Standard name
Human Ortholog
Description Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.56 0.63 0.67 0.62 0.35 0.25 0.24 0.25 0.27 0.22 0.19 0.23 0.72 0.79 0.8 0.35 0.25 0.24 0.44 0.39 0.39 0.65 0.72 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0 0.12 0.28 0.25 0.58 0.65 0.66 0.77 0.81 0.85 0.8 0.06 0 0 0.08 0 0 0.05 0.05 0.12 0 0 0
Nucleus 0.13 0.18 0.11 0.1 0.31 0.37 0.34 0.29 0.26 0.2 0.2 0.21 0.15 0.09 0.06 0.32 0.45 0.4 0.21 0.2 0.16 0.12 0.07 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.3 0.18 0.12 0.25 0.11 0.11 0.07 0.05 0.08 0 0.06 0.11 0.18 0.18 0.28 0.31 0.35 0.19 0.24 0.24 0.13 0.08 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 0 0 1 1
Bud 1 0 0 0 0 4 2 10 3 9 3 3 0 0 0 0 0 0 3 8 1 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 3 3
Bud Site 0 0 0 0 0 0 5 5 5 2 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 147 235 138 100 74 58 86 92 72 78 43 75 102 212 213 158 93 68 145 173 97 45 141 134
Endoplasmic Reticulum 1 2 1 0 0 1 0 0 1 0 0 1 1 0 0 29 27 20 0 1 3 0 2 0
Endosome 9 1 1 1 7 1 1 0 0 0 0 0 0 7 3 33 19 20 5 8 0 0 2 0
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 2 1 1 0 0 0 0 0
Mitochondria 87 11 24 44 54 136 232 246 209 295 191 258 8 3 2 35 4 6 16 20 30 2 8 4
Nucleus 35 66 22 16 65 87 121 108 70 73 45 68 21 23 16 144 167 111 69 90 41 8 14 2
Nuclear Periphery 3 0 0 0 0 0 2 2 2 2 4 4 1 0 1 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 0 0 1 0 0 2 1 1 2 1 0 0 0 2 1 1 11 14 4 1 3 2
Vac/Vac Membrane 35 111 37 20 53 25 40 26 13 29 9 18 15 47 47 128 114 98 61 108 61 8 16 21
Unique Cell Count 263 374 207 160 213 236 358 373 270 362 224 321 141 268 266 450 370 278 330 445 252 70 198 179
Labelled Cell Count 319 427 223 181 254 312 491 493 376 489 300 432 148 292 282 531 427 325 330 445 252 70 198 179


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.2 3.8 3.9 4.0 3.5 3.4 3.4 3.2 2.9 2.9 2.9 4.6 4.4 4.3 5.9 6.7 6.1 4.0 4.4 4.9
Std Deviation (1e-4) 0.6 0.7 0.8 1.5 1.4 1.0 1.7 1.0 1.4 1.0 0.6 1.8 1.0 1.2 1.3 1.6 1.6 1.4 0.8 1.7 1.0
Intensity Change (Log2) – – – 0.01 0.07 -0.13 -0.17 -0.17 -0.25 -0.39 -0.42 -0.38 0.25 0.21 0.18 0.61 0.8 0.68 0.05 0.2 0.34

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.9 3.4 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0276 -0.576 -0.6796 -0.4106 -1.059 -0.5053 1.1094 1.7118 1.546 2.5466 1.7383 1.7901 2.8158 2.7764 2.7099 3.8443 3.5387 3.097
Actin 0.0039 0.0015 0.0027 0.0001 0.0044 0.0019 0.0058 0.0023 0.0023 0.0011 0.0152 0.0009 0.0134 0.0003 0.0093 0.0034 0.001 0.0033
Bud 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.001 0.0004 0.0005 0.0004 0.0004 0.0002 0.001 0.0041 0.0031 0.0003 0.0002 0.0004
Bud Neck 0.0014 0.0003 0.0003 0.0001 0.0007 0.0032 0.0021 0.0005 0.0011 0.0056 0.0128 0.0015 0.0094 0.0004 0.0003 0.0015 0.0019 0.0072
Bud Periphery 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0021 0.0004 0.001 0.0005 0.001 0.0004 0.0023 0.0024 0.0032 0.0004 0.0004 0.0008
Bud Site 0.0033 0.0018 0.0007 0 0.0003 0.0003 0.0067 0.004 0.0007 0.0172 0.002 0.0004 0.0022 0.0027 0.0012 0.0006 0.0006 0.0002
Cell Periphery 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0005 0.0003 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.1608 0.0759 0.108 0.0403 0.1388 0.1192 0.0818 0.0998 0.0778 0.0635 0.0685 0.1021 0.0648 0.1621 0.083 0.0529 0.0947 0.1172
Cytoplasmic Foci 0.052 0.0135 0.0048 0.0025 0.0094 0.0153 0.0078 0.0074 0.0041 0.0067 0.0112 0.008 0.0079 0.0049 0.0073 0.0076 0.0029 0.0104
Eisosomes 0.0004 0.0001 0.0004 0.0001 0.0007 0.0003 0.0002 0.0001 0.0001 0.0002 0.0008 0.0001 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0025 0.0022 0.0019 0.0014 0.0034 0.0019 0.002 0.0014 0.0016 0.0007 0.0015 0.0009 0.0022 0.0014 0.0015 0.0019 0.0025 0.0015
Endosome 0.0046 0.0035 0.0018 0.0004 0.0041 0.0051 0.0041 0.0034 0.0016 0.0198 0.0051 0.0021 0.0043 0.0019 0.0038 0.012 0.0034 0.0035
Golgi 0.0021 0.001 0.0002 0 0.0004 0.0005 0.0012 0.0002 0.0002 0.0031 0.0015 0.0002 0.0015 0.0001 0.0011 0.0016 0.0002 0.0008
Lipid Particles 0.006 0.0074 0.0015 0.0002 0.0019 0.0041 0.0025 0.0004 0.0004 0.0122 0.0026 0.001 0.0052 0.0002 0.002 0.0042 0.0005 0.0023
Mitochondria 0.0003 0.0008 0.0069 0.0002 0.0007 0.0006 0.0011 0.0005 0.0007 0.0027 0.0015 0.0005 0.0038 0.0009 0.0013 0.0011 0.0013 0.0007
None 0.6487 0.8062 0.7161 0.8056 0.712 0.6915 0.7865 0.6805 0.7779 0.6867 0.7461 0.743 0.8183 0.7135 0.7855 0.7657 0.7139 0.7013
Nuclear Periphery 0.0028 0.0036 0.0035 0.0018 0.0085 0.0053 0.0062 0.0051 0.0041 0.0029 0.0035 0.0032 0.0069 0.0034 0.0039 0.0111 0.0065 0.0036
Nucleolus 0.0013 0.0027 0.0015 0.0007 0.0085 0.0032 0.0017 0.0029 0.0021 0.0109 0.0017 0.002 0.002 0.0018 0.0016 0.0027 0.0092 0.0015
Nucleus 0.0903 0.0611 0.1311 0.1433 0.0833 0.1286 0.0653 0.1535 0.0973 0.1233 0.1054 0.0988 0.0188 0.0814 0.0737 0.0958 0.1315 0.1182
Peroxisomes 0.0062 0.0018 0.0048 0.0001 0.0008 0.0009 0.0049 0.0005 0.001 0.0036 0.0018 0.0006 0.0224 0.0003 0.0035 0.0014 0.0003 0.0031
Punctate Nuclear 0.0108 0.0143 0.0123 0.0027 0.0199 0.0156 0.015 0.0349 0.0247 0.0286 0.016 0.0335 0.011 0.0162 0.0135 0.0331 0.0267 0.0227
Vacuole 0.0022 0.0015 0.0012 0.0004 0.0017 0.0016 0.0014 0.0015 0.0007 0.009 0.0007 0.0005 0.0015 0.0015 0.0007 0.0017 0.0016 0.0009
Vacuole Periphery 0.0001 0.0002 0.0002 0.0001 0.0003 0.0004 0.0003 0.0002 0.0002 0.0009 0.0003 0.0002 0.0005 0.0002 0.0003 0.0008 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5535 8.9207 3.7826 6.1477 4.6208 7.124 11.7579 12.8277 10.4411 9.9666
Translational Efficiency 0.796 0.7379 1.0945 0.7696 1.161 1.1828 0.7409 0.6348 0.78 0.8746

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1896 1287 299 2076 271 1188 1137 2311 2167 2475 1436 4387

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 626.22 644.47 888.75 845.67 729.72 680.02 847.12 842.01 639.16 661.53 855.79 843.74
Standard Deviation 66.62 83.84 89.23 115.73 66.75 91.18 99.68 116.11 74.92 89.23 99.05 115.95
Intensity Change Log 2 — 0.041444 0.505108 0.433425 — -0.101766 0.215223 0.206494 — -0.033856 0.356366 0.315748

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000026 0.000294 0.000719 0.001009 0.000071 0.000206 0.000332 0.000794 0.000032 0.000252 0.000413 0.000896
Bud Neck 0.006332 0.027326 0.003942 0.019658 0.003506 0.028819 0.002621 0.024344 0.005978 0.028042 0.002896 0.022127
Bud Site 0.001822 0.008779 0.003235 0.023985 0.001439 0.007454 0.001272 0.037137 0.001774 0.008143 0.001681 0.030913
Cell Periphery 0.000085 0.000181 0.000078 0.000361 0.000170 0.000216 0.000081 0.001245 0.000096 0.000198 0.000080 0.000827
Cytoplasm 0.455135 0.328715 0.027276 0.086016 0.271963 0.280170 0.039573 0.189719 0.432228 0.305413 0.037012 0.140645
Cytoplasmic Foci 0.070265 0.138615 0.001487 0.004328 0.122328 0.145847 0.000537 0.003469 0.076776 0.142087 0.000735 0.003875
Eisosomes 0.000048 0.000097 0.000044 0.000025 0.000114 0.000117 0.000023 0.000037 0.000056 0.000107 0.000028 0.000031
Endoplasmic Reticulum 0.000311 0.001613 0.009008 0.004812 0.000873 0.001226 0.005100 0.002393 0.000381 0.001428 0.005914 0.003538
Endosome 0.002032 0.011158 0.000779 0.003142 0.004229 0.019277 0.000458 0.003223 0.002307 0.015055 0.000525 0.003185
Golgi 0.000510 0.002887 0.000047 0.002448 0.000466 0.005819 0.000038 0.002314 0.000505 0.004294 0.000039 0.002377
Lipid Particles 0.003585 0.009471 0.000951 0.000659 0.006636 0.014766 0.000383 0.000477 0.003967 0.012013 0.000502 0.000563
Mitochondria 0.002346 0.016851 0.000865 0.004639 0.000881 0.020945 0.000902 0.004721 0.002163 0.018816 0.000895 0.004682
Mitotic Spindle 0.000364 0.007149 0.000300 0.040351 0.000039 0.015883 0.001819 0.026838 0.000323 0.011341 0.001503 0.033233
None 0.009130 0.013835 0.001744 0.007719 0.003933 0.013006 0.005497 0.016049 0.008480 0.013437 0.004716 0.012107
Nuclear Periphery 0.000615 0.001041 0.001199 0.002989 0.000779 0.001528 0.001123 0.001786 0.000636 0.001275 0.001139 0.002355
Nuclear Periphery Foci 0.000168 0.000542 0.000359 0.000983 0.000481 0.000668 0.000301 0.000416 0.000207 0.000603 0.000313 0.000684
Nucleolus 0.001438 0.005635 0.001881 0.000871 0.002822 0.008968 0.001111 0.001762 0.001611 0.007235 0.001271 0.001341
Nucleus 0.431697 0.370798 0.928523 0.776627 0.556244 0.378500 0.929335 0.654882 0.447272 0.374495 0.929166 0.712494
Peroxisomes 0.001492 0.012956 0.000193 0.001330 0.002649 0.014247 0.000088 0.000330 0.001637 0.013576 0.000110 0.000803
Vacuole 0.012374 0.039564 0.017212 0.016943 0.020065 0.039563 0.009330 0.025694 0.013336 0.039564 0.010971 0.021553
Vacuole Periphery 0.000224 0.002494 0.000157 0.001103 0.000312 0.002775 0.000074 0.002370 0.000235 0.002629 0.000092 0.001771

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.38 -3.36 -7.78 -4.86 -0.33 -4.44 -13.03 -11.66 -8.30 -4.11 -10.48 -9.53 -12.78 -8.17 -3.23
Bud Neck -9.01 1.08 -10.63 2.46 -11.45 -9.66 0.11 -12.54 2.34 -14.99 -12.18 2.71 -13.90 3.35 -20.69
Bud Site -7.25 -0.68 -7.63 -3.59 -5.57 -5.62 -0.01 -13.49 -9.20 -13.69 -7.92 -0.03 -14.35 -9.32 -14.82
Cell Periphery -6.98 -0.23 -5.27 -1.90 -4.89 -1.43 5.28 -5.97 -5.48 -7.22 -8.05 2.25 -8.16 -5.84 -8.43
Cytoplasm 13.13 52.91 56.74 30.92 -8.19 -0.42 17.00 9.05 15.99 -21.53 16.42 62.80 50.68 32.44 -21.24
Cytoplasmic Foci -11.88 24.48 24.44 26.91 -0.71 -2.13 12.62 12.46 26.73 -4.23 -14.33 27.92 27.34 37.93 -4.53
Eisosomes -10.96 -1.84 6.03 15.15 4.74 0.07 8.04 7.60 13.40 -2.19 -12.05 6.82 8.66 19.77 1.02
Endoplasmic Reticulum -12.33 -7.38 -24.93 -17.94 3.01 -1.24 -15.17 -10.08 -10.86 9.15 -12.35 -17.97 -30.27 -20.49 6.50
Endosome -10.13 2.26 0.75 10.41 -1.45 -8.77 4.61 3.04 11.67 -4.13 -14.47 6.10 1.78 15.40 -4.98
Golgi -4.93 3.13 -1.23 3.23 -2.61 -6.07 2.74 -1.48 5.04 -2.78 -7.60 3.57 -1.81 5.97 -3.82
Lipid Particles -7.32 5.02 7.41 12.26 2.54 -4.95 5.84 5.86 11.45 0.39 -10.20 9.07 9.37 16.13 1.24
Mitochondria -7.33 2.09 -2.04 6.83 -6.94 -9.27 -0.41 -8.01 7.85 -7.27 -10.83 1.95 -2.63 10.41 -9.73
Mitotic Spindle -4.19 -0.91 -10.55 -7.04 -9.55 -5.90 -2.26 -11.26 -2.73 -9.52 -7.13 -2.11 -15.24 -6.91 -13.17
None -2.58 9.06 1.89 3.81 -9.21 -5.75 -0.96 -8.21 0.61 -7.78 -3.86 5.14 -1.64 3.04 -8.51
Nuclear Periphery -4.57 -8.96 -19.29 -16.02 -13.10 -4.78 -6.91 -9.93 -5.40 -5.43 -7.72 -12.83 -21.79 -15.09 -12.88
Nuclear Periphery Foci -7.05 -2.38 -3.73 -1.86 -1.38 -1.44 0.57 1.31 3.24 1.01 -8.23 -4.27 -4.04 -0.37 -1.55
Nucleolus -6.47 -1.17 -0.28 6.37 0.99 -3.92 1.27 0.61 7.68 -3.92 -9.73 1.72 -0.46 9.93 -3.12
Nucleus 5.69 -41.49 -42.31 -40.58 10.44 8.69 -19.64 -8.75 -31.05 26.43 8.44 -64.63 -41.43 -50.02 27.74
Peroxisomes -9.99 4.31 2.35 10.87 -1.65 -8.46 5.68 5.42 10.81 -3.83 -13.46 5.76 4.34 15.37 -2.47
Vacuole -12.78 -3.62 -9.71 2.85 -0.32 -5.57 0.49 -3.65 2.59 -4.40 -15.73 -4.23 -12.73 3.87 -4.23
Vacuole Periphery -7.08 1.56 -2.31 5.52 -2.69 -9.51 4.51 -3.41 4.34 -4.40 -11.59 6.06 -4.37 6.75 -5.30
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (13%), nucleus (11%), mixed (55%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mad3

Mad3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mad3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available