Standard name
Human Ortholog
Description Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.84 0.98 0.96 0.89 0.91 0.8 0.83 – 0.73 0.66 0.58 0.6 0.88 0.85 0.82 0.45 0.41 0.41 0.82 0.86 0.82 0.83 0.75 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.12 0.12 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.19 0.09 – 0.25 0.29 0.42 0.42 0 0 0 0 0 0 0 0 0.07 0.05 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07
Vac/Vac Membrane 0.19 0 0 0.09 0.08 0 0.14 – 0.12 0.12 0.11 0.08 0.12 0.16 0.24 0.54 0.61 0.57 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 0 0 0 0 1 0 0 1 0 0 3 0 0 0 6 7
Bud 0 0 0 0 0 2 0 – 4 9 2 4 0 1 0 0 0 0 0 0 0 0 17 7
Bud Neck 0 0 0 0 0 1 2 – 3 1 0 1 0 0 0 0 0 0 1 1 1 0 3 9
Bud Site 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 1 1 1 2 1 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 269 159 163 192 277 163 393 – 280 316 209 246 240 384 410 101 57 35 288 162 162 171 383 311
Endoplasmic Reticulum 2 3 5 1 5 8 0 – 0 1 0 0 1 0 0 6 2 4 6 0 3 3 7 8
Endosome 10 0 1 5 4 0 4 – 4 7 5 7 9 20 16 14 17 10 16 3 4 5 17 17
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0
Mitochondria 2 1 0 4 4 39 43 – 97 139 152 172 2 3 2 3 2 2 4 4 13 9 16 9
Nucleus 1 0 1 1 2 6 5 – 13 10 13 11 2 0 2 7 2 0 3 2 1 1 3 4
Nuclear Periphery 0 0 0 0 0 1 0 – 1 1 0 1 0 1 0 0 0 0 3 1 1 0 5 5
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 1 3 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 1 0 0 6 – 5 1 2 3 0 1 2 0 0 0 7 2 3 3 27 27
Vac/Vac Membrane 62 0 2 20 25 4 69 – 45 58 38 31 33 71 122 122 85 49 8 5 1 2 8 8
Unique Cell Count 321 162 170 216 306 203 476 384 481 361 410 274 453 501 226 139 86 353 189 198 206 510 425
Labelled Cell Count 346 164 173 225 319 226 522 452 544 421 477 289 487 556 254 165 100 353 189 198 206 510 425


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.9 5.8 5.4 6.0 3.9 4.9 – 4.3 4.3 4.2 4.2 5.9 5.7 6.2 7.4 8.1 7.7 5.2 5.5 5.7
Std Deviation (1e-4) 0.6 0.6 1.0 1.3 1.5 1.0 1.1 – 1.0 0.9 1.0 0.7 1.2 1.5 1.4 1.2 1.3 1.2 1.6 1.4 1.8
Intensity Change (Log2) – – – -0.09 0.04 -0.56 -0.25 – -0.44 -0.42 -0.46 -0.47 0.02 -0.02 0.08 0.35 0.48 0.41 -0.17 -0.09 -0.03

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 11.2 11.7 10.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.616 -0.0594 -0.2491 -0.8036 -0.9523 -0.3047 0.2879 0.7297 1.0558 0.2371 0.8135 0.8857 3.2993 3.2003 2.8372 3.0027 2.24 2.8988
Actin 0.0164 0.0002 0.0163 0.0015 0.0002 0.0138 0.0265 0.0073 0.0171 0.041 0.0558 0.003 0.0763 0.0002 0.0169 0.0004 0.0431 0.0101
Bud 0.0003 0.0002 0.0106 0.0287 0 0.0003 0.0003 0.0001 0.0058 0.0007 0.0084 0.0001 0.0015 0 0.0016 0.0001 0.0012 0
Bud Neck 0.0001 0.0003 0.0003 0.0003 0.0002 0.0016 0.0004 0.0001 0.0005 0.0005 0.0011 0.0006 0.009 0.0001 0.0021 0.0001 0.0003 0.0008
Bud Periphery 0.0006 0.0001 0.0024 0.0063 0 0.0003 0.0004 0 0.0013 0.0021 0.0079 0.0001 0.0019 0 0.0018 0.0001 0.0017 0.0001
Bud Site 0.0008 0.0022 0.0055 0.0032 0.0001 0.0005 0.0009 0.0004 0.0026 0.0014 0.0028 0.0001 0.0191 0.0003 0.0067 0.0001 0.0021 0.0001
Cell Periphery 0.0001 0.0001 0.0002 0.0004 0 0.0001 0.0001 0.0001 0.0002 0.0003 0.0003 0 0.0007 0.0001 0.0003 0 0.0001 0
Cytoplasm 0.3061 0.4744 0.3154 0.3779 0.5699 0.4804 0.4794 0.6606 0.6495 0.656 0.4929 0.7855 0.3045 0.6074 0.5416 0.4018 0.6429 0.6454
Cytoplasmic Foci 0.0104 0.0139 0.0177 0.0151 0.0053 0.0191 0.0298 0.0053 0.0061 0.0041 0.1242 0.007 0.0322 0.0046 0.0055 0.0023 0.0074 0.0072
Eisosomes 0.0003 0 0.0002 0.0002 0 0.0002 0.0003 0.0001 0.0002 0.0014 0.001 0 0.0006 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0053 0.0072 0.0071 0.0095 0.0037 0.0081 0.0135 0.0048 0.006 0.0082 0.0028 0.0124 0.0065 0.004 0.0061 0.0011 0.0023 0.0028
Endosome 0.0061 0.0041 0.0038 0.0104 0.0024 0.0075 0.0154 0.002 0.0058 0.0184 0.0735 0.0057 0.0235 0.0016 0.006 0.0005 0.0031 0.0016
Golgi 0.0015 0.0002 0.0012 0.0005 0.0001 0.0023 0.0035 0.0004 0.0054 0.0032 0.0243 0.0037 0.0096 0.0001 0.0011 0 0.0036 0.0006
Lipid Particles 0.0031 0.0007 0.0057 0.0046 0.0001 0.0016 0.014 0.0012 0.0023 0.0008 0.0103 0.0117 0.013 0.0002 0.0019 0.0001 0.0041 0.0019
Mitochondria 0.0029 0.0003 0.0017 0.0015 0.0002 0.0017 0.0028 0.0001 0.0042 0.0208 0.0813 0.0015 0.0036 0.0001 0.0006 0.0001 0.0017 0.0003
None 0.6214 0.4721 0.5748 0.4829 0.3784 0.4245 0.3746 0.3048 0.2767 0.1894 0.0664 0.1526 0.4353 0.3763 0.3929 0.5596 0.2723 0.3059
Nuclear Periphery 0.0115 0.0042 0.0048 0.0136 0.0032 0.0063 0.0098 0.0031 0.0038 0.0172 0.0024 0.0038 0.0102 0.0011 0.0024 0.0016 0.0014 0.0025
Nucleolus 0.0002 0.0003 0.0005 0.0024 0.0001 0.0005 0.002 0.0001 0.0002 0.0003 0.0004 0.0001 0.0009 0 0.0005 0.0001 0.0001 0.0001
Nucleus 0.0046 0.012 0.0064 0.0255 0.0335 0.0215 0.0088 0.0044 0.0069 0.0163 0.0035 0.0075 0.0055 0.0025 0.0057 0.0302 0.0027 0.0101
Peroxisomes 0.003 0.0001 0.0085 0.0005 0 0.0005 0.0064 0.0022 0.0021 0.0005 0.0278 0.0006 0.0305 0 0.0011 0.0001 0.0072 0.0069
Punctate Nuclear 0.0023 0.0058 0.0155 0.0118 0.0013 0.0077 0.0086 0.0023 0.0021 0.0013 0.0046 0.0026 0.0126 0.0006 0.0034 0.0013 0.0016 0.003
Vacuole 0.002 0.0015 0.0013 0.0025 0.0009 0.0012 0.0018 0.0008 0.001 0.0087 0.0053 0.001 0.002 0.0007 0.0013 0.0002 0.0008 0.0004
Vacuole Periphery 0.0011 0.0002 0.0003 0.0009 0.0001 0.0004 0.0007 0.0001 0.0003 0.0072 0.0028 0.0005 0.001 0.0001 0.0002 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9361 5.007 7.4308 16.1025 9.6827 20.5258 10.547 13.0981 14.8632 15.7823
Translational Efficiency 2.0245 4.6612 3.015 1.5148 2.0291 1.1027 2.3367 1.5556 1.6001 1.5984

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2144 1433 1382 158 1943 2013 1887 1520 4087 3446 3269 1678

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 729.14 832.78 930.51 938.65 705.10 786.25 873.37 971.53 717.71 805.60 897.53 968.43
Standard Deviation 77.20 231.38 104.36 135.97 82.43 92.73 104.74 141.67 80.62 166.77 108.32 141.47
Intensity Change Log 2 — 0.191740 0.351826 0.364391 — 0.157160 0.308765 0.462431 — 0.174843 0.330817 0.413422

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000072 0.001491 0.000432 0.000501 0.000066 0.000157 0.000161 0.001021 0.000069 0.000712 0.000276 0.000972
Bud Neck 0.008389 0.012602 0.003317 0.009923 0.004987 0.015246 0.001680 0.009553 0.006772 0.014146 0.002372 0.009588
Bud Site 0.002032 0.051519 0.002898 0.008835 0.001645 0.010756 0.003189 0.024117 0.001848 0.027707 0.003066 0.022678
Cell Periphery 0.000082 0.012554 0.000091 0.000168 0.000081 0.000248 0.000078 0.000154 0.000081 0.005365 0.000084 0.000156
Cytoplasm 0.473736 0.468559 0.342929 0.343114 0.580007 0.475056 0.536082 0.393167 0.524259 0.472354 0.454425 0.388454
Cytoplasmic Foci 0.197688 0.182503 0.005297 0.010693 0.137165 0.128636 0.008147 0.022518 0.168915 0.151036 0.006942 0.021405
Eisosomes 0.000102 0.000202 0.000023 0.000054 0.000055 0.000092 0.000019 0.000033 0.000080 0.000138 0.000021 0.000035
Endoplasmic Reticulum 0.004047 0.007318 0.004810 0.011306 0.002251 0.002227 0.003101 0.007149 0.003193 0.004344 0.003824 0.007541
Endosome 0.007457 0.013962 0.001950 0.008630 0.004989 0.013025 0.001392 0.010801 0.006284 0.013415 0.001628 0.010597
Golgi 0.001640 0.001816 0.000081 0.000747 0.000876 0.001463 0.000121 0.004081 0.001276 0.001610 0.000104 0.003767
Lipid Particles 0.022202 0.013320 0.001150 0.004113 0.010086 0.009916 0.000976 0.002637 0.016442 0.011331 0.001049 0.002776
Mitochondria 0.004397 0.002370 0.000872 0.016833 0.001311 0.003987 0.000422 0.004920 0.002930 0.003315 0.000612 0.006042
Mitotic Spindle 0.002354 0.002136 0.004514 0.064979 0.002409 0.003758 0.007118 0.124398 0.002380 0.003083 0.006017 0.118804
None 0.003515 0.006262 0.005255 0.008215 0.006893 0.009875 0.006771 0.009285 0.005121 0.008372 0.006130 0.009185
Nuclear Periphery 0.003004 0.002530 0.003589 0.010471 0.001491 0.002082 0.003142 0.015050 0.002284 0.002268 0.003331 0.014618
Nuclear Periphery Foci 0.002243 0.005772 0.003219 0.072482 0.002584 0.001022 0.003847 0.027074 0.002405 0.002997 0.003581 0.031349
Nucleolus 0.001467 0.002187 0.000616 0.003189 0.001770 0.003139 0.000309 0.002275 0.001611 0.002743 0.000439 0.002361
Nucleus 0.230426 0.162725 0.585073 0.291345 0.219065 0.256699 0.381570 0.265931 0.225025 0.217620 0.467603 0.268324
Peroxisomes 0.003731 0.003874 0.000276 0.000529 0.001394 0.004200 0.000160 0.002657 0.002620 0.004065 0.000209 0.002457
Vacuole 0.030716 0.044956 0.033456 0.132423 0.020443 0.056661 0.041641 0.070457 0.025832 0.051793 0.038181 0.076291
Vacuole Periphery 0.000701 0.001344 0.000150 0.001449 0.000432 0.001755 0.000074 0.002720 0.000573 0.001584 0.000106 0.002600

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.11 -4.02 -6.94 1.12 -2.94 -3.25 -9.53 -8.57 -7.18 -6.37 -3.37 -6.19 -9.59 -0.37 -5.12
Bud Neck -2.74 4.53 -2.80 -1.77 -3.82 -7.66 2.89 -7.53 1.54 -12.44 -7.34 5.80 -7.16 0.27 -11.94
Bud Site -10.53 -1.85 -3.51 6.23 -3.14 -6.42 -1.95 -7.21 -1.16 -6.09 -12.03 -2.58 -7.75 5.51 -6.72
Cell Periphery -5.17 -3.60 -5.46 5.13 -4.20 -5.23 -0.00 -5.79 3.62 -5.68 -5.24 -2.33 -7.25 5.19 -5.75
Cytoplasm 0.56 21.10 13.31 12.61 4.34 12.87 15.40 33.45 20.22 17.92 8.45 22.46 32.24 23.75 13.02
Cytoplasmic Foci 2.41 44.72 43.26 33.35 -2.83 1.65 34.83 32.10 30.69 -4.02 4.36 55.80 50.69 43.08 -5.36
Eisosomes -2.33 16.79 0.85 2.48 -7.37 -7.71 11.78 3.19 9.78 -9.86 -3.20 20.52 10.26 4.81 -11.24
Endoplasmic Reticulum -4.36 -6.58 -6.67 -4.46 -4.71 0.18 -8.07 -16.01 -16.12 -10.15 -3.07 -8.91 -14.72 -10.83 -9.00
Endosome -5.56 10.35 -0.73 3.88 -5.81 -7.69 9.19 -2.60 5.51 -8.73 -9.07 14.03 -0.99 7.26 -9.19
Golgi -0.74 9.56 5.64 6.11 -3.00 -2.02 5.50 -0.89 0.57 -3.10 -1.70 10.83 0.35 1.25 -3.22
Lipid Particles 6.08 18.07 14.60 9.07 -5.43 0.19 11.68 9.17 8.21 -10.90 5.50 21.34 18.46 13.07 -12.00
Mitochondria 3.84 7.14 -0.04 -0.63 -1.05 -5.29 5.49 -3.76 2.35 -6.49 -0.97 8.43 0.26 0.99 -4.82
Mitotic Spindle 0.08 -2.53 -4.83 -4.84 -4.56 -1.13 -3.31 -17.57 -17.37 -16.46 -0.97 -4.19 -18.27 -18.16 -17.31
None -2.69 -2.57 -3.16 -1.75 -2.53 -2.24 0.66 -2.11 0.79 -3.70 -3.78 -1.44 -5.49 -0.68 -5.23
Nuclear Periphery 1.16 -6.23 -4.60 -4.79 -3.67 -1.67 -11.67 -15.49 -14.66 -12.76 0.09 -11.64 -15.44 -15.28 -13.20
Nuclear Periphery Foci -4.65 -3.55 -4.52 -4.15 -4.27 3.25 -3.74 -11.06 -12.09 -9.66 -1.28 -5.31 -12.15 -11.90 -10.81
Nucleolus -1.89 3.50 -3.04 -2.68 -3.31 -2.28 3.04 -4.73 -2.33 -9.78 -3.20 4.39 -7.06 -4.21 -9.98
Nucleus 10.44 -44.45 -4.03 -6.75 10.76 -5.62 -27.77 -10.89 -5.65 12.95 1.50 -47.90 -11.57 -11.93 23.38
Peroxisomes -0.27 10.54 9.12 8.35 -3.48 -4.78 8.15 1.66 5.36 -4.82 -3.53 12.96 6.67 7.69 -4.83
Vacuole -6.26 -9.21 -9.35 -8.61 -7.70 -14.19 -18.17 -24.05 -16.48 -11.99 -14.42 -19.34 -24.90 -19.53 -15.27
Vacuole Periphery -4.48 7.58 0.64 2.87 -1.46 -5.60 8.27 -1.78 2.47 -3.17 -6.68 10.91 -1.21 2.61 -3.18
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
Localization
Cell Percentages cytoplasm (24%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mad2

Mad2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mad2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available