Standard name | |
---|---|
Human Ortholog | |
Description | Protein required for localizing proteasomes to the nucleus; involved in cotranslational protein degradation; mediates interaction between nuclear import factor Srp1p and the proteasome; Sts1p and Srp1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation; involved in ubiquitin-mediated protein degradation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Bud | 2 | 1 | 0 | 0 | 0 | 0 | 7 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 1 | 3 | 6 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 60 | 33 | 11 | 49 | 135 | 111 | 144 | 65 | 66 | 102 | 34 | 12 | 73 | 195 | 206 | 84 | 96 | 89 | 57 | 24 | 10 | 44 | 117 | 159 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 5 | 7 | 10 | 2 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 1 | 0 | 9 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 0 | 21 | 16 | 26 | 14 | 6 | 2 | 3 | 3 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 4 | 2 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 131 | 7 | 0 | 4 | 0 | 12 | 116 | 51 | 116 | 114 | 88 | 30 | 0 | 0 | 0 | 6 | 2 | 15 | 23 | 4 | 8 | 7 | 11 | 8 |
Nucleus | 96 | 24 | 4 | 20 | 3 | 4 | 22 | 7 | 25 | 19 | 20 | 8 | 35 | 0 | 1 | 22 | 13 | 29 | 161 | 66 | 14 | 47 | 5 | 7 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 12 | 1 | 1 | 0 | 0 | 0 |
Nucleolus | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 2 | 1 | 1 | 2 | 2 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 22 | 5 | 4 | 11 | 8 | 17 |
Vac/Vac Membrane | 101 | 90 | 16 | 33 | 21 | 16 | 28 | 7 | 14 | 14 | 7 | 5 | 52 | 39 | 10 | 128 | 137 | 143 | 114 | 39 | 16 | 48 | 15 | 16 |
Unique Cell Count | 326 | 137 | 27 | 109 | 156 | 130 | 259 | 112 | 180 | 218 | 116 | 42 | 143 | 226 | 215 | 227 | 234 | 275 | 437 | 162 | 66 | 178 | 183 | 234 |
Labelled Cell Count | 394 | 159 | 31 | 117 | 163 | 148 | 321 | 132 | 225 | 256 | 149 | 56 | 164 | 238 | 218 | 271 | 281 | 322 | 437 | 162 | 66 | 178 | 183 | 234 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 5.0 | 5.6 | 5.2 | 5.1 | 4.0 | 3.7 | 4.1 | 3.4 | 3.9 | 3.6 | 3.7 | 6.1 | 5.8 | 5.5 | 7.4 | 6.9 | 6.8 | 5.7 | 6.0 | 6.3 |
Std Deviation (1e-4) | 0.5 | 0.7 | 0.8 | 1.5 | 1.4 | 1.5 | 1.2 | 1.0 | 1.2 | 1.6 | 1.8 | 1.3 | 1.1 | 1.3 | 1.0 | 1.8 | 1.9 | 1.9 | 1.7 | 2.3 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.14 | -0.47 | -0.6 | -0.45 | -0.7 | -0.52 | -0.63 | -0.58 | 0.12 | 0.05 | -0.02 | 0.41 | 0.31 | 0.28 | 0.03 | 0.11 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.4 | 5.5 | 5.1 | 1.5 | 1.6 | -0.4 | 0.6 | -1.2 | -1.0 | 1.0 | 5.8 | 8.9 | -0.4 | 0 | -0.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.8 | -5.5 | -5.5 | 0 | 0 | 0 | 0 | 0 | -4.2 | -2.2 | -5.0 | 0 | -0.3 | -0.1 | -0.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0379 | 0.1043 | 0.2276 | -0.1079 | -0.2279 | 0.1474 | 0.2235 | 0.6301 | 0.5586 | 0.944 | 0.4485 | 0.3915 | 0.1808 | 0.0448 | -0.1631 | 0.0695 | 0.5108 | -0.037 |
Actin | 0.0066 | 0.0008 | 0.0047 | 0.003 | 0.0118 | 0.0007 | 0.0304 | 0.0005 | 0.0216 | 0.0035 | 0.0058 | 0.0005 | 0.0427 | 0.0017 | 0.0005 | 0.0108 | 0.0048 | 0.0003 |
Bud | 0.0001 | 0.0018 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0006 | 0.0002 | 0.0017 | 0.0001 | 0.0007 | 0.0126 | 0.0009 | 0.0033 | 0.0001 | 0.0029 | 0.0015 | 0.0001 |
Bud Neck | 0.0016 | 0.0008 | 0.0098 | 0.0014 | 0.0014 | 0.0017 | 0.0061 | 0.0007 | 0.0044 | 0.0012 | 0.0033 | 0.0023 | 0.0043 | 0.0007 | 0.0006 | 0.0309 | 0.0013 | 0.0022 |
Bud Periphery | 0.0001 | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0 | 0.0007 | 0.0002 | 0.001 | 0.0001 | 0.0007 | 0.0029 | 0.0011 | 0.0004 | 0.0001 | 0.0031 | 0.0028 | 0.0001 |
Bud Site | 0.0008 | 0.0051 | 0.0004 | 0.0006 | 0.0008 | 0.0001 | 0.0027 | 0.0027 | 0.0104 | 0.0003 | 0.0009 | 0.0006 | 0.0147 | 0.005 | 0.0007 | 0.0282 | 0.0053 | 0.0002 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0014 | 0.0003 | 0 |
Cytoplasm | 0.2115 | 0.2402 | 0.1457 | 0.1835 | 0.1342 | 0.1188 | 0.1733 | 0.2594 | 0.2204 | 0.1813 | 0.164 | 0.141 | 0.3363 | 0.3066 | 0.2787 | 0.341 | 0.4569 | 0.2676 |
Cytoplasmic Foci | 0.0053 | 0.0054 | 0.0058 | 0.0062 | 0.0129 | 0.0064 | 0.011 | 0.0038 | 0.0134 | 0.015 | 0.0217 | 0.007 | 0.0142 | 0.0164 | 0.0078 | 0.0075 | 0.0113 | 0.0059 |
Eisosomes | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0139 | 0.0063 | 0.0045 | 0.0063 | 0.0031 | 0.0027 | 0.0194 | 0.0064 | 0.005 | 0.0061 | 0.0092 | 0.0028 | 0.0129 | 0.0133 | 0.0129 | 0.0082 | 0.013 | 0.0052 |
Endosome | 0.0134 | 0.0075 | 0.0047 | 0.0122 | 0.0165 | 0.0053 | 0.0251 | 0.0063 | 0.0068 | 0.0037 | 0.0122 | 0.0056 | 0.0173 | 0.0077 | 0.0075 | 0.0059 | 0.0308 | 0.0084 |
Golgi | 0.0011 | 0.0002 | 0.0004 | 0.0006 | 0.0058 | 0.0001 | 0.0033 | 0.0005 | 0.0037 | 0.0014 | 0.0044 | 0.0003 | 0.0069 | 0.0008 | 0.0002 | 0.0002 | 0.0128 | 0.0001 |
Lipid Particles | 0.0019 | 0.0005 | 0.0015 | 0.0008 | 0.0136 | 0.0007 | 0.0037 | 0.0006 | 0.0028 | 0.0062 | 0.0166 | 0.0009 | 0.0095 | 0.0006 | 0.0005 | 0.0002 | 0.0236 | 0.0003 |
Mitochondria | 0.0014 | 0.0012 | 0.0013 | 0.0023 | 0.0014 | 0.0006 | 0.0017 | 0.0008 | 0.0038 | 0.0011 | 0.0037 | 0.0008 | 0.009 | 0.0024 | 0.0006 | 0.0008 | 0.0385 | 0.0006 |
None | 0.3361 | 0.2491 | 0.3944 | 0.1776 | 0.5428 | 0.2209 | 0.1307 | 0.1829 | 0.1616 | 0.1566 | 0.1334 | 0.1488 | 0.0663 | 0.2048 | 0.0964 | 0.1176 | 0.0509 | 0.1399 |
Nuclear Periphery | 0.0505 | 0.0313 | 0.033 | 0.0509 | 0.017 | 0.0164 | 0.0418 | 0.0261 | 0.0318 | 0.0251 | 0.0381 | 0.0139 | 0.0419 | 0.0465 | 0.0274 | 0.0276 | 0.0512 | 0.0219 |
Nucleolus | 0.0038 | 0.0049 | 0.0029 | 0.0033 | 0.006 | 0.0062 | 0.0037 | 0.003 | 0.0034 | 0.0021 | 0.0053 | 0.0056 | 0.0017 | 0.0042 | 0.0021 | 0.0012 | 0.0022 | 0.0023 |
Nucleus | 0.312 | 0.3921 | 0.3251 | 0.4832 | 0.1698 | 0.5423 | 0.4997 | 0.4798 | 0.4534 | 0.5318 | 0.4336 | 0.5902 | 0.3734 | 0.3424 | 0.5397 | 0.3974 | 0.26 | 0.5226 |
Peroxisomes | 0.0004 | 0.0003 | 0.001 | 0.0005 | 0.0033 | 0.0011 | 0.0008 | 0.0003 | 0.0039 | 0.0184 | 0.0581 | 0.0005 | 0.0148 | 0.0008 | 0.0002 | 0.0001 | 0.0064 | 0.0001 |
Punctate Nuclear | 0.0336 | 0.046 | 0.0619 | 0.0633 | 0.0571 | 0.0737 | 0.0334 | 0.0211 | 0.0485 | 0.044 | 0.0838 | 0.0595 | 0.025 | 0.0374 | 0.0213 | 0.0125 | 0.0142 | 0.0186 |
Vacuole | 0.0045 | 0.0049 | 0.0015 | 0.0022 | 0.0013 | 0.0017 | 0.0092 | 0.0039 | 0.0017 | 0.0013 | 0.0032 | 0.0036 | 0.005 | 0.0039 | 0.0023 | 0.0019 | 0.0082 | 0.0029 |
Vacuole Periphery | 0.001 | 0.0008 | 0.0006 | 0.0014 | 0.0005 | 0.0004 | 0.002 | 0.0005 | 0.0005 | 0.0005 | 0.0012 | 0.0005 | 0.0014 | 0.0009 | 0.0005 | 0.0005 | 0.004 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.0332 | 20.5495 | 22.1105 | 28.0355 | 18.6278 | 13.7849 | 24.4137 | 31.9034 | 24.1564 | 30.6756 |
Translational Efficiency | 1.0471 | 1.4648 | 1.3173 | 0.9975 | 1.8804 | 2.9053 | 1.4363 | 1.0527 | 1.4902 | 1.3753 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1384 | 1080 | 180 | 1594 | 1505 | 2358 | 211 | 1096 | 2889 | 3438 | 391 | 2690 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 722.00 | 700.15 | 961.92 | 988.23 | 670.21 | 758.96 | 988.12 | 994.31 | 695.02 | 740.49 | 976.06 | 990.71 |
Standard Deviation | 84.81 | 124.29 | 101.42 | 138.01 | 78.15 | 94.42 | 109.02 | 133.73 | 85.42 | 108.22 | 106.39 | 136.31 |
Intensity Change Log 2 | — | -0.044335 | 0.413918 | 0.452848 | — | 0.179411 | 0.560073 | 0.569083 | — | 0.067712 | 0.486127 | 0.509973 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000061 | 0.000406 | 0.001167 | 0.001332 | 0.000053 | 0.000262 | 0.000848 | 0.000685 | 0.000057 | 0.000307 | 0.000995 | 0.001069 |
Bud Neck | 0.003625 | 0.010231 | 0.003482 | 0.013028 | 0.004006 | 0.014702 | 0.005367 | 0.010879 | 0.003823 | 0.013297 | 0.004499 | 0.012153 |
Bud Site | 0.000831 | 0.005590 | 0.002143 | 0.006807 | 0.002540 | 0.007954 | 0.015035 | 0.005824 | 0.001721 | 0.007212 | 0.009100 | 0.006406 |
Cell Periphery | 0.000126 | 0.000172 | 0.000249 | 0.000153 | 0.000135 | 0.000376 | 0.000208 | 0.000132 | 0.000130 | 0.000312 | 0.000227 | 0.000144 |
Cytoplasm | 0.275880* | 0.318102* | 0.086334 | 0.081029 | 0.318813* | 0.243030* | 0.122271* | 0.102103* | 0.298246* | 0.266613* | 0.105727* | 0.089616 |
Cytoplasmic Foci | 0.078579 | 0.061680 | 0.004581 | 0.002454 | 0.073067 | 0.051584 | 0.010915 | 0.004539 | 0.075708 | 0.054756 | 0.007999 | 0.003304 |
Eisosomes | 0.000066 | 0.000056 | 0.000098 | 0.000025 | 0.000045 | 0.000168 | 0.000090 | 0.000022 | 0.000055 | 0.000133 | 0.000094 | 0.000024 |
Endoplasmic Reticulum | 0.003112 | 0.005729 | 0.017456 | 0.012226 | 0.002296 | 0.003174 | 0.011553 | 0.010445 | 0.002687 | 0.003977 | 0.014271 | 0.011500 |
Endosome | 0.003810 | 0.006848 | 0.003908 | 0.003191 | 0.002472 | 0.005970 | 0.004281 | 0.002167 | 0.003113 | 0.006246 | 0.004109 | 0.002774 |
Golgi | 0.000405 | 0.001553 | 0.000156 | 0.001747 | 0.000701 | 0.001472 | 0.000105 | 0.000733 | 0.000559 | 0.001497 | 0.000129 | 0.001334 |
Lipid Particles | 0.019234 | 0.009452 | 0.003706 | 0.000617 | 0.012140 | 0.010584 | 0.002837 | 0.000990 | 0.015538 | 0.010229 | 0.003237 | 0.000769 |
Mitochondria | 0.003546 | 0.006277 | 0.000839 | 0.003841 | 0.005240 | 0.009373 | 0.000928 | 0.002023 | 0.004428 | 0.008401 | 0.000887 | 0.003100 |
Mitotic Spindle | 0.000487 | 0.011772 | 0.000821 | 0.028156 | 0.001903 | 0.009511 | 0.000689 | 0.022286 | 0.001225 | 0.010221 | 0.000750 | 0.025765 |
None | 0.010757 | 0.031329 | 0.003735 | 0.007325 | 0.008052 | 0.011692 | 0.011114 | 0.006324 | 0.009348 | 0.017860 | 0.007717 | 0.006917 |
Nuclear Periphery | 0.007487 | 0.005547 | 0.003749 | 0.009819 | 0.004264 | 0.008353 | 0.003270 | 0.006093 | 0.005808 | 0.007471 | 0.003490 | 0.008301 |
Nuclear Periphery Foci | 0.003152 | 0.005540 | 0.010930 | 0.002800 | 0.001412 | 0.001897 | 0.003171 | 0.004685 | 0.002246 | 0.003041 | 0.006743 | 0.003568 |
Nucleolus | 0.005480 | 0.013785 | 0.003924 | 0.001477 | 0.003155 | 0.009089 | 0.003254 | 0.002658 | 0.004269 | 0.010564 | 0.003562 | 0.001958 |
Nucleus | 0.569505* | 0.476041* | 0.802714* | 0.795291* | 0.549914* | 0.587360* | 0.739459* | 0.800205* | 0.559299* | 0.552391* | 0.768579* | 0.797293* |
Peroxisomes | 0.002911 | 0.005413 | 0.000510 | 0.000332 | 0.001938 | 0.004657 | 0.000468 | 0.001004 | 0.002404 | 0.004894 | 0.000487 | 0.000606 |
Vacuole | 0.010446 | 0.023298 | 0.049210 | 0.026322 | 0.007510 | 0.017631 | 0.063881 | 0.015743 | 0.008916 | 0.019411 | 0.057127 | 0.022012 |
Vacuole Periphery | 0.000501 | 0.001179 | 0.000286 | 0.002028 | 0.000344 | 0.001163 | 0.000256 | 0.000458 | 0.000419 | 0.001168 | 0.000270 | 0.001389 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.15 | -9.25 | -6.11 | -3.94 | 0.64 | -3.43 | -5.48 | -17.38 | -9.35 | 1.23 | -6.14 | -9.34 | -9.21 | -6.60 | 1.02 |
Bud Neck | -6.57 | -1.33 | -13.53 | -6.25 | -13.36 | -8.71 | -1.23 | -10.04 | -0.43 | -6.15 | -10.69 | -1.66 | -16.83 | -4.01 | -12.63 |
Bud Site | -6.56 | -5.06 | -6.95 | 0.15 | -3.74 | -3.62 | -1.86 | -2.33 | 1.41 | 1.15 | -5.84 | -2.29 | -5.35 | 1.51 | 0.64 |
Cell Periphery | -2.73 | -5.27 | -1.68 | 1.58 | 4.63 | -4.71 | -3.05 | -0.09 | 4.99 | 4.00 | -5.27 | -5.79 | -1.10 | 5.08 | 5.98 |
Cytoplasm | -3.30 | 18.52 | 30.16 | 28.32 | 1.58 | 9.49 | 17.69 | 32.55 | 27.08 | 2.11 | 5.52 | 25.08 | 44.99 | 40.56 | 2.85 |
Cytoplasmic Foci | 3.40 | 21.63 | 22.80 | 16.80 | 4.07 | 5.29 | 16.57 | 20.98 | 21.41 | 2.79 | 6.82 | 26.31 | 31.07 | 27.89 | 3.90 |
Eisosomes | 1.08 | -4.50 | 8.44 | 7.39 | 7.64 | -1.79 | -8.29 | 4.26 | 1.96 | 9.71 | -1.68 | -8.89 | 9.10 | 2.11 | 12.20 |
Endoplasmic Reticulum | -5.81 | -9.97 | -19.07 | -11.74 | 3.19 | -3.10 | -9.13 | -17.41 | -15.74 | 2.67 | -5.46 | -13.41 | -26.42 | -22.17 | 3.94 |
Endosome | -3.85 | -0.86 | 3.13 | 7.14 | 4.50 | -5.46 | -2.18 | 1.58 | 7.68 | 3.42 | -6.20 | -2.22 | 3.26 | 10.04 | 5.17 |
Golgi | -4.47 | 3.94 | -0.88 | 2.92 | -1.96 | -1.89 | 2.97 | 1.70 | 3.29 | -1.90 | -3.54 | 3.88 | 0.47 | 3.69 | -2.54 |
Lipid Particles | 4.77 | 8.67 | 10.69 | 8.24 | 6.29 | 1.08 | 7.58 | 9.58 | 12.01 | 6.24 | 4.30 | 11.48 | 14.30 | 14.67 | 9.01 |
Mitochondria | -3.18 | 4.70 | -0.38 | 3.72 | -13.01 | -2.99 | 3.57 | 2.33 | 8.66 | -8.54 | -4.53 | 5.16 | 1.72 | 8.69 | -14.85 |
Mitotic Spindle | -5.56 | -1.52 | -9.28 | -4.24 | -9.01 | -4.28 | 1.02 | -5.76 | -3.39 | -6.14 | -7.00 | 0.33 | -10.70 | -5.96 | -10.86 |
None | -6.25 | 5.75 | 3.64 | 8.44 | -3.36 | -3.12 | 0.52 | 2.93 | 6.19 | 0.88 | -6.20 | 3.60 | 4.51 | 10.11 | -0.56 |
Nuclear Periphery | 1.67 | 4.28 | -4.82 | -6.57 | -9.27 | -5.12 | -1.31 | -6.14 | 0.10 | -3.36 | -2.77 | 1.81 | -7.50 | -4.48 | -8.90 |
Nuclear Periphery Foci | -3.28 | -1.52 | 0.49 | 4.17 | 1.59 | -1.86 | -3.18 | -2.83 | -1.87 | 1.42 | -2.60 | -2.51 | -2.05 | 0.36 | 2.07 |
Nucleolus | -5.60 | 1.38 | 5.24 | 8.06 | 1.03 | -7.26 | 1.54 | 1.25 | 7.38 | -0.06 | -8.44 | 1.96 | 4.72 | 11.58 | 0.87 |
Nucleus | 7.44 | -11.34 | -21.67 | -27.14 | -0.62 | -3.68 | -7.41 | -24.67 | -22.81 | -4.87 | 0.96 | -12.62 | -32.77 | -33.95 | -4.11 |
Peroxisomes | -2.92 | 4.32 | 4.84 | 7.59 | 4.05 | -4.95 | 6.97 | 5.09 | 7.94 | -0.22 | -5.05 | 6.79 | 7.00 | 11.26 | 1.43 |
Vacuole | -6.65 | -7.52 | -13.47 | -5.96 | 3.38 | -8.71 | -8.55 | -10.28 | -6.33 | 6.12 | -10.43 | -11.14 | -17.15 | -10.23 | 6.79 |
Vacuole Periphery | -5.10 | 3.17 | -1.00 | 1.43 | -1.79 | -7.19 | 2.06 | 0.90 | 6.81 | -0.22 | -8.37 | 3.78 | -0.89 | 3.55 | -1.85 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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