Standard name
Human Ortholog
Description Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; required for vacuolar protein sorting; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p; human ortholog is implicated in progressive cerebello-cerebral atrophy type 2 (PCCA2)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0.09 0.05 0 0 0.07 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.06 – 0.06 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 0 0 – 0 0 0 0 0.06 0 0.25 0.17 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.17 0.05 0.24 0.14 0.26 0.06 0.05 0 – 0 0 0 0.19 0.31 0.29 0.46 0.47 0.43 0.23 0.27 0.23 0.2 0.17 0.16
Golgi 0.11 0.06 0 0 0 0 0 0 – 0 0 0 0.35 0.26 0.4 0 0.14 0.3 0.35 0.4 0.31 0.22 0.29 0.39
Mitochondria 0.74 0.9 0.63 0.72 0.65 0.83 0.83 0.84 – 0.88 0.9 0.87 0.38 0.34 0.17 0.12 0.2 0 0 0 0.05 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.05 0 0 0 0.06 0.09 0.08 – 0.07 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.15 0.07 0.4 0.22 0.24 0.12 0.17 0.15 – 0.1 0.1 0.14 0.11 0.25 0 0.12 0.1 0.13 0.31 0.21 0.35 0.46 0.45 0.36
SpindlePole 0.07 0 0.12 0.12 0.12 0.08 0.09 0.1 – 0.08 0.09 0.09 0.15 0.08 0.18 0.32 0.27 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0.08 0.07 0 0.05 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 14 2 1 0 2 1 5 – 0 1 0 26 8 8 1 4 2 5 10 9 6 18 18
Bud 6 4 13 10 14 17 36 51 – 49 53 56 5 1 4 0 0 0 0 0 0 1 0 0
Bud Neck 0 0 1 0 0 0 0 1 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 2 1 0 1 2 14 21 25 – 26 32 25 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 0 1 1 1 2 – 7 3 4 1 1 1 2 0 0 0 0 0 0 0 0
Cytoplasm 4 4 20 15 19 15 16 16 – 17 12 2 9 10 9 23 10 2 1 0 0 0 0 1
Endoplasmic Reticulum 1 6 4 0 1 2 0 4 – 0 0 0 10 1 6 3 2 1 1 0 1 2 1 2
Endosome 47 16 113 46 99 33 40 27 – 8 6 17 58 50 59 42 28 10 72 88 113 67 81 92
Golgi 30 20 8 6 9 0 0 0 – 0 0 0 107 42 80 4 8 7 110 130 151 74 140 219
Mitochondria 200 289 301 234 244 453 613 715 – 724 573 629 114 55 34 11 12 1 10 12 25 17 20 17
Nucleus 1 0 3 1 1 1 2 8 – 6 2 2 0 1 1 6 3 0 1 0 0 0 0 0
Nuclear Periphery 0 4 1 1 1 3 4 4 – 6 3 3 4 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 5 16 9 12 6 33 63 72 – 55 35 39 2 3 4 4 1 0 0 1 0 0 1 0
Peroxisomes 41 22 193 71 92 66 126 129 – 79 62 102 33 41 7 11 6 3 97 68 173 154 220 204
SpindlePole 20 11 58 39 47 43 64 82 – 69 56 67 45 13 37 29 16 3 6 8 13 8 3 3
Vac/Vac Membrane 7 4 4 0 8 3 10 8 – 3 4 5 8 13 15 2 3 2 1 1 2 1 1 0
Unique Cell Count 272 321 479 324 378 547 736 850 822 638 723 303 161 201 92 59 23 312 326 497 338 493 565
Labelled Cell Count 368 412 732 437 544 686 997 1149 1050 842 952 422 239 265 138 93 31 312 326 497 338 493 565


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 6.5 6.2 5.7 5.6 4.7 4.6 4.8 – 4.2 4.2 4.4 7.6 7.8 7.9 7.4 8.5 9.9 7.8 8.2 8.2
Std Deviation (1e-4) 0.7 0.9 1.3 1.5 1.2 1.1 1.2 1.0 – 1.1 1.0 1.0 0.9 1.1 0.9 1.7 2.0 1.8 1.4 1.3 1.4
Intensity Change (Log2) – – – -0.12 -0.14 -0.4 -0.42 -0.37 – -0.56 -0.54 -0.48 0.29 0.33 0.35 0.26 0.45 0.68 0.34 0.4 0.41

WT3RAP60RAP140RAP220RAP300RAP380RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 6.0 0 0 0 0 0
Bud 0.3 0.8 0 0 0 – 0 0 0 -1.0 0 -0.6 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0.6 -1.3 -2.0 -2.5 – -2.2 -2.3 -4.9 -0.9 1.1 0.2 6.9 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 2.5 0 0 0 0 0
Endosome -3.3 0.9 0 0 0 – 0 0 0 -1.5 1.9 1.6 4.4 3.9 2.2
Golgi 0.2 0.7 0 0 0 – 0 0 0 12.9 10.0 13.5 0 0 0
Mitochondria 2.8 0.5 0 0 0 – 0 0 0 -6.9 -6.3 -10.9 -9.0 -6.2 -5.6
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 1.6 -0.3 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes -5.4 -4.9 0 0 0 – 0 0 0 -8.8 -3.4 -9.6 -5.2 -4.5 -2.6
SpindlePole 0 0.1 0 0 0 – 0 0 0 1.1 -1.4 2.2 4.7 3.2 0
Vacuole 0 1.6 0 0.8 0 – 0 0 0 0 0 4.8 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0776 3.6173 3.328 3.0208 2.8036 3.098 5.0385 4.8269 4.4131 3.998 5.2051 4.608 3.8772 5.1581 4.5858 4.9932 4.2495 4.7255
Actin 0.0369 0.0108 0.0164 0.0064 0.0019 0.0163 0.0795 0.0156 0.0399 0.0044 0.0253 0.0184 0.0057 0.0029 0.0085 0.0026 0.0009 0.0086
Bud 0.0005 0.0004 0.0001 0.0001 0.0001 0.0002 0.0008 0.0008 0.0009 0 0.0013 0.0002 0.0001 0.0002 0.0002 0.0001 0 0.0009
Bud Neck 0.0017 0.0008 0.0009 0.0016 0.0005 0.0006 0.0037 0.0008 0.0012 0.0001 0.0006 0.0007 0.0002 0.0003 0.0002 0.0003 0.0002 0.0004
Bud Periphery 0.0005 0.0003 0.0001 0.0002 0.0001 0.0002 0.001 0.0005 0.0008 0 0.0019 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0003
Bud Site 0.003 0.0055 0.0006 0.0013 0.0022 0.0007 0.016 0.0137 0.0057 0.0001 0.0024 0.0005 0.0004 0.0013 0.0003 0.0004 0.0001 0.0002
Cell Periphery 0.0002 0.0001 0 0.0002 0 0.0001 0.0005 0.0001 0.0001 0 0.0001 0.0001 0 0 0 0 0 0.0001
Cytoplasm 0.0072 0.0034 0.0015 0.0042 0.002 0.0008 0.0064 0.011 0.0023 0.0007 0.0029 0.0021 0.0006 0.0029 0.0014 0.0005 0.0004 0.0042
Cytoplasmic Foci 0.1499 0.2328 0.1981 0.1316 0.2346 0.1355 0.1474 0.2283 0.1806 0.1192 0.113 0.1683 0.1381 0.2275 0.1647 0.16 0.2179 0.1355
Eisosomes 0.0003 0.0001 0.0001 0.0008 0 0 0.0008 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0043 0.0004 0.0009 0.0005 0.0001 0.0003 0.0048 0.0014 0.0009 0.0001 0.001 0.0005 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001
Endosome 0.1652 0.2275 0.1692 0.1828 0.1719 0.1336 0.186 0.1923 0.1596 0.2535 0.1409 0.1219 0.1545 0.1894 0.1633 0.1001 0.1293 0.0977
Golgi 0.1713 0.2591 0.2561 0.317 0.3977 0.3752 0.1026 0.2015 0.2163 0.1387 0.1203 0.2333 0.174 0.274 0.2584 0.1972 0.2707 0.2218
Lipid Particles 0.1311 0.0548 0.0911 0.1392 0.0642 0.1226 0.1203 0.0714 0.1121 0.0386 0.2056 0.0847 0.1371 0.0439 0.0777 0.0834 0.0581 0.0847
Mitochondria 0.013 0.0215 0.0208 0.0076 0.0089 0.0252 0.0192 0.0126 0.0136 0.0043 0.0073 0.0186 0.0134 0.0066 0.0165 0.0301 0.0064 0.0134
None 0.0053 0.0002 0.0009 0.026 0.0002 0.0001 0.0085 0.0029 0.0005 0.0001 0.0022 0.0039 0.0003 0.0085 0.001 0.0001 0.0002 0.0002
Nuclear Periphery 0.0251 0.0002 0.0013 0.0012 0.0001 0.0002 0.0279 0.0006 0.0007 0 0.0248 0.0006 0.0001 0.0002 0.0001 0.0001 0 0.0001
Nucleolus 0.0005 0.0003 0.0002 0.0053 0.0001 0.0014 0.001 0.0003 0.0001 0 0.0023 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001
Nucleus 0.0055 0.0002 0.0004 0.0053 0.0001 0.0002 0.0043 0.0003 0.0005 0 0.0576 0.0001 0.0001 0.0001 0 0 0 0.0003
Peroxisomes 0.2685 0.173 0.238 0.1559 0.1132 0.182 0.2501 0.2278 0.2608 0.4376 0.2638 0.3432 0.3647 0.2384 0.3053 0.4227 0.3107 0.4298
Punctate Nuclear 0.0047 0.0023 0.0015 0.0112 0.001 0.0028 0.0147 0.0065 0.0015 0.0003 0.0233 0.0012 0.0088 0.001 0.0004 0.0006 0.003 0.0004
Vacuole 0.0029 0.0049 0.0008 0.0008 0.0006 0.0011 0.0023 0.0099 0.0012 0.0013 0.0018 0.0006 0.0011 0.0015 0.0009 0.0008 0.001 0.0008
Vacuole Periphery 0.0024 0.0014 0.0008 0.0008 0.0006 0.0009 0.0021 0.0014 0.0007 0.0008 0.0014 0.0006 0.0006 0.0007 0.0006 0.0007 0.0007 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.6969 23.5708 18.6763 21.9823 20.28 24.1194 25.4481 27.2631 22.3429 25.9217
Translational Efficiency 0.4983 0.5709 0.5804 0.4851 0.5298 0.4541 0.4794 0.4577 0.602 0.5423

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
267 792 1387 1016 2213 1028 1906 1740 2480 1820 3293 2756

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 958.26 863.01 1030.67 983.25 787.25 833.32 967.78 1011.83 805.66 846.24 994.27 1001.29
Standard Deviation 139.48 119.93 126.45 130.57 93.73 102.40 111.22 127.61 112.89 111.35 121.90 129.45
Intensity Change Log 2 — -0.151040 0.105093 0.037141 — 0.082049 0.297857 0.362073 — -0.041232 0.195233 0.192798

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.011015 0.003508 0.018290 0.024235 0.002350 0.002864 0.003781 0.015203 0.003283 0.003144 0.009892 0.018533
Bud Neck 0.002133 0.000550 0.000547 0.001090 0.000228 0.000207 0.000599 0.000512 0.000433 0.000356 0.000577 0.000725
Bud Site 0.019223 0.000837 0.002040 0.009096 0.000759 0.000593 0.001661 0.005545 0.002747 0.000699 0.001821 0.006854
Cell Periphery 0.000321 0.000039 0.000044 0.000059 0.000050 0.000033 0.000026 0.000049 0.000079 0.000035 0.000034 0.000052
Cytoplasm 0.005639 0.000986 0.000997 0.002772 0.001331 0.000524 0.000935 0.003128 0.001795 0.000725 0.000961 0.002997
Cytoplasmic Foci 0.295760 0.361907 0.482468 0.387593 0.216732 0.278747 0.469968 0.356264 0.225240 0.314935 0.475233 0.367813
Eisosomes 0.000110 0.000047 0.000057 0.000058 0.000068 0.000051 0.000030 0.000053 0.000073 0.000049 0.000041 0.000055
Endoplasmic Reticulum 0.001796 0.000220 0.000444 0.000230 0.000084 0.000249 0.000180 0.000182 0.000269 0.000236 0.000291 0.000200
Endosome 0.292041 0.179572 0.176584 0.215837 0.187853 0.217606 0.215167 0.226659 0.199070 0.201055 0.198916 0.222670
Golgi 0.166495 0.311314 0.202029 0.199412 0.451004 0.332230 0.156623 0.237450 0.420373 0.323128 0.175748 0.223427
Lipid Particles 0.012846 0.025907 0.041909 0.009224 0.011671 0.023198 0.020506 0.003257 0.011797 0.024377 0.029521 0.005457
Mitochondria 0.007879 0.014266 0.003170 0.004682 0.062683 0.025903 0.003891 0.012480 0.056783 0.020839 0.003587 0.009605
Mitotic Spindle 0.046666 0.010717 0.035844 0.046127 0.004552 0.003046 0.055191 0.024712 0.009086 0.006384 0.047042 0.032607
None 0.000049 0.000021 0.000013 0.000022 0.000117 0.000016 0.000010 0.000013 0.000110 0.000018 0.000011 0.000016
Nuclear Periphery 0.000253 0.000134 0.000180 0.000080 0.000014 0.000015 0.000098 0.000043 0.000040 0.000066 0.000133 0.000057
Nuclear Periphery Foci 0.001643 0.000447 0.001473 0.000801 0.000075 0.000094 0.001317 0.000260 0.000244 0.000248 0.001383 0.000460
Nucleolus 0.000128 0.000176 0.000036 0.000057 0.000355 0.000054 0.000115 0.000016 0.000330 0.000107 0.000082 0.000032
Nucleus 0.000249 0.000323 0.000040 0.000066 0.000156 0.000023 0.000037 0.000019 0.000166 0.000154 0.000038 0.000036
Peroxisomes 0.111716 0.085984 0.028111 0.089094 0.057553 0.112517 0.064891 0.107289 0.063384 0.100970 0.049399 0.100581
Vacuole 0.017324 0.001649 0.004583 0.008040 0.001165 0.000995 0.004234 0.005699 0.002904 0.001280 0.004381 0.006562
Vacuole Periphery 0.006713 0.001398 0.001141 0.001423 0.001201 0.001037 0.000739 0.001167 0.001794 0.001194 0.000908 0.001261

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.49 -2.05 -3.12 -6.45 -1.56 -0.86 -2.26 -8.26 -7.79 -7.15 0.21 -6.24 -9.76 -9.89 -4.86
Bud Neck 3.77 3.83 2.36 -3.25 -3.65 0.50 -3.74 -5.31 -5.39 0.83 1.15 -1.74 -3.78 -4.83 -1.83
Bud Site 4.12 3.86 2.14 -5.47 -4.70 0.92 -4.77 -7.11 -7.34 -5.75 3.99 1.88 -4.73 -8.72 -7.16
Cell Periphery 2.05 2.01 1.89 -3.12 -2.36 2.66 4.69 0.08 -2.22 -3.71 2.74 2.86 1.59 -3.45 -4.16
Cytoplasm 2.13 2.05 1.37 -3.70 -3.57 2.23 1.05 -3.18 -5.78 -4.81 2.54 1.85 -2.31 -6.57 -5.82
Cytoplasmic Foci -5.02 -14.44 -6.90 -2.64 10.86 -10.29 -42.29 -24.54 -10.99 16.21 -17.17 -50.81 -28.81 -9.13 19.68
Eisosomes 1.36 1.13 1.10 -2.80 -0.24 4.00 11.80 4.07 -0.45 -8.83 3.89 5.31 2.98 -2.05 -5.53
Endoplasmic Reticulum 2.94 2.51 2.93 -0.19 5.15 -0.86 -3.76 -1.99 0.33 -0.05 0.25 -0.43 0.98 0.30 2.59
Endosome 7.67 8.07 5.19 -4.50 -5.32 -4.52 -4.97 -6.84 -1.27 -1.90 -0.39 0.02 -4.72 -4.05 -5.06
Golgi -12.64 -3.51 -3.14 12.97 0.43 14.74 50.60 32.92 12.23 -14.91 14.46 44.48 33.79 17.23 -10.94
Lipid Particles -3.12 -7.51 1.34 4.71 10.31 -3.68 -4.35 7.05 6.77 9.72 -5.17 -9.33 5.58 8.38 14.30
Mitochondria -2.29 2.34 1.50 4.23 -1.36 9.19 18.91 14.84 4.58 -5.72 11.04 19.03 16.10 5.73 -5.82
Mitotic Spindle 4.16 1.23 0.05 -7.00 -1.89 1.83 -14.57 -9.22 -9.92 7.60 1.84 -14.38 -9.75 -11.01 4.44
None 1.09 1.33 1.03 -0.30 -1.83 1.24 1.30 1.28 1.10 -1.07 1.27 1.35 1.29 0.52 -2.07
Nuclear Periphery 1.17 0.93 2.29 0.71 3.80 -0.11 -8.15 -3.49 -3.28 4.16 -0.83 -7.01 -1.58 0.28 5.72
Nuclear Periphery Foci 2.35 0.17 1.54 -1.21 2.04 -0.49 -4.47 -2.65 -2.29 3.75 -0.00 -5.81 -1.96 -1.69 4.39
Nucleolus -0.47 1.82 1.32 1.32 -0.45 1.10 0.82 1.27 0.86 1.49 0.92 0.99 1.25 1.66 1.32
Nucleus -0.14 1.45 1.34 1.11 -0.52 1.14 1.03 1.19 0.33 2.14 0.12 1.21 1.27 1.17 0.46
Peroxisomes 2.00 7.10 1.78 -0.40 -10.68 -8.52 -1.73 -9.34 0.71 -7.56 -7.54 3.96 -8.37 0.07 -12.55
Vacuole 5.79 4.68 3.16 -6.33 -3.43 0.98 -8.28 -6.13 -6.25 -1.79 4.65 -3.62 -5.54 -8.74 -3.48
Vacuole Periphery 2.73 2.97 2.78 -0.08 -1.06 0.54 3.72 0.20 -0.43 -3.36 1.67 3.82 2.03 -0.25 -2.72
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; required for vacuolar protein sorting; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p; human ortholog is implicated in progressive cerebello-cerebral atrophy type 2 (PCCA2)
Localization
Cell Percentages Golgi (5%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vps53

Vps53


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps53-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available