Standard name | |
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Human Ortholog | |
Description | Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 1 | 1 |
Bud | 1 | 0 | 0 | 0 | 0 | 4 | 2 | 10 | 3 | 9 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 8 | 1 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 3 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 5 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 147 | 235 | 138 | 100 | 74 | 58 | 86 | 92 | 72 | 78 | 43 | 75 | 102 | 212 | 213 | 158 | 93 | 68 | 145 | 173 | 97 | 45 | 141 | 134 |
Endoplasmic Reticulum | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 29 | 27 | 20 | 0 | 1 | 3 | 0 | 2 | 0 |
Endosome | 9 | 1 | 1 | 1 | 7 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 3 | 33 | 19 | 20 | 5 | 8 | 0 | 0 | 2 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 87 | 11 | 24 | 44 | 54 | 136 | 232 | 246 | 209 | 295 | 191 | 258 | 8 | 3 | 2 | 35 | 4 | 6 | 16 | 20 | 30 | 2 | 8 | 4 |
Nucleus | 35 | 66 | 22 | 16 | 65 | 87 | 121 | 108 | 70 | 73 | 45 | 68 | 21 | 23 | 16 | 144 | 167 | 111 | 69 | 90 | 41 | 8 | 14 | 2 |
Nuclear Periphery | 3 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 2 | 4 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 11 | 14 | 4 | 1 | 3 | 2 |
Vac/Vac Membrane | 35 | 111 | 37 | 20 | 53 | 25 | 40 | 26 | 13 | 29 | 9 | 18 | 15 | 47 | 47 | 128 | 114 | 98 | 61 | 108 | 61 | 8 | 16 | 21 |
Unique Cell Count | 263 | 374 | 207 | 160 | 213 | 236 | 358 | 373 | 270 | 362 | 224 | 321 | 141 | 268 | 266 | 450 | 370 | 278 | 330 | 445 | 252 | 70 | 198 | 179 |
Labelled Cell Count | 319 | 427 | 223 | 181 | 254 | 312 | 491 | 493 | 376 | 489 | 300 | 432 | 148 | 292 | 282 | 531 | 427 | 325 | 330 | 445 | 252 | 70 | 198 | 179 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.4 | 4.2 | 3.8 | 3.9 | 4.0 | 3.5 | 3.4 | 3.4 | 3.2 | 2.9 | 2.9 | 2.9 | 4.6 | 4.4 | 4.3 | 5.9 | 6.7 | 6.1 | 4.0 | 4.4 | 4.9 |
Std Deviation (1e-4) | 0.6 | 0.7 | 0.8 | 1.5 | 1.4 | 1.0 | 1.7 | 1.0 | 1.4 | 1.0 | 0.6 | 1.8 | 1.0 | 1.2 | 1.3 | 1.6 | 1.6 | 1.4 | 0.8 | 1.7 | 1.0 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.07 | -0.13 | -0.17 | -0.17 | -0.25 | -0.39 | -0.42 | -0.38 | 0.25 | 0.21 | 0.18 | 0.61 | 0.8 | 0.68 | 0.05 | 0.2 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.9 | 3.4 | 4.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.0276 | -0.576 | -0.6796 | -0.4106 | -1.059 | -0.5053 | 1.1094 | 1.7118 | 1.546 | 2.5466 | 1.7383 | 1.7901 | 2.8158 | 2.7764 | 2.7099 | 3.8443 | 3.5387 | 3.097 |
Actin | 0.0039 | 0.0015 | 0.0027 | 0.0001 | 0.0044 | 0.0019 | 0.0058 | 0.0023 | 0.0023 | 0.0011 | 0.0152 | 0.0009 | 0.0134 | 0.0003 | 0.0093 | 0.0034 | 0.001 | 0.0033 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.001 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0002 | 0.001 | 0.0041 | 0.0031 | 0.0003 | 0.0002 | 0.0004 |
Bud Neck | 0.0014 | 0.0003 | 0.0003 | 0.0001 | 0.0007 | 0.0032 | 0.0021 | 0.0005 | 0.0011 | 0.0056 | 0.0128 | 0.0015 | 0.0094 | 0.0004 | 0.0003 | 0.0015 | 0.0019 | 0.0072 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0021 | 0.0004 | 0.001 | 0.0005 | 0.001 | 0.0004 | 0.0023 | 0.0024 | 0.0032 | 0.0004 | 0.0004 | 0.0008 |
Bud Site | 0.0033 | 0.0018 | 0.0007 | 0 | 0.0003 | 0.0003 | 0.0067 | 0.004 | 0.0007 | 0.0172 | 0.002 | 0.0004 | 0.0022 | 0.0027 | 0.0012 | 0.0006 | 0.0006 | 0.0002 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.1608 | 0.0759 | 0.108 | 0.0403 | 0.1388 | 0.1192 | 0.0818 | 0.0998 | 0.0778 | 0.0635 | 0.0685 | 0.1021 | 0.0648 | 0.1621 | 0.083 | 0.0529 | 0.0947 | 0.1172 |
Cytoplasmic Foci | 0.052 | 0.0135 | 0.0048 | 0.0025 | 0.0094 | 0.0153 | 0.0078 | 0.0074 | 0.0041 | 0.0067 | 0.0112 | 0.008 | 0.0079 | 0.0049 | 0.0073 | 0.0076 | 0.0029 | 0.0104 |
Eisosomes | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0025 | 0.0022 | 0.0019 | 0.0014 | 0.0034 | 0.0019 | 0.002 | 0.0014 | 0.0016 | 0.0007 | 0.0015 | 0.0009 | 0.0022 | 0.0014 | 0.0015 | 0.0019 | 0.0025 | 0.0015 |
Endosome | 0.0046 | 0.0035 | 0.0018 | 0.0004 | 0.0041 | 0.0051 | 0.0041 | 0.0034 | 0.0016 | 0.0198 | 0.0051 | 0.0021 | 0.0043 | 0.0019 | 0.0038 | 0.012 | 0.0034 | 0.0035 |
Golgi | 0.0021 | 0.001 | 0.0002 | 0 | 0.0004 | 0.0005 | 0.0012 | 0.0002 | 0.0002 | 0.0031 | 0.0015 | 0.0002 | 0.0015 | 0.0001 | 0.0011 | 0.0016 | 0.0002 | 0.0008 |
Lipid Particles | 0.006 | 0.0074 | 0.0015 | 0.0002 | 0.0019 | 0.0041 | 0.0025 | 0.0004 | 0.0004 | 0.0122 | 0.0026 | 0.001 | 0.0052 | 0.0002 | 0.002 | 0.0042 | 0.0005 | 0.0023 |
Mitochondria | 0.0003 | 0.0008 | 0.0069 | 0.0002 | 0.0007 | 0.0006 | 0.0011 | 0.0005 | 0.0007 | 0.0027 | 0.0015 | 0.0005 | 0.0038 | 0.0009 | 0.0013 | 0.0011 | 0.0013 | 0.0007 |
None | 0.6487 | 0.8062 | 0.7161 | 0.8056 | 0.712 | 0.6915 | 0.7865 | 0.6805 | 0.7779 | 0.6867 | 0.7461 | 0.743 | 0.8183 | 0.7135 | 0.7855 | 0.7657 | 0.7139 | 0.7013 |
Nuclear Periphery | 0.0028 | 0.0036 | 0.0035 | 0.0018 | 0.0085 | 0.0053 | 0.0062 | 0.0051 | 0.0041 | 0.0029 | 0.0035 | 0.0032 | 0.0069 | 0.0034 | 0.0039 | 0.0111 | 0.0065 | 0.0036 |
Nucleolus | 0.0013 | 0.0027 | 0.0015 | 0.0007 | 0.0085 | 0.0032 | 0.0017 | 0.0029 | 0.0021 | 0.0109 | 0.0017 | 0.002 | 0.002 | 0.0018 | 0.0016 | 0.0027 | 0.0092 | 0.0015 |
Nucleus | 0.0903 | 0.0611 | 0.1311 | 0.1433 | 0.0833 | 0.1286 | 0.0653 | 0.1535 | 0.0973 | 0.1233 | 0.1054 | 0.0988 | 0.0188 | 0.0814 | 0.0737 | 0.0958 | 0.1315 | 0.1182 |
Peroxisomes | 0.0062 | 0.0018 | 0.0048 | 0.0001 | 0.0008 | 0.0009 | 0.0049 | 0.0005 | 0.001 | 0.0036 | 0.0018 | 0.0006 | 0.0224 | 0.0003 | 0.0035 | 0.0014 | 0.0003 | 0.0031 |
Punctate Nuclear | 0.0108 | 0.0143 | 0.0123 | 0.0027 | 0.0199 | 0.0156 | 0.015 | 0.0349 | 0.0247 | 0.0286 | 0.016 | 0.0335 | 0.011 | 0.0162 | 0.0135 | 0.0331 | 0.0267 | 0.0227 |
Vacuole | 0.0022 | 0.0015 | 0.0012 | 0.0004 | 0.0017 | 0.0016 | 0.0014 | 0.0015 | 0.0007 | 0.009 | 0.0007 | 0.0005 | 0.0015 | 0.0015 | 0.0007 | 0.0017 | 0.0016 | 0.0009 |
Vacuole Periphery | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0009 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0008 | 0.0005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.5535 | 8.9207 | 3.7826 | 6.1477 | 4.6208 | 7.124 | 11.7579 | 12.8277 | 10.4411 | 9.9666 |
Translational Efficiency | 0.796 | 0.7379 | 1.0945 | 0.7696 | 1.161 | 1.1828 | 0.7409 | 0.6348 | 0.78 | 0.8746 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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