Standard name | |
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Human Ortholog | |
Description | Subunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Bud | – | 1 | 0 | 0 | 2 | 0 | 3 | 1 | 3 | 4 | 3 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 4 | 5 |
Bud Neck | – | 0 | 0 | 0 | 3 | 6 | 5 | 8 | 2 | 2 | 2 | 4 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 6 |
Bud Site | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 40 | 87 | 92 | 42 | 184 | 209 | 213 | 172 | 179 | 157 | 194 | 73 | 165 | 125 | 30 | 28 | 24 | 6 | 46 | 64 | 61 | 103 | 90 |
Endoplasmic Reticulum | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 6 | 8 | 7 | 0 | 0 | 0 | 0 | 2 | 3 |
Endosome | – | 0 | 3 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 3 | 2 | 4 | 3 | 1 | 1 | 0 | 1 | 1 | 2 | 1 | 3 |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | – | 35 | 7 | 21 | 32 | 57 | 86 | 85 | 174 | 123 | 189 | 207 | 3 | 2 | 2 | 8 | 4 | 6 | 2 | 3 | 12 | 7 | 6 | 13 |
Nucleus | – | 15 | 55 | 54 | 25 | 68 | 51 | 36 | 28 | 38 | 47 | 29 | 85 | 91 | 68 | 44 | 79 | 64 | 15 | 36 | 91 | 62 | 66 | 47 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Nucleolus | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 2 | 4 | 5 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 5 | 11 | 7 | 11 | 12 |
Vac/Vac Membrane | – | 6 | 37 | 19 | 3 | 25 | 12 | 34 | 11 | 10 | 14 | 25 | 36 | 49 | 55 | 17 | 36 | 27 | 12 | 37 | 47 | 46 | 65 | 62 |
Unique Cell Count | 86 | 182 | 172 | 86 | 306 | 316 | 332 | 332 | 315 | 348 | 374 | 183 | 294 | 238 | 95 | 143 | 110 | 42 | 138 | 247 | 200 | 275 | 262 | |
Labelled Cell Count | 97 | 191 | 187 | 108 | 343 | 368 | 382 | 397 | 361 | 423 | 477 | 201 | 315 | 259 | 109 | 158 | 131 | 42 | 138 | 247 | 200 | 275 | 262 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 3.1 | 5.1 | 4.8 | 3.9 | 4.2 | 4.1 | 4.2 | 3.7 | 3.8 | 3.6 | 3.5 | 5.2 | 5.0 | 4.9 | 5.1 | 5.8 | 6.5 | 5.2 | 5.5 | 6.0 |
Std Deviation (1e-4) | – | 0.4 | 1.1 | 1.6 | 1.1 | 1.3 | 1.3 | 1.1 | 1.7 | 1.5 | 1.3 | 0.9 | 1.0 | 0.9 | 1.0 | 1.4 | 1.5 | 1.9 | 1.2 | 1.3 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.39 | -0.28 | -0.33 | -0.27 | -0.47 | -0.43 | -0.5 | -0.53 | 0.03 | -0.03 | -0.07 | -0.01 | 0.18 | 0.35 | 0.03 | 0.11 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | -0.5 | 1.0 | 0.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.1344 | -0.7519 | -0.9655 | -1.2412 | -1.0212 | -0.8509 | 4.0692 | 4.1637 | 3.8614 | 4.1922 | 3.435 | 4.2368 | 0.8558 | 1.2718 | 0.9652 | 0.9073 | 1.2766 | 0.9883 |
Actin | 0.0089 | 0.0001 | 0.0079 | 0.0004 | 0.0005 | 0.0007 | 0.0256 | 0.0006 | 0.0011 | 0.0002 | 0.0003 | 0.0007 | 0.0127 | 0.0004 | 0.0015 | 0.0004 | 0.0028 | 0.0051 |
Bud | 0.0004 | 0.0003 | 0.0008 | 0 | 0.0047 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Bud Neck | 0.001 | 0.0004 | 0.0009 | 0.0005 | 0.0009 | 0.0025 | 0.0053 | 0.0004 | 0.0005 | 0.0003 | 0.0011 | 0.0022 | 0.0088 | 0.0007 | 0.0021 | 0.0009 | 0.0006 | 0.0046 |
Bud Periphery | 0.0003 | 0.0001 | 0.0016 | 0.0001 | 0.0023 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0023 | 0.0001 | 0.0001 | 0.0001 | 0.0004 |
Bud Site | 0.0008 | 0.0007 | 0.0067 | 0.0001 | 0.0003 | 0.0002 | 0.0116 | 0.0007 | 0.0091 | 0.0001 | 0.0017 | 0.0001 | 0.0034 | 0.0074 | 0.0005 | 0.0003 | 0.0004 | 0.0003 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0005 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.1423 | 0.1844 | 0.0778 | 0.0643 | 0.1013 | 0.1 | 0.1123 | 0.1443 | 0.145 | 0.1452 | 0.1247 | 0.108 | 0.18 | 0.1824 | 0.1118 | 0.1408 | 0.0701 | 0.129 |
Cytoplasmic Foci | 0.0093 | 0.0026 | 0.0071 | 0.0036 | 0.0162 | 0.0054 | 0.009 | 0.008 | 0.0051 | 0.0035 | 0.0049 | 0.0047 | 0.0146 | 0.0031 | 0.0053 | 0.0035 | 0.0179 | 0.0059 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0044 | 0.0038 | 0.0029 | 0.001 | 0.0029 | 0.0015 | 0.0046 | 0.002 | 0.0018 | 0.0015 | 0.0029 | 0.0014 | 0.0049 | 0.005 | 0.0015 | 0.0014 | 0.0011 | 0.0013 |
Endosome | 0.0097 | 0.0037 | 0.005 | 0.002 | 0.0053 | 0.0043 | 0.0167 | 0.001 | 0.0046 | 0.0125 | 0.0176 | 0.0038 | 0.0105 | 0.0026 | 0.0065 | 0.0014 | 0.0084 | 0.0024 |
Golgi | 0.0018 | 0.0001 | 0.002 | 0.0001 | 0.0004 | 0.0002 | 0.0048 | 0.0002 | 0.0027 | 0.0001 | 0.0029 | 0.0002 | 0.0027 | 0.0002 | 0.002 | 0.0001 | 0.0031 | 0.0004 |
Lipid Particles | 0.0055 | 0.0003 | 0.0033 | 0.0012 | 0.016 | 0.0007 | 0.0086 | 0.0003 | 0.0034 | 0.0002 | 0.0071 | 0.0005 | 0.004 | 0.0003 | 0.0037 | 0.0002 | 0.0077 | 0.0006 |
Mitochondria | 0.0022 | 0.0005 | 0.0028 | 0.0041 | 0.0007 | 0.0004 | 0.0027 | 0.0005 | 0.0009 | 0.0005 | 0.0023 | 0.0005 | 0.0046 | 0.0014 | 0.0012 | 0.0009 | 0.0013 | 0.0006 |
None | 0.1669 | 0.1575 | 0.2003 | 0.2815 | 0.3115 | 0.1549 | 0.2583 | 0.3746 | 0.2305 | 0.2495 | 0.2207 | 0.2775 | 0.3187 | 0.3408 | 0.2618 | 0.397 | 0.5055 | 0.2893 |
Nuclear Periphery | 0.0232 | 0.0442 | 0.0177 | 0.0182 | 0.027 | 0.0147 | 0.0245 | 0.0082 | 0.0144 | 0.0119 | 0.0149 | 0.0075 | 0.0179 | 0.0165 | 0.0086 | 0.0117 | 0.0084 | 0.0073 |
Nucleolus | 0.0034 | 0.0029 | 0.0042 | 0.0041 | 0.0038 | 0.0065 | 0.0059 | 0.0034 | 0.0029 | 0.0026 | 0.0051 | 0.0043 | 0.005 | 0.0029 | 0.0028 | 0.0043 | 0.0026 | 0.0041 |
Nucleus | 0.5892 | 0.565 | 0.6107 | 0.5907 | 0.4056 | 0.6373 | 0.4664 | 0.4241 | 0.5423 | 0.5551 | 0.5314 | 0.5318 | 0.3691 | 0.4032 | 0.5564 | 0.4092 | 0.322 | 0.4982 |
Peroxisomes | 0.004 | 0.0001 | 0.0069 | 0.0003 | 0.0007 | 0.0002 | 0.006 | 0.0041 | 0.001 | 0.0001 | 0.0011 | 0.001 | 0.0046 | 0.0001 | 0.0014 | 0.0002 | 0.0218 | 0.0029 |
Punctate Nuclear | 0.0229 | 0.0308 | 0.0395 | 0.0262 | 0.0984 | 0.069 | 0.0298 | 0.026 | 0.0326 | 0.0145 | 0.0554 | 0.0545 | 0.0314 | 0.0234 | 0.0308 | 0.0262 | 0.0249 | 0.0455 |
Vacuole | 0.0029 | 0.0022 | 0.0012 | 0.0007 | 0.0009 | 0.0009 | 0.0043 | 0.0012 | 0.0013 | 0.0017 | 0.0047 | 0.0011 | 0.0041 | 0.0049 | 0.0015 | 0.0009 | 0.0006 | 0.0016 |
Vacuole Periphery | 0.0007 | 0.0006 | 0.0004 | 0.0007 | 0.0005 | 0.0003 | 0.0014 | 0.0002 | 0.0003 | 0.0006 | 0.0009 | 0.0002 | 0.001 | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0002 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.6534 | 23.5787 | 6.8042 | 10.7293 | 13.2992 | 16.1099 | 18.5425 | 12.515 | 13.0654 | 13.2439 |
Translational Efficiency | 0.9968 | 0.5962 | 0.9985 | 0.5917 | 0.6814 | 0.9529 | 0.482 | 0.7051 | 0.5896 | 0.6248 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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