26 -0.37 -18.93 -12.39 -11.41 13.51
Endosome -4.35 1.01 4.78 4.91 4.16 -12.19 9.98 14.85 16.53 12.43 -13.28 10.08 11.29 16.28 3.95
Golgi -3.56 7.66 0.62 3.56 -0.86 -5.77 5.12 5.14 8.90 0.51 -7.19 6.68 3.53 8.56 -1.05
Lipid Particles -0.98 2.61 8.33 3.08 7.51 -4.41 11.98 16.88 11.98 11.55 -5.08 11.78 17.06 11.54 9.38
Mitochondria -0.80 3.14 1.70 1.81 -1.24 -7.53 5.04 2.92 8.89 -2.61 -7.37 5.52 2.78 8.73 -2.28
Mitotic Spindle -1.21 -2.06 -5.08 -4.71 -4.31 -2.31 -2.22 -3.02 -1.88 -1.10 -2.89 -2.64 -5.82 -3.99 -3.03
None 6.10 4.84 1.05 -6.28 -4.62 0.59 -2.09 -7.16 -8.70 -3.89 2.98 -0.19 -3.86 -7.52 -2.94
Nuclear Periphery -1.38 -18.64 -16.30 -11.11 1.88 -0.49 -16.83 -5.85 -4.85 3.29 -1.09 -23.66 -15.63 -11.61 0.93
Nuclear Periphery Foci -2.74 -9.32 -9.10 -1.95 6.81 -1.72 -8.87 4.04 4.59 10.54 -2.63 -12.39 -7.46 -4.25 8.05
Nucleolus -1.51 5.51 6.68 2.89 2.07 -3.33 5.35 4.76 4.80 -1.07 -3.68 7.44 7.47 5.14 0.15
Nucleus 6.50 -19.27 -11.83 -13.47 10.95 18.43 -30.86 -15.12 -23.70 10.08 21.52 -36.56 -18.85 -30.19 15.93
Peroxisomes -4.74 8.61 2.86 4.89 -0.57 -12.06 9.76 10.23 15.12 2.23 -12.95 12.79 7.44 14.53 -0.55
Vacuole -5.00 -12.62 -21.45 -12.21 -3.16 -9.51 -12.28 -1.51 4.37 9.52 -9.77 -16.41 -18.53 -13.91 -3.56
Vacuole Periphery -2.35 4.96 -0.18 1.59 -1.14 -6.72 7.51 2.63 7.45 -0.86 -7.17 9.37 1.02 6.86 -1.63
Standard name
Human Ortholog
Description Steryl-beta-glucosidase with broad specificity for aglycones; has a role in ergosteryl-beta-glucoside catabolism; required for normal vacuolar morphology; has similarity to the C. neoformans ergosteryl-beta-glucosidase EGCrP2; localizes to the cytosol
Localization
Cell Percentages cytoplasm (39%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Egh1

Egh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Egh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available