Standard name
Human Ortholog
Description Subunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.47 0.48 0.53 0.49 0.6 0.66 0.64 0.52 0.57 0.45 0.52 0.4 0.56 0.53 0.32 0.2 0.22 0.16 0.34 0.26 0.31 0.37 0.35
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.41 0 0.12 0.37 0.19 0.27 0.26 0.52 0.39 0.54 0.55 0 0 0 0.08 0 0.05 0.07 0 0.05 0 0 0.05
Nucleus – 0.17 0.3 0.31 0.29 0.22 0.16 0.11 0.08 0.12 0.14 0.08 0.46 0.31 0.29 0.46 0.55 0.58 0.36 0.27 0.37 0.31 0.24 0.18
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05
Vac/Vac Membrane – 0.07 0.2 0.11 0 0.08 0 0.1 0 0 0 0.07 0.2 0.17 0.23 0.18 0.25 0.25 0.3 0.28 0.19 0.23 0.24 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
Bud – 1 0 0 2 0 3 1 3 4 3 5 0 1 0 0 0 0 1 0 2 1 4 5
Bud Neck – 0 0 0 3 6 5 8 2 2 2 4 0 2 1 0 0 0 0 0 0 1 4 6
Bud Site – 0 0 0 1 0 0 1 2 1 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 40 87 92 42 184 209 213 172 179 157 194 73 165 125 30 28 24 6 46 64 61 103 90
Endoplasmic Reticulum – 0 1 0 0 0 0 0 0 0 0 0 1 3 1 6 8 7 0 0 0 0 2 3
Endosome – 0 3 0 0 2 0 1 0 0 2 0 3 2 4 3 1 1 0 1 1 2 1 3
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 1 0 0 0
Mitochondria – 35 7 21 32 57 86 85 174 123 189 207 3 2 2 8 4 6 2 3 12 7 6 13
Nucleus – 15 55 54 25 68 51 36 28 38 47 29 85 91 68 44 79 64 15 36 91 62 66 47
Nuclear Periphery – 0 0 0 0 0 0 0 1 2 0 3 0 0 0 0 1 0 0 0 2 0 0 1
Nucleolus – 0 0 1 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 1 2 2 2 2 4 5 0 0 2 0 0 1 1 5 11 7 11 12
Vac/Vac Membrane – 6 37 19 3 25 12 34 11 10 14 25 36 49 55 17 36 27 12 37 47 46 65 62
Unique Cell Count 86 182 172 86 306 316 332 332 315 348 374 183 294 238 95 143 110 42 138 247 200 275 262
Labelled Cell Count 97 191 187 108 343 368 382 397 361 423 477 201 315 259 109 158 131 42 138 247 200 275 262


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 3.1 5.1 4.8 3.9 4.2 4.1 4.2 3.7 3.8 3.6 3.5 5.2 5.0 4.9 5.1 5.8 6.5 5.2 5.5 6.0
Std Deviation (1e-4) – 0.4 1.1 1.6 1.1 1.3 1.3 1.1 1.7 1.5 1.3 0.9 1.0 0.9 1.0 1.4 1.5 1.9 1.2 1.3 1.7
Intensity Change (Log2) – – – -0.09 -0.39 -0.28 -0.33 -0.27 -0.47 -0.43 -0.5 -0.53 0.03 -0.03 -0.07 -0.01 0.18 0.35 0.03 0.11 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -0.5 1.0 0.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1344 -0.7519 -0.9655 -1.2412 -1.0212 -0.8509 4.0692 4.1637 3.8614 4.1922 3.435 4.2368 0.8558 1.2718 0.9652 0.9073 1.2766 0.9883
Actin 0.0089 0.0001 0.0079 0.0004 0.0005 0.0007 0.0256 0.0006 0.0011 0.0002 0.0003 0.0007 0.0127 0.0004 0.0015 0.0004 0.0028 0.0051
Bud 0.0004 0.0003 0.0008 0 0.0047 0.0002 0.0006 0.0001 0.0002 0 0.0001 0.0001 0.0004 0.0011 0.0001 0.0001 0.0001 0.0002
Bud Neck 0.001 0.0004 0.0009 0.0005 0.0009 0.0025 0.0053 0.0004 0.0005 0.0003 0.0011 0.0022 0.0088 0.0007 0.0021 0.0009 0.0006 0.0046
Bud Periphery 0.0003 0.0001 0.0016 0.0001 0.0023 0.0001 0.0009 0.0001 0.0002 0 0.0001 0.0001 0.0009 0.0023 0.0001 0.0001 0.0001 0.0004
Bud Site 0.0008 0.0007 0.0067 0.0001 0.0003 0.0002 0.0116 0.0007 0.0091 0.0001 0.0017 0.0001 0.0034 0.0074 0.0005 0.0003 0.0004 0.0003
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0003 0 0.0001 0 0.0001 0 0.0003 0.0005 0.0001 0 0 0
Cytoplasm 0.1423 0.1844 0.0778 0.0643 0.1013 0.1 0.1123 0.1443 0.145 0.1452 0.1247 0.108 0.18 0.1824 0.1118 0.1408 0.0701 0.129
Cytoplasmic Foci 0.0093 0.0026 0.0071 0.0036 0.0162 0.0054 0.009 0.008 0.0051 0.0035 0.0049 0.0047 0.0146 0.0031 0.0053 0.0035 0.0179 0.0059
Eisosomes 0.0001 0 0.0001 0.0001 0 0 0.0003 0.0001 0.0001 0 0 0 0.0002 0 0.0001 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0044 0.0038 0.0029 0.001 0.0029 0.0015 0.0046 0.002 0.0018 0.0015 0.0029 0.0014 0.0049 0.005 0.0015 0.0014 0.0011 0.0013
Endosome 0.0097 0.0037 0.005 0.002 0.0053 0.0043 0.0167 0.001 0.0046 0.0125 0.0176 0.0038 0.0105 0.0026 0.0065 0.0014 0.0084 0.0024
Golgi 0.0018 0.0001 0.002 0.0001 0.0004 0.0002 0.0048 0.0002 0.0027 0.0001 0.0029 0.0002 0.0027 0.0002 0.002 0.0001 0.0031 0.0004
Lipid Particles 0.0055 0.0003 0.0033 0.0012 0.016 0.0007 0.0086 0.0003 0.0034 0.0002 0.0071 0.0005 0.004 0.0003 0.0037 0.0002 0.0077 0.0006
Mitochondria 0.0022 0.0005 0.0028 0.0041 0.0007 0.0004 0.0027 0.0005 0.0009 0.0005 0.0023 0.0005 0.0046 0.0014 0.0012 0.0009 0.0013 0.0006
None 0.1669 0.1575 0.2003 0.2815 0.3115 0.1549 0.2583 0.3746 0.2305 0.2495 0.2207 0.2775 0.3187 0.3408 0.2618 0.397 0.5055 0.2893
Nuclear Periphery 0.0232 0.0442 0.0177 0.0182 0.027 0.0147 0.0245 0.0082 0.0144 0.0119 0.0149 0.0075 0.0179 0.0165 0.0086 0.0117 0.0084 0.0073
Nucleolus 0.0034 0.0029 0.0042 0.0041 0.0038 0.0065 0.0059 0.0034 0.0029 0.0026 0.0051 0.0043 0.005 0.0029 0.0028 0.0043 0.0026 0.0041
Nucleus 0.5892 0.565 0.6107 0.5907 0.4056 0.6373 0.4664 0.4241 0.5423 0.5551 0.5314 0.5318 0.3691 0.4032 0.5564 0.4092 0.322 0.4982
Peroxisomes 0.004 0.0001 0.0069 0.0003 0.0007 0.0002 0.006 0.0041 0.001 0.0001 0.0011 0.001 0.0046 0.0001 0.0014 0.0002 0.0218 0.0029
Punctate Nuclear 0.0229 0.0308 0.0395 0.0262 0.0984 0.069 0.0298 0.026 0.0326 0.0145 0.0554 0.0545 0.0314 0.0234 0.0308 0.0262 0.0249 0.0455
Vacuole 0.0029 0.0022 0.0012 0.0007 0.0009 0.0009 0.0043 0.0012 0.0013 0.0017 0.0047 0.0011 0.0041 0.0049 0.0015 0.0009 0.0006 0.0016
Vacuole Periphery 0.0007 0.0006 0.0004 0.0007 0.0005 0.0003 0.0014 0.0002 0.0003 0.0006 0.0009 0.0002 0.001 0.0007 0.0003 0.0002 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.6534 23.5787 6.8042 10.7293 13.2992 16.1099 18.5425 12.515 13.0654 13.2439
Translational Efficiency 0.9968 0.5962 0.9985 0.5917 0.6814 0.9529 0.482 0.7051 0.5896 0.6248

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1721 254 115 1409 1790 2114 153 1546 3511 2368 268 2955

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 648.75 782.70 909.83 825.36 664.95 807.97 905.47 803.09 657.01 805.26 907.34 813.71
Standard Deviation 73.59 96.90 103.00 127.39 86.29 93.33 103.63 140.25 80.73 94.05 103.38 134.73
Intensity Change Log 2 — 0.270797 0.487934 0.347361 — 0.281056 0.445421 0.272316 — 0.275999 0.466572 0.309864

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000075 0.000385 0.000884 0.000468 0.000028 0.000122 0.000442 0.000327 0.000051 0.000150 0.000631 0.000394
Bud Neck 0.007129 0.013557 0.001538 0.010424 0.001701 0.009578 0.001470 0.007579 0.004362 0.010004 0.001499 0.008935
Bud Site 0.001611 0.007370 0.001117 0.016790 0.001094 0.002889 0.004105 0.017937 0.001347 0.003370 0.002823 0.017390
Cell Periphery 0.000133 0.000231 0.000193 0.000220 0.000098 0.000133 0.000115 0.000300 0.000115 0.000143 0.000149 0.000262
Cytoplasm 0.305987* 0.168414* 0.045383 0.232104* 0.308785* 0.214327* 0.041344 0.214123* 0.307414* 0.209402* 0.043077 0.222697*
Cytoplasmic Foci 0.085713 0.058092 0.003375 0.009519 0.027675 0.031058 0.001795 0.006181 0.056124 0.033958 0.002473 0.007773
Eisosomes 0.000070 0.000076 0.000104 0.000037 0.000025 0.000031 0.000052 0.000034 0.000047 0.000036 0.000074 0.000035
Endoplasmic Reticulum 0.000624 0.004097 0.013747 0.002884 0.000538 0.000858 0.007162 0.002144 0.000580 0.001205 0.009987 0.002497
Endosome 0.002159 0.005910 0.001830 0.007263 0.000804 0.002409 0.000927 0.002642 0.001468 0.002785 0.001315 0.004846
Golgi 0.001482 0.004013 0.000080 0.004495 0.000085 0.001056 0.000042 0.001129 0.000770 0.001373 0.000058 0.002734
Lipid Particles 0.005655 0.005318 0.002462 0.000871 0.001380 0.002243 0.001282 0.000809 0.003475 0.002573 0.001788 0.000838
Mitochondria 0.002912 0.005107 0.000576 0.004655 0.000426 0.004170 0.000624 0.001506 0.001645 0.004270 0.000603 0.003007
Mitotic Spindle 0.001079 0.002583 0.000161 0.060323 0.000038 0.004707 0.000966 0.018943 0.000549 0.004479 0.000620 0.038674
None 0.009158 0.007289 0.002693 0.009178 0.012565 0.006096 0.002773 0.013373 0.010895 0.006224 0.002739 0.011373
Nuclear Periphery 0.000735 0.001498 0.001614 0.003199 0.000605 0.001311 0.001231 0.001413 0.000669 0.001331 0.001395 0.002265
Nuclear Periphery Foci 0.000333 0.001074 0.001057 0.001555 0.000209 0.000276 0.000485 0.000734 0.000270 0.000362 0.000730 0.001126
Nucleolus 0.002650 0.005439 0.001958 0.002008 0.001221 0.001997 0.000751 0.001189 0.001921 0.002366 0.001269 0.001580
Nucleus 0.558991* 0.675318* 0.879249* 0.580762* 0.637839* 0.706005* 0.906670* 0.681355* 0.599190* 0.702713* 0.894904* 0.633390*
Peroxisomes 0.003168 0.009141 0.000394 0.000923 0.000376 0.002399 0.000217 0.000266 0.001744 0.003122 0.000293 0.000579
Vacuole 0.009918 0.023827 0.041404 0.045922 0.004387 0.007496 0.027425 0.025372 0.007098 0.009248 0.033423 0.035171
Vacuole Periphery 0.000417 0.001262 0.000183 0.006398 0.000121 0.000839 0.000122 0.002643 0.000266 0.000884 0.000148 0.004433

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.65 -5.75 -7.25 -0.40 3.90 -2.78 -7.20 -11.72 -5.65 3.84 -3.25 -8.68 -12.31 -6.10 5.30
Bud Neck -2.05 5.80 -2.51 1.23 -7.80 -7.44 -2.19 -8.36 1.20 -6.36 -5.40 3.76 -6.80 0.98 -9.97
Bud Site -3.14 -0.97 -6.25 -1.23 -5.97 -2.59 -1.52 -7.41 -6.27 -1.68 -3.38 -1.58 -9.65 -7.30 -2.97
Cell Periphery -2.31 -4.17 -1.12 1.94 3.42 -2.21 -3.43 -7.65 -4.74 -2.81 -1.89 -5.14 -7.08 -3.14 -0.12
Cytoplasm 9.76 21.14 14.15 -1.42 -11.40 11.78 23.84 12.97 2.14 -13.81 15.32 32.00 19.11 2.72 -17.87
Cytoplasmic Foci 3.36 22.37 23.69 7.77 -0.72 -1.52 14.70 13.00 13.44 -3.67 8.27 26.17 26.15 15.56 -2.72
Eisosomes -1.41 -4.71 4.81 3.84 6.11 -3.26 -6.52 -7.88 -4.38 4.83 3.95 -7.33 0.57 -4.32 7.60
Endoplasmic Reticulum -7.37 -10.59 -20.20 -0.67 7.94 -4.82 -8.09 -17.55 -14.09 6.32 -7.85 -12.90 -26.31 -17.76 9.77
Endosome -2.86 -1.05 -2.32 1.74 -1.24 -3.70 -2.80 -3.91 0.47 -1.22 -3.15 -2.25 -4.01 -0.06 -1.86
Golgi -0.92 3.66 -0.25 0.83 -2.40 -2.68 0.46 -3.00 0.68 -3.06 -1.27 3.69 -1.13 0.29 -3.34
Lipid Particles 0.14 3.74 9.06 5.17 5.16 -3.45 -1.60 4.16 5.62 3.36 2.26 2.89 9.69 7.00 5.98
Mitochondria -1.09 4.43 0.13 1.12 -3.08 -6.56 -1.57 -7.93 4.52 -3.69 -4.38 3.54 -1.19 3.33 -4.12
Mitotic Spindle -1.42 1.51 -9.06 -7.46 -9.33 -4.22 -1.56 -7.10 -4.54 -5.42 -3.93 -0.98 -11.42 -8.72 -10.56
None 1.24 6.25 1.54 -0.15 -5.64 5.07 7.84 1.04 -3.82 -6.67 5.34 10.05 1.66 -3.68 -8.70
Nuclear Periphery -3.51 -8.11 -10.20 -6.55 -5.35 -2.89 -7.61 -12.30 -2.30 -1.04 -3.06 -10.98 -13.57 -5.99 -5.34
Nuclear Periphery Foci -2.50 -3.68 -3.63 0.30 1.87 -0.98 -4.45 -4.34 -3.32 1.62 -1.29 -5.35 -5.36 -3.97 2.34
Nucleolus -2.09 1.49 2.67 3.07 1.34 -2.74 2.65 0.62 3.52 -2.70 -1.46 2.60 2.72 4.31 -0.06
Nucleus -5.34 -12.65 -6.82 1.66 8.93 -7.21 -10.38 -5.19 1.34 7.47 -13.19 -16.42 -8.56 4.55 11.66
Peroxisomes -1.77 7.60 6.79 2.55 0.00 -5.97 1.67 2.40 6.45 0.54 -2.86 7.83 7.11 5.79 0.07
Vacuole -3.98 -5.29 -18.48 -10.21 -0.40 -4.73 -5.67 -15.20 -13.53 2.51 -3.09 -7.72 -23.29 -21.55 1.35
Vacuole Periphery -1.81 2.21 -3.32 -1.61 -3.68 -2.14 -0.96 -3.19 -1.54 -3.13 -2.01 1.81 -4.59 -2.44 -4.83
Standard name
Human Ortholog
Description Subunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs
Localization
Cell Percentages nucleus (22%), cytoplasm (12%), mixed (60%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tad2

Tad2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tad2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available