Standard name
Human Ortholog
Description bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex; participates in the regulation of sulfur metabolism

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.54 0.68 0.58 0.66 0.69 0.76 0.86 0.84 0.67 0.67 0.47 0.45 0.7 0.64 0.61 0.77 0.69 0.64 0.3 0.43 0.34 0.67 0.65 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.12 0 0 0.08 0.31 0.11 0.23 0.44 0.4 0.6 0.58 0 0 0 0.06 0 0 0.09 0 0.09 0.06 0.05 0
Nucleus 0.05 0.11 0.27 0.19 0.16 0.05 0 0 0 0 0.05 0 0.13 0.14 0.19 0.16 0.08 0.14 0.16 0.22 0.23 0.06 0.06 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0.3 0.17 0.15 0.17 0.18 0.05 0.1 0 0.05 0 0.06 0.07 0.21 0.23 0.22 0.1 0.24 0.2 0.29 0.21 0.23 0.11 0.11 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 3 5
Bud 0 0 0 0 0 2 1 2 2 1 5 3 0 0 0 1 0 0 1 5 1 5 10 10
Bud Neck 0 0 0 0 0 0 3 2 0 0 1 1 0 0 0 0 1 3 0 3 0 2 5 12
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 2 0 2 0 0 0 0 0 1
Cytoplasm 71 253 42 51 60 115 169 196 124 106 41 48 188 354 346 163 141 112 74 210 41 275 374 323
Endoplasmic Reticulum 1 1 0 1 1 0 0 0 0 0 0 0 2 1 0 10 4 10 2 0 1 4 2 5
Endosome 5 1 0 3 2 1 1 0 1 2 0 0 11 29 21 2 7 4 8 4 1 6 4 9
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 2
Mitochondria 17 44 2 3 7 47 21 54 82 64 52 62 4 5 5 13 0 5 22 14 10 26 27 22
Nucleus 7 42 20 15 14 7 4 6 6 2 4 1 34 78 108 33 16 25 40 105 27 24 37 27
Nuclear Periphery 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 5 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 1 0 2 0 1 5 1 0 1 1 1 2 0 2 0 0 0 11 19 2 11 25 21
Vac/Vac Membrane 39 65 11 13 16 7 19 9 9 7 5 7 55 128 126 21 49 35 71 102 27 44 60 66
Unique Cell Count 132 372 73 77 87 151 196 232 186 159 87 106 268 552 568 212 205 175 250 490 120 414 573 531
Labelled Cell Count 148 408 75 88 100 180 225 270 224 184 110 123 296 595 608 245 219 196 250 490 120 414 573 531


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.9 4.9 5.1 5.6 3.9 4.5 4.8 3.7 4.0 4.0 3.8 4.9 5.0 5.0 5.2 5.9 5.1 3.9 4.3 4.3
Std Deviation (1e-4) 0.7 0.6 1.0 2.4 2.1 1.8 1.9 2.5 1.5 1.5 1.9 1.5 1.0 1.2 1.0 0.9 1.4 1.2 0.9 1.2 1.3
Intensity Change (Log2) 0.05 0.18 -0.36 -0.15 -0.05 -0.4 -0.3 -0.31 -0.36 0.0 0.03 0.03 0.07 0.27 0.04 -0.35 -0.2 -0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole -1.2 1.6 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4292 0.0916 -0.0372 -0.305 -0.4524 0.0458 1.6777 2.195 2.2862 1.708 1.5073 1.9732 3.0234 3.7893 3.5734 4.7274 3.8752 3.9185
Actin 0.0001 0.0001 0.0035 0.0002 0.0002 0.0007 0.0001 0.0001 0 0 0.0001 0.0001 0.0194 0.0002 0.0104 0.0206 0.0144 0.0072
Bud 0.0001 0.0001 0.0001 0 0 0.0001 0.0002 0.0001 0 0 0 0 0.0005 0 0.0006 0.0003 0.0019 0.0001
Bud Neck 0.0005 0.0004 0.0019 0.0024 0.0007 0.0042 0.0004 0.0006 0.0004 0.0005 0.0008 0.0018 0.0068 0.0002 0.0014 0.0008 0.0018 0.005
Bud Periphery 0 0.0001 0.0001 0 0 0.0001 0.0001 0 0 0 0 0 0.0007 0 0.0011 0.0004 0.0034 0.0002
Bud Site 0.0002 0.0004 0.0022 0.0001 0.0001 0.0003 0.0002 0.0007 0.0002 0 0.0001 0.0001 0.0017 0.0003 0.0036 0.0015 0.0016 0.0003
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0002 0
Cytoplasm 0.0243 0.041 0.0193 0.0162 0.0113 0.016 0.0169 0.0587 0.0113 0.0119 0.0297 0.0147 0.0966 0.0624 0.0307 0.0807 0.0467 0.0188
Cytoplasmic Foci 0.0048 0.0013 0.0015 0.0005 0.0012 0.0009 0.0007 0.0018 0.0003 0.0001 0.0019 0.0011 0.0061 0.0004 0.0122 0.026 0.0031 0.0025
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.001 0.0012 0.0007 0.0002 0.0006 0.0003 0.0005 0.0008 0.0002 0.0002 0.0004 0.0003 0.0058 0.0031 0.0045 0.0052 0.0102 0.0009
Endosome 0.0017 0.0016 0.0012 0.0004 0.0019 0.0007 0.0009 0.0021 0.0008 0.0002 0.0016 0.0011 0.0125 0.0007 0.0078 0.0106 0.0164 0.0051
Golgi 0.0001 0 0.0001 0 0 0.0001 0 0 0 0 0.0001 0 0.0026 0 0.0023 0.0116 0.0015 0.0027
Lipid Particles 0.0004 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0 0 0.0001 0 0.0102 0.0001 0.0077 0.0154 0.0018 0.0029
Mitochondria 0.0003 0.0004 0.0002 0.0002 0.0003 0.0002 0.0002 0.0003 0.0001 0.0001 0.0002 0.0002 0.0034 0.0003 0.0046 0.0084 0.0051 0.0017
None 0.0575 0.0355 0.0461 0.063 0.0747 0.0249 0.0374 0.0502 0.0201 0.0267 0.0416 0.0144 0.0654 0.0963 0.0384 0.0873 0.0956 0.0295
Nuclear Periphery 0.0142 0.017 0.0086 0.0046 0.0216 0.0052 0.0069 0.0106 0.0049 0.0029 0.009 0.0039 0.0308 0.0124 0.0142 0.0046 0.0344 0.0082
Nucleolus 0.0091 0.0069 0.0056 0.0081 0.0108 0.0157 0.0118 0.0097 0.0088 0.0071 0.0214 0.0186 0.0145 0.0048 0.0058 0.0035 0.007 0.0095
Nucleus 0.8317 0.8536 0.8733 0.8457 0.8422 0.8534 0.8816 0.8188 0.91 0.9357 0.7665 0.8946 0.6837 0.8002 0.8154 0.6611 0.7227 0.854
Peroxisomes 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0 0 0.0001 0.0001 0.0105 0 0.0057 0.0332 0.001 0.0038
Punctate Nuclear 0.0529 0.0389 0.0348 0.0579 0.0335 0.0766 0.041 0.044 0.0418 0.0142 0.1257 0.0486 0.0222 0.0175 0.0308 0.0274 0.0269 0.0463
Vacuole 0.0007 0.001 0.0005 0.0002 0.0004 0.0003 0.0008 0.0012 0.0008 0.0002 0.0005 0.0004 0.0048 0.0008 0.0021 0.0009 0.0026 0.0007
Vacuole Periphery 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0014 0.0002 0.0008 0.0003 0.0015 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8256 3.4978 12.9775 17.6643 8.8779 15.6425 6.6311 15.5153 17.1322 9.7771
Translational Efficiency 1.6668 2.767 1.3655 1.8111 1.537 1.5989 2.3342 1.8449 1.6792 1.4104

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex; participates in the regulation of sulfur metabolism
Localization
Cell Percentages nucleus (78%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Met28

Met28


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met28-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available