Standard name
Human Ortholog
Description 18kDa catalytic subunit of the Signal Peptidase Complex (SPC); the Signal Peptidase Complex cleaves the signal sequence of proteins targeted to the endoplasmic reticulum; other members are Spc1p, Spc2p, Spc3p, and Sec11p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.1 0.06 0.07 0.06 0.05 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0.07 0.07 0.05 0.09 0.15 – – – 0 0 0 0 0 0
Cytoplasm 0.08 0.12 0.12 0.18 0.22 0.26 0.25 0.32 – 0.24 0.32 0.27 0.11 0.11 0.12 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0.86 0.85 0.85 0.62 0.57 0.53 0.39 0.31 – 0.34 0.22 0.36 0.86 0.86 0.78 – – – 0.91 0.91 0.91 0.84 0.81 0.81
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0.13 0.17 0.17 0.16 0.23 0.23 – 0.31 0.43 0.35 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0.07 0.06 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.06 0 0.08 0.08 0.08 0.14 0.16 – 0.2 0.09 0.09 0 0 0 – – – 0 0 0 0.05 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 13 8 12 19 15 23 13 – 15 3 13 6 12 20 – – – 2 2 1 2 4 5
Bud 1 3 1 1 6 6 25 14 – 11 10 12 1 3 1 – – – 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 1 0 0 1 1 1
Bud Site 0 0 0 0 0 0 0 0 – 0 1 1 0 0 0 – – – – – – – – –
Cell Periphery 8 7 3 6 9 5 8 14 – 15 16 26 15 38 70 – – – 0 0 0 0 1 2
Cytoplasm 20 35 10 36 65 68 111 121 – 92 71 99 32 48 57 – – – 4 5 1 2 6 9
Endoplasmic Reticulum 227 241 71 124 166 140 176 116 – 131 50 129 252 369 371 – – – 256 282 97 153 262 309
Endosome 0 0 1 1 7 0 11 9 – 1 0 2 0 0 0 – – – 1 0 0 0 2 2
Golgi 0 0 1 3 1 0 0 0 – 0 0 0 0 0 1 – – – 2 3 0 1 2 4
Mitochondria 1 0 11 35 50 41 102 85 – 121 96 127 0 1 0 – – – 1 1 2 6 12 15
Nucleus 2 0 0 1 0 0 3 4 – 0 4 3 2 1 3 – – – 0 0 0 0 0 0
Nuclear Periphery 15 6 6 11 4 10 16 6 – 14 5 4 5 15 9 – – – 1 6 2 3 2 2
Nucleolus 0 0 0 1 0 2 2 1 – 0 1 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 – 0 0 0 0 1 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 26 16 3 16 24 21 63 60 – 79 21 31 5 10 9 – – – 6 4 1 8 24 26
Unique Cell Count 263 283 84 200 290 263 448 373 386 224 362 292 428 476 281 309 107 182 325 384
Labelled Cell Count 309 321 115 247 351 308 540 443 479 278 447 318 498 541 281 309 107 182 325 384


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 11.9 8.1 8.1 7.7 7.0 6.6 7.0 – 6.4 6.6 6.6 10.1 10.2 10.3 – – – 10.7 11.1 11.4
Std Deviation (1e-4) 1.5 1.7 1.3 1.4 1.3 1.3 1.3 1.6 – 1.2 1.4 1.3 1.2 1.4 1.3 – – – 1.8 1.8 1.8
Intensity Change (Log2) – – – 0.01 -0.06 -0.2 -0.29 -0.2 – -0.32 -0.29 -0.28 0.33 0.35 0.36 – – – 0.42 0.47 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.1 -0.9 -1.2 0 0 – 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 – 0 1.2 1.2 0 1.6 2.8 – – –
Cytoplasm 1.3 2.1 2.7 2.6 3.8 – 2.4 3.5 3.0 -0.2 -0.2 0 – – –
Endoplasmic Reticulum -3.7 -4.6 -5.1 -7.6 -9.0 – -8.5 -10.0 -8.1 0.4 0.4 -1.4 – – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Mitochondria 0.9 0.9 0.6 2.0 2.0 – 3.4 4.9 3.9 0 0 0 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery -0.5 0 0 0 0 – 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Vacuole 1.4 1.5 1.4 2.7 3.0 – 3.7 1.7 1.6 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.153 6.1733 5.7784 5.3343 5.2662 6.0512 5.6879 6.9779 6.2828 6.3259 5.5306 6.3128 4.8978 4.9607 4.7502 4.3803 4.2061 4.5209
Actin 0.0121 0.0043 0.0176 0.0026 0.0006 0.001 0.0375 0.0004 0.0258 0.025 0.0009 0.0032 0.0078 0.0001 0.0105 0.0157 0.0158 0.0015
Bud 0.0036 0.006 0 0.0003 0 0 0.0002 0.0005 0.0006 0.0001 0 0 0.0001 0 0.0001 0.0003 0.0002 0
Bud Neck 0.0001 0.0003 0.0003 0.0042 0.0007 0.0007 0.001 0.0002 0.0058 0.0008 0.0001 0.0008 0.0007 0.0001 0.0003 0.0004 0.0004 0.0002
Bud Periphery 0.002 0.0014 0.0001 0.0004 0 0.0001 0.0004 0.0002 0.0007 0.0002 0 0.0001 0.0004 0 0.0001 0.0006 0.0004 0
Bud Site 0.0019 0.001 0.0002 0.0024 0.0004 0.0002 0.0018 0.002 0.0018 0.0006 0 0.0001 0.0006 0.0001 0.0008 0.0008 0.0036 0
Cell Periphery 0.0004 0.0011 0.0006 0.0005 0.0003 0.0004 0.0006 0.0012 0.0007 0.0011 0.0003 0.0007 0.0005 0.0004 0.0002 0.0005 0.0002 0.0001
Cytoplasm 0.0182 0.0751 0.0414 0.0445 0.1025 0.0778 0.0082 0.0746 0.0257 0.0519 0.0482 0.0421 0.0193 0.0442 0.0383 0.0242 0.0132 0.025
Cytoplasmic Foci 0.0033 0.0046 0.0035 0.0106 0.002 0.0049 0.0141 0.0063 0.0056 0.0046 0.0014 0.0021 0.0095 0.001 0.002 0.0075 0.0062 0.0018
Eisosomes 0.0002 0.0001 0.0001 0.0001 0 0 0.0008 0.0001 0.0002 0.0008 0 0 0.0001 0 0.0001 0.0003 0.0001 0
Endoplasmic Reticulum 0.8655 0.8454 0.8692 0.8507 0.8225 0.8519 0.8077 0.8682 0.8649 0.8805 0.8997 0.9062 0.8788 0.8987 0.9186 0.8498 0.8914 0.9183
Endosome 0.0198 0.0174 0.0203 0.0196 0.009 0.0125 0.0324 0.0065 0.0107 0.0055 0.0097 0.0101 0.0168 0.0099 0.0033 0.0438 0.0151 0.0088
Golgi 0.0016 0.0013 0.0015 0.0069 0.0004 0.0014 0.0157 0.0009 0.0054 0.0056 0.0003 0.0048 0.0039 0.0002 0.0025 0.0079 0.0023 0.0023
Lipid Particles 0.0085 0.0071 0.0056 0.0167 0.0009 0.0043 0.0171 0.0085 0.0066 0.0099 0.0036 0.0031 0.0078 0.0026 0.0045 0.012 0.0085 0.0076
Mitochondria 0.0035 0.0003 0.0005 0.0014 0.0003 0.0016 0.0041 0.0032 0.0152 0.0017 0.0001 0.0009 0.0026 0.0001 0.0016 0.0023 0.0005 0.0005
None 0.0073 0.0034 0.0033 0.0007 0.0025 0.0013 0.0061 0.0029 0.0015 0.001 0.0008 0.0123 0.0014 0.0003 0.0004 0.0019 0.0012 0.0002
Nuclear Periphery 0.029 0.0203 0.0229 0.018 0.032 0.0202 0.0344 0.0098 0.0079 0.0045 0.025 0.0072 0.0256 0.0265 0.0102 0.0219 0.0282 0.0219
Nucleolus 0.0004 0.0001 0.0001 0.0012 0.0001 0.0008 0.0001 0.0015 0.0002 0 0 0 0.0004 0 0 0.0001 0.0002 0
Nucleus 0.0054 0.0026 0.002 0.0026 0.0168 0.0048 0.0016 0.0029 0.0042 0.0003 0.0029 0.0007 0.0023 0.0037 0.0005 0.0014 0.0017 0.0021
Peroxisomes 0.0052 0.0002 0.0019 0.0057 0 0.0024 0.0066 0.0001 0.0092 0.0021 0 0.0004 0.003 0 0.0034 0.0009 0.0045 0.0045
Punctate Nuclear 0.0013 0.001 0.0012 0.0052 0.0004 0.0058 0.0022 0.0046 0.0035 0.0003 0.0007 0.0001 0.0041 0.0002 0.0001 0.0009 0.0019 0.0004
Vacuole 0.0074 0.0066 0.0069 0.0045 0.0074 0.0072 0.0046 0.0046 0.0031 0.0032 0.0055 0.0045 0.0112 0.0113 0.002 0.0058 0.0036 0.0038
Vacuole Periphery 0.0034 0.0006 0.0008 0.0008 0.001 0.0006 0.0027 0.0011 0.0008 0.0003 0.0006 0.0004 0.0029 0.0006 0.0003 0.0011 0.0006 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.3127 41.099 40.663 51.4341 48.2548 48.1378 58.8698 52.0872 61.3497 56.9166
Translational Efficiency 2.1899 1.9234 1.634 1.3544 1.55 1.9786 1.4918 1.6743 1.3996 1.3477

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
910 1292 924 102 1322 1599 1839 1555 2232 2891 2763 1657

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 802.20 1032.95 1250.52 1398.96 865.98 915.77 1329.98 1249.26 839.98 968.14 1303.41 1258.48
Standard Deviation 133.54 167.23 197.91 179.04 209.48 160.79 175.87 209.27 185.05 173.76 187.32 210.63
Intensity Change Log 2 — 0.364737 0.640494 0.802321 — 0.080652 0.618999 0.528668 — 0.224252 0.629376 0.666748

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004712 0.000945 0.000789 0.004669 0.001940 0.001252 0.000652 0.003131 0.003070 0.001115 0.000698 0.003225
Bud Neck 0.000409 0.000673 0.000444 0.003827 0.001660 0.000914 0.000226 0.001888 0.001150 0.000807 0.000299 0.002007
Bud Site 0.004186 0.003995 0.001843 0.009954 0.007662 0.004583 0.001867 0.014551 0.006245 0.004320 0.001859 0.014268
Cell Periphery 0.006490 0.001059 0.000570 0.002440 0.001444 0.001748 0.000451 0.002873 0.003501 0.001440 0.000491 0.002846
Cytoplasm 0.021085 0.031187 0.013971 0.019930 0.055447 0.042921 0.011388 0.034176 0.041437 0.037677 0.012252 0.033299
Cytoplasmic Foci 0.008409 0.008665 0.001792 0.002582 0.027088 0.020433 0.000565 0.007361 0.019472 0.015174 0.000975 0.007067
Eisosomes 0.001802 0.000150 0.000019 0.000133 0.000502 0.000364 0.000030 0.000180 0.001032 0.000268 0.000026 0.000177
Endoplasmic Reticulum 0.904019* 0.892967* 0.922944* 0.807291* 0.761841* 0.837844* 0.954046* 0.875294* 0.819808* 0.862478* 0.943645* 0.871108*
Endosome 0.004409 0.007896 0.009834 0.015303 0.013156 0.011445 0.004769 0.012332 0.009590 0.009859 0.006463 0.012515
Golgi 0.007155 0.006612 0.001608 0.000617 0.016865 0.017473 0.000783 0.005048 0.012906 0.012619 0.001059 0.004776
Lipid Particles 0.008991 0.012515 0.002797 0.013295 0.033223 0.021889 0.002078 0.011667 0.023344 0.017700 0.002318 0.011767
Mitochondria 0.008881 0.004136 0.000332 0.000106 0.005109 0.006443 0.000215 0.002079 0.006647 0.005412 0.000254 0.001957
Mitotic Spindle 0.000094 0.000238 0.000405 0.007412 0.000147 0.000099 0.000254 0.000866 0.000125 0.000161 0.000305 0.001269
None 0.003009 0.003365 0.000267 0.000033 0.016049 0.006180 0.001013 0.002697 0.010732 0.004922 0.000763 0.002533
Nuclear Periphery 0.001742 0.003336 0.006043 0.010733 0.007504 0.002271 0.005026 0.000577 0.005155 0.002747 0.005366 0.001203
Nuclear Periphery Foci 0.005164 0.012630 0.026884 0.063659 0.021241 0.009874 0.013516 0.009369 0.014686 0.011106 0.017987 0.012710
Nucleolus 0.000554 0.000059 0.000060 0.000019 0.003626 0.000504 0.000004 0.000047 0.002374 0.000305 0.000023 0.000045
Nucleus 0.002134 0.001399 0.000820 0.006099 0.014148 0.002975 0.000600 0.001704 0.009250 0.002270 0.000674 0.001975
Peroxisomes 0.004494 0.001416 0.000032 0.000055 0.002909 0.002974 0.000074 0.000925 0.003555 0.002278 0.000060 0.000872
Vacuole 0.001217 0.004587 0.007861 0.030710 0.005789 0.004780 0.002327 0.010614 0.003925 0.004694 0.004178 0.011851
Vacuole Periphery 0.001044 0.002171 0.000685 0.001133 0.002650 0.003034 0.000117 0.002622 0.001995 0.002649 0.000307 0.002531

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.75 2.83 -0.05 -1.45 -1.50 1.32 2.47 -1.56 -2.79 -3.64 3.06 3.68 -0.29 -3.28 -3.87
Bud Neck -0.95 -0.38 -3.42 -3.12 -3.38 2.07 4.55 -0.65 -3.06 -6.10 1.44 4.36 -2.83 -4.14 -6.55
Bud Site 0.15 2.46 -1.41 -1.45 -1.91 2.10 4.04 -3.10 -4.98 -6.39 1.93 4.61 -4.16 -5.39 -6.76
Cell Periphery 4.02 4.39 2.22 -1.11 -1.51 -0.70 4.30 -3.41 -2.18 -6.30 3.41 5.29 0.94 -3.40 -6.47
Cytoplasm -3.28 2.49 -0.26 1.21 -1.27 3.24 13.28 5.12 2.31 -8.85 1.45 12.80 2.29 1.18 -8.70
Cytoplasmic Foci -0.11 4.38 3.42 4.43 -0.92 2.47 11.97 8.24 6.76 -7.45 2.48 12.67 7.34 5.88 -7.07
Eisosomes 2.82 3.05 2.82 0.16 -1.18 0.70 2.59 1.72 2.23 -4.78 2.88 3.84 3.23 1.64 -5.12
Endoplasmic Reticulum 1.12 -1.96 3.47 3.16 4.10 -6.49 -18.71 -9.55 -3.74 11.62 -5.35 -17.05 -5.32 -0.67 11.08
Endosome -4.60 -6.05 -3.36 -2.34 -1.74 1.39 7.37 0.54 -0.77 -6.52 -0.29 4.00 -2.60 -2.51 -5.61
Golgi 0.39 4.47 5.71 7.36 1.89 -0.29 10.14 6.74 7.37 -5.37 0.19 11.10 6.38 6.74 -4.96
Lipid Particles -1.48 3.71 -1.01 -0.38 -2.19 3.66 11.86 7.51 4.57 -7.70 2.76 12.29 5.59 3.21 -7.88
Mitochondria 2.54 5.38 5.53 4.06 1.86 -1.13 5.83 3.06 4.50 -3.98 1.20 7.83 4.95 4.33 -3.87
Mitotic Spindle -2.05 -1.40 -1.61 -1.58 -1.53 1.87 -0.94 -2.47 -2.60 -2.01 -1.04 -1.69 -2.95 -2.87 -2.37
None -0.25 2.86 3.10 3.39 3.56 3.77 6.16 5.29 2.73 -2.14 3.54 6.61 5.02 2.40 -2.49
Nuclear Periphery -1.76 -4.59 -2.40 -1.97 -1.23 3.72 1.67 5.50 2.62 5.85 2.64 -0.25 4.76 3.01 6.92
Nuclear Periphery Foci -5.40 -8.25 -4.61 -4.03 -2.87 4.79 2.98 4.86 0.22 2.39 2.40 -1.86 0.99 -1.16 2.85
Nucleolus 1.49 1.48 1.57 2.76 0.78 3.93 4.74 4.68 1.91 -4.34 4.25 4.95 4.90 1.95 -1.17
Nucleus 1.13 1.88 -1.01 -1.22 -1.36 5.62 7.15 6.49 1.83 -4.35 5.78 7.37 5.96 0.39 -3.92
Peroxisomes 3.93 6.63 6.59 3.34 -1.24 -0.07 6.18 3.81 3.97 -3.32 2.63 9.05 5.95 3.61 -3.39
Vacuole -4.41 -4.82 -3.24 -2.89 -2.50 1.08 4.29 -3.79 -4.79 -7.05 -1.23 -0.62 -6.27 -5.72 -5.87
Vacuole Periphery -2.02 1.49 -0.12 1.15 -0.59 -0.59 6.06 0.03 0.51 -3.98 -1.46 6.38 -0.83 0.17 -3.74
Standard name
Human Ortholog
Description 18kDa catalytic subunit of the Signal Peptidase Complex (SPC); the Signal Peptidase Complex cleaves the signal sequence of proteins targeted to the endoplasmic reticulum; other members are Spc1p, Spc2p, Spc3p, and Sec11p
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Sec11

Sec11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available