Standard name
Human Ortholog
Description Protein required for localizing proteasomes to the nucleus; involved in cotranslational protein degradation; mediates interaction between nuclear import factor Srp1p and the proteasome; Sts1p and Srp1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation; involved in ubiquitin-mediated protein degradation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.24 0.41 0.45 0.87 0.85 0.56 0.58 0.37 0.47 0.29 0.29 0.51 0.86 0.96 0.37 0.41 0.32 0.13 0.15 0.17 0.25 0.64 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.09 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0.05 0 0 0 0.09 0.45 0.46 0.64 0.52 0.76 0.71 0 0 0 0 0 0.05 0.05 0 0.13 0 0.06 0
Nucleus 0.29 0.18 0.15 0.18 0 0 0.08 0.06 0.14 0.09 0.17 0.19 0.24 0 0 0.1 0.06 0.11 0.37 0.41 0.22 0.26 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0.06 0.05 0.07
Vac/Vac Membrane 0.31 0.66 0.59 0.3 0.13 0.12 0.11 0.06 0.08 0.06 0.06 0.12 0.36 0.17 0.05 0.56 0.59 0.52 0.26 0.25 0.26 0.27 0.09 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Bud 2 1 0 0 0 0 7 0 3 0 0 0 0 0 0 0 0 0 4 4 1 3 6 6
Bud Neck 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 2 2 0 1 3 4
Bud Site 0 0 0 0 0 0 1 0 0 2 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 60 33 11 49 135 111 144 65 66 102 34 12 73 195 206 84 96 89 57 24 10 44 117 159
Endoplasmic Reticulum 1 1 0 0 0 0 1 0 0 0 0 0 1 0 1 5 7 10 2 0 0 0 1 1
Endosome 0 1 0 9 3 2 0 0 0 1 0 0 2 3 0 21 16 26 14 6 2 3 3 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 4 2 1 0 0 0 0
Mitochondria 131 7 0 4 0 12 116 51 116 114 88 30 0 0 0 6 2 15 23 4 8 7 11 8
Nucleus 96 24 4 20 3 4 22 7 25 19 20 8 35 0 1 22 13 29 161 66 14 47 5 7
Nuclear Periphery 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 12 1 1 0 0 0
Nucleolus 1 1 0 0 0 0 0 0 1 1 0 0 0 0 0 1 1 2 3 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 2 1 1 2 2 0 2 0 0 0 1 0 2 1 2 22 5 4 11 8 17
Vac/Vac Membrane 101 90 16 33 21 16 28 7 14 14 7 5 52 39 10 128 137 143 114 39 16 48 15 16
Unique Cell Count 326 137 27 109 156 130 259 112 180 218 116 42 143 226 215 227 234 275 437 162 66 178 183 234
Labelled Cell Count 394 159 31 117 163 148 321 132 225 256 149 56 164 238 218 271 281 322 437 162 66 178 183 234


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 5.0 5.6 5.2 5.1 4.0 3.7 4.1 3.4 3.9 3.6 3.7 6.1 5.8 5.5 7.4 6.9 6.8 5.7 6.0 6.3
Std Deviation (1e-4) 0.5 0.7 0.8 1.5 1.4 1.5 1.2 1.0 1.2 1.6 1.8 1.3 1.1 1.3 1.0 1.8 1.9 1.9 1.7 2.3 2.1
Intensity Change (Log2) – – – -0.09 -0.14 -0.47 -0.6 -0.45 -0.7 -0.52 -0.63 -0.58 0.12 0.05 -0.02 0.41 0.31 0.28 0.03 0.11 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 5.5 5.1 1.5 1.6 -0.4 0.6 -1.2 -1.0 1.0 5.8 8.9 -0.4 0 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 1.1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.8 -5.5 -5.5 0 0 0 0 0 -4.2 -2.2 -5.0 0 -0.3 -0.1 -0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0379 0.1043 0.2276 -0.1079 -0.2279 0.1474 0.2235 0.6301 0.5586 0.944 0.4485 0.3915 0.1808 0.0448 -0.1631 0.0695 0.5108 -0.037
Actin 0.0066 0.0008 0.0047 0.003 0.0118 0.0007 0.0304 0.0005 0.0216 0.0035 0.0058 0.0005 0.0427 0.0017 0.0005 0.0108 0.0048 0.0003
Bud 0.0001 0.0018 0.0002 0.0003 0.0002 0 0.0006 0.0002 0.0017 0.0001 0.0007 0.0126 0.0009 0.0033 0.0001 0.0029 0.0015 0.0001
Bud Neck 0.0016 0.0008 0.0098 0.0014 0.0014 0.0017 0.0061 0.0007 0.0044 0.0012 0.0033 0.0023 0.0043 0.0007 0.0006 0.0309 0.0013 0.0022
Bud Periphery 0.0001 0.0005 0.0003 0.0003 0.0002 0 0.0007 0.0002 0.001 0.0001 0.0007 0.0029 0.0011 0.0004 0.0001 0.0031 0.0028 0.0001
Bud Site 0.0008 0.0051 0.0004 0.0006 0.0008 0.0001 0.0027 0.0027 0.0104 0.0003 0.0009 0.0006 0.0147 0.005 0.0007 0.0282 0.0053 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0002 0 0.0001 0.0001 0.0003 0.0002 0 0.0014 0.0003 0
Cytoplasm 0.2115 0.2402 0.1457 0.1835 0.1342 0.1188 0.1733 0.2594 0.2204 0.1813 0.164 0.141 0.3363 0.3066 0.2787 0.341 0.4569 0.2676
Cytoplasmic Foci 0.0053 0.0054 0.0058 0.0062 0.0129 0.0064 0.011 0.0038 0.0134 0.015 0.0217 0.007 0.0142 0.0164 0.0078 0.0075 0.0113 0.0059
Eisosomes 0.0001 0.0001 0.0004 0 0.0002 0.0002 0.0003 0 0.0001 0 0.0001 0 0.0004 0.0001 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0139 0.0063 0.0045 0.0063 0.0031 0.0027 0.0194 0.0064 0.005 0.0061 0.0092 0.0028 0.0129 0.0133 0.0129 0.0082 0.013 0.0052
Endosome 0.0134 0.0075 0.0047 0.0122 0.0165 0.0053 0.0251 0.0063 0.0068 0.0037 0.0122 0.0056 0.0173 0.0077 0.0075 0.0059 0.0308 0.0084
Golgi 0.0011 0.0002 0.0004 0.0006 0.0058 0.0001 0.0033 0.0005 0.0037 0.0014 0.0044 0.0003 0.0069 0.0008 0.0002 0.0002 0.0128 0.0001
Lipid Particles 0.0019 0.0005 0.0015 0.0008 0.0136 0.0007 0.0037 0.0006 0.0028 0.0062 0.0166 0.0009 0.0095 0.0006 0.0005 0.0002 0.0236 0.0003
Mitochondria 0.0014 0.0012 0.0013 0.0023 0.0014 0.0006 0.0017 0.0008 0.0038 0.0011 0.0037 0.0008 0.009 0.0024 0.0006 0.0008 0.0385 0.0006
None 0.3361 0.2491 0.3944 0.1776 0.5428 0.2209 0.1307 0.1829 0.1616 0.1566 0.1334 0.1488 0.0663 0.2048 0.0964 0.1176 0.0509 0.1399
Nuclear Periphery 0.0505 0.0313 0.033 0.0509 0.017 0.0164 0.0418 0.0261 0.0318 0.0251 0.0381 0.0139 0.0419 0.0465 0.0274 0.0276 0.0512 0.0219
Nucleolus 0.0038 0.0049 0.0029 0.0033 0.006 0.0062 0.0037 0.003 0.0034 0.0021 0.0053 0.0056 0.0017 0.0042 0.0021 0.0012 0.0022 0.0023
Nucleus 0.312 0.3921 0.3251 0.4832 0.1698 0.5423 0.4997 0.4798 0.4534 0.5318 0.4336 0.5902 0.3734 0.3424 0.5397 0.3974 0.26 0.5226
Peroxisomes 0.0004 0.0003 0.001 0.0005 0.0033 0.0011 0.0008 0.0003 0.0039 0.0184 0.0581 0.0005 0.0148 0.0008 0.0002 0.0001 0.0064 0.0001
Punctate Nuclear 0.0336 0.046 0.0619 0.0633 0.0571 0.0737 0.0334 0.0211 0.0485 0.044 0.0838 0.0595 0.025 0.0374 0.0213 0.0125 0.0142 0.0186
Vacuole 0.0045 0.0049 0.0015 0.0022 0.0013 0.0017 0.0092 0.0039 0.0017 0.0013 0.0032 0.0036 0.005 0.0039 0.0023 0.0019 0.0082 0.0029
Vacuole Periphery 0.001 0.0008 0.0006 0.0014 0.0005 0.0004 0.002 0.0005 0.0005 0.0005 0.0012 0.0005 0.0014 0.0009 0.0005 0.0005 0.004 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.0332 20.5495 22.1105 28.0355 18.6278 13.7849 24.4137 31.9034 24.1564 30.6756
Translational Efficiency 1.0471 1.4648 1.3173 0.9975 1.8804 2.9053 1.4363 1.0527 1.4902 1.3753

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein required for localizing proteasomes to the nucleus; involved in cotranslational protein degradation; mediates interaction between nuclear import factor Srp1p and the proteasome; Sts1p and Srp1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation; involved in ubiquitin-mediated protein degradation
Localization
Cell Percentages nucleus (46%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Sts1

Sts1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sts1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available