Standard name | |
---|---|
Human Ortholog | |
Description | Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 1 | 1 | 3 | 6 | 0 | 0 | – | 1 | 4 | 2 | 1 | 3 | 2 | 5 | 1 | 1 | 1 | 2 | 2 | 1 | 7 | 1 |
Bud | 2 | 1 | 11 | 11 | 29 | 16 | 22 | 29 | – | 36 | 29 | 38 | 3 | 6 | 3 | 6 | 6 | 2 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | – | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | – | 5 | 3 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 27 | 17 | 33 | 47 | 56 | 72 | 75 | – | 72 | 53 | 60 | 3 | 16 | 18 | 6 | 17 | 16 | 1 | 1 | 0 | 3 | 3 | 2 |
Endoplasmic Reticulum | 0 | 6 | 3 | 5 | 2 | 10 | 6 | 10 | – | 3 | 2 | 8 | 4 | 1 | 1 | 6 | 4 | 4 | 0 | 3 | 0 | 3 | 2 | 5 |
Endosome | 53 | 36 | 62 | 58 | 76 | 18 | 32 | 46 | – | 48 | 39 | 47 | 31 | 70 | 65 | 59 | 85 | 103 | 87 | 81 | 122 | 123 | 144 | 174 |
Golgi | 4 | 22 | 3 | 6 | 2 | 1 | 0 | 2 | – | 1 | 0 | 0 | 7 | 13 | 12 | 16 | 20 | 28 | 5 | 6 | 5 | 5 | 7 | 2 |
Mitochondria | 31 | 34 | 113 | 171 | 206 | 147 | 292 | 297 | – | 263 | 227 | 269 | 10 | 7 | 10 | 16 | 20 | 26 | 3 | 9 | 15 | 9 | 7 | 9 |
Nucleus | 2 | 3 | 0 | 1 | 1 | 1 | 1 | 5 | – | 3 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 3 | 15 | 5 | 0 | 1 | 1 | 0 | 2 | – | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 1 | 3 | 13 | 20 | 2 | 5 | 3 |
Nucleolus | 21 | 7 | 10 | 3 | 9 | 4 | 7 | 17 | – | 11 | 11 | 17 | 3 | 6 | 1 | 11 | 13 | 17 | 0 | 0 | 0 | 0 | 1 | 1 |
Peroxisomes | 1 | 0 | 1 | 1 | 4 | 1 | 3 | 3 | – | 10 | 4 | 5 | 0 | 4 | 3 | 3 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 6 | 0 | 21 | 11 | 25 | 20 | 34 | 27 | – | 45 | 29 | 41 | 3 | 11 | 15 | 12 | 20 | 30 | 0 | 1 | 3 | 3 | 2 | 3 |
Vac/Vac Membrane | 31 | 42 | 22 | 18 | 23 | 6 | 30 | 27 | – | 33 | 30 | 21 | 6 | 21 | 26 | 20 | 42 | 31 | 20 | 56 | 34 | 22 | 22 | 22 |
Unique Cell Count | 113 | 147 | 197 | 250 | 313 | 228 | 388 | 433 | 414 | 335 | 404 | 55 | 129 | 115 | 102 | 155 | 175 | 130 | 182 | 210 | 179 | 214 | 234 | |
Labelled Cell Count | 160 | 193 | 269 | 320 | 429 | 290 | 501 | 542 | 533 | 432 | 516 | 71 | 160 | 156 | 163 | 232 | 260 | 130 | 182 | 210 | 179 | 214 | 234 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.3 | 9.0 | 6.1 | 5.8 | 5.5 | 5.0 | 4.7 | 5.2 | – | 4.9 | 5.1 | 5.1 | 8.0 | 8.8 | 9.1 | 10.6 | 10.7 | 11.7 | 9.5 | 10.0 | 9.8 |
Std Deviation (1e-4) | 1.3 | 1.5 | 1.4 | 1.3 | 1.4 | 1.3 | 1.1 | 1.2 | – | 1.2 | 1.2 | 1.2 | 1.4 | 1.4 | 1.4 | 2.1 | 2.8 | 2.3 | 1.9 | 1.8 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.15 | -0.28 | -0.37 | -0.25 | – | -0.33 | -0.26 | -0.25 | 0.38 | 0.52 | 0.57 | 0.8 | 0.81 | 0.93 | 0.63 | 0.71 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.6 | 1.5 | 0.6 | 0 | 0.5 | – | 0 | 1.3 | 1.6 | 0 | -0.4 | -1.2 | 0.1 | -0.7 | -2.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | 2.1 | 4.3 | 3.2 | 2.9 | – | 2.9 | 2.4 | 2.1 | 0 | 1.1 | 1.9 | -0.8 | 0.7 | 0.2 |
Endoplasmic Reticulum | 0 | 0 | 1.7 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -2.0 | -1.8 | -6.2 | 0 | -6.4 | – | 0 | -5.6 | -5.9 | 3.4 | 4.1 | 4.3 | 4.4 | 4.4 | 5.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.5 | 3.5 | 4.8 | 4.3 | 5.0 |
Mitochondria | 2.4 | 1.9 | 1.5 | 0 | 2.7 | – | 0 | 2.4 | 2.2 | -5.1 | -9.5 | -8.5 | -6.9 | -8.5 | -8.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.4 | -1.3 | -1.9 | 0 | -0.7 | – | 0 | -1.0 | -0.5 | 0 | -0.2 | 0 | 1.8 | 1.2 | 1.7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -2.5 | -1.0 | -0.7 | 0 | -1.9 | – | 0 | -0.8 | -0.2 | -1.2 | -0.6 | 0.6 | 0.3 | 0.7 | 1.8 |
Vacuole | -1.5 | -1.5 | -3.5 | -1.4 | -2.1 | – | -1.3 | -0.8 | -2.7 | -0.1 | 1.3 | 2.7 | 2.0 | 3.8 | 1.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8657 | 4.1337 | 3.7021 | 3.3148 | 2.8929 | 3.7134 | 4.9494 | 6.0482 | 5.7454 | 5.4766 | 5.2738 | 5.5523 | 3.1482 | 4.1199 | 3.6142 | 3.301 | 3.0628 | 3.6607 |
Actin | 0.0016 | 0.002 | 0.0121 | 0.0001 | 0.0011 | 0.0001 | 0.019 | 0.0001 | 0.0018 | 0.0011 | 0.0005 | 0.0021 | 0.0001 | 0.0001 | 0.0001 | 0.0056 | 0.0002 | 0.004 |
Bud | 0.0002 | 0.0049 | 0.0014 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0002 |
Bud Neck | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
Bud Periphery | 0.0003 | 0.0021 | 0.0011 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0009 |
Bud Site | 0.0004 | 0.0062 | 0.0024 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0001 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 |
Cytoplasm | 0.0039 | 0.0109 | 0.0064 | 0.0054 | 0.0057 | 0.0063 | 0.004 | 0.005 | 0.0036 | 0.0058 | 0.0044 | 0.0026 | 0.0024 | 0.0091 | 0.004 | 0.0018 | 0.0054 | 0.0025 |
Cytoplasmic Foci | 0.1023 | 0.1255 | 0.0747 | 0.0762 | 0.0969 | 0.0768 | 0.0843 | 0.0559 | 0.0788 | 0.1127 | 0.0766 | 0.0514 | 0.0525 | 0.0836 | 0.0803 | 0.0205 | 0.0666 | 0.0578 |
Eisosomes | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0037 | 0.0017 | 0.0022 | 0.0007 | 0.0014 | 0.0012 | 0.0015 | 0.0017 | 0.001 | 0.0017 | 0.0014 | 0.0021 | 0.0009 | 0.0021 | 0.0012 | 0.0075 | 0.0027 | 0.0018 |
Endosome | 0.5993 | 0.6055 | 0.6998 | 0.7832 | 0.6758 | 0.7158 | 0.6693 | 0.6894 | 0.6797 | 0.6813 | 0.7309 | 0.709 | 0.6881 | 0.6875 | 0.6954 | 0.7073 | 0.6455 | 0.7163 |
Golgi | 0.047 | 0.0554 | 0.0796 | 0.0519 | 0.0931 | 0.0672 | 0.0533 | 0.0736 | 0.1023 | 0.0501 | 0.0714 | 0.1106 | 0.064 | 0.0694 | 0.0872 | 0.0739 | 0.0727 | 0.0904 |
Lipid Particles | 0.061 | 0.0262 | 0.0181 | 0.0107 | 0.0116 | 0.0228 | 0.0512 | 0.0265 | 0.0338 | 0.0373 | 0.0032 | 0.0256 | 0.0388 | 0.0294 | 0.0341 | 0.0044 | 0.0312 | 0.0198 |
Mitochondria | 0.0269 | 0.0031 | 0.0085 | 0.015 | 0.0067 | 0.0062 | 0.0033 | 0.0236 | 0.0136 | 0.0094 | 0.0547 | 0.0109 | 0.0015 | 0.0014 | 0.0047 | 0.0097 | 0.0072 | 0.0116 |
None | 0.0006 | 0.0012 | 0.0003 | 0.0002 | 0.0078 | 0.0003 | 0.0002 | 0.0001 | 0.0107 | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0008 | 0.0004 | 0.0001 | 0.0004 |
Nuclear Periphery | 0.0045 | 0.0031 | 0.0022 | 0.0015 | 0.0086 | 0.0038 | 0.0032 | 0.0068 | 0.0012 | 0.001 | 0.0009 | 0.0045 | 0.0014 | 0.004 | 0.0012 | 0.0116 | 0.0145 | 0.0044 |
Nucleolus | 0.0003 | 0.0022 | 0.0003 | 0.0001 | 0.0013 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0004 | 0.0025 | 0.0007 | 0.0002 | 0.002 | 0.0015 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0005 | 0.0003 | 0.0006 | 0.0003 | 0.0003 |
Peroxisomes | 0.0369 | 0.0105 | 0.0112 | 0.0038 | 0.0296 | 0.0053 | 0.0163 | 0.0024 | 0.0137 | 0.0081 | 0.0106 | 0.0027 | 0.0031 | 0.0041 | 0.0022 | 0.0008 | 0.0011 | 0.002 |
Punctate Nuclear | 0.0023 | 0.0013 | 0.0008 | 0.0002 | 0.004 | 0.0011 | 0.0006 | 0.0002 | 0.0004 | 0.0069 | 0.0001 | 0.0032 | 0.0001 | 0.0003 | 0.0006 | 0.0002 | 0.0003 | 0.0015 |
Vacuole | 0.0575 | 0.0875 | 0.0437 | 0.0326 | 0.0281 | 0.0417 | 0.048 | 0.0646 | 0.03 | 0.0315 | 0.0193 | 0.0307 | 0.0778 | 0.0465 | 0.0461 | 0.0603 | 0.0529 | 0.0412 |
Vacuole Periphery | 0.0508 | 0.0478 | 0.0338 | 0.0177 | 0.0253 | 0.0478 | 0.0447 | 0.0486 | 0.0263 | 0.0518 | 0.0253 | 0.0418 | 0.0686 | 0.061 | 0.0408 | 0.0928 | 0.0987 | 0.0443 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.3901 | 16.4396 | 14.7671 | 20.4349 | 10.6668 | 19.3473 | 22.3086 | 17.7139 | 19.8799 | 21.5096 |
Translational Efficiency | 1.2823 | 1.5813 | 1.4763 | 1.1016 | 2.1359 | 1.638 | 1.1895 | 1.717 | 1.4672 | 1.3686 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
440 | 2305 | 113 | 689 | 2226 | 2077 | 2006 | 1749 | 2666 | 4382 | 2119 | 2438 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 807.32 | 902.07 | 1199.29 | 1147.41 | 795.52 | 858.89 | 1061.39 | 1130.17 | 797.47 | 881.60 | 1068.74 | 1135.04 |
Standard Deviation | 107.73 | 133.27 | 164.90 | 175.73 | 103.35 | 123.60 | 140.92 | 183.31 | 104.18 | 130.57 | 145.64 | 181.37 |
Intensity Change Log 2 | — | 0.160099 | 0.570968 | 0.507168 | — | 0.110575 | 0.415985 | 0.506570 | — | 0.135732 | 0.496126 | 0.506871 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000274 | 0.000551 | 0.000342 | 0.001090 | 0.000471 | 0.000154 | 0.000050 | 0.000653 | 0.000439 | 0.000363 | 0.000065 | 0.000777 |
Bud Neck | 0.004326 | 0.000964 | 0.029521 | 0.001519 | 0.001791 | 0.001776 | 0.000917 | 0.000634 | 0.002210 | 0.001349 | 0.002442 | 0.000884 |
Bud Site | 0.008564 | 0.006341 | 0.025089 | 0.025433 | 0.007943 | 0.007614 | 0.005067 | 0.014426 | 0.008045 | 0.006944 | 0.006135 | 0.017536 |
Cell Periphery | 0.000166 | 0.000097 | 0.000152 | 0.000174 | 0.000070 | 0.000143 | 0.000041 | 0.000100 | 0.000086 | 0.000119 | 0.000047 | 0.000121 |
Cytoplasm | 0.004063 | 0.004489 | 0.006417 | 0.013499 | 0.006674 | 0.003976 | 0.003588 | 0.015710 | 0.006243 | 0.004246 | 0.003739 | 0.015085 |
Cytoplasmic Foci | 0.155802 | 0.125652 | 0.247796* | 0.118668 | 0.132359 | 0.115977 | 0.080529 | 0.117304 | 0.136228 | 0.121066 | 0.089449 | 0.117689 |
Eisosomes | 0.000044 | 0.000017 | 0.000103 | 0.000013 | 0.000019 | 0.000016 | 0.000005 | 0.000011 | 0.000023 | 0.000016 | 0.000011 | 0.000012 |
Endoplasmic Reticulum | 0.000651 | 0.000423 | 0.000410 | 0.000583 | 0.000367 | 0.000499 | 0.000433 | 0.000794 | 0.000414 | 0.000459 | 0.000432 | 0.000734 |
Endosome | 0.433262* | 0.418179* | 0.154201 | 0.257700* | 0.490711* | 0.416470* | 0.308152* | 0.262381* | 0.481230* | 0.417369* | 0.299942* | 0.261058* |
Golgi | 0.222973* | 0.282952* | 0.055623 | 0.264329* | 0.234333* | 0.227067* | 0.154066* | 0.294478* | 0.232458* | 0.256463* | 0.148816* | 0.285958* |
Lipid Particles | 0.008697 | 0.004862 | 0.010756 | 0.002850 | 0.004042 | 0.003762 | 0.001068 | 0.000629 | 0.004810 | 0.004340 | 0.001585 | 0.001257 |
Mitochondria | 0.045049 | 0.029512 | 0.008524 | 0.002293 | 0.028059 | 0.036569 | 0.006573 | 0.006664 | 0.030863 | 0.032857 | 0.006677 | 0.005429 |
Mitotic Spindle | 0.004234 | 0.004398 | 0.076832 | 0.016520 | 0.001831 | 0.004693 | 0.030504 | 0.009208 | 0.002228 | 0.004538 | 0.032974 | 0.011274 |
None | 0.000079 | 0.000457 | 0.000144 | 0.000072 | 0.000087 | 0.000120 | 0.000059 | 0.000059 | 0.000086 | 0.000297 | 0.000064 | 0.000063 |
Nuclear Periphery | 0.000145 | 0.000100 | 0.003082 | 0.000690 | 0.000049 | 0.000159 | 0.001345 | 0.000140 | 0.000065 | 0.000128 | 0.001437 | 0.000295 |
Nuclear Periphery Foci | 0.003620 | 0.000849 | 0.012832 | 0.004776 | 0.000786 | 0.001532 | 0.007146 | 0.000659 | 0.001254 | 0.001172 | 0.007449 | 0.001823 |
Nucleolus | 0.000182 | 0.000061 | 0.010770 | 0.000060 | 0.000107 | 0.000054 | 0.000477 | 0.000051 | 0.000120 | 0.000058 | 0.001025 | 0.000054 |
Nucleus | 0.001981 | 0.000071 | 0.002522 | 0.000082 | 0.000137 | 0.000078 | 0.000282 | 0.000069 | 0.000442 | 0.000075 | 0.000401 | 0.000073 |
Peroxisomes | 0.015497 | 0.003034 | 0.032823 | 0.002147 | 0.003149 | 0.004839 | 0.001328 | 0.000997 | 0.005187 | 0.003890 | 0.003008 | 0.001322 |
Vacuole | 0.035777 | 0.047208 | 0.184033 | 0.201693* | 0.036354 | 0.064285 | 0.245192* | 0.161924 | 0.036258 | 0.055302 | 0.241930* | 0.173163 |
Vacuole Periphery | 0.054614 | 0.069783 | 0.138027* | 0.085809 | 0.050659 | 0.110217* | 0.153179* | 0.113110* | 0.051312 | 0.088948 | 0.152371* | 0.105395* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.73 | -0.86 | -1.92 | -0.94 | -1.73 | 1.06 | 1.42 | -0.34 | -1.11 | -1.35 | 0.23 | 1.50 | -0.80 | -1.03 | -2.08 |
Bud Neck | 2.91 | -4.80 | 2.37 | -2.35 | 5.42 | 0.03 | 3.29 | 4.51 | 2.55 | 2.81 | 2.43 | -0.77 | 4.52 | 1.88 | 4.86 |
Bud Site | 1.22 | -3.37 | -5.13 | -6.68 | 0.05 | 0.28 | 2.81 | -4.33 | -4.64 | -6.78 | 1.25 | 1.85 | -6.73 | -8.26 | -8.43 |
Cell Periphery | 2.42 | 0.34 | -0.25 | -3.67 | -0.75 | -2.36 | 6.22 | -3.19 | 1.36 | -6.91 | -2.01 | 6.34 | -3.54 | -0.12 | -8.89 |
Cytoplasm | -0.56 | -1.00 | -5.01 | -4.96 | -1.77 | 2.94 | 2.87 | -5.53 | -7.60 | -7.56 | 2.55 | 2.44 | -6.75 | -8.89 | -8.68 |
Cytoplasmic Foci | 4.46 | -3.57 | 4.73 | 1.28 | 5.04 | 4.27 | 14.86 | 3.93 | -0.32 | -10.63 | 4.78 | 12.96 | 5.38 | 1.15 | -8.00 |
Eisosomes | 3.64 | -1.97 | 4.11 | 1.95 | 3.04 | 2.06 | 11.37 | 3.81 | 2.30 | -3.18 | 3.95 | 5.42 | 5.62 | 3.05 | -0.42 |
Endoplasmic Reticulum | 1.78 | 1.42 | 0.46 | -2.06 | -1.35 | -1.84 | -1.11 | -1.64 | -1.11 | -1.35 | -0.86 | -0.41 | -1.70 | -1.46 | -1.54 |
Endosome | 1.08 | 14.38 | 11.74 | 17.05 | -6.39 | 8.89 | 23.05 | 29.85 | 20.09 | 6.20 | 9.47 | 24.40 | 32.58 | 27.02 | 5.76 |
Golgi | -5.45 | 12.92 | -2.84 | 1.89 | -15.95 | 1.10 | 12.81 | -7.64 | -8.49 | -18.54* | -4.37 | 14.35 | -7.78 | -4.50 | -20.46 |
Lipid Particles | 1.80 | -0.49 | 2.81 | 2.06 | 2.26 | 0.26 | 4.62 | 5.49 | 4.79 | 2.69 | 0.54 | 4.90 | 5.55 | 5.82 | 1.09 |
Mitochondria | 2.75 | 5.88 | 7.87 | 15.85 | 1.97 | -2.99 | 11.47 | 11.33 | 12.62 | -0.10 | -0.91 | 13.16 | 14.17 | 18.55 | 1.42 |
Mitotic Spindle | -0.19 | -4.29 | -4.26 | -4.47 | 3.50 | -3.08 | -11.05 | -5.96 | -3.17 | 7.62 | -3.52 | -11.71 | -7.59 | -5.48 | 7.66 |
None | -1.14 | -2.21 | 0.29 | 1.16 | 2.01 | -1.05 | 1.52 | 1.55 | 2.26 | 0.17 | -1.20 | 1.38 | 1.41 | 1.33 | 0.27 |
Nuclear Periphery | 0.82 | -4.82 | -2.18 | -2.40 | 3.63 | -3.32 | -9.42 | -4.74 | 0.48 | 8.72 | -3.22 | -10.18 | -3.24 | -2.32 | 7.60 |
Nuclear Periphery Foci | 1.67 | -1.97 | -0.59 | -3.02 | 1.76 | -1.25 | -5.89 | 0.47 | 1.57 | 6.14 | 0.11 | -5.77 | -1.15 | -1.35 | 5.18 |
Nucleolus | 1.45 | -2.98 | 1.51 | 0.09 | 3.00 | 1.50 | -2.19 | 1.62 | 0.22 | 2.54 | 1.82 | -3.59 | 2.07 | 0.39 | 3.84 |
Nucleus | 1.08 | -0.47 | 1.07 | -0.82 | 1.89 | 1.29 | -2.28 | 1.52 | 0.41 | 3.63 | 1.25 | -0.10 | 1.25 | -0.00 | 3.67 |
Peroxisomes | 4.44 | -2.90 | 4.75 | 1.89 | 5.50 | -1.91 | 4.35 | 5.69 | 4.73 | 1.45 | 1.85 | 3.07 | 6.75 | 5.94 | 4.20 |
Vacuole | -2.68 | -7.93 | -19.45 | -19.88 | -1.14 | -11.37 | -44.60 | -27.86 | -20.78 | 13.46 | -10.10 | -45.22 | -34.25 | -29.69 | 11.87 |
Vacuole Periphery | -2.51 | -3.91 | -4.32 | -2.89 | 2.42 | -13.81 | -23.10 | -14.98 | -0.53 | 7.59 | -12.28 | -23.39 | -15.32 | -4.41 | 9.79 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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