Standard name
Human Ortholog
Description ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.1
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.97 1.0 0.98 0.98 0.91 0.88 0.86 – 0.78 0.79 0.99 0.99 0.98 0.96 0.97 0.96 0.88 0.9 0.77 0.75 0.74 0.65
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0 0.11 0.17 0.19 0.28 – 0.45 0.45 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.11 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 – 0 1 1 0 0 2 0 3 3 1 1 8 18 39
Bud 0 5 3 3 1 1 1 9 2 – 11 8 0 3 15 1 1 1 0 1 0 2 8 13
Bud Neck 0 0 0 0 1 0 0 1 0 – 0 0 0 0 0 0 0 0 2 5 1 9 18 17
Bud Site 0 0 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 3 2 1 2 6 4 8 9 – 2 5 7 3 5 23 18 18 0 0 0 0 1 3
Cytoplasm 189 251 108 149 213 214 284 377 215 – 204 249 269 399 468 451 261 392 182 240 101 136 234 249
Endoplasmic Reticulum 4 6 2 0 3 0 3 3 3 – 1 3 2 3 4 18 11 22 6 1 1 6 6 20
Endosome 3 0 0 0 1 0 0 0 0 – 0 0 0 1 1 1 1 2 2 1 0 3 4 6
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 1 1 1 0 0 1 1
Mitochondria 2 3 1 11 3 25 54 82 70 – 118 142 1 0 6 2 4 5 2 3 7 2 9 14
Nucleus 1 1 0 0 1 1 0 1 0 – 0 1 0 0 1 4 2 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 1 0 1 0 0 – 2 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Vac/Vac Membrane 2 0 0 0 0 0 0 2 1 – 1 5 0 0 0 10 4 9 1 5 14 10 9 9
Unique Cell Count 197 262 111 149 218 219 311 428 251 263 314 272 402 477 470 270 410 206 266 132 182 318 384
Labelled Cell Count 209 269 116 164 226 247 347 483 301 339 416 280 409 500 513 303 455 206 266 132 182 318 384


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 6.0 4.5 4.5 4.9 4.6 4.4 4.4 4.0 – 4.0 3.7 6.4 6.3 5.5 7.3 8.2 8.2 6.6 6.0 6.8
Std Deviation (1e-4) 0.8 0.8 0.6 0.8 0.9 1.0 0.8 0.8 0.7 – 1.0 0.8 0.8 1.0 1.1 1.1 1.7 1.4 1.6 1.2 1.2
Intensity Change (Log2) – – – -0.0 0.13 0.03 -0.05 -0.04 -0.16 – -0.16 -0.3 0.5 0.49 0.28 0.7 0.85 0.85 0.54 0.42 0.59

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 1.9 0
Cytoplasm 2.0 0.2 0.2 -2.1 -2.9 -3.3 – -4.7 -4.4 1.1 1.7 0.5 -0.7 -0.3 -0.8
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1.6
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1632 3.4892 3.1853 3.1195 3.1037 3.2806 3.4695 3.903 3.9066 3.6934 3.1984 3.714 3.3969 3.5578 3.3825 3.3434 3.0576 3.4658
Actin 0.0423 0.0019 0.0071 0.0423 0.0032 0.0038 0.03 0.0029 0.0168 0.0024 0.0051 0.0125 0.0509 0.0049 0.0097 0.0634 0.0018 0.0168
Bud 0.0008 0.0022 0.0019 0.0012 0.0002 0.0009 0.0013 0.0063 0.0026 0.0061 0.0107 0.0014 0.001 0.0045 0.0052 0.0009 0.0003 0.0013
Bud Neck 0.0036 0.0006 0.0013 0.0015 0.0008 0.0139 0.0019 0.0005 0.0047 0.0005 0.0029 0.0031 0.0021 0.0006 0.0045 0.009 0.0008 0.0079
Bud Periphery 0.0015 0.0014 0.004 0.0014 0.0007 0.0007 0.001 0.0041 0.0032 0.0137 0.0076 0.0024 0.001 0.0061 0.005 0.0013 0.0001 0.0033
Bud Site 0.0064 0.0257 0.0026 0.0037 0.0002 0.0003 0.019 0.0144 0.0178 0.0042 0.0181 0.0006 0.0115 0.0206 0.0128 0.0049 0.0038 0.0005
Cell Periphery 0.0038 0.0013 0.0009 0.0022 0.0021 0.0005 0.0014 0.001 0.0005 0.0005 0.0002 0.0002 0.0008 0.001 0.0012 0.0023 0.0001 0.0006
Cytoplasm 0.621 0.8194 0.7807 0.7441 0.8018 0.865 0.6162 0.8402 0.784 0.8035 0.8361 0.8431 0.6169 0.8154 0.7626 0.717 0.9074 0.8184
Cytoplasmic Foci 0.0279 0.0086 0.0261 0.0196 0.0179 0.0206 0.0565 0.0068 0.0119 0.0187 0.0109 0.013 0.0484 0.0184 0.0225 0.0307 0.0315 0.0114
Eisosomes 0.0006 0.0001 0.0001 0.0003 0.0002 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0009 0.0001 0.0001 0.0008 0 0.0001
Endoplasmic Reticulum 0.0168 0.0055 0.0055 0.0062 0.0056 0.0091 0.026 0.0035 0.004 0.0031 0.0026 0.0078 0.0082 0.0054 0.0041 0.0104 0.002 0.0028
Endosome 0.0374 0.0056 0.0126 0.0199 0.0101 0.0069 0.0568 0.0042 0.0163 0.0422 0.014 0.0277 0.0325 0.0052 0.0079 0.0196 0.0089 0.0102
Golgi 0.0068 0.0008 0.0032 0.0041 0.0009 0.0013 0.0133 0.0008 0.0074 0.0073 0.0042 0.0077 0.0215 0.0016 0.0027 0.009 0.0019 0.008
Lipid Particles 0.011 0.0004 0.0084 0.0062 0.0013 0.0004 0.0145 0.0005 0.0011 0.0121 0.0044 0.003 0.0121 0.0009 0.0014 0.0196 0.0022 0.0006
Mitochondria 0.0045 0.0003 0.001 0.0232 0.0008 0.0071 0.0082 0.0046 0.0029 0.0051 0.0055 0.0094 0.0045 0.0003 0.0013 0.0029 0.0004 0.0057
None 0.1523 0.1163 0.1168 0.1114 0.1446 0.0646 0.0909 0.1034 0.1114 0.0631 0.0701 0.0488 0.1676 0.1099 0.1383 0.0871 0.0249 0.1082
Nuclear Periphery 0.0111 0.0008 0.0021 0.0013 0.0012 0.0004 0.0157 0.0005 0.0029 0.0013 0.0015 0.0044 0.0032 0.0006 0.0012 0.0021 0.0005 0.0004
Nucleolus 0.0028 0.0004 0.0007 0.0004 0.0001 0.0001 0.0024 0.0003 0.0004 0.0003 0.0003 0.0001 0.0003 0.0001 0.001 0.0003 0.0003 0
Nucleus 0.015 0.0019 0.0028 0.0015 0.0012 0.0009 0.0124 0.0011 0.0041 0.0019 0.0012 0.001 0.001 0.0008 0.0016 0.0011 0.0008 0.0006
Peroxisomes 0.0169 0.0001 0.008 0.0036 0.0034 0.0006 0.0142 0.0003 0.0015 0.0032 0.0012 0.0094 0.0098 0.0007 0.0088 0.0102 0.0028 0.0018
Punctate Nuclear 0.0082 0.0011 0.0094 0.0014 0.0003 0.0006 0.0096 0.0004 0.0009 0.0008 0.0007 0.0008 0.0034 0.0015 0.0006 0.0035 0.0087 0.0002
Vacuole 0.0076 0.0053 0.0044 0.0035 0.0034 0.002 0.007 0.0039 0.0046 0.0085 0.002 0.0024 0.0019 0.0012 0.007 0.0031 0.0006 0.0011
Vacuole Periphery 0.0015 0.0003 0.0005 0.0009 0.0004 0.0003 0.0014 0.0003 0.0009 0.0014 0.0007 0.0012 0.0007 0.0001 0.0005 0.0007 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 85.1012 92.1475 71.7849 58.3495 77.2584 59.2127 57.8058 50.871 45.911 66.5278
Translational Efficiency 1.5773 1.5741 1.4438 1.4676 1.3612 1.3909 1.5466 1.1091 1.0499 1.1606

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
517 782 435 905 99 946 169 88 616 1728 604 993

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 736.61 859.96 1111.65 974.25 826.05 839.38 1080.13 1049.08 750.98 848.69 1102.83 980.88
Standard Deviation 81.46 122.02 129.09 136.25 118.49 110.38 118.09 168.39 94.36 116.24 126.90 141.01
Intensity Change Log 2 — 0.223369 0.593730 0.403391 — 0.023095 0.386904 0.344824 — 0.120971 0.488099 0.372728

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000393 0.001279 0.001111 0.001814 0.002412 0.001761 0.002469 0.001525 0.000718 0.001543 0.001491 0.001789
Bud Neck 0.017375 0.017987 0.002059 0.012029 0.005272 0.023040 0.004168 0.005333 0.015430 0.020753 0.002649 0.011436
Bud Site 0.014795 0.044104 0.033637 0.051286 0.020942 0.036627 0.039853 0.074340 0.015783 0.040011 0.035376 0.053329
Cell Periphery 0.000260 0.000190 0.000207 0.000177 0.000403 0.000247 0.000484 0.000214 0.000283 0.000221 0.000284 0.000180
Cytoplasm 0.530619 0.487724 0.742137 0.374453 0.488264 0.453791 0.410525 0.393817 0.523812 0.469147 0.649352 0.376169
Cytoplasmic Foci 0.209524 0.247103 0.072660 0.054432 0.276846 0.199159 0.068260 0.086441 0.220344 0.220856 0.071429 0.057269
Eisosomes 0.000304 0.000181 0.000028 0.000079 0.000273 0.000233 0.000146 0.000086 0.000299 0.000209 0.000061 0.000079
Endoplasmic Reticulum 0.000747 0.001244 0.001216 0.001894 0.004143 0.000694 0.004758 0.002946 0.001293 0.000943 0.002207 0.001988
Endosome 0.032046 0.037866 0.003075 0.029811 0.043061 0.050612 0.011878 0.012338 0.033816 0.044844 0.005538 0.028263
Golgi 0.003984 0.009148 0.000420 0.008617 0.002861 0.011080 0.000317 0.000990 0.003804 0.010206 0.000391 0.007942
Lipid Particles 0.005633 0.003025 0.001037 0.005442 0.010914 0.002764 0.003970 0.018458 0.006482 0.002882 0.001858 0.006595
Mitochondria 0.003834 0.003728 0.000099 0.005529 0.000552 0.007010 0.000120 0.000875 0.003307 0.005525 0.000105 0.005117
Mitotic Spindle 0.000582 0.003339 0.000748 0.052810 0.000163 0.004068 0.002914 0.003902 0.000515 0.003738 0.001354 0.048475
None 0.000776 0.000430 0.001347 0.000422 0.000398 0.000340 0.000905 0.000395 0.000715 0.000381 0.001224 0.000419
Nuclear Periphery 0.000164 0.000064 0.000144 0.000835 0.000612 0.000091 0.000723 0.000713 0.000236 0.000078 0.000306 0.000824
Nuclear Periphery Foci 0.000806 0.000615 0.009150 0.002599 0.005486 0.000337 0.013725 0.005894 0.001558 0.000463 0.010430 0.002891
Nucleolus 0.000426 0.000200 0.000074 0.000841 0.000913 0.000893 0.000163 0.000062 0.000504 0.000579 0.000099 0.000772
Nucleus 0.026599 0.012754 0.048784 0.037474 0.030644 0.013874 0.056751 0.015795 0.027249 0.013367 0.051013 0.035553
Peroxisomes 0.003607 0.008729 0.000860 0.005745 0.005870 0.008069 0.001895 0.002638 0.003971 0.008368 0.001150 0.005470
Vacuole 0.145901 0.117800 0.080992 0.346255 0.097112 0.180776 0.375719 0.372796 0.138060 0.152277 0.163457 0.348607
Vacuole Periphery 0.001624 0.002490 0.000215 0.007454 0.002859 0.004533 0.000257 0.000441 0.001822 0.003608 0.000227 0.006833

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.28 -2.49 -2.52 0.14 -0.20 0.48 0.30 0.86 0.84 1.28 -1.97 -1.84 -1.30 0.71 0.41
Bud Neck -0.15 9.73 5.26 5.42 -4.13 -9.05 1.10 1.33 10.41 0.50 -2.92 9.52 5.05 8.74 -3.74
Bud Site -8.63 -3.27 -4.95 2.23 -0.36 -2.04 -0.97 -1.73 -0.83 -1.23 -9.54 -3.65 -4.98 1.29 -0.75
Cell Periphery 3.32 2.19 6.30 3.40 3.59 1.58 0.19 2.49 2.58 4.23 2.35 0.92 6.28 4.49 5.64
Cytoplasm 3.10 -12.43 11.38 8.51 24.01 1.31 3.91 4.21 4.16 1.00 4.80 -6.15 11.91 9.04 17.00
Cytoplasmic Foci -3.66 16.58 22.44 28.50 6.46 4.27 12.19 10.49 12.05 -1.24 0.10 20.52 25.02 36.42 5.75
Eisosomes 3.68 9.91 8.77 5.74 -5.58 0.45 1.40 2.05 6.25 2.62 2.88 8.34 8.43 8.96 -0.21
Endoplasmic Reticulum -1.92 -2.90 -1.29 0.09 0.23 2.06 -0.28 1.46 -1.75 3.99 1.13 -2.63 0.12 -0.95 2.74
Endosome -1.52 10.70 4.93 7.03 -5.70 -1.09 6.58 6.52 12.57 0.76 -3.46 12.02 6.29 11.21 -5.04
Golgi -3.98 4.09 0.01 3.94 -3.96 -4.84 4.42 3.83 6.68 -0.55 -5.15 4.64 0.13 5.05 -4.01
Lipid Particles 3.18 5.84 1.83 -0.28 -2.04 2.54 2.26 0.63 -0.83 -0.66 4.31 5.56 2.15 -0.72 -1.68
Mitochondria 0.08 1.90 0.58 0.72 -4.07 -4.23 2.38 0.81 4.36 -0.65 -1.09 1.93 0.51 2.62 -4.08
Mitotic Spindle -2.38 -0.46 -6.59 -5.83 -6.55 -3.65 -1.52 -2.63 -0.22 -0.61 -3.85 -1.40 -6.68 -5.82 -6.42
None 1.69 -1.81 1.75 -0.38 3.78 0.40 -2.48 -0.20 -0.48 2.10 2.28 -2.35 1.62 -1.08 4.31
Nuclear Periphery 2.76 -0.45 -4.11 -5.08 -4.12 1.70 -0.86 0.17 -2.63 1.76 2.72 -2.24 -3.49 -5.29 -2.61
Nuclear Periphery Foci 0.70 -10.06 -2.76 -3.59 8.14 2.79 -2.45 0.55 -3.19 3.91 2.99 -10.82 -1.32 -4.76 9.89
Nucleolus 4.68 8.15 -0.27 -1.52 -2.22 -0.06 2.34 2.65 1.48 2.53 -0.32 6.35 0.37 0.46 -2.08
Nucleus 5.07 -6.26 -2.14 -7.63 4.18 1.90 -3.70 1.69 -0.53 7.33 5.52 -7.52 -1.57 -8.07 6.32
Peroxisomes -5.53 6.76 0.74 4.87 -2.58 -0.76 1.50 1.46 6.33 -0.15 -5.42 5.11 1.23 5.77 -2.36
Vacuole 2.92 3.78 -25.08 -27.61 -26.36 -7.71 -15.28 -12.60 -10.18 -1.86 -2.91 -5.91 -27.99 -27.27 -17.79
Vacuole Periphery -2.03 7.01 -2.06 -1.11 -3.67 -1.49 3.96 3.90 4.99 -0.19 -3.28 8.06 -1.66 0.34 -3.67
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
Localization
Cell Percentages cytoplasm (68%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Mtr4

Mtr4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mtr4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available