Standard name
Human Ortholog
Description Microtubule-associated protein involved in microtubule organization; involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.06 – 0.05 0 0 0.05 0.08 0.06 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.95 0.97 0.97 0.99 0.98 0.91 0.87 0.83 0.8 – 0.72 0.79 0.99 0.99 0.98 0.93 0.94 0.95 0.88 0.92 0.93 0.94 0.92 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.21 0.19 0.24 0.34 – 0.37 0.34 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 1 1 0 3 – 4 1 0 0 1 0 0 0 6 3 0 1 1 8
Bud 0 1 1 2 1 4 6 5 5 – 2 6 5 2 22 0 0 0 1 2 0 1 2 7
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 1 0 1 3
Bud Site 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 8 11 8 5 8 15 22 14 23 – 18 15 29 36 59 38 21 19 0 0 0 0 0 1
Cytoplasm 209 486 425 263 324 444 485 479 327 – 251 321 639 667 716 570 291 312 207 461 411 462 538 601
Endoplasmic Reticulum 1 12 5 0 1 1 7 7 4 – 15 5 3 3 10 28 10 15 1 3 5 13 24 26
Endosome 3 0 0 0 0 0 0 0 1 – 0 0 4 1 1 2 1 2 1 2 4 0 0 9
Golgi 2 0 0 1 0 0 0 0 0 – 0 0 0 0 1 2 1 1 4 3 0 0 0 4
Mitochondria 0 3 5 13 12 103 108 140 138 – 128 137 3 0 4 4 3 9 4 3 8 3 2 5
Nucleus 0 3 0 0 0 0 4 9 0 – 2 2 1 1 3 11 11 5 0 1 0 0 0 0
Nuclear Periphery 0 1 2 1 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 1 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 4
Vac/Vac Membrane 5 4 0 2 1 2 3 8 5 – 1 6 0 1 1 33 10 7 1 9 2 5 6 9
Unique Cell Count 220 503 436 267 330 487 557 579 410 347 404 647 672 732 612 311 328 237 502 441 492 583 689
Labelled Cell Count 229 522 447 287 347 570 637 662 506 421 495 685 711 818 689 349 371 237 502 441 492 583 689


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 8.1 5.5 5.4 6.0 5.7 5.6 5.4 5.1 – 5.2 5.1 8.0 8.4 7.3 13.4 13.6 12.9 7.7 8.1 8.8
Std Deviation (1e-4) 1.6 1.2 0.9 0.8 1.2 0.9 1.0 1.0 1.0 – 1.0 1.1 1.3 1.2 1.1 2.4 2.6 2.4 1.3 1.3 1.4
Intensity Change (Log2) – – – -0.02 0.14 0.06 0.02 -0.03 -0.09 – -0.08 -0.09 0.54 0.61 0.42 1.29 1.31 1.24 0.49 0.57 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 3.3 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0.6 1.2 1.9 0.6 2.9 – 2.6 1.7 2.4 2.9 4.4 3.4 3.4 2.9
Cytoplasm 0.9 0.7 -4.1 -5.9 -7.5 -8.2 – -10.2 -8.3 1.6 2.4 0.4 -3.2 -2.6 -1.7
Endoplasmic Reticulum 0 0 0 0.2 0.1 0 – 2.8 0 0 0 0.3 3.1 2.0 2.9
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 3.0 2.3 9.4 9.0 10.4 12.6 – 13.2 12.7 0 0 0 0 0 1.6
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 4.9 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.5615 9.0207 8.6616 8.0884 8.0765 8.9247 2.0821 2.8058 2.4354 2.6255 1.6929 2.7189 6.4606 6.8076 6.8933 7.2804 6.6084 7.5304
Actin 0.0001 0.0004 0.0004 0.0004 0.0007 0.0004 0.0004 0.002 0.0012 0.0006 0.0008 0.0023 0.031 0.0082 0.0085 0.0031 0.016 0.0066
Bud 0.0008 0.0006 0.0007 0.0009 0.0003 0.0002 0.0002 0.0036 0.0018 0.0112 0.0005 0.0007 0.0054 0.0037 0.0294 0.016 0.0173 0.0037
Bud Neck 0.0007 0.0003 0.0005 0.0008 0.0006 0.0018 0.0008 0.0012 0.0007 0.001 0.0007 0.0129 0.0013 0.0005 0.0017 0.0018 0.0025 0.0077
Bud Periphery 0.0012 0.0008 0.001 0.0021 0.0002 0.0003 0.0002 0.0018 0.0012 0.0138 0.0003 0.0005 0.0033 0.0064 0.0128 0.0075 0.0265 0.0048
Bud Site 0.0006 0.0051 0.0021 0.0007 0.0003 0.0001 0.0005 0.0331 0.0109 0.0073 0.0004 0.0027 0.0077 0.0131 0.04 0.0197 0.0125 0.0022
Cell Periphery 0.0013 0.001 0.0008 0.0005 0.0002 0.0005 0.0004 0.0008 0.0005 0.0013 0.0002 0.0003 0.001 0.0005 0.001 0.0003 0.0009 0.0003
Cytoplasm 0.9215 0.9645 0.96 0.9215 0.9703 0.9607 0.8455 0.8518 0.876 0.7482 0.8762 0.8801 0.7396 0.8423 0.7609 0.7547 0.8047 0.8397
Cytoplasmic Foci 0.003 0.0037 0.0036 0.0069 0.0042 0.0042 0.0159 0.0172 0.0186 0.0337 0.0238 0.0147 0.0414 0.0173 0.022 0.0179 0.0251 0.0106
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0003 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0303 0.0059 0.0084 0.0102 0.0043 0.0104 0.0218 0.0067 0.008 0.015 0.0161 0.015 0.007 0.0037 0.0076 0.0043 0.0044 0.0029
Endosome 0.005 0.0039 0.0056 0.0169 0.0044 0.0045 0.0289 0.0182 0.0237 0.0763 0.042 0.0284 0.0251 0.007 0.0213 0.0256 0.0174 0.0102
Golgi 0.0003 0.0003 0.0003 0.001 0.0002 0.0006 0.0016 0.0015 0.0011 0.0012 0.0017 0.001 0.0085 0.0009 0.0047 0.0014 0.0028 0.0018
Lipid Particles 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0012 0.0006 0.0007 0.0004 0.0006 0.0003 0.0034 0.0003 0.0023 0.0008 0.0008 0.0002
Mitochondria 0.0004 0.0003 0.0003 0.001 0.0002 0.0005 0.0015 0.0006 0.0004 0.0005 0.0007 0.0005 0.0081 0.0005 0.0016 0.0007 0.0023 0.0005
None 0.0231 0.0058 0.007 0.0218 0.0058 0.0116 0.0636 0.042 0.038 0.0445 0.0191 0.0309 0.0966 0.0867 0.0704 0.1172 0.0474 0.1032
Nuclear Periphery 0.0013 0.0007 0.0009 0.0015 0.0006 0.0003 0.0026 0.0017 0.0014 0.0037 0.0028 0.0013 0.0019 0.0012 0.002 0.0042 0.0017 0.0007
Nucleolus 0 0 0 0.0001 0 0 0.0003 0.0008 0.0003 0.0006 0.0001 0.0001 0.0006 0.0002 0.0004 0.0006 0.0006 0.0001
Nucleus 0.0013 0.0013 0.0014 0.0017 0.0012 0.0007 0.0017 0.0053 0.0029 0.0049 0.0023 0.0019 0.0015 0.0021 0.0033 0.0058 0.0025 0.0018
Peroxisomes 0 0 0 0.0001 0.0001 0 0.0002 0.0002 0.0002 0.0003 0.0001 0.0002 0.0075 0.0006 0.002 0.0006 0.0062 0.0003
Punctate Nuclear 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0023 0.0017 0.0018 0.0007 0.0008 0.0025 0.0013 0.0024 0.0079 0.0009 0.0005
Vacuole 0.0081 0.0047 0.0061 0.0104 0.0056 0.0026 0.011 0.0079 0.0094 0.0298 0.0096 0.0045 0.0056 0.0033 0.0048 0.0084 0.0064 0.0017
Vacuole Periphery 0.0007 0.0004 0.0005 0.0013 0.0005 0.0003 0.0013 0.0008 0.0011 0.004 0.0015 0.0008 0.0008 0.0004 0.0008 0.0015 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.0137 19.9766 17.2651 21.4583 21.3041 17.8675 25.3668 28.7085 26.7899 30.4005
Translational Efficiency 0.5635 0.4284 0.5291 0.4515 0.469 0.6354 0.4749 0.4267 0.5132 0.4841

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
226 1575 155 386 1036 2072 575 1888 1262 3647 730 2274

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 788.65 866.17 1754.04 1132.51 863.73 914.09 1252.24 1037.00 850.28 893.40 1358.79 1053.21
Standard Deviation 116.49 151.12 268.24 177.38 137.44 135.01 171.62 142.58 136.99 144.16 283.89 153.31
Intensity Change Log 2 — 0.135265 1.153225 0.522067 — 0.081756 0.535859 0.263764 — 0.107542 0.863434 0.392824

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000220 0.000200 0.002901 0.000296 0.000299 0.000334 0.000530 0.000541 0.000285 0.000276 0.001034 0.000499
Bud Neck 0.012794 0.004020 0.007699 0.002280 0.004583 0.005257 0.002269 0.003448 0.006053 0.004723 0.003422 0.003250
Bud Site 0.022882 0.023074 0.036225 0.025563 0.020340 0.032091 0.027346 0.019622 0.020795 0.028197 0.029231 0.020631
Cell Periphery 0.000532 0.000165 0.003329 0.000104 0.000298 0.000142 0.000257 0.000117 0.000340 0.000152 0.000910 0.000115
Cytoplasm 0.596079* 0.722145* 0.676834* 0.551532* 0.669813* 0.677667* 0.653131* 0.671459* 0.656608* 0.696875* 0.658164* 0.651102*
Cytoplasmic Foci 0.184131* 0.127011 0.062253 0.075665 0.163896 0.167967 0.083204 0.042592 0.167519 0.150280 0.078756 0.048206
Eisosomes 0.000201 0.000023 0.000022 0.000016 0.000080 0.000043 0.000023 0.000017 0.000101 0.000034 0.000023 0.000016
Endoplasmic Reticulum 0.002509 0.001783 0.004407 0.000263 0.003896 0.001149 0.000788 0.000357 0.003647 0.001423 0.001557 0.000341
Endosome 0.031732 0.016420 0.012204 0.008807 0.016028 0.018662 0.007307 0.007281 0.018840 0.017694 0.008347 0.007540
Golgi 0.006145 0.005901 0.007049 0.002489 0.004916 0.011222 0.001987 0.001560 0.005136 0.008924 0.003062 0.001718
Lipid Particles 0.004523 0.001072 0.019437 0.001046 0.001932 0.001134 0.002439 0.000675 0.002396 0.001107 0.006048 0.000738
Mitochondria 0.001699 0.000752 0.000028 0.000163 0.000286 0.001735 0.000075 0.002533 0.000539 0.001311 0.000065 0.002131
Mitotic Spindle 0.000315 0.000641 0.003172 0.005052 0.000098 0.005564 0.000329 0.011051 0.000137 0.003438 0.000933 0.010033
None 0.000660 0.001747 0.000303 0.001810 0.002192 0.000898 0.000127 0.000539 0.001918 0.001265 0.000165 0.000755
Nuclear Periphery 0.000131 0.000050 0.000292 0.000107 0.000066 0.000040 0.000045 0.000106 0.000078 0.000044 0.000098 0.000106
Nuclear Periphery Foci 0.000970 0.000568 0.014814 0.001902 0.001601 0.000215 0.004034 0.000643 0.001488 0.000367 0.006323 0.000857
Nucleolus 0.000258 0.000264 0.000046 0.000159 0.000456 0.000153 0.000090 0.000202 0.000420 0.000201 0.000081 0.000195
Nucleus 0.007515 0.008173 0.015142 0.006104 0.012658 0.005533 0.005743 0.014972 0.011737 0.006673 0.007739 0.013467
Peroxisomes 0.001375 0.001538 0.000463 0.002045 0.001154 0.002122 0.000693 0.001281 0.001193 0.001870 0.000644 0.001411
Vacuole 0.121886 0.080448 0.131598 0.313790* 0.093325 0.064343 0.209232* 0.219208* 0.098440 0.071298 0.192748 0.235263*
Vacuole Periphery 0.003443 0.004007 0.001782 0.000806 0.002086 0.003730 0.000348 0.001794 0.002329 0.003850 0.000653 0.001626

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.42 -7.72 -0.36 -0.83 7.63 -0.59 -2.98 -1.45 -1.08 0.37 0.26 -7.42 -1.50 -1.63 4.76
Bud Neck 5.40 2.63 6.77 5.15 7.55 -1.63 7.17 4.71 6.52 -1.91 2.99 5.82 7.84 6.80 2.75
Bud Site -0.03 -3.07 0.81 0.93 3.33 -6.10 -2.37 2.90 8.27 4.32 -5.13 -3.59 2.96 7.63 5.54
Cell Periphery 4.96 -3.74 6.76 2.35 4.43 8.92 3.46 7.92 2.41 4.65 8.08 -3.19 10.09 2.81 4.89
Cytoplasm -7.95 -2.08 3.77 13.06 5.19 -0.94 2.98 0.36 1.41 -2.79 -5.31 1.88 1.75 7.72 -0.57
Cytoplasmic Foci 5.46 10.60 10.70 10.17 -0.53 -0.69 14.39 26.28 31.20 11.12 3.53 17.57 28.32 34.06 10.27
Eisosomes 6.79 6.78 7.06 3.56 3.09 6.13 10.74 12.51 9.35 5.51 10.01 12.03 13.38 10.00 6.25
Endoplasmic Reticulum 0.69 -0.87 2.46 2.98 3.54 2.28 2.75 3.08 4.11 2.36 2.18 2.34 3.45 4.51 4.15
Endosome 4.94 6.12 8.51 7.78 3.32 -1.98 9.17 10.36 12.06 1.48 0.97 10.64 13.12 14.41 2.95
Golgi 0.19 -0.09 3.06 4.61 4.57 -8.51 3.63 10.78 13.97 0.46 -6.47 3.26 9.90 14.72 1.82
Lipid Particles 2.12 -5.51 2.13 0.16 8.28 6.11 -1.46 9.63 4.75 7.15 4.15 -5.57 5.33 4.62 9.13
Mitochondria 0.87 1.54 1.49 3.84 -1.00 -3.96 4.69 -2.39 0.33 -2.83 -2.60 2.47 -1.56 0.06 -2.85
Mitotic Spindle -1.17 -4.41 -1.84 -1.57 0.68 -5.14 -2.62 -5.42 -1.42 -5.19 -5.39 -5.02 -5.67 -2.72 -4.79
None -1.76 1.43 -0.39 0.82 -0.91 2.21 3.54 2.91 2.68 -3.28 1.24 3.65 2.70 2.36 -2.55
Nuclear Periphery 0.99 -1.90 0.15 -2.69 2.98 1.86 0.41 -2.90 -4.98 -4.20 1.85 -1.77 -1.87 -5.65 0.11
Nuclear Periphery Foci 1.39 -8.91 -2.89 -5.96 8.38 3.10 -4.27 2.12 -6.93 10.39 3.00 -8.32 1.69 -6.44 12.10
Nucleolus -0.16 2.82 1.86 3.74 -3.32 1.86 2.24 1.71 -1.19 -4.89 1.61 2.50 1.88 1.67 -5.63
Nucleus -0.75 -3.16 -0.46 0.13 2.93 6.08 4.50 -2.33 -11.62 -9.22 5.07 1.92 -2.43 -9.96 -5.00
Peroxisomes -1.01 6.23 0.03 0.35 -1.68 -6.12 5.61 1.14 5.79 -2.04 -6.05 7.46 0.99 4.85 -2.69
Vacuole 4.18 -1.88 -14.79 -20.44 -11.14 7.01 -14.68 -21.93 -29.24 -0.37 7.07 -14.14 -25.83 -33.99 -4.58
Vacuole Periphery -0.76 2.95 4.84 7.72 2.95 -4.74 9.79 2.12 5.09 -1.96 -5.00 9.14 3.35 6.66 -1.25
Standard name
Human Ortholog
Description Microtubule-associated protein involved in microtubule organization; involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Mhp1

Mhp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mhp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available