Standard name | |
---|---|
Human Ortholog | |
Description | Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 0 | 3 | 2 | 10 | 2 | – | 3 | 0 | 3 | 2 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 10 | 4 | 4 | 2 | 5 | 5 |
Bud | 1 | 2 | 7 | 18 | 16 | 18 | – | 11 | 25 | 36 | 46 | 35 | 4 | 1 | 0 | 0 | 0 | 0 | 25 | 4 | 6 | 5 | 21 | 7 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 12 | 4 | 3 | 0 | 3 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 1 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 10 | 15 | 18 | – | 6 | 2 | 4 | 6 | 5 | 4 | 5 | 4 | 1 | 2 | 2 | 1 | 0 | 1 | 0 | 1 | 1 |
Cytoplasm | 557 | 169 | 268 | 347 | 374 | 575 | – | 890 | 702 | 684 | 512 | 617 | 425 | 504 | 628 | 137 | 145 | 141 | 185 | 56 | 79 | 39 | 134 | 125 |
Endoplasmic Reticulum | 37 | 16 | 14 | 17 | 19 | 18 | – | 2 | 1 | 5 | 2 | 2 | 32 | 28 | 4 | 5 | 7 | 9 | 106 | 31 | 51 | 44 | 67 | 75 |
Endosome | 3 | 0 | 0 | 1 | 6 | 5 | – | 13 | 6 | 10 | 4 | 3 | 5 | 0 | 1 | 7 | 4 | 9 | 9 | 3 | 2 | 2 | 6 | 9 |
Golgi | 2 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 4 | 2 | 0 | 0 | 2 | 4 |
Mitochondria | 11 | 1 | 7 | 8 | 1 | 5 | – | 24 | 55 | 70 | 99 | 129 | 0 | 2 | 1 | 4 | 1 | 4 | 13 | 5 | 14 | 6 | 12 | 16 |
Nucleus | 0 | 1 | 1 | 0 | 0 | 3 | – | 2 | 1 | 0 | 0 | 1 | 2 | 1 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 1 | – | 13 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 1 | 1 | 0 | – | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | – | 7 | 1 | 6 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 3 |
Vac/Vac Membrane | 53 | 15 | 2 | 3 | 10 | 20 | – | 37 | 18 | 18 | 11 | 18 | 10 | 1 | 4 | 58 | 67 | 71 | 314 | 100 | 152 | 70 | 202 | 276 |
Unique Cell Count | 646 | 198 | 294 | 376 | 414 | 614 | 962 | 750 | 752 | 582 | 703 | 467 | 531 | 639 | 187 | 201 | 203 | 692 | 220 | 322 | 173 | 465 | 538 | |
Labelled Cell Count | 672 | 207 | 305 | 407 | 453 | 665 | 1012 | 813 | 837 | 685 | 821 | 484 | 544 | 645 | 217 | 226 | 240 | 692 | 220 | 322 | 173 | 465 | 538 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 6.4 | 4.9 | 5.5 | 6.9 | 5.4 | – | 4.7 | 3.9 | 4.2 | 3.9 | 4.0 | 6.2 | 5.6 | 5.0 | 8.0 | 7.8 | 7.9 | 6.7 | 6.6 | 6.0 |
Std Deviation (1e-4) | 2.9 | 2.5 | 2.6 | 2.8 | 2.2 | 1.7 | – | 1.3 | 0.8 | 0.6 | 0.6 | 1.3 | 2.6 | 1.6 | 1.3 | 2.6 | 3.2 | 3.0 | 3.6 | 3.1 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.5 | 0.14 | – | -0.04 | -0.33 | -0.23 | -0.32 | -0.3 | 0.35 | 0.21 | 0.04 | 0.71 | 0.67 | 0.7 | 0.46 | 0.43 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 1.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1.6 | 1.1 | 0.5 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 2.9 | 2.6 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | -0.4 | 1.4 | – | 0.8 | 1.4 | -0.1 | -1.4 | -1.5 | -0.1 | 2.1 | 5.2 | -5.2 | -5.6 | -6.2 |
Endoplasmic Reticulum | -0.1 | -0.1 | -1.4 | – | 0 | 0 | 0 | 0 | 0 | 1.2 | 0.3 | -4.3 | -1.1 | -0.7 | -0.2 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 2.4 | – | 2.7 | 1.8 | 1.8 | 0 | 1.9 | 0 | 0 | 0 | 9.8 | 10.3 | 10.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.381 | 1.0255 | 0.7976 | 0.5954 | 0.6488 | 0.6621 | -0.3034 | -0.021 | -0.0193 | 0.4305 | 0.2038 | -0.121 | 1.9721 | 2.543 | 2.3301 | 2.9566 | 2.1808 | 1.9677 |
Actin | 0.0102 | 0.0004 | 0.01 | 0.0001 | 0.0108 | 0.0059 | 0.0266 | 0.0002 | 0.0072 | 0.0436 | 0.0605 | 0.0018 | 0.0161 | 0.0007 | 0.0121 | 0.0018 | 0.0288 | 0.004 |
Bud | 0.0005 | 0.0003 | 0.0027 | 0.0002 | 0.0046 | 0.0019 | 0.0005 | 0.0038 | 0.0048 | 0.001 | 0.0013 | 0.0002 | 0.0002 | 0.0005 | 0.0007 | 0.0009 | 0.001 | 0.0007 |
Bud Neck | 0.0151 | 0.0089 | 0.0012 | 0.0004 | 0.0026 | 0.0035 | 0.0093 | 0.0003 | 0.0018 | 0.0012 | 0.0015 | 0.0031 | 0.0003 | 0.001 | 0.0066 | 0.0017 | 0.0036 | 0.003 |
Bud Periphery | 0.0005 | 0.0004 | 0.004 | 0.0004 | 0.0028 | 0.0041 | 0.0009 | 0.001 | 0.0036 | 0.0014 | 0.0028 | 0.0003 | 0.0002 | 0.0008 | 0.0017 | 0.0012 | 0.0019 | 0.0014 |
Bud Site | 0.0029 | 0.0121 | 0.0063 | 0.0002 | 0.0029 | 0.0023 | 0.0105 | 0.0027 | 0.009 | 0.0025 | 0.0021 | 0.0004 | 0.0009 | 0.0107 | 0.0126 | 0.0491 | 0.0048 | 0.0011 |
Cell Periphery | 0.0005 | 0.001 | 0.0007 | 0.0002 | 0.0012 | 0.0004 | 0.0011 | 0.0015 | 0.0008 | 0.0005 | 0.0005 | 0.0002 | 0.0004 | 0.0024 | 0.0018 | 0.0009 | 0.0005 | 0.0006 |
Cytoplasm | 0.3119 | 0.4282 | 0.3684 | 0.3357 | 0.3421 | 0.4842 | 0.2614 | 0.3953 | 0.4698 | 0.3451 | 0.277 | 0.5061 | 0.2993 | 0.4431 | 0.4654 | 0.3645 | 0.4254 | 0.5443 |
Cytoplasmic Foci | 0.0507 | 0.0252 | 0.052 | 0.0369 | 0.0883 | 0.048 | 0.0619 | 0.0205 | 0.0381 | 0.0302 | 0.0467 | 0.0515 | 0.0402 | 0.0272 | 0.027 | 0.0353 | 0.0357 | 0.0177 |
Eisosomes | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0005 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.017 | 0.0237 | 0.0163 | 0.0104 | 0.0179 | 0.0129 | 0.0299 | 0.0429 | 0.0215 | 0.0239 | 0.0169 | 0.0181 | 0.0403 | 0.0693 | 0.0444 | 0.088 | 0.017 | 0.0336 |
Endosome | 0.0623 | 0.0254 | 0.0781 | 0.0885 | 0.1038 | 0.0391 | 0.0799 | 0.0475 | 0.0753 | 0.0996 | 0.149 | 0.0675 | 0.0541 | 0.0489 | 0.0606 | 0.0835 | 0.0704 | 0.0432 |
Golgi | 0.0045 | 0.0008 | 0.0029 | 0.0008 | 0.01 | 0.0015 | 0.0089 | 0.0008 | 0.004 | 0.0084 | 0.0185 | 0.0019 | 0.0092 | 0.001 | 0.0014 | 0.0012 | 0.0071 | 0.0008 |
Lipid Particles | 0.0093 | 0.004 | 0.0083 | 0.0035 | 0.0483 | 0.0041 | 0.0286 | 0.0086 | 0.0103 | 0.0111 | 0.011 | 0.0048 | 0.0263 | 0.0032 | 0.0075 | 0.01 | 0.0178 | 0.0015 |
Mitochondria | 0.0035 | 0.0011 | 0.0017 | 0.0023 | 0.0087 | 0.002 | 0.0054 | 0.0005 | 0.0045 | 0.0042 | 0.0171 | 0.0013 | 0.008 | 0.0006 | 0.0046 | 0.0009 | 0.0074 | 0.001 |
None | 0.4392 | 0.4201 | 0.3719 | 0.4283 | 0.1862 | 0.329 | 0.3927 | 0.4011 | 0.2676 | 0.2778 | 0.2585 | 0.2629 | 0.4482 | 0.3127 | 0.3109 | 0.2816 | 0.2244 | 0.2807 |
Nuclear Periphery | 0.0112 | 0.0028 | 0.0078 | 0.0115 | 0.0213 | 0.0121 | 0.0101 | 0.0099 | 0.0075 | 0.0306 | 0.023 | 0.0079 | 0.0103 | 0.0087 | 0.0047 | 0.0067 | 0.0051 | 0.0045 |
Nucleolus | 0.0021 | 0.0015 | 0.0016 | 0.001 | 0.0064 | 0.0011 | 0.0011 | 0.0006 | 0.0009 | 0.0031 | 0.0007 | 0.0003 | 0.0003 | 0.0004 | 0.0007 | 0.0008 | 0.0016 | 0.0002 |
Nucleus | 0.0087 | 0.0059 | 0.0085 | 0.0053 | 0.0137 | 0.0078 | 0.0037 | 0.005 | 0.0123 | 0.0173 | 0.038 | 0.0115 | 0.0072 | 0.0041 | 0.0046 | 0.0047 | 0.0074 | 0.0058 |
Peroxisomes | 0.0058 | 0.0005 | 0.0013 | 0.0005 | 0.0261 | 0.0024 | 0.0081 | 0.0001 | 0.0053 | 0.0051 | 0.0087 | 0.0012 | 0.0167 | 0.0004 | 0.001 | 0.0007 | 0.0619 | 0.0003 |
Punctate Nuclear | 0.0091 | 0.004 | 0.0065 | 0.0187 | 0.0273 | 0.0047 | 0.0074 | 0.0017 | 0.003 | 0.014 | 0.0056 | 0.0023 | 0.0034 | 0.0021 | 0.0013 | 0.0023 | 0.0106 | 0.001 |
Vacuole | 0.0322 | 0.0323 | 0.0459 | 0.0471 | 0.0704 | 0.0303 | 0.0482 | 0.054 | 0.0472 | 0.0725 | 0.0518 | 0.0529 | 0.017 | 0.0594 | 0.028 | 0.0605 | 0.0632 | 0.0519 |
Vacuole Periphery | 0.0027 | 0.001 | 0.0036 | 0.0079 | 0.0046 | 0.0026 | 0.0035 | 0.0019 | 0.0054 | 0.0062 | 0.0083 | 0.0037 | 0.0014 | 0.0029 | 0.0021 | 0.0036 | 0.0042 | 0.0024 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 263.625 | 251.3756 | 262.9014 | 319.476 | 226.8768 | 212.8198 | 313.7933 | 323.3555 | 336.1179 | 363.3228 |
Translational Efficiency | 0.9999 | 1.2446 | 1.1748 | 1.0747 | 1.1275 | 1.1779 | 1.1551 | 0.9179 | 0.9588 | 0.9712 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
27 | 1258 | 208 | 834 | 1918 | 1401 | 1772 | 1415 | 1945 | 2659 | 1980 | 2249 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1702.63 | 812.50 | 987.18 | 1134.50 | 1256.22 | 822.50 | 886.05 | 1119.86 | 1262.42 | 817.77 | 896.67 | 1125.29 |
Standard Deviation | 2307.62 | 131.89 | 120.68 | 167.86 | 298.30 | 131.72 | 115.51 | 187.34 | 405.46 | 131.89 | 120.13 | 180.50 |
Intensity Change Log 2 | — | -1.067325 | -0.786380 | -0.585708 | — | -0.611002 | -0.503629 | -0.165771 | — | -0.855746 | -0.659509 | -0.392319 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.017678 | 0.000627 | 0.001073 | 0.007612 | 0.000727 | 0.000604 | 0.000855 | 0.005170 | 0.000962 | 0.000615 | 0.000878 | 0.006076 |
Bud Neck | 0.007283 | 0.019680 | 0.006424 | 0.007938 | 0.001302 | 0.022745 | 0.013744 | 0.008364 | 0.001385 | 0.021295 | 0.012975 | 0.008206 |
Bud Site | 0.087672 | 0.023110 | 0.007597 | 0.026912 | 0.012122 | 0.017119 | 0.024209 | 0.028050 | 0.013171 | 0.019953 | 0.022464 | 0.027628 |
Cell Periphery | 0.038792 | 0.001805 | 0.000621 | 0.002319 | 0.078714 | 0.003972 | 0.000833 | 0.004158 | 0.078160 | 0.002947 | 0.000810 | 0.003476 |
Cytoplasm | 0.257881* | 0.352559* | 0.215675* | 0.099897 | 0.037995 | 0.326807* | 0.420956* | 0.141372* | 0.041047 | 0.338991* | 0.399391* | 0.125992* |
Cytoplasmic Foci | 0.067851 | 0.191381* | 0.025408 | 0.028703 | 0.014027 | 0.165731 | 0.060385 | 0.024055 | 0.014775 | 0.177867 | 0.056711 | 0.025779 |
Eisosomes | 0.002111 | 0.000226 | 0.000163 | 0.000583 | 0.000427 | 0.000441 | 0.000305 | 0.000885 | 0.000450 | 0.000340 | 0.000290 | 0.000773 |
Endoplasmic Reticulum | 0.252087* | 0.012292 | 0.027282 | 0.432723* | 0.766360* | 0.030372 | 0.076011 | 0.548320* | 0.759221* | 0.021818 | 0.070892 | 0.505453* |
Endosome | 0.021417 | 0.043638 | 0.010239 | 0.037141 | 0.011248 | 0.043515 | 0.012212 | 0.024704 | 0.011390 | 0.043573 | 0.012005 | 0.029316 |
Golgi | 0.010959 | 0.003795 | 0.000187 | 0.006375 | 0.003607 | 0.005597 | 0.001553 | 0.009845 | 0.003709 | 0.004744 | 0.001410 | 0.008558 |
Lipid Particles | 0.034476 | 0.013099 | 0.008229 | 0.053631 | 0.009825 | 0.022473 | 0.024492 | 0.049686 | 0.010167 | 0.018038 | 0.022784 | 0.051149 |
Mitochondria | 0.001276 | 0.003315 | 0.000410 | 0.001150 | 0.001213 | 0.006121 | 0.001438 | 0.002601 | 0.001214 | 0.004794 | 0.001330 | 0.002063 |
Mitotic Spindle | 0.000440 | 0.008226 | 0.000678 | 0.004480 | 0.000080 | 0.003932 | 0.007052 | 0.003589 | 0.000085 | 0.005964 | 0.006383 | 0.003919 |
None | 0.007121 | 0.001427 | 0.001925 | 0.000683 | 0.001605 | 0.001006 | 0.001669 | 0.001998 | 0.001682 | 0.001205 | 0.001696 | 0.001511 |
Nuclear Periphery | 0.000506 | 0.000804 | 0.003531 | 0.004038 | 0.000908 | 0.001078 | 0.009270 | 0.001263 | 0.000902 | 0.000949 | 0.008667 | 0.002292 |
Nuclear Periphery Foci | 0.011733 | 0.001889 | 0.017579 | 0.055187 | 0.002254 | 0.001284 | 0.023188 | 0.030695 | 0.002386 | 0.001570 | 0.022599 | 0.039777 |
Nucleolus | 0.000861 | 0.001551 | 0.000587 | 0.000535 | 0.000106 | 0.001420 | 0.000717 | 0.000297 | 0.000117 | 0.001482 | 0.000703 | 0.000385 |
Nucleus | 0.108475 | 0.054730 | 0.444101* | 0.019870 | 0.001026 | 0.060845 | 0.190704* | 0.019224 | 0.002518 | 0.057952 | 0.217323* | 0.019463 |
Peroxisomes | 0.002959 | 0.008081 | 0.000680 | 0.001097 | 0.006084 | 0.005903 | 0.002244 | 0.001408 | 0.006041 | 0.006934 | 0.002080 | 0.001293 |
Vacuole | 0.065408 | 0.254811* | 0.227321* | 0.205257* | 0.045679 | 0.275926* | 0.127377 | 0.092379 | 0.045953 | 0.265936* | 0.137876 | 0.134238 |
Vacuole Periphery | 0.003013 | 0.002954 | 0.000288 | 0.003868 | 0.004690 | 0.003107 | 0.000788 | 0.001935 | 0.004666 | 0.003035 | 0.000735 | 0.002652 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.14 | 2.09 | 1.26 | -3.94 | -3.68 | 0.61 | -0.81 | -6.37 | -6.52 | -6.20 | 1.73 | 0.31 | -6.51 | -7.07 | -6.70 |
Bud Neck | -3.59 | -0.06 | -0.62 | 5.89 | -1.33 | -13.69 | -10.55 | -9.53 | 7.99 | 3.47 | -17.79 | -10.99 | -12.81 | 9.86 | 3.41 |
Bud Site | 1.75 | 2.18 | 1.61 | -1.27 | -6.36 | -2.09 | -4.44 | -6.03 | -4.06 | -1.85 | -3.07 | -3.61 | -6.49 | -3.78 | -2.53 |
Cell Periphery | 2.06 | 2.14 | 2.04 | -0.60 | -5.93 | 23.49* | 25.75* | 24.47 | -0.27 | -9.66 | 24.63 | 25.83 | 24.86 | -0.85 | -10.86 |
Cytoplasm | -1.54 | 1.38 | 2.87 | 25.88 | 6.16 | -41.24* | -55.40* | -19.08 | 21.88 | 32.86* | -53.27* | -53.36 | -19.33 | 33.03 | 36.07 |
Cytoplasmic Foci | -5.64 | 2.13 | 1.72 | 30.77 | -3.09 | -33.75* | -18.66 | -5.12 | 31.06 | 13.95 | -46.27 | -17.69 | -6.36 | 43.85 | 13.34 |
Eisosomes | 2.09 | 2.17 | 1.70 | -2.38 | -2.89 | -0.15 | 1.95 | -4.05 | -4.47 | -6.21 | 1.68 | 2.51 | -3.05 | -5.30 | -6.03 |
Endoplasmic Reticulum | 3.40 | 3.19 | -2.17 | -28.89 | -27.64* | 101.71* | 94.63* | 20.68 | -47.28* | -43.00* | 108.33 | 95.02 | 27.87 | -55.09* | -47.24* |
Endosome | -3.49 | 1.84 | -2.30 | 2.38 | -10.53 | -16.49 | -0.93 | -8.21 | 8.25 | -8.09 | -20.01 | -0.52 | -11.72 | 7.83 | -12.37 |
Golgi | 2.46 | 3.81 | 1.66 | -1.57 | -4.95 | -2.13 | 4.68 | -4.72 | -2.67 | -6.59 | -1.60 | 5.41 | -4.69 | -3.37 | -7.59 |
Lipid Particles | 1.67 | 2.15 | -1.51 | -10.32 | -11.94 | -7.97 | -9.92 | -13.56 | -8.78 | -8.51 | -6.46 | -8.96 | -16.91 | -13.70 | -11.57 |
Mitochondria | -1.99 | 1.90 | 0.28 | 2.22 | -2.06 | -4.06 | -0.87 | -2.22 | 2.69 | -1.88 | -4.56 | -0.39 | -1.93 | 3.22 | -1.69 |
Mitotic Spindle | -4.20 | -1.09 | -3.24 | 1.23 | 0.06 | -3.46 | -5.23 | -3.26 | -0.16 | 1.47 | -5.57 | -5.26 | -4.67 | 0.94 | 1.33 |
None | 0.99 | 0.96 | 1.14 | 1.68 | 2.31 | 0.79 | 0.00 | -0.32 | -1.21 | -0.42 | 0.59 | 0.10 | 0.27 | -0.40 | 0.29 |
Nuclear Periphery | -1.37 | -9.38 | -5.93 | -5.68 | -0.56 | -0.83 | -12.30 | -2.48 | -1.07 | 11.50 | -0.32 | -12.73 | -5.84 | -5.48 | 9.78 |
Nuclear Periphery Foci | 2.16 | -0.69 | -7.40 | -15.58 | -9.74 | 2.41 | -14.36 | -15.08 | -15.87 | -3.28 | 2.01 | -14.96 | -21.06 | -22.00 | -7.89 |
Nucleolus | -1.75 | 0.72 | 0.01 | 1.43 | -0.48 | -2.64 | -9.10 | -2.62 | 2.22 | 4.20 | -4.47 | -9.53 | -1.89 | 2.86 | 1.07 |
Nucleus | 1.24 | -5.58 | 1.82 | 9.01 | 21.23 | -26.67 | -41.36 | -13.61 | 15.09 | 35.39 | -29.62 | -42.85 | -11.84 | 17.25 | 38.34 |
Peroxisomes | -5.03 | 3.25 | 2.38 | 8.34 | -3.53 | 0.21 | 7.02 | 10.58 | 6.77 | 2.14 | -1.55 | 7.65 | 11.15 | 10.67 | 2.20 |
Vacuole | -7.97 | -6.46 | -7.12 | 1.19 | 0.34 | -33.93* | -20.71 | -11.70 | 20.94 | 5.66 | -43.68* | -22.98 | -21.17 | 16.39 | -0.44 |
Vacuole Periphery | 0.01 | 1.75 | -0.32 | -0.55 | -3.25 | 2.73 | 7.93 | 4.82 | 2.37 | -2.94 | 3.09 | 8.16 | 3.51 | 0.98 | -3.94 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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