Standard name
Human Ortholog
Description bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex; participates in the regulation of sulfur metabolism
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.54 0.68 0.58 0.66 0.69 0.76 0.86 0.84 0.67 0.67 0.47 0.45 0.7 0.64 0.61 0.77 0.69 0.64 0.3 0.43 0.34 0.67 0.65 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.12 0 0 0.08 0.31 0.11 0.23 0.44 0.4 0.6 0.58 0 0 0 0.06 0 0 0.09 0 0.09 0.06 0.05 0
Nucleus 0.05 0.11 0.27 0.19 0.16 0.05 0 0 0 0 0.05 0 0.13 0.14 0.19 0.16 0.08 0.14 0.16 0.22 0.23 0.06 0.06 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0.3 0.17 0.15 0.17 0.18 0.05 0.1 0 0.05 0 0.06 0.07 0.21 0.23 0.22 0.1 0.24 0.2 0.29 0.21 0.23 0.11 0.11 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 3 5
Bud 0 0 0 0 0 2 1 2 2 1 5 3 0 0 0 1 0 0 1 5 1 5 10 10
Bud Neck 0 0 0 0 0 0 3 2 0 0 1 1 0 0 0 0 1 3 0 3 0 2 5 12
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 2 0 2 0 0 0 0 0 1
Cytoplasm 71 253 42 51 60 115 169 196 124 106 41 48 188 354 346 163 141 112 74 210 41 275 374 323
Endoplasmic Reticulum 1 1 0 1 1 0 0 0 0 0 0 0 2 1 0 10 4 10 2 0 1 4 2 5
Endosome 5 1 0 3 2 1 1 0 1 2 0 0 11 29 21 2 7 4 8 4 1 6 4 9
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 2
Mitochondria 17 44 2 3 7 47 21 54 82 64 52 62 4 5 5 13 0 5 22 14 10 26 27 22
Nucleus 7 42 20 15 14 7 4 6 6 2 4 1 34 78 108 33 16 25 40 105 27 24 37 27
Nuclear Periphery 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 5 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 1 0 2 0 1 5 1 0 1 1 1 2 0 2 0 0 0 11 19 2 11 25 21
Vac/Vac Membrane 39 65 11 13 16 7 19 9 9 7 5 7 55 128 126 21 49 35 71 102 27 44 60 66
Unique Cell Count 132 372 73 77 87 151 196 232 186 159 87 106 268 552 568 212 205 175 250 490 120 414 573 531
Labelled Cell Count 148 408 75 88 100 180 225 270 224 184 110 123 296 595 608 245 219 196 250 490 120 414 573 531


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.9 4.9 5.1 5.6 3.9 4.5 4.8 3.7 4.0 4.0 3.8 4.9 5.0 5.0 5.2 5.9 5.1 3.9 4.3 4.3
Std Deviation (1e-4) 0.7 0.6 1.0 2.4 2.1 1.8 1.9 2.5 1.5 1.5 1.9 1.5 1.0 1.2 1.0 0.9 1.4 1.2 0.9 1.2 1.3
Intensity Change (Log2) – – – 0.05 0.18 -0.36 -0.15 -0.05 -0.4 -0.3 -0.31 -0.36 0.0 0.03 0.03 0.07 0.27 0.04 -0.35 -0.2 -0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -1.2 1.6 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4292 0.0916 -0.0372 -0.305 -0.4524 0.0458 1.6777 2.195 2.2862 1.708 1.5073 1.9732 3.0234 3.7893 3.5734 4.7274 3.8752 3.9185
Actin 0.0001 0.0001 0.0035 0.0002 0.0002 0.0007 0.0001 0.0001 0 0 0.0001 0.0001 0.0194 0.0002 0.0104 0.0206 0.0144 0.0072
Bud 0.0001 0.0001 0.0001 0 0 0.0001 0.0002 0.0001 0 0 0 0 0.0005 0 0.0006 0.0003 0.0019 0.0001
Bud Neck 0.0005 0.0004 0.0019 0.0024 0.0007 0.0042 0.0004 0.0006 0.0004 0.0005 0.0008 0.0018 0.0068 0.0002 0.0014 0.0008 0.0018 0.005
Bud Periphery 0 0.0001 0.0001 0 0 0.0001 0.0001 0 0 0 0 0 0.0007 0 0.0011 0.0004 0.0034 0.0002
Bud Site 0.0002 0.0004 0.0022 0.0001 0.0001 0.0003 0.0002 0.0007 0.0002 0 0.0001 0.0001 0.0017 0.0003 0.0036 0.0015 0.0016 0.0003
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0002 0
Cytoplasm 0.0243 0.041 0.0193 0.0162 0.0113 0.016 0.0169 0.0587 0.0113 0.0119 0.0297 0.0147 0.0966 0.0624 0.0307 0.0807 0.0467 0.0188
Cytoplasmic Foci 0.0048 0.0013 0.0015 0.0005 0.0012 0.0009 0.0007 0.0018 0.0003 0.0001 0.0019 0.0011 0.0061 0.0004 0.0122 0.026 0.0031 0.0025
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.001 0.0012 0.0007 0.0002 0.0006 0.0003 0.0005 0.0008 0.0002 0.0002 0.0004 0.0003 0.0058 0.0031 0.0045 0.0052 0.0102 0.0009
Endosome 0.0017 0.0016 0.0012 0.0004 0.0019 0.0007 0.0009 0.0021 0.0008 0.0002 0.0016 0.0011 0.0125 0.0007 0.0078 0.0106 0.0164 0.0051
Golgi 0.0001 0 0.0001 0 0 0.0001 0 0 0 0 0.0001 0 0.0026 0 0.0023 0.0116 0.0015 0.0027
Lipid Particles 0.0004 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0 0 0.0001 0 0.0102 0.0001 0.0077 0.0154 0.0018 0.0029
Mitochondria 0.0003 0.0004 0.0002 0.0002 0.0003 0.0002 0.0002 0.0003 0.0001 0.0001 0.0002 0.0002 0.0034 0.0003 0.0046 0.0084 0.0051 0.0017
None 0.0575 0.0355 0.0461 0.063 0.0747 0.0249 0.0374 0.0502 0.0201 0.0267 0.0416 0.0144 0.0654 0.0963 0.0384 0.0873 0.0956 0.0295
Nuclear Periphery 0.0142 0.017 0.0086 0.0046 0.0216 0.0052 0.0069 0.0106 0.0049 0.0029 0.009 0.0039 0.0308 0.0124 0.0142 0.0046 0.0344 0.0082
Nucleolus 0.0091 0.0069 0.0056 0.0081 0.0108 0.0157 0.0118 0.0097 0.0088 0.0071 0.0214 0.0186 0.0145 0.0048 0.0058 0.0035 0.007 0.0095
Nucleus 0.8317 0.8536 0.8733 0.8457 0.8422 0.8534 0.8816 0.8188 0.91 0.9357 0.7665 0.8946 0.6837 0.8002 0.8154 0.6611 0.7227 0.854
Peroxisomes 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0 0 0.0001 0.0001 0.0105 0 0.0057 0.0332 0.001 0.0038
Punctate Nuclear 0.0529 0.0389 0.0348 0.0579 0.0335 0.0766 0.041 0.044 0.0418 0.0142 0.1257 0.0486 0.0222 0.0175 0.0308 0.0274 0.0269 0.0463
Vacuole 0.0007 0.001 0.0005 0.0002 0.0004 0.0003 0.0008 0.0012 0.0008 0.0002 0.0005 0.0004 0.0048 0.0008 0.0021 0.0009 0.0026 0.0007
Vacuole Periphery 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0014 0.0002 0.0008 0.0003 0.0015 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8256 3.4978 12.9775 17.6643 8.8779 15.6425 6.6311 15.5153 17.1322 9.7771
Translational Efficiency 1.6668 2.767 1.3655 1.8111 1.537 1.5989 2.3342 1.8449 1.6792 1.4104

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
174 952 167 159 1310 1488 1193 1528 1484 2440 1360 1687

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 604.75 696.37 865.21 892.05 648.07 756.66 806.72 815.29 642.99 733.14 813.90 822.52
Standard Deviation 59.85 96.01 91.12 99.96 76.49 90.84 95.57 106.11 76.02 97.44 96.96 107.91
Intensity Change Log 2 — 0.203515 0.516711 0.560786 — 0.223496 0.315918 0.331164 — 0.213885 0.416335 0.446571

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000186 0.000935 0.000573 0.000884 0.000107 0.000133 0.000259 0.000293 0.000116 0.000446 0.000298 0.000348
Bud Neck 0.009730 0.016207 0.002205 0.015550 0.004150 0.008763 0.002564 0.007892 0.004804 0.011667 0.002520 0.008614
Bud Site 0.001381 0.013562 0.001222 0.023666 0.001162 0.004284 0.002474 0.017912 0.001187 0.007904 0.002320 0.018454
Cell Periphery 0.000251 0.000225 0.000085 0.000254 0.000154 0.000169 0.000112 0.000194 0.000166 0.000191 0.000108 0.000199
Cytoplasm 0.215924* 0.259324* 0.031892 0.184476* 0.202297* 0.214606* 0.113856* 0.373267* 0.203894* 0.232053* 0.103791* 0.355473*
Cytoplasmic Foci 0.134027 0.067541 0.000778 0.003687 0.042906 0.035405 0.003161 0.007820 0.053590 0.047943 0.002868 0.007431
Eisosomes 0.000128 0.000074 0.000050 0.000061 0.000052 0.000036 0.000030 0.000031 0.000061 0.000051 0.000033 0.000033
Endoplasmic Reticulum 0.001443 0.004261 0.007835 0.008460 0.000971 0.001241 0.004065 0.002370 0.001027 0.002419 0.004528 0.002944
Endosome 0.003883 0.007724 0.000950 0.004467 0.001355 0.002249 0.001223 0.002538 0.001651 0.004385 0.001190 0.002720
Golgi 0.000562 0.002860 0.000050 0.000188 0.000912 0.001253 0.000235 0.000539 0.000871 0.001880 0.000212 0.000506
Lipid Particles 0.011491 0.006236 0.001099 0.001428 0.004399 0.004169 0.000983 0.000980 0.005230 0.004976 0.000997 0.001022
Mitochondria 0.005212 0.011850 0.000789 0.001025 0.003490 0.006719 0.000736 0.002429 0.003692 0.008721 0.000742 0.002297
Mitotic Spindle 0.000822 0.010213 0.000298 0.002924 0.003599 0.006089 0.001836 0.018609 0.003274 0.007698 0.001647 0.017131
None 0.013017 0.019339 0.002188 0.006054 0.012031 0.009658 0.004632 0.010648 0.012147 0.013435 0.004332 0.010215
Nuclear Periphery 0.000903 0.001556 0.001555 0.003216 0.001132 0.001582 0.001622 0.002447 0.001105 0.001572 0.001614 0.002519
Nuclear Periphery Foci 0.000959 0.001301 0.000474 0.003390 0.000688 0.000331 0.000592 0.001080 0.000720 0.000710 0.000578 0.001297
Nucleolus 0.007418 0.008248 0.001373 0.000660 0.003442 0.003867 0.000845 0.001055 0.003908 0.005577 0.000910 0.001018
Nucleus 0.573939* 0.535194* 0.928511* 0.654648* 0.710537* 0.689278* 0.845656* 0.514254* 0.694521* 0.629160* 0.855830* 0.527486*
Peroxisomes 0.008368 0.008936 0.000189 0.000677 0.002790 0.002826 0.000150 0.000621 0.003444 0.005210 0.000155 0.000626
Vacuole 0.009701 0.022643 0.017647 0.078496 0.003592 0.006413 0.014647 0.033906 0.004308 0.012745 0.015016 0.038109
Vacuole Periphery 0.000656 0.001772 0.000237 0.005790 0.000236 0.000928 0.000323 0.001116 0.000285 0.001258 0.000312 0.001556

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.30 -2.63 -3.33 1.13 0.81 -0.69 -8.06 -4.34 -3.99 0.22 -4.80 -7.11 -5.02 0.66 0.80
Bud Neck -2.26 2.16 -0.22 1.99 -2.44 -3.61 1.49 -3.68 0.49 -5.90 -6.21 2.28 -3.34 3.15 -6.40
Bud Site -6.37 -0.96 -2.15 0.50 -1.69 -2.96 -1.62 -6.55 -4.57 -5.73 -6.59 -1.83 -6.89 -2.70 -5.97
Cell Periphery 0.54 2.95 1.09 1.15 -3.38 -0.66 2.94 0.24 0.92 -2.92 -1.47 3.95 0.74 2.49 -3.64
Cytoplasm -2.30 10.02 3.79 7.70 -6.50 -1.46 12.51 -11.59 -10.29 -24.68 -3.52 14.88 -10.33 -7.71 -25.46
Cytoplasmic Foci 4.72 9.68 9.49 16.12 -4.29 2.32 14.05 12.72 12.11 -4.32 1.81 16.55 15.32 19.09 -4.78
Eisosomes 4.65 5.41 3.53 -1.06 -2.31 2.26 1.69 1.96 -0.97 0.62 1.48 2.80 2.87 3.33 0.05
Endoplasmic Reticulum -7.07 -8.97 -9.11 -6.30 -0.97 -2.00 -15.37 -12.71 -10.67 6.56 -8.59 -17.53 -14.95 -7.02 5.92
Endosome -3.29 3.50 0.82 4.15 -2.62 -1.81 0.40 -1.52 0.58 -1.85 -5.75 1.45 -1.01 4.92 -2.40
Golgi -3.78 4.43 3.97 4.75 -1.49 -0.47 1.67 1.37 1.90 -0.79 -1.79 1.83 1.50 4.09 -0.84
Lipid Particles 3.05 5.97 5.73 7.94 -1.75 0.56 6.01 6.43 6.00 1.64 0.68 7.82 8.15 10.08 1.32
Mitochondria -3.19 2.78 3.23 6.96 1.09 -2.16 2.65 1.58 4.29 -2.65 -3.84 3.09 2.04 7.21 -2.46
Mitotic Spindle -4.97 -0.37 -1.15 4.42 -0.88 -0.93 1.13 -4.30 -3.40 -5.77 -2.33 1.11 -4.40 -2.64 -5.83
None -1.65 3.71 3.00 6.79 -2.88 1.42 6.23 2.86 1.40 -5.22 -0.68 7.04 3.46 4.61 -5.57
Nuclear Periphery -4.28 -5.45 -5.64 -4.45 -3.25 -2.83 -8.76 -6.47 -4.54 -3.26 -4.15 -10.00 -7.55 -5.51 -3.88
Nuclear Periphery Foci -0.86 1.26 -1.87 -1.53 -2.78 1.86 -0.31 -1.08 -3.63 -1.16 0.03 -0.03 -1.51 -2.02 -2.01
Nucleolus -0.28 4.56 5.13 7.39 4.03 -0.31 6.68 6.92 6.79 0.21 -2.33 7.95 8.39 9.60 0.82
Nucleus 1.28 -12.37 -1.95 -3.88 10.41 1.61 -13.30 13.09 11.69 28.86 6.06 -16.55 11.64 6.36 30.79
Peroxisomes -0.04 4.97 4.91 7.99 -0.55 0.10 5.28 4.78 5.85 -2.01 -2.38 6.84 6.34 9.69 -2.06
Vacuole -6.28 -4.14 -9.27 -8.42 -7.22 -3.75 -11.40 -18.90 -17.82 -10.23 -9.77 -12.07 -20.44 -17.34 -12.05
Vacuole Periphery -4.10 1.90 -0.70 -0.06 -0.93 -2.34 -0.15 -2.33 0.75 -1.81 -4.80 0.20 -2.13 1.55 -2.01
Standard name
Human Ortholog
Description bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex; participates in the regulation of sulfur metabolism
Localization
Cell Percentages nucleus (78%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Met28

Met28


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met28-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available