Standard name
Human Ortholog
Description Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; role in anaphase progression; required for recovery after DSB repair; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.8 0.89 0.82 0.98 0.94 0.88 0.94 0.8 0.74 0.65 0.72 0.78 0.87 0.91 0.78 0.65 0.69 0.65 0.67 0.68 0.65 0.69 0.66
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.06 0.17 0.07 0.37 0.37 0.54 0.43 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0.18 0.09 0.07 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.33 0.17 0.13 0.13 0.05 0 0 0 0 0 0 0 0.25 0.17 0.12 0.25 0.37 0.35 0.07 0.11 0.14 0.17 0.16 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 3 0 2 4 7
Bud 1 2 0 1 0 2 5 0 2 10 6 4 2 1 2 0 0 0 0 1 0 6 9 21
Bud Neck 0 1 1 0 0 0 0 9 1 0 1 2 0 0 0 0 0 0 1 2 1 2 6 15
Bud Site 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 1 0 1 1 1 1 0 1 0 0 0 0 0 1 0 0 1 0
Cytoplasm 51 326 113 65 103 234 246 289 239 181 147 124 352 419 475 142 132 134 75 343 127 164 364 384
Endoplasmic Reticulum 2 7 1 1 0 0 0 1 0 0 0 0 2 1 1 5 12 14 0 1 0 3 7 6
Endosome 1 5 0 0 1 0 0 0 0 0 0 1 7 14 12 2 10 10 1 7 0 1 3 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 1 0 0 2 0 0 1 1
Mitochondria 1 19 0 2 0 15 47 20 111 92 123 74 6 1 2 4 4 4 1 17 9 10 15 9
Nucleus 7 22 5 3 0 4 3 4 6 7 6 5 4 2 1 12 10 10 20 48 13 8 16 17
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 2
Nucleolus 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 1 1 1 0 1 2 0 0 0 0 0 0 1 12 3 4 5 6
Vac/Vac Membrane 25 68 16 10 5 5 5 8 4 10 7 5 113 83 65 46 75 67 8 58 26 43 83 92
Unique Cell Count 76 410 127 79 105 249 278 306 299 246 227 173 451 479 524 181 204 193 115 516 187 253 529 586
Labelled Cell Count 88 453 136 82 109 261 308 332 367 302 292 218 489 522 560 214 246 239 115 516 187 253 529 586


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.4 5.4 5.0 5.8 4.3 4.4 4.7 3.6 3.6 3.7 4.2 5.4 5.4 5.4 7.1 7.0 6.6 5.0 5.2 5.3
Std Deviation (1e-4) 0.9 0.5 0.9 1.5 2.0 1.5 1.3 1.5 0.8 0.8 1.0 1.2 1.1 1.0 1.1 1.1 1.4 1.4 1.2 2.2 1.5
Intensity Change (Log2) – – – -0.1 0.1 -0.31 -0.28 -0.2 -0.57 -0.58 -0.55 -0.37 -0.01 0.01 0.01 0.4 0.38 0.29 -0.11 -0.06 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.8 4.8 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5245 2.6096 2.54 2.2704 2.6191 2.3215 1.3959 1.2141 1.2637 0.8816 1.6407 1.2932 0.2873 0.6229 0.3925 0.7181 0.1985 0.4603
Actin 0.0347 0.0003 0.0096 0.0025 0.0343 0.0034 0.0068 0.0008 0.0068 0.0302 0.0358 0.0144 0.0136 0.0006 0.0011 0.0002 0.0029 0.0007
Bud 0.0014 0.002 0.0012 0.0004 0.001 0.0013 0.0037 0.0044 0.0022 0.0008 0.0032 0.0024 0.0003 0.0002 0.0006 0.0002 0.0106 0.0002
Bud Neck 0.008 0.0005 0.0034 0.0074 0.003 0.0028 0.0021 0.0008 0.0033 0.0031 0.0021 0.0066 0.002 0.0003 0.0008 0.0009 0.001 0.0027
Bud Periphery 0.0031 0.0015 0.0023 0.0003 0.0021 0.0053 0.0023 0.003 0.0046 0.0007 0.0078 0.0044 0.0003 0.0001 0.0004 0.0001 0.0064 0.0001
Bud Site 0.0059 0.0052 0.017 0.0041 0.0052 0.0007 0.0053 0.0057 0.0025 0.0032 0.0063 0.0022 0.0037 0.0013 0.0011 0.0003 0.0025 0.0002
Cell Periphery 0.001 0.0001 0.0007 0.0001 0.0007 0.0002 0.0004 0.0002 0.0002 0.0001 0.0008 0.0001 0.0002 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.3355 0.5026 0.3578 0.3499 0.3717 0.4522 0.5442 0.6171 0.4967 0.5467 0.5508 0.5695 0.4929 0.6142 0.4896 0.4739 0.5006 0.5178
Cytoplasmic Foci 0.0308 0.0048 0.0186 0.019 0.0433 0.0242 0.0231 0.0121 0.0118 0.043 0.0357 0.0159 0.0172 0.0062 0.0063 0.0047 0.0122 0.0165
Eisosomes 0.0007 0.0001 0.0003 0.0001 0.0004 0.0001 0.0001 0 0.0001 0.0005 0.0003 0.0001 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0088 0.0029 0.0049 0.0038 0.0063 0.0047 0.0173 0.0044 0.0104 0.0041 0.0059 0.0048 0.0071 0.004 0.0046 0.0037 0.0028 0.003
Endosome 0.0254 0.0019 0.0177 0.0079 0.0393 0.0093 0.0371 0.0084 0.0127 0.0181 0.0422 0.0118 0.0122 0.0042 0.0049 0.008 0.0125 0.0066
Golgi 0.0081 0.0001 0.0097 0.0055 0.0161 0.001 0.0043 0.0005 0.004 0.0044 0.0169 0.0032 0.002 0.0002 0.0002 0.0001 0.0021 0.0006
Lipid Particles 0.0161 0.0001 0.0072 0.0163 0.0141 0.001 0.0143 0.0002 0.0081 0.0031 0.0108 0.0031 0.0022 0.0002 0.0002 0.0001 0.0057 0.0012
Mitochondria 0.0139 0.0005 0.0267 0.0039 0.038 0.009 0.0072 0.0009 0.0124 0.0016 0.0472 0.0038 0.0012 0.0006 0.0008 0.001 0.0017 0.0007
None 0.3514 0.3727 0.3659 0.3819 0.2611 0.3568 0.179 0.2303 0.2305 0.142 0.1529 0.1836 0.2937 0.2738 0.3049 0.2742 0.3313 0.2086
Nuclear Periphery 0.0158 0.0033 0.0121 0.0049 0.006 0.0064 0.0374 0.0072 0.0109 0.0084 0.0076 0.0174 0.015 0.0055 0.0094 0.0135 0.0109 0.009
Nucleolus 0.0041 0.0005 0.0026 0.0009 0.0027 0.0011 0.0013 0.0007 0.006 0.0011 0.0008 0.0011 0.0024 0.0006 0.001 0.0014 0.0013 0.001
Nucleus 0.0701 0.094 0.0974 0.1597 0.0875 0.0848 0.0878 0.0898 0.147 0.1338 0.0478 0.1334 0.1018 0.0777 0.1597 0.2028 0.0709 0.2007
Peroxisomes 0.0257 0.0001 0.0125 0.0125 0.043 0.0034 0.0025 0.0003 0.0043 0.0149 0.0064 0.0027 0.006 0.0002 0.0003 0.0001 0.0009 0.0007
Punctate Nuclear 0.0329 0.0056 0.0288 0.0175 0.016 0.0296 0.0126 0.0103 0.021 0.0365 0.0082 0.0158 0.0221 0.0078 0.0118 0.0103 0.0211 0.0278
Vacuole 0.005 0.001 0.0025 0.0011 0.0071 0.002 0.0069 0.0027 0.0038 0.003 0.0062 0.0023 0.0031 0.002 0.002 0.0037 0.0015 0.0014
Vacuole Periphery 0.0016 0.0001 0.0012 0.0003 0.001 0.0005 0.0043 0.0004 0.0008 0.0006 0.0042 0.0013 0.0007 0.0003 0.0004 0.0008 0.0008 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0349 5.0703 5.4989 11.0342 7.071 7.8491 9.3659 9.9811 8.8918 12.6186
Translational Efficiency 0.4883 0.8838 0.9357 0.5055 0.6688 0.6521 0.6292 0.4583 0.7024 0.4962

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1107 644 191 1168 1597 2197 1929 1711 2704 2841 2120 2879

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 652.78 776.79 948.72 895.70 696.46 760.89 868.22 880.17 678.58 764.49 875.47 886.47
Standard Deviation 76.19 122.52 95.40 126.91 83.78 95.51 103.31 137.99 83.56 102.48 105.18 133.82
Intensity Change Log 2 — 0.250928 0.539386 0.456419 — 0.127647 0.318020 0.337742 — 0.188608 0.429364 0.396379

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000083 0.000704 0.001233 0.008262 0.000072 0.000247 0.000593 0.001514 0.000077 0.000350 0.000650 0.004252
Bud Neck 0.004105 0.006455 0.004164 0.020944 0.004171 0.017568 0.007939 0.029233 0.004144 0.015049 0.007599 0.025870
Bud Site 0.002393 0.006495 0.002294 0.053089 0.001966 0.009916 0.005530 0.045876 0.002141 0.009141 0.005238 0.048802
Cell Periphery 0.000109 0.000261 0.000155 0.000310 0.000087 0.000106 0.000136 0.000199 0.000096 0.000141 0.000138 0.000244
Cytoplasm 0.393942* 0.412035* 0.050177 0.193899* 0.457819* 0.436318* 0.164158* 0.282815* 0.431668* 0.430813* 0.153889* 0.246742*
Cytoplasmic Foci 0.073506 0.106594 0.001329 0.005199 0.080276 0.078741 0.001956 0.013785 0.077504 0.085055 0.001899 0.010302
Eisosomes 0.000061 0.000068 0.000080 0.000042 0.000044 0.000049 0.000022 0.000033 0.000051 0.000053 0.000028 0.000037
Endoplasmic Reticulum 0.000935 0.003178 0.016991 0.006036 0.000955 0.000917 0.002589 0.002984 0.000947 0.001430 0.003887 0.004222
Endosome 0.002327 0.007542 0.001994 0.007464 0.002183 0.003810 0.000963 0.015889 0.002242 0.004656 0.001056 0.012471
Golgi 0.000846 0.001966 0.000105 0.005642 0.000488 0.001271 0.000298 0.010990 0.000634 0.001428 0.000281 0.008820
Lipid Particles 0.004419 0.005974 0.001974 0.001040 0.003449 0.002968 0.000462 0.002198 0.003846 0.003649 0.000599 0.001728
Mitochondria 0.003058 0.002307 0.001021 0.010514 0.001996 0.007353 0.001234 0.008038 0.002431 0.006209 0.001215 0.009043
Mitotic Spindle 0.002646 0.005781 0.002840 0.061943 0.003630 0.006392 0.007279 0.100117* 0.003227 0.006254 0.006879 0.084630
None 0.006900 0.005852 0.002399 0.012870 0.004890 0.005512 0.008587 0.012957 0.005713 0.005589 0.008030 0.012922
Nuclear Periphery 0.001037 0.001230 0.002793 0.002861 0.000948 0.000934 0.001868 0.003978 0.000984 0.001001 0.001952 0.003525
Nuclear Periphery Foci 0.000629 0.002184 0.001359 0.001683 0.000484 0.000344 0.000241 0.002380 0.000544 0.000761 0.000342 0.002097
Nucleolus 0.001568 0.003336 0.001508 0.000549 0.001088 0.002160 0.000803 0.001487 0.001285 0.002426 0.000866 0.001106
Nucleus 0.484415* 0.372304* 0.864310* 0.562351* 0.415958* 0.392097* 0.779937* 0.374959* 0.443984* 0.387610* 0.787538* 0.450983*
Peroxisomes 0.001738 0.003219 0.000319 0.001110 0.001832 0.005582 0.001202 0.002696 0.001794 0.005046 0.001122 0.002053
Vacuole 0.014864 0.051391 0.042740 0.041983 0.017367 0.027078 0.014028 0.076004 0.016342 0.032589 0.016615 0.062202
Vacuole Periphery 0.000418 0.001124 0.000218 0.002207 0.000297 0.000640 0.000174 0.011869 0.000347 0.000749 0.000178 0.007949

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.84 -11.68 -3.07 -2.08 -0.88 -4.31 -4.32 -4.07 -2.96 -1.36 -3.33 -5.89 -4.17 -3.24 -2.45
Bud Neck -2.26 -2.62 -8.60 -7.31 -6.95 -9.70 -4.48 -9.43 0.25 -5.41 -9.84 -4.74 -12.75 -1.84 -7.28
Bud Site -2.42 -2.07 -6.83 -4.75 -6.16 -6.40 -3.00 -8.55 -3.94 -7.02 -6.70 -3.05 -10.90 -5.72 -9.04
Cell Periphery -1.49 -5.03 -4.83 0.66 0.41 -2.17 -2.59 -4.68 -2.20 -1.43 -1.83 -2.73 -6.62 -0.53 -3.18
Cytoplasm -0.53 31.23 27.23 22.13 -9.92 2.56 42.44 27.06 26.36 -13.78 0.54 48.45 37.47 37.06 -12.38
Cytoplasmic Foci -4.40 19.27 19.12 17.55 -1.38 0.40 25.03 23.52 25.77 -5.87 -1.91 31.55 30.30 31.42 -5.85
Eisosomes -1.37 -4.37 0.90 3.75 6.16 -1.30 4.94 0.12 1.60 -6.24 -0.74 3.39 0.87 2.37 -4.63
Endoplasmic Reticulum -5.30 -14.60 -19.35 -9.48 8.42 0.22 -17.39 -17.16 -17.20 0.77 -4.13 -20.56 -25.25 -20.79 -0.43
Endosome -6.11 -0.78 -0.07 5.28 0.52 -4.22 5.06 -3.19 -0.81 -5.27 -6.75 5.19 -2.66 2.01 -5.30
Golgi -1.09 1.76 -0.55 0.69 -2.13 -2.36 1.53 -2.92 -1.75 -3.44 -2.23 2.14 -2.79 -1.12 -3.94
Lipid Particles -1.72 3.12 5.45 6.71 3.31 1.12 8.54 5.62 5.79 -3.44 0.47 10.03 7.81 9.05 -4.22
Mitochondria 0.70 1.65 -0.79 -1.85 -3.15 -5.54 1.31 -3.12 3.58 -5.95 -4.49 2.05 -2.40 2.70 -5.93
Mitotic Spindle -1.44 -0.85 -8.41 -7.33 -7.78 -1.71 -3.91 -12.41 -11.78 -10.14 -2.36 -4.44 -14.97 -13.82 -11.54
None 1.01 6.65 -2.42 -3.32 -8.64 -0.94 -2.11 -5.90 -5.78 -3.60 0.22 -0.75 -6.10 -6.81 -4.49
Nuclear Periphery -1.70 -9.73 -17.04 -16.59 -8.82 -0.10 -16.61 -15.95 -15.97 -6.75 -0.64 -17.32 -23.12 -23.27 -10.96
Nuclear Periphery Foci -4.00 -3.93 -4.54 2.08 0.79 2.41 0.42 -4.11 -5.35 -4.34 -2.22 -0.36 -6.07 -2.95 -5.90
Nucleolus -2.14 1.12 2.00 3.12 1.28 -3.52 1.07 -1.29 2.82 -3.20 -3.83 2.22 0.26 4.48 -2.76
Nucleus 7.76 -18.44 -12.29 -18.40 10.09 2.54 -43.34 -9.33 -12.20 29.83 7.35 -46.94 -14.74 -22.00 30.11
Peroxisomes -2.79 4.45 4.44 6.68 0.32 -4.64 2.07 2.02 6.76 -0.23 -5.40 2.73 3.53 8.21 0.09
Vacuole -9.87 -7.17 -18.48 -6.78 -1.86 -5.16 -6.53 -22.76 -20.57 -18.19 -10.71 -9.53 -29.23 -22.50 -19.87
Vacuole Periphery -2.91 1.48 -0.46 1.64 -1.13 -3.86 2.06 -4.29 -3.73 -4.59 -4.55 3.01 -4.23 -3.17 -4.81
Standard name
Human Ortholog
Description Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; role in anaphase progression; required for recovery after DSB repair; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
Localization
Cell Percentages cytoplasm (28%), nucleus (12%), mixed (47%)
Cell Cycle Regulation Yes
nucleus - S/G2 (1.4e-06)
Subcompartmental Group N/A

Rtt101

Rtt101


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtt101-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available