Standard name
Human Ortholog
Description Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bateria

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0.05 0 0 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.43 0.21 0.48 0.41 0.66 0.64 0.62 0.57 0.54 0.61 0.59 0.59 0.77 0.82 0.88 0.53 0.49 0.54 0.27 0.06 0.34 0.65 0.59 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0.07 0.05 0 0.12 0.14 0.19 0.18 0.06 0.14 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.26 0.1 0.11 0.17 0.07 0.09 0 0 0 0 0 0 0.09 0 0 0.09 0.09 0 0.53 0.69 0.48 0.16 0.16 0.13
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.41 0.78 0.54 0.46 0.33 0.25 0.25 0.23 0.23 0.27 0.21 0.27 0.23 0.17 0.11 0.5 0.51 0.52 0.1 0.11 0.09 0.09 0.11 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 5 1 2 2 3 0 2 0 0 1 0 0 0 0 0 0 1
Bud 0 1 1 0 0 1 5 10 4 11 3 16 0 1 1 0 1 0 5 6 4 0 5 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 0 0 1 1 4 2 12 5 9 6 12 0 6 6 3 2 2 0 0 0 0 0 0
Cytoplasm 86 26 27 69 145 163 170 172 127 163 87 135 17 160 159 87 120 100 64 8 29 57 89 118
Endoplasmic Reticulum 1 0 0 0 0 1 4 5 6 4 6 9 0 0 1 9 5 4 0 1 0 0 0 1
Endosome 1 2 1 9 5 7 0 1 6 1 0 2 0 2 0 1 3 8 6 1 0 0 2 2
Golgi 0 1 0 2 0 0 1 2 1 1 0 0 0 1 1 1 5 0 0 0 0 0 0 1
Mitochondria 23 0 4 9 8 31 39 58 42 17 20 12 0 1 0 2 0 0 1 4 0 1 0 1
Nucleus 51 13 6 29 15 22 11 13 8 7 2 4 2 4 3 14 23 8 126 100 41 14 23 26
Nuclear Periphery 0 0 0 0 0 0 3 5 6 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 1 0 0 0 1 2 1 0 0 0 0 1 0 0 1 0 4 0 0 0 1
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 1 1 0 1 0 1 2 0 0 0 5 0 0 1 4 4
Vac/Vac Membrane 83 98 30 78 72 62 67 70 54 73 31 62 5 33 20 82 124 97 23 16 7 7 17 14
Unique Cell Count 200 125 56 170 220 253 272 304 235 269 147 229 22 195 181 163 244 186 239 146 87 89 150 197
Labelled Cell Count 245 142 69 198 247 291 305 354 263 290 158 257 24 211 194 199 284 220 239 146 87 89 150 197


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 21.7 9.4 8.3 8.5 9.2 14.3 16.7 18.8 26.3 26.4 35.3 6.0 14.2 23.8 12.5 11.9 11.0 7.1 7.6 8.2
Std Deviation (1e-4) 1.3 17.1 4.3 3.2 4.2 5.6 14.3 15.0 17.7 22.4 24.0 29.9 1.8 12.5 21.2 5.0 4.4 4.1 3.1 2.9 4.0
Intensity Change (Log2) -0.19 -0.15 -0.03 0.61 0.83 0.99 1.48 1.49 1.91 -0.65 0.6 1.34 0.41 0.34 0.23 -0.42 -0.31 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 2.4 2.3 2.0 1.2 0.8 1.7 1.4 1.5 2.3 5.1 6.3 0.7 0.1 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.1 1.5 2.2 2.0 0 1.3 0 0 0 0 0 0 0
Nucleus 1.1 0 -0.5 0 0 0 0 0 0 0 0 0 -0.5 -0.3 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.0 -2.9 -4.3 -4.3 -4.7 -4.5 -3.9 -4.5 -3.8 -2.5 -5.6 -6.8 -0.4 -0.4 -0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.2613 18.1068 12.7958 11.1762 13.2493 15.0169 8.5657 10.8283 8.9649 9.1566 8.3797 10.5249 8.62 9.2072 8.9662 9.4515 8.625 8.7087
Actin 0.0159 0.0007 0.0069 0.0384 0.0036 0.0006 0.0316 0.0011 0.0093 0.0028 0.009 0.0013 0.0144 0.0003 0.0025 0.0024 0.0399 0.0021
Bud 0.001 0.0008 0.0006 0.0004 0.0006 0.0002 0.0016 0.0008 0.001 0.0003 0.0013 0.0002 0.0008 0.0007 0.0011 0.0003 0.0006 0.0004
Bud Neck 0.0007 0.0003 0.0006 0.002 0.001 0.0009 0.003 0.0008 0.0009 0.0015 0.0018 0.0015 0.0011 0.0004 0.0004 0.0005 0.0011 0.0017
Bud Periphery 0.001 0.0004 0.0002 0.0004 0.0005 0.0001 0.0019 0.0005 0.0005 0.0004 0.0018 0.0002 0.0012 0.0002 0.0004 0.0004 0.0014 0.0021
Bud Site 0.0045 0.0046 0.0006 0.0007 0.0006 0.0001 0.0055 0.0027 0.0011 0.0009 0.0037 0.0004 0.0026 0.0017 0.0014 0.0007 0.0007 0.0003
Cell Periphery 0.0006 0.0001 0.0001 0.0001 0.0002 0 0.0006 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0.0002 0.0001 0.0002
Cytoplasm 0.1038 0.2149 0.125 0.0099 0.1427 0.1248 0.091 0.214 0.0807 0.1029 0.1314 0.0942 0.1371 0.2819 0.0935 0.1039 0.1135 0.1052
Cytoplasmic Foci 0.0184 0.0014 0.0125 0.0065 0.0389 0.0019 0.0225 0.0029 0.0047 0.004 0.0194 0.004 0.0194 0.0018 0.0167 0.0068 0.0014 0.0035
Eisosomes 0.0003 0 0 0.0001 0.0001 0 0.0003 0 0.0001 0.0001 0 0 0.0001 0 0 0.0001 0.0004 0
Endoplasmic Reticulum 0.0046 0.0005 0.0044 0.0005 0.0037 0.0006 0.0104 0.001 0.0012 0.004 0.0042 0.0016 0.0033 0.0015 0.0017 0.0021 0.0019 0.0036
Endosome 0.0204 0.0008 0.008 0.0129 0.0214 0.0023 0.0313 0.0019 0.0141 0.0242 0.0326 0.0127 0.0216 0.0013 0.0057 0.0213 0.0076 0.0153
Golgi 0.004 0.0003 0.0009 0.0287 0.0057 0.0004 0.006 0.0006 0.0065 0.0042 0.006 0.0038 0.0063 0 0.0039 0.0027 0.0052 0.0034
Lipid Particles 0.0103 0.0007 0.0024 0.002 0.0254 0.0016 0.0069 0.0001 0.0072 0.013 0.0145 0.006 0.018 0 0.0082 0.021 0.0023 0.0027
Mitochondria 0.0107 0.0014 0.0009 0.0065 0.0151 0.0004 0.0118 0.0018 0.0138 0.0343 0.0088 0.0045 0.0101 0.0003 0.0061 0.006 0.0059 0.0233
None 0.0033 0.0004 0.003 0.0001 0.0078 0.0005 0.0056 0.0018 0.0032 0.0008 0.0012 0.0005 0.0021 0.004 0.0035 0.0013 0.0009 0.0004
Nuclear Periphery 0.0266 0.0199 0.0129 0.0075 0.0484 0.0176 0.0277 0.0136 0.0079 0.0178 0.0201 0.0084 0.017 0.0166 0.0103 0.0097 0.0401 0.0167
Nucleolus 0.0048 0.0015 0.005 0.0003 0.0024 0.0017 0.0017 0.0008 0.0022 0.0013 0.0016 0.0014 0.0016 0.0009 0.0009 0.0013 0.0034 0.0012
Nucleus 0.7355 0.7345 0.8049 0.8762 0.64 0.8286 0.7124 0.7444 0.818 0.7566 0.6983 0.8462 0.7174 0.6786 0.8295 0.8028 0.7462 0.8039
Peroxisomes 0.0085 0.0022 0.0008 0.0054 0.0092 0.001 0.0058 0.0016 0.0077 0.0035 0.0107 0.0016 0.0031 0.0001 0.0077 0.0029 0.0006 0.0009
Punctate Nuclear 0.0141 0.0127 0.0074 0.0011 0.023 0.0156 0.0138 0.0081 0.0174 0.0226 0.0276 0.0095 0.0137 0.0077 0.0051 0.0025 0.0236 0.0065
Vacuole 0.0082 0.0014 0.0023 0.0003 0.0062 0.0008 0.0064 0.0013 0.0016 0.0032 0.0043 0.0012 0.0067 0.0015 0.0008 0.0076 0.0016 0.0034
Vacuole Periphery 0.0026 0.0005 0.0006 0.0003 0.0038 0.0003 0.0024 0.0002 0.0007 0.0016 0.0016 0.0006 0.0019 0.0003 0.0004 0.0036 0.0014 0.0032

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3624.9984 3827.0211 4829.7366 2921.0447 3694.6739 3540.1993 3595.208 3703.8799 4833.8396 3218.8028
Translational Efficiency 0.6594 0.6419 0.4934 0.9379 0.6408 0.6529 0.9341 0.9062 0.6261 0.8066

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bateria
Localization
Cell Percentages cytoplasm (65%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tdh1

Tdh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tdh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available