Standard name | |
---|---|
Human Ortholog | |
Description | bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex; participates in the regulation of sulfur metabolism |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 3 | 5 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 1 | 5 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 1 | 5 | 10 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 3 | 0 | 2 | 5 | 12 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 71 | 253 | 42 | 51 | 60 | 115 | 169 | 196 | 124 | 106 | 41 | 48 | 188 | 354 | 346 | 163 | 141 | 112 | 74 | 210 | 41 | 275 | 374 | 323 |
Endoplasmic Reticulum | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 10 | 4 | 10 | 2 | 0 | 1 | 4 | 2 | 5 |
Endosome | 5 | 1 | 0 | 3 | 2 | 1 | 1 | 0 | 1 | 2 | 0 | 0 | 11 | 29 | 21 | 2 | 7 | 4 | 8 | 4 | 1 | 6 | 4 | 9 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 2 |
Mitochondria | 17 | 44 | 2 | 3 | 7 | 47 | 21 | 54 | 82 | 64 | 52 | 62 | 4 | 5 | 5 | 13 | 0 | 5 | 22 | 14 | 10 | 26 | 27 | 22 |
Nucleus | 7 | 42 | 20 | 15 | 14 | 7 | 4 | 6 | 6 | 2 | 4 | 1 | 34 | 78 | 108 | 33 | 16 | 25 | 40 | 105 | 27 | 24 | 37 | 27 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 1 | 0 | 2 | 0 | 1 | 5 | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 11 | 19 | 2 | 11 | 25 | 21 |
Vac/Vac Membrane | 39 | 65 | 11 | 13 | 16 | 7 | 19 | 9 | 9 | 7 | 5 | 7 | 55 | 128 | 126 | 21 | 49 | 35 | 71 | 102 | 27 | 44 | 60 | 66 |
Unique Cell Count | 132 | 372 | 73 | 77 | 87 | 151 | 196 | 232 | 186 | 159 | 87 | 106 | 268 | 552 | 568 | 212 | 205 | 175 | 250 | 490 | 120 | 414 | 573 | 531 |
Labelled Cell Count | 148 | 408 | 75 | 88 | 100 | 180 | 225 | 270 | 224 | 184 | 110 | 123 | 296 | 595 | 608 | 245 | 219 | 196 | 250 | 490 | 120 | 414 | 573 | 531 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 3.9 | 4.9 | 5.1 | 5.6 | 3.9 | 4.5 | 4.8 | 3.7 | 4.0 | 4.0 | 3.8 | 4.9 | 5.0 | 5.0 | 5.2 | 5.9 | 5.1 | 3.9 | 4.3 | 4.3 |
Std Deviation (1e-4) | 0.7 | 0.6 | 1.0 | 2.4 | 2.1 | 1.8 | 1.9 | 2.5 | 1.5 | 1.5 | 1.9 | 1.5 | 1.0 | 1.2 | 1.0 | 0.9 | 1.4 | 1.2 | 0.9 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.18 | -0.36 | -0.15 | -0.05 | -0.4 | -0.3 | -0.31 | -0.36 | 0.0 | 0.03 | 0.03 | 0.07 | 0.27 | 0.04 | -0.35 | -0.2 | -0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | -1.2 | 1.6 | 0.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.4292 | 0.0916 | -0.0372 | -0.305 | -0.4524 | 0.0458 | 1.6777 | 2.195 | 2.2862 | 1.708 | 1.5073 | 1.9732 | 3.0234 | 3.7893 | 3.5734 | 4.7274 | 3.8752 | 3.9185 |
Actin | 0.0001 | 0.0001 | 0.0035 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0194 | 0.0002 | 0.0104 | 0.0206 | 0.0144 | 0.0072 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0006 | 0.0003 | 0.0019 | 0.0001 |
Bud Neck | 0.0005 | 0.0004 | 0.0019 | 0.0024 | 0.0007 | 0.0042 | 0.0004 | 0.0006 | 0.0004 | 0.0005 | 0.0008 | 0.0018 | 0.0068 | 0.0002 | 0.0014 | 0.0008 | 0.0018 | 0.005 |
Bud Periphery | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0.0011 | 0.0004 | 0.0034 | 0.0002 |
Bud Site | 0.0002 | 0.0004 | 0.0022 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0007 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0017 | 0.0003 | 0.0036 | 0.0015 | 0.0016 | 0.0003 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 |
Cytoplasm | 0.0243 | 0.041 | 0.0193 | 0.0162 | 0.0113 | 0.016 | 0.0169 | 0.0587 | 0.0113 | 0.0119 | 0.0297 | 0.0147 | 0.0966 | 0.0624 | 0.0307 | 0.0807 | 0.0467 | 0.0188 |
Cytoplasmic Foci | 0.0048 | 0.0013 | 0.0015 | 0.0005 | 0.0012 | 0.0009 | 0.0007 | 0.0018 | 0.0003 | 0.0001 | 0.0019 | 0.0011 | 0.0061 | 0.0004 | 0.0122 | 0.026 | 0.0031 | 0.0025 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.001 | 0.0012 | 0.0007 | 0.0002 | 0.0006 | 0.0003 | 0.0005 | 0.0008 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0058 | 0.0031 | 0.0045 | 0.0052 | 0.0102 | 0.0009 |
Endosome | 0.0017 | 0.0016 | 0.0012 | 0.0004 | 0.0019 | 0.0007 | 0.0009 | 0.0021 | 0.0008 | 0.0002 | 0.0016 | 0.0011 | 0.0125 | 0.0007 | 0.0078 | 0.0106 | 0.0164 | 0.0051 |
Golgi | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0026 | 0 | 0.0023 | 0.0116 | 0.0015 | 0.0027 |
Lipid Particles | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0102 | 0.0001 | 0.0077 | 0.0154 | 0.0018 | 0.0029 |
Mitochondria | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0034 | 0.0003 | 0.0046 | 0.0084 | 0.0051 | 0.0017 |
None | 0.0575 | 0.0355 | 0.0461 | 0.063 | 0.0747 | 0.0249 | 0.0374 | 0.0502 | 0.0201 | 0.0267 | 0.0416 | 0.0144 | 0.0654 | 0.0963 | 0.0384 | 0.0873 | 0.0956 | 0.0295 |
Nuclear Periphery | 0.0142 | 0.017 | 0.0086 | 0.0046 | 0.0216 | 0.0052 | 0.0069 | 0.0106 | 0.0049 | 0.0029 | 0.009 | 0.0039 | 0.0308 | 0.0124 | 0.0142 | 0.0046 | 0.0344 | 0.0082 |
Nucleolus | 0.0091 | 0.0069 | 0.0056 | 0.0081 | 0.0108 | 0.0157 | 0.0118 | 0.0097 | 0.0088 | 0.0071 | 0.0214 | 0.0186 | 0.0145 | 0.0048 | 0.0058 | 0.0035 | 0.007 | 0.0095 |
Nucleus | 0.8317 | 0.8536 | 0.8733 | 0.8457 | 0.8422 | 0.8534 | 0.8816 | 0.8188 | 0.91 | 0.9357 | 0.7665 | 0.8946 | 0.6837 | 0.8002 | 0.8154 | 0.6611 | 0.7227 | 0.854 |
Peroxisomes | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0105 | 0 | 0.0057 | 0.0332 | 0.001 | 0.0038 |
Punctate Nuclear | 0.0529 | 0.0389 | 0.0348 | 0.0579 | 0.0335 | 0.0766 | 0.041 | 0.044 | 0.0418 | 0.0142 | 0.1257 | 0.0486 | 0.0222 | 0.0175 | 0.0308 | 0.0274 | 0.0269 | 0.0463 |
Vacuole | 0.0007 | 0.001 | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0008 | 0.0012 | 0.0008 | 0.0002 | 0.0005 | 0.0004 | 0.0048 | 0.0008 | 0.0021 | 0.0009 | 0.0026 | 0.0007 |
Vacuole Periphery | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0014 | 0.0002 | 0.0008 | 0.0003 | 0.0015 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.8256 | 3.4978 | 12.9775 | 17.6643 | 8.8779 | 15.6425 | 6.6311 | 15.5153 | 17.1322 | 9.7771 |
Translational Efficiency | 1.6668 | 2.767 | 1.3655 | 1.8111 | 1.537 | 1.5989 | 2.3342 | 1.8449 | 1.6792 | 1.4104 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|