Standard name | |
---|---|
Human Ortholog | |
Description | ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 3 | 3 | 1 | 1 | 8 | 18 | 39 |
Bud | 0 | 5 | 3 | 3 | 1 | 1 | 1 | 9 | 2 | – | 11 | 8 | 0 | 3 | 15 | 1 | 1 | 1 | 0 | 1 | 0 | 2 | 8 | 13 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 1 | 9 | 18 | 17 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 3 | 2 | 1 | 2 | 6 | 4 | 8 | 9 | – | 2 | 5 | 7 | 3 | 5 | 23 | 18 | 18 | 0 | 0 | 0 | 0 | 1 | 3 |
Cytoplasm | 189 | 251 | 108 | 149 | 213 | 214 | 284 | 377 | 215 | – | 204 | 249 | 269 | 399 | 468 | 451 | 261 | 392 | 182 | 240 | 101 | 136 | 234 | 249 |
Endoplasmic Reticulum | 4 | 6 | 2 | 0 | 3 | 0 | 3 | 3 | 3 | – | 1 | 3 | 2 | 3 | 4 | 18 | 11 | 22 | 6 | 1 | 1 | 6 | 6 | 20 |
Endosome | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 0 | 3 | 4 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 |
Mitochondria | 2 | 3 | 1 | 11 | 3 | 25 | 54 | 82 | 70 | – | 118 | 142 | 1 | 0 | 6 | 2 | 4 | 5 | 2 | 3 | 7 | 2 | 9 | 14 |
Nucleus | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 1 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 1 | 5 | 0 | 0 | 0 | 10 | 4 | 9 | 1 | 5 | 14 | 10 | 9 | 9 |
Unique Cell Count | 197 | 262 | 111 | 149 | 218 | 219 | 311 | 428 | 251 | 263 | 314 | 272 | 402 | 477 | 470 | 270 | 410 | 206 | 266 | 132 | 182 | 318 | 384 | |
Labelled Cell Count | 209 | 269 | 116 | 164 | 226 | 247 | 347 | 483 | 301 | 339 | 416 | 280 | 409 | 500 | 513 | 303 | 455 | 206 | 266 | 132 | 182 | 318 | 384 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 6.0 | 4.5 | 4.5 | 4.9 | 4.6 | 4.4 | 4.4 | 4.0 | – | 4.0 | 3.7 | 6.4 | 6.3 | 5.5 | 7.3 | 8.2 | 8.2 | 6.6 | 6.0 | 6.8 |
Std Deviation (1e-4) | 0.8 | 0.8 | 0.6 | 0.8 | 0.9 | 1.0 | 0.8 | 0.8 | 0.7 | – | 1.0 | 0.8 | 0.8 | 1.0 | 1.1 | 1.1 | 1.7 | 1.4 | 1.6 | 1.2 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.13 | 0.03 | -0.05 | -0.04 | -0.16 | – | -0.16 | -0.3 | 0.5 | 0.49 | 0.28 | 0.7 | 0.85 | 0.85 | 0.54 | 0.42 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 |
Cytoplasm | 2.0 | 0.2 | 0.2 | -2.1 | -2.9 | -3.3 | – | -4.7 | -4.4 | 1.1 | 1.7 | 0.5 | -0.7 | -0.3 | -0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1632 | 3.4892 | 3.1853 | 3.1195 | 3.1037 | 3.2806 | 3.4695 | 3.903 | 3.9066 | 3.6934 | 3.1984 | 3.714 | 3.3969 | 3.5578 | 3.3825 | 3.3434 | 3.0576 | 3.4658 |
Actin | 0.0423 | 0.0019 | 0.0071 | 0.0423 | 0.0032 | 0.0038 | 0.03 | 0.0029 | 0.0168 | 0.0024 | 0.0051 | 0.0125 | 0.0509 | 0.0049 | 0.0097 | 0.0634 | 0.0018 | 0.0168 |
Bud | 0.0008 | 0.0022 | 0.0019 | 0.0012 | 0.0002 | 0.0009 | 0.0013 | 0.0063 | 0.0026 | 0.0061 | 0.0107 | 0.0014 | 0.001 | 0.0045 | 0.0052 | 0.0009 | 0.0003 | 0.0013 |
Bud Neck | 0.0036 | 0.0006 | 0.0013 | 0.0015 | 0.0008 | 0.0139 | 0.0019 | 0.0005 | 0.0047 | 0.0005 | 0.0029 | 0.0031 | 0.0021 | 0.0006 | 0.0045 | 0.009 | 0.0008 | 0.0079 |
Bud Periphery | 0.0015 | 0.0014 | 0.004 | 0.0014 | 0.0007 | 0.0007 | 0.001 | 0.0041 | 0.0032 | 0.0137 | 0.0076 | 0.0024 | 0.001 | 0.0061 | 0.005 | 0.0013 | 0.0001 | 0.0033 |
Bud Site | 0.0064 | 0.0257 | 0.0026 | 0.0037 | 0.0002 | 0.0003 | 0.019 | 0.0144 | 0.0178 | 0.0042 | 0.0181 | 0.0006 | 0.0115 | 0.0206 | 0.0128 | 0.0049 | 0.0038 | 0.0005 |
Cell Periphery | 0.0038 | 0.0013 | 0.0009 | 0.0022 | 0.0021 | 0.0005 | 0.0014 | 0.001 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0008 | 0.001 | 0.0012 | 0.0023 | 0.0001 | 0.0006 |
Cytoplasm | 0.621 | 0.8194 | 0.7807 | 0.7441 | 0.8018 | 0.865 | 0.6162 | 0.8402 | 0.784 | 0.8035 | 0.8361 | 0.8431 | 0.6169 | 0.8154 | 0.7626 | 0.717 | 0.9074 | 0.8184 |
Cytoplasmic Foci | 0.0279 | 0.0086 | 0.0261 | 0.0196 | 0.0179 | 0.0206 | 0.0565 | 0.0068 | 0.0119 | 0.0187 | 0.0109 | 0.013 | 0.0484 | 0.0184 | 0.0225 | 0.0307 | 0.0315 | 0.0114 |
Eisosomes | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0168 | 0.0055 | 0.0055 | 0.0062 | 0.0056 | 0.0091 | 0.026 | 0.0035 | 0.004 | 0.0031 | 0.0026 | 0.0078 | 0.0082 | 0.0054 | 0.0041 | 0.0104 | 0.002 | 0.0028 |
Endosome | 0.0374 | 0.0056 | 0.0126 | 0.0199 | 0.0101 | 0.0069 | 0.0568 | 0.0042 | 0.0163 | 0.0422 | 0.014 | 0.0277 | 0.0325 | 0.0052 | 0.0079 | 0.0196 | 0.0089 | 0.0102 |
Golgi | 0.0068 | 0.0008 | 0.0032 | 0.0041 | 0.0009 | 0.0013 | 0.0133 | 0.0008 | 0.0074 | 0.0073 | 0.0042 | 0.0077 | 0.0215 | 0.0016 | 0.0027 | 0.009 | 0.0019 | 0.008 |
Lipid Particles | 0.011 | 0.0004 | 0.0084 | 0.0062 | 0.0013 | 0.0004 | 0.0145 | 0.0005 | 0.0011 | 0.0121 | 0.0044 | 0.003 | 0.0121 | 0.0009 | 0.0014 | 0.0196 | 0.0022 | 0.0006 |
Mitochondria | 0.0045 | 0.0003 | 0.001 | 0.0232 | 0.0008 | 0.0071 | 0.0082 | 0.0046 | 0.0029 | 0.0051 | 0.0055 | 0.0094 | 0.0045 | 0.0003 | 0.0013 | 0.0029 | 0.0004 | 0.0057 |
None | 0.1523 | 0.1163 | 0.1168 | 0.1114 | 0.1446 | 0.0646 | 0.0909 | 0.1034 | 0.1114 | 0.0631 | 0.0701 | 0.0488 | 0.1676 | 0.1099 | 0.1383 | 0.0871 | 0.0249 | 0.1082 |
Nuclear Periphery | 0.0111 | 0.0008 | 0.0021 | 0.0013 | 0.0012 | 0.0004 | 0.0157 | 0.0005 | 0.0029 | 0.0013 | 0.0015 | 0.0044 | 0.0032 | 0.0006 | 0.0012 | 0.0021 | 0.0005 | 0.0004 |
Nucleolus | 0.0028 | 0.0004 | 0.0007 | 0.0004 | 0.0001 | 0.0001 | 0.0024 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.001 | 0.0003 | 0.0003 | 0 |
Nucleus | 0.015 | 0.0019 | 0.0028 | 0.0015 | 0.0012 | 0.0009 | 0.0124 | 0.0011 | 0.0041 | 0.0019 | 0.0012 | 0.001 | 0.001 | 0.0008 | 0.0016 | 0.0011 | 0.0008 | 0.0006 |
Peroxisomes | 0.0169 | 0.0001 | 0.008 | 0.0036 | 0.0034 | 0.0006 | 0.0142 | 0.0003 | 0.0015 | 0.0032 | 0.0012 | 0.0094 | 0.0098 | 0.0007 | 0.0088 | 0.0102 | 0.0028 | 0.0018 |
Punctate Nuclear | 0.0082 | 0.0011 | 0.0094 | 0.0014 | 0.0003 | 0.0006 | 0.0096 | 0.0004 | 0.0009 | 0.0008 | 0.0007 | 0.0008 | 0.0034 | 0.0015 | 0.0006 | 0.0035 | 0.0087 | 0.0002 |
Vacuole | 0.0076 | 0.0053 | 0.0044 | 0.0035 | 0.0034 | 0.002 | 0.007 | 0.0039 | 0.0046 | 0.0085 | 0.002 | 0.0024 | 0.0019 | 0.0012 | 0.007 | 0.0031 | 0.0006 | 0.0011 |
Vacuole Periphery | 0.0015 | 0.0003 | 0.0005 | 0.0009 | 0.0004 | 0.0003 | 0.0014 | 0.0003 | 0.0009 | 0.0014 | 0.0007 | 0.0012 | 0.0007 | 0.0001 | 0.0005 | 0.0007 | 0.0001 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 85.1012 | 92.1475 | 71.7849 | 58.3495 | 77.2584 | 59.2127 | 57.8058 | 50.871 | 45.911 | 66.5278 |
Translational Efficiency | 1.5773 | 1.5741 | 1.4438 | 1.4676 | 1.3612 | 1.3909 | 1.5466 | 1.1091 | 1.0499 | 1.1606 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
517 | 782 | 435 | 905 | 99 | 946 | 169 | 88 | 616 | 1728 | 604 | 993 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 736.61 | 859.96 | 1111.65 | 974.25 | 826.05 | 839.38 | 1080.13 | 1049.08 | 750.98 | 848.69 | 1102.83 | 980.88 |
Standard Deviation | 81.46 | 122.02 | 129.09 | 136.25 | 118.49 | 110.38 | 118.09 | 168.39 | 94.36 | 116.24 | 126.90 | 141.01 |
Intensity Change Log 2 | — | 0.223369 | 0.593730 | 0.403391 | — | 0.023095 | 0.386904 | 0.344824 | — | 0.120971 | 0.488099 | 0.372728 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000393 | 0.001279 | 0.001111 | 0.001814 | 0.002412 | 0.001761 | 0.002469 | 0.001525 | 0.000718 | 0.001543 | 0.001491 | 0.001789 |
Bud Neck | 0.017375 | 0.017987 | 0.002059 | 0.012029 | 0.005272 | 0.023040 | 0.004168 | 0.005333 | 0.015430 | 0.020753 | 0.002649 | 0.011436 |
Bud Site | 0.014795 | 0.044104 | 0.033637 | 0.051286 | 0.020942 | 0.036627 | 0.039853 | 0.074340 | 0.015783 | 0.040011 | 0.035376 | 0.053329 |
Cell Periphery | 0.000260 | 0.000190 | 0.000207 | 0.000177 | 0.000403 | 0.000247 | 0.000484 | 0.000214 | 0.000283 | 0.000221 | 0.000284 | 0.000180 |
Cytoplasm | 0.530619* | 0.487724* | 0.742137* | 0.374453* | 0.488264* | 0.453791* | 0.410525* | 0.393817* | 0.523812* | 0.469147* | 0.649352* | 0.376169* |
Cytoplasmic Foci | 0.209524* | 0.247103* | 0.072660 | 0.054432 | 0.276846* | 0.199159* | 0.068260 | 0.086441 | 0.220344* | 0.220856* | 0.071429 | 0.057269 |
Eisosomes | 0.000304 | 0.000181 | 0.000028 | 0.000079 | 0.000273 | 0.000233 | 0.000146 | 0.000086 | 0.000299 | 0.000209 | 0.000061 | 0.000079 |
Endoplasmic Reticulum | 0.000747 | 0.001244 | 0.001216 | 0.001894 | 0.004143 | 0.000694 | 0.004758 | 0.002946 | 0.001293 | 0.000943 | 0.002207 | 0.001988 |
Endosome | 0.032046 | 0.037866 | 0.003075 | 0.029811 | 0.043061 | 0.050612 | 0.011878 | 0.012338 | 0.033816 | 0.044844 | 0.005538 | 0.028263 |
Golgi | 0.003984 | 0.009148 | 0.000420 | 0.008617 | 0.002861 | 0.011080 | 0.000317 | 0.000990 | 0.003804 | 0.010206 | 0.000391 | 0.007942 |
Lipid Particles | 0.005633 | 0.003025 | 0.001037 | 0.005442 | 0.010914 | 0.002764 | 0.003970 | 0.018458 | 0.006482 | 0.002882 | 0.001858 | 0.006595 |
Mitochondria | 0.003834 | 0.003728 | 0.000099 | 0.005529 | 0.000552 | 0.007010 | 0.000120 | 0.000875 | 0.003307 | 0.005525 | 0.000105 | 0.005117 |
Mitotic Spindle | 0.000582 | 0.003339 | 0.000748 | 0.052810 | 0.000163 | 0.004068 | 0.002914 | 0.003902 | 0.000515 | 0.003738 | 0.001354 | 0.048475 |
None | 0.000776 | 0.000430 | 0.001347 | 0.000422 | 0.000398 | 0.000340 | 0.000905 | 0.000395 | 0.000715 | 0.000381 | 0.001224 | 0.000419 |
Nuclear Periphery | 0.000164 | 0.000064 | 0.000144 | 0.000835 | 0.000612 | 0.000091 | 0.000723 | 0.000713 | 0.000236 | 0.000078 | 0.000306 | 0.000824 |
Nuclear Periphery Foci | 0.000806 | 0.000615 | 0.009150 | 0.002599 | 0.005486 | 0.000337 | 0.013725 | 0.005894 | 0.001558 | 0.000463 | 0.010430 | 0.002891 |
Nucleolus | 0.000426 | 0.000200 | 0.000074 | 0.000841 | 0.000913 | 0.000893 | 0.000163 | 0.000062 | 0.000504 | 0.000579 | 0.000099 | 0.000772 |
Nucleus | 0.026599 | 0.012754 | 0.048784 | 0.037474 | 0.030644 | 0.013874 | 0.056751 | 0.015795 | 0.027249 | 0.013367 | 0.051013 | 0.035553 |
Peroxisomes | 0.003607 | 0.008729 | 0.000860 | 0.005745 | 0.005870 | 0.008069 | 0.001895 | 0.002638 | 0.003971 | 0.008368 | 0.001150 | 0.005470 |
Vacuole | 0.145901 | 0.117800 | 0.080992 | 0.346255* | 0.097112 | 0.180776 | 0.375719* | 0.372796* | 0.138060 | 0.152277 | 0.163457 | 0.348607* |
Vacuole Periphery | 0.001624 | 0.002490 | 0.000215 | 0.007454 | 0.002859 | 0.004533 | 0.000257 | 0.000441 | 0.001822 | 0.003608 | 0.000227 | 0.006833 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.28 | -2.49 | -2.52 | 0.14 | -0.20 | 0.48 | 0.30 | 0.86 | 0.84 | 1.28 | -1.97 | -1.84 | -1.30 | 0.71 | 0.41 |
Bud Neck | -0.15 | 9.73 | 5.26 | 5.42 | -4.13 | -9.05 | 1.10 | 1.33 | 10.41 | 0.50 | -2.92 | 9.52 | 5.05 | 8.74 | -3.74 |
Bud Site | -8.63 | -3.27 | -4.95 | 2.23 | -0.36 | -2.04 | -0.97 | -1.73 | -0.83 | -1.23 | -9.54 | -3.65 | -4.98 | 1.29 | -0.75 |
Cell Periphery | 3.32 | 2.19 | 6.30 | 3.40 | 3.59 | 1.58 | 0.19 | 2.49 | 2.58 | 4.23 | 2.35 | 0.92 | 6.28 | 4.49 | 5.64 |
Cytoplasm | 3.10 | -12.43 | 11.38 | 8.51 | 24.01 | 1.31 | 3.91 | 4.21 | 4.16 | 1.00 | 4.80 | -6.15 | 11.91 | 9.04 | 17.00 |
Cytoplasmic Foci | -3.66 | 16.58 | 22.44 | 28.50 | 6.46 | 4.27 | 12.19 | 10.49 | 12.05 | -1.24 | 0.10 | 20.52 | 25.02 | 36.42 | 5.75 |
Eisosomes | 3.68 | 9.91 | 8.77 | 5.74 | -5.58 | 0.45 | 1.40 | 2.05 | 6.25 | 2.62 | 2.88 | 8.34 | 8.43 | 8.96 | -0.21 |
Endoplasmic Reticulum | -1.92 | -2.90 | -1.29 | 0.09 | 0.23 | 2.06 | -0.28 | 1.46 | -1.75 | 3.99 | 1.13 | -2.63 | 0.12 | -0.95 | 2.74 |
Endosome | -1.52 | 10.70 | 4.93 | 7.03 | -5.70 | -1.09 | 6.58 | 6.52 | 12.57 | 0.76 | -3.46 | 12.02 | 6.29 | 11.21 | -5.04 |
Golgi | -3.98 | 4.09 | 0.01 | 3.94 | -3.96 | -4.84 | 4.42 | 3.83 | 6.68 | -0.55 | -5.15 | 4.64 | 0.13 | 5.05 | -4.01 |
Lipid Particles | 3.18 | 5.84 | 1.83 | -0.28 | -2.04 | 2.54 | 2.26 | 0.63 | -0.83 | -0.66 | 4.31 | 5.56 | 2.15 | -0.72 | -1.68 |
Mitochondria | 0.08 | 1.90 | 0.58 | 0.72 | -4.07 | -4.23 | 2.38 | 0.81 | 4.36 | -0.65 | -1.09 | 1.93 | 0.51 | 2.62 | -4.08 |
Mitotic Spindle | -2.38 | -0.46 | -6.59 | -5.83 | -6.55 | -3.65 | -1.52 | -2.63 | -0.22 | -0.61 | -3.85 | -1.40 | -6.68 | -5.82 | -6.42 |
None | 1.69 | -1.81 | 1.75 | -0.38 | 3.78 | 0.40 | -2.48 | -0.20 | -0.48 | 2.10 | 2.28 | -2.35 | 1.62 | -1.08 | 4.31 |
Nuclear Periphery | 2.76 | -0.45 | -4.11 | -5.08 | -4.12 | 1.70 | -0.86 | 0.17 | -2.63 | 1.76 | 2.72 | -2.24 | -3.49 | -5.29 | -2.61 |
Nuclear Periphery Foci | 0.70 | -10.06 | -2.76 | -3.59 | 8.14 | 2.79 | -2.45 | 0.55 | -3.19 | 3.91 | 2.99 | -10.82 | -1.32 | -4.76 | 9.89 |
Nucleolus | 4.68 | 8.15 | -0.27 | -1.52 | -2.22 | -0.06 | 2.34 | 2.65 | 1.48 | 2.53 | -0.32 | 6.35 | 0.37 | 0.46 | -2.08 |
Nucleus | 5.07 | -6.26 | -2.14 | -7.63 | 4.18 | 1.90 | -3.70 | 1.69 | -0.53 | 7.33 | 5.52 | -7.52 | -1.57 | -8.07 | 6.32 |
Peroxisomes | -5.53 | 6.76 | 0.74 | 4.87 | -2.58 | -0.76 | 1.50 | 1.46 | 6.33 | -0.15 | -5.42 | 5.11 | 1.23 | 5.77 | -2.36 |
Vacuole | 2.92 | 3.78 | -25.08 | -27.61 | -26.36 | -7.71 | -15.28 | -12.60 | -10.18 | -1.86 | -2.91 | -5.91 | -27.99 | -27.27 | -17.79 |
Vacuole Periphery | -2.03 | 7.01 | -2.06 | -1.11 | -3.67 | -1.49 | 3.96 | 3.90 | 4.99 | -0.19 | -3.28 | 8.06 | -1.66 | 0.34 | -3.67 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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