Standard name | |
---|---|
Human Ortholog | |
Description | Alpha subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 14 | 34 | 40 | 39 | 50 | 69 | 73 | 75 | 86 | 92 | 99 | 9 | 18 | 12 | 4 | 6 | 2 | 0 | 3 | 22 | 10 | 20 | 17 |
Bud | 2 | 3 | 1 | 1 | 1 | 2 | 2 | 6 | 4 | 3 | 6 | 8 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 8 | 40 | 20 | 20 | 37 | 48 | 50 | 38 | 60 | 58 | 83 | 12 | 39 | 52 | 5 | 9 | 7 | 1 | 1 | 8 | 11 | 23 | 26 |
Cytoplasm | 5 | 28 | 13 | 17 | 22 | 36 | 43 | 18 | 23 | 20 | 20 | 31 | 15 | 31 | 30 | 5 | 12 | 4 | 0 | 3 | 0 | 2 | 6 | 5 |
Endoplasmic Reticulum | 181 | 240 | 448 | 245 | 274 | 392 | 481 | 582 | 276 | 346 | 306 | 291 | 184 | 364 | 334 | 0 | 0 | 0 | 186 | 243 | 438 | 274 | 401 | 482 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 2 | 1 |
Golgi | 10 | 4 | 101 | 119 | 131 | 53 | 53 | 60 | 46 | 39 | 35 | 72 | 12 | 7 | 3 | 5 | 14 | 9 | 0 | 2 | 7 | 13 | 20 | 8 |
Mitochondria | 1 | 0 | 13 | 38 | 21 | 53 | 80 | 84 | 101 | 96 | 142 | 106 | 0 | 0 | 0 | 3 | 3 | 2 | 1 | 3 | 4 | 6 | 8 | 6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 12 | 4 | 2 | 8 | 6 | 19 | 15 | 9 | 20 | 10 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 0 | 2 | 0 | 12 | 14 | 9 | 0 | 3 | 3 | 6 | 9 | 0 | 4 | 5 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 7 | 4 |
Unique Cell Count | 190 | 261 | 493 | 319 | 376 | 494 | 608 | 683 | 403 | 464 | 457 | 463 | 204 | 412 | 387 | 16 | 37 | 21 | 192 | 267 | 490 | 326 | 497 | 563 |
Labelled Cell Count | 217 | 297 | 664 | 484 | 523 | 647 | 791 | 893 | 582 | 662 | 686 | 709 | 237 | 465 | 437 | 22 | 45 | 24 | 192 | 267 | 490 | 326 | 497 | 563 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 30.1 | 30.1 | 28.5 | 27.8 | 27.8 | 25.1 | 23.6 | 22.9 | 21.4 | 21.4 | 21.3 | 21.8 | 24.7 | 26.5 | 26.6 | 25.1 | 26.0 | 36.1 | 26.5 | 26.9 | 29.7 |
Std Deviation (1e-4) | 4.5 | 5.7 | 4.6 | 4.5 | 4.6 | 3.8 | 3.8 | 4.2 | 3.9 | 3.9 | 3.8 | 4.2 | 4.8 | 5.2 | 4.4 | 6.6 | 8.7 | 6.8 | 5.5 | 5.7 | 5.7 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.04 | -0.19 | -0.28 | -0.32 | -0.42 | -0.41 | -0.42 | -0.39 | -0.21 | -0.11 | -0.1 | -0.19 | -0.14 | 0.34 | -0.11 | -0.08 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.7 | 1.8 | 1.8 | 2.5 | 2.2 | 5.3 | 5.4 | 6.0 | 6.5 | -1.2 | -1.6 | -2.5 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.0 | -1.6 | -0.4 | -0.1 | -0.5 | 0.7 | 2.4 | 2.3 | 4.5 | -1.0 | 0.7 | 2.6 | 0 | 0 | 0 |
Cytoplasm | 2.0 | 2.4 | 3.4 | 3.3 | 0 | 2.3 | 1.4 | 1.5 | 3.0 | 2.9 | 3.4 | 3.5 | 0 | 0 | 0 |
Endoplasmic Reticulum | -5.5 | -7.0 | -5.1 | -5.3 | -2.9 | -8.5 | -6.7 | -9.1 | -10.3 | -0.3 | -1.2 | -2.1 | -11.0 | -14.7 | -12.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 5.3 | 4.7 | -4.2 | -5.6 | -5.8 | -3.6 | -5.3 | -5.6 | -2.0 | -4.8 | -8.7 | -9.0 | 0 | 2.5 | 0 |
Mitochondria | 5.3 | 2.2 | 5.1 | 6.2 | 5.9 | 10.0 | 8.8 | 11.9 | 9.5 | 0 | -3.3 | -3.2 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.2 | -2.2 | -0.9 | -1.9 | 0.4 | 1.1 | -0.5 | 1.7 | -0.3 | 0 | -2.4 | -3.1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.2 | 3.0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 24.4976 | 27.9048 | 25.6518 | 24.2243 | 21.8504 | 26.9929 | 29.8486 | 33.0831 | 31.4956 | 29.3488 | 29.3854 | 30.5534 | 30.8113 | 32.7315 | 30.0832 | 31.2862 | 29.8429 | 30.7795 |
Actin | 0.0095 | 0.0023 | 0.0175 | 0.0003 | 0.0032 | 0.0019 | 0.0048 | 0.0004 | 0.0054 | 0.0014 | 0.0278 | 0.0023 | 0.0143 | 0.0017 | 0.0023 | 0.0002 | 0.006 | 0.0004 |
Bud | 0.0005 | 0.0003 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0006 | 0.0004 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0002 |
Bud Periphery | 0.001 | 0.0008 | 0.001 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 |
Bud Site | 0.001 | 0.0005 | 0.0025 | 0.0001 | 0.0001 | 0 | 0.0006 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0027 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0058 | 0.0178 | 0.0135 | 0.0016 | 0.0127 | 0.0022 | 0.0049 | 0.0077 | 0.0064 | 0.0052 | 0.001 | 0.0017 | 0.0047 | 0.0062 | 0.0107 | 0.0072 | 0.0036 | 0.0011 |
Cytoplasm | 0.0269 | 0.0186 | 0.012 | 0.0016 | 0.0049 | 0.0108 | 0.0022 | 0.0029 | 0.0021 | 0.0004 | 0.0005 | 0.0004 | 0.0017 | 0.004 | 0.0014 | 0.0004 | 0.0009 | 0.0005 |
Cytoplasmic Foci | 0.006 | 0.0013 | 0.0057 | 0.0048 | 0.004 | 0.0006 | 0.0071 | 0.0006 | 0.0013 | 0.0002 | 0.0003 | 0.0004 | 0.01 | 0.0011 | 0.0096 | 0.0001 | 0.0043 | 0.0007 |
Eisosomes | 0.0004 | 0.0012 | 0.0017 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.003 | 0.0007 | 0.0008 | 0.0002 | 0.0005 | 0.0016 | 0.0006 | 0.0003 | 0.0012 | 0.001 | 0.0001 |
Endoplasmic Reticulum | 0.833 | 0.9224 | 0.8654 | 0.9492 | 0.815 | 0.9525 | 0.9264 | 0.9639 | 0.9654 | 0.9491 | 0.9523 | 0.9792 | 0.839 | 0.9114 | 0.9473 | 0.9558 | 0.8736 | 0.9574 |
Endosome | 0.0169 | 0.0019 | 0.0091 | 0.0109 | 0.022 | 0.0032 | 0.0053 | 0.0009 | 0.0018 | 0.0006 | 0.0021 | 0.001 | 0.0051 | 0.0036 | 0.0021 | 0.0009 | 0.0023 | 0.0036 |
Golgi | 0.0074 | 0.0079 | 0.0084 | 0.0082 | 0.0179 | 0.0047 | 0.0058 | 0.0077 | 0.0029 | 0.0017 | 0.008 | 0.0052 | 0.0052 | 0.0476 | 0.0025 | 0.0217 | 0.018 | 0.0067 |
Lipid Particles | 0.0121 | 0.0122 | 0.0138 | 0.0098 | 0.0216 | 0.0018 | 0.005 | 0.0055 | 0.0018 | 0.0043 | 0.0012 | 0.0018 | 0.0077 | 0.0068 | 0.0033 | 0.0016 | 0.0021 | 0.0034 |
Mitochondria | 0.0088 | 0.0011 | 0.0148 | 0.0015 | 0.0065 | 0.0064 | 0.0074 | 0.0009 | 0.003 | 0.0248 | 0.0013 | 0.0007 | 0.0167 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0022 |
None | 0.0025 | 0.0002 | 0.0059 | 0.0011 | 0.0009 | 0.0001 | 0.0011 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0032 | 0.0001 | 0.0008 | 0 | 0.0002 | 0 |
Nuclear Periphery | 0.0406 | 0.0065 | 0.0127 | 0.0046 | 0.0407 | 0.0121 | 0.0196 | 0.0039 | 0.004 | 0.0075 | 0.0032 | 0.0042 | 0.0661 | 0.0132 | 0.0125 | 0.0089 | 0.006 | 0.0212 |
Nucleolus | 0.0008 | 0.0001 | 0.0006 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0017 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0045 | 0.0005 | 0.0018 | 0.0003 | 0.0146 | 0.0007 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0029 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Peroxisomes | 0.0021 | 0 | 0.003 | 0.0005 | 0.0223 | 0 | 0.0015 | 0 | 0.0007 | 0.0006 | 0.0003 | 0.0009 | 0.0022 | 0 | 0.0009 | 0 | 0.0786 | 0.0001 |
Punctate Nuclear | 0.003 | 0.0001 | 0.0022 | 0.0004 | 0.0011 | 0.0001 | 0.0033 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.004 | 0 | 0.003 | 0 | 0.0003 | 0 |
Vacuole | 0.0107 | 0.0036 | 0.0045 | 0.0032 | 0.0046 | 0.0012 | 0.0019 | 0.0014 | 0.0025 | 0.0016 | 0.0005 | 0.001 | 0.0038 | 0.002 | 0.0018 | 0.0009 | 0.0007 | 0.0007 |
Vacuole Periphery | 0.0059 | 0.0005 | 0.0027 | 0.0011 | 0.0065 | 0.001 | 0.0016 | 0.0008 | 0.0011 | 0.0012 | 0.0002 | 0.0004 | 0.0057 | 0.0008 | 0.0005 | 0.0006 | 0.0009 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 212.816 | 173.7015 | 146.7959 | 169.9639 | 160.9564 | 174.1659 | 182.5988 | 167.2232 | 150.1472 | 185.662 |
Translational Efficiency | 0.7751 | 0.8686 | 0.8879 | 0.8117 | 0.8551 | 0.9266 | 0.7654 | 0.6598 | 0.7157 | 0.6635 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
91 | 1498 | 357 | 961 | 1890 | 1933 | 183 | 1619 | 1981 | 3431 | 540 | 2580 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2195.37 | 2176.98 | 3000.97 | 2823.54 | 1632.26 | 1997.66 | 2934.73 | 2714.28 | 1658.13 | 2075.95 | 2978.52 | 2754.98 |
Standard Deviation | 357.18 | 395.59 | 430.84 | 511.68 | 302.07 | 335.45 | 369.92 | 440.11 | 326.82 | 373.67 | 412.40 | 471.02 |
Intensity Change Log 2 | — | -0.012136 | 0.450965 | 0.363041 | — | 0.291440 | 0.846357 | 0.733699 | — | 0.125200 | 0.632967 | 0.532867 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000628 | 0.001004 | 0.000786 | 0.001050 | 0.000857 | 0.000799 | 0.000102 | 0.000673 | 0.000846 | 0.000889 | 0.000554 | 0.000814 |
Bud Neck | 0.003296 | 0.000224 | 0.005556 | 0.001261 | 0.000129 | 0.000157 | 0.000563 | 0.000453 | 0.000275 | 0.000187 | 0.003864 | 0.000754 |
Bud Site | 0.006846 | 0.000551 | 0.001031 | 0.002639 | 0.001070 | 0.000977 | 0.000232 | 0.000980 | 0.001336 | 0.000791 | 0.000760 | 0.001598 |
Cell Periphery | 0.008868 | 0.002256 | 0.010033 | 0.017090 | 0.003901 | 0.002350 | 0.007862 | 0.007090 | 0.004130 | 0.002309 | 0.009297 | 0.010815 |
Cytoplasm | 0.001005 | 0.000175 | 0.000168 | 0.000292 | 0.000603 | 0.000325 | 0.000009 | 0.000297 | 0.000622 | 0.000260 | 0.000114 | 0.000295 |
Cytoplasmic Foci | 0.009990 | 0.000412 | 0.000069 | 0.000848 | 0.001912 | 0.001276 | 0.000025 | 0.000230 | 0.002283 | 0.000899 | 0.000054 | 0.000461 |
Eisosomes | 0.000154 | 0.000622 | 0.001342 | 0.000882 | 0.000493 | 0.000458 | 0.001479 | 0.000304 | 0.000478 | 0.000529 | 0.001389 | 0.000519 |
Endoplasmic Reticulum | 0.856179* | 0.970039* | 0.895364* | 0.919645* | 0.964944* | 0.967759* | 0.962488* | 0.949098* | 0.959948* | 0.968754* | 0.918112* | 0.938127* |
Endosome | 0.019588 | 0.004918 | 0.008380 | 0.005759 | 0.005456 | 0.006554 | 0.003312 | 0.005896 | 0.006105 | 0.005840 | 0.006662 | 0.005845 |
Golgi | 0.033028 | 0.010419 | 0.008710 | 0.014888 | 0.010349 | 0.008484 | 0.008427 | 0.009949 | 0.011391 | 0.009329 | 0.008614 | 0.011788 |
Lipid Particles | 0.022486 | 0.003936 | 0.011778 | 0.013466 | 0.004604 | 0.007341 | 0.003579 | 0.011852 | 0.005426 | 0.005854 | 0.008999 | 0.012453 |
Mitochondria | 0.001275 | 0.000634 | 0.000494 | 0.000930 | 0.000896 | 0.000232 | 0.000163 | 0.000229 | 0.000913 | 0.000407 | 0.000382 | 0.000490 |
Mitotic Spindle | 0.000302 | 0.000021 | 0.000069 | 0.000373 | 0.000102 | 0.000028 | 0.000017 | 0.000070 | 0.000112 | 0.000025 | 0.000052 | 0.000183 |
None | 0.000040 | 0.000157 | 0.000036 | 0.000284 | 0.000689 | 0.000227 | 0.000023 | 0.000641 | 0.000660 | 0.000196 | 0.000031 | 0.000508 |
Nuclear Periphery | 0.001766 | 0.000451 | 0.021802 | 0.003534 | 0.000400 | 0.000265 | 0.003939 | 0.001658 | 0.000462 | 0.000346 | 0.015748 | 0.002357 |
Nuclear Periphery Foci | 0.006126 | 0.001092 | 0.011499 | 0.002782 | 0.000605 | 0.000786 | 0.001222 | 0.002539 | 0.000858 | 0.000920 | 0.008016 | 0.002630 |
Nucleolus | 0.000015 | 0.000003 | 0.000023 | 0.000016 | 0.000006 | 0.000002 | 0.000006 | 0.000005 | 0.000006 | 0.000002 | 0.000017 | 0.000009 |
Nucleus | 0.000068 | 0.000041 | 0.000104 | 0.000109 | 0.000049 | 0.000020 | 0.000021 | 0.000088 | 0.000050 | 0.000029 | 0.000076 | 0.000096 |
Peroxisomes | 0.001228 | 0.000046 | 0.000003 | 0.000034 | 0.000270 | 0.000214 | 0.000002 | 0.000028 | 0.000314 | 0.000140 | 0.000003 | 0.000030 |
Vacuole | 0.016692 | 0.001941 | 0.018669 | 0.009579 | 0.001119 | 0.001073 | 0.005059 | 0.006341 | 0.001835 | 0.001452 | 0.014057 | 0.007547 |
Vacuole Periphery | 0.010419 | 0.001056 | 0.004083 | 0.004539 | 0.001544 | 0.000674 | 0.001471 | 0.001580 | 0.001951 | 0.000841 | 0.003198 | 0.002682 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.89 | -0.37 | -0.82 | -0.22 | -0.52 | 0.19 | 2.80 | -0.01 | -0.18 | -2.27 | -0.14 | 0.80 | -0.32 | -0.24 | -1.06 |
Bud Neck | 2.26 | -1.36 | 1.40 | -3.41 | 3.98 | -0.71 | -3.85 | -3.42 | -3.14 | 0.03 | 1.19 | -5.26 | -3.63 | -4.53 | 4.36 |
Bud Site | 2.23 | 2.05 | 1.38 | -2.79 | -2.08 | 0.21 | 2.55 | -0.24 | -0.42 | -2.74 | 1.43 | 1.49 | -0.94 | -2.40 | -2.47 |
Cell Periphery | 3.18 | -0.49 | -2.98 | -7.99 | -3.07 | 3.27 | -2.52 | -4.20 | -6.68 | 0.41 | 4.15 | -4.70 | -7.53 | -10.28 | -1.16 |
Cytoplasm | 1.10 | 1.09 | 0.92 | -1.27 | -1.03 | 0.88 | 1.87 | 0.83 | -0.13 | -4.25 | 1.18 | 1.60 | 0.96 | -0.96 | -2.56 |
Cytoplasmic Foci | 1.87 | 1.94 | 1.78 | -1.30 | -2.42 | 1.32 | 4.84 | 4.28 | 3.73 | -4.54 | 2.94 | 5.05 | 3.97 | 2.15 | -3.30 |
Eisosomes | -1.97 | -3.46 | -3.49 | -0.86 | 1.17 | 0.14 | -1.78 | 1.46 | 0.66 | 2.15 | -0.26 | -2.94 | -0.30 | 0.05 | 2.90 |
Endoplasmic Reticulum | -3.91 | -1.24 | -2.13 | 8.65 | -2.43 | -0.87 | 0.54 | 4.45 | 5.02 | 2.80 | -2.89 | 6.48 | 6.07 | 9.52 | -2.90 |
Endosome | 3.06 | 2.31 | 2.87 | -1.38 | 3.32 | -1.99 | 4.45 | -0.91 | 0.81 | -4.31 | 0.52 | -1.10 | 0.31 | -0.18 | 1.40 |
Golgi | 1.91 | 2.05 | 1.53 | -2.77 | -3.31 | 1.57 | 1.62 | 0.32 | -1.26 | -1.30 | 1.82 | 2.12 | -0.35 | -2.61 | -2.78 |
Lipid Particles | 3.05 | 1.53 | 1.42 | -5.12 | -0.39 | -2.87 | 0.45 | -5.49 | -3.06 | -3.58 | -0.56 | -1.85 | -6.26 | -5.72 | -1.41 |
Mitochondria | 0.80 | 1.12 | 0.38 | -0.63 | -1.28 | 2.33 | 2.83 | 2.08 | -0.50 | -1.25 | 1.64 | 2.09 | 1.22 | -0.63 | -1.08 |
Mitotic Spindle | 2.65 | 2.19 | -0.45 | -2.63 | -2.27 | 2.08 | 2.41 | 0.44 | -1.77 | -2.14 | 2.53 | 1.71 | -1.27 | -3.09 | -2.58 |
None | -2.00 | 0.24 | -2.64 | -1.23 | -2.70 | 1.78 | 2.60 | 0.09 | -1.14 | -1.70 | 1.87 | 2.56 | 0.42 | -1.36 | -2.07 |
Nuclear Periphery | 1.75 | -6.69 | -2.15 | -11.93 | 6.30 | 2.64 | -7.78 | -8.53 | -9.19 | 4.73 | 1.92 | -7.82 | -13.69 | -14.69 | 6.84 |
Nuclear Periphery Foci | 2.26 | -1.94 | 1.47 | -2.44 | 4.70 | -0.74 | -2.57 | -3.46 | -2.87 | -2.27 | -0.37 | -6.02 | -4.18 | -3.67 | 4.28 |
Nucleolus | 1.71 | -1.00 | -0.23 | -3.07 | 0.99 | 3.62 | 0.10 | 0.12 | -3.07 | 0.03 | 3.36 | -2.90 | -1.56 | -4.09 | 1.89 |
Nucleus | 0.74 | -1.25 | -1.51 | -2.07 | -0.15 | 3.55 | 3.54 | -1.65 | -2.22 | -2.21 | 1.46 | -1.54 | -2.27 | -2.75 | -1.25 |
Peroxisomes | 1.07 | 1.11 | 1.08 | 0.46 | -3.01 | 0.59 | 3.75 | 3.35 | 2.84 | -2.68 | 1.87 | 3.66 | 3.33 | 2.86 | -3.79 |
Vacuole | 3.38 | -0.50 | 1.55 | -6.52 | 3.36 | 0.10 | -5.20 | -6.24 | -6.26 | -1.63 | 0.84 | -7.07 | -8.04 | -8.80 | 3.41 |
Vacuole Periphery | 1.39 | 0.94 | 0.86 | -4.07 | -0.43 | 2.85 | 0.22 | -0.23 | -3.45 | -0.49 | 2.64 | -1.92 | -1.50 | -5.12 | 0.76 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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