Standard name
Human Ortholog
Description Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.96 0.96 – 0.98 0.9 0.88 0.88 0.67 0.79 0.55 0.72 0.99 0.99 0.99 0.94 0.97 0.97 0.7 0.78 0.52 0.87 0.84 0.85
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0.06 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0.05 0.09 0.1 0.37 0.22 0.52 0.24 0 0 0 0 0 0 0.15 0.1 0.23 0 0.05 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 – 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 1 0 0 1 1 1 1 0 0 0 0 0 1 5 4 2 3 6 7
Bud 3 0 0 – 0 2 1 2 3 2 4 3 0 0 0 0 0 0 1 1 1 1 11 10
Bud Neck 0 2 1 – 0 3 2 4 0 0 2 2 3 1 0 5 2 0 1 3 1 0 1 3
Bud Site 1 0 0 – 0 0 0 0 0 0 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 2 1 – 1 5 2 1 0 1 0 4 2 1 5 2 1 3 2 3 1 0 3 5
Cytoplasm 362 321 80 – 114 178 248 221 66 140 96 179 365 478 623 335 253 223 283 271 53 149 405 442
Endoplasmic Reticulum 2 10 1 – 0 7 6 3 5 2 3 6 2 1 0 21 18 16 13 7 2 1 2 2
Endosome 1 0 0 – 1 0 5 1 0 0 0 5 1 2 1 6 0 1 5 1 2 1 4 4
Golgi 1 0 0 – 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 1 0 0 2 1
Mitochondria 17 3 0 – 0 9 25 24 36 39 91 60 0 0 0 13 3 2 61 35 23 7 23 19
Nucleus 0 1 1 – 0 1 2 2 2 2 1 2 1 1 1 1 1 1 0 0 0 0 3 0
Nuclear Periphery 1 2 0 – 0 0 0 1 0 2 0 0 0 0 0 1 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 1 0 0 0 1 1 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
SpindlePole 2 0 1 – 1 1 4 2 0 0 6 6 1 1 1 1 1 0 1 4 0 0 5 8
Vac/Vac Membrane 5 4 0 – 1 1 5 7 1 10 3 7 0 0 3 11 6 3 17 6 6 2 1 8
Unique Cell Count 380 334 83 116 197 283 251 98 177 174 249 370 485 629 358 262 229 406 347 102 172 485 522
Labelled Cell Count 400 345 85 118 208 300 268 114 200 209 275 375 486 636 400 286 250 406 347 102 172 485 522


(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.2 5.3 – 5.8 4.6 5.0 5.2 3.7 4.5 3.8 4.2 5.3 5.3 5.5 5.7 5.6 5.0 5.3 5.1 5.5
Std Deviation (1e-4) 0.7 0.7 1.7 – 1.6 1.0 1.8 1.5 0.9 1.4 0.9 1.1 1.2 1.3 1.5 1.1 1.8 1.0 1.6 1.4 1.6
Intensity Change (Log2) – – – – 0.13 -0.21 -0.09 -0.04 -0.5 -0.22 -0.47 -0.33 0.01 0.01 0.06 0.1 0.08 -0.07 -0.01 -0.04 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.27 -0.2808 -0.2057 -0.1874 0.0728 -0.3839 -0.6591 -0.4987 -0.3714 -0.7108 -0.4833 -0.5075 -1.1643 -0.7281 -1.0387 -0.8984 -0.8518 -0.7486
Actin 0.01 0.0006 0.0246 0.0182 0.0523 0.0004 0.0312 0.0003 0.0028 0.0026 0.0039 0.0018 0.0331 0.0009 0.0097 0.0003 0.0159 0.0146
Bud 0.002 0.0012 0.0034 0.0004 0.001 0.0008 0.0066 0.0138 0.0066 0.0046 0.0005 0.0005 0.0007 0.0067 0.0016 0.0001 0.0001 0.0003
Bud Neck 0.011 0.0002 0.0044 0.0009 0.001 0.0009 0.0081 0.0001 0.0004 0.0023 0.0011 0.0002 0.0026 0.0001 0.0003 0.0001 0.0003 0.0163
Bud Periphery 0.0056 0.0038 0.0056 0.0009 0.0015 0.0013 0.0053 0.0019 0.0074 0.0019 0.0014 0.0004 0.0023 0.0056 0.0026 0.0004 0.0003 0.0005
Bud Site 0.0074 0.0071 0.0158 0.0002 0.0091 0.0001 0.0164 0.0095 0.0125 0.0041 0.0007 0.0004 0.0015 0.0041 0.0024 0 0.0003 0.0008
Cell Periphery 0.0014 0.0003 0.001 0.0001 0.0004 0.0001 0.0005 0.0003 0.0003 0.0005 0.0002 0.0001 0.0003 0.0003 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0329 0.0338 0.0392 0.0473 0.0304 0.0857 0.0196 0.0712 0.0278 0.0343 0.0671 0.0578 0.0832 0.1721 0.1608 0.2105 0.1748 0.2129
Cytoplasmic Foci 0.0203 0.0073 0.0226 0.011 0.0287 0.0229 0.0178 0.0045 0.0219 0.0324 0.0038 0.0049 0.0183 0.0054 0.0163 0.0026 0.0134 0.0131
Eisosomes 0.0006 0.0003 0.0005 0.0002 0.0008 0.0002 0.0023 0.0002 0.0003 0.0002 0.0001 0.0001 0.0007 0.0003 0.0003 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0099 0.0017 0.0029 0.0018 0.0027 0.0009 0.0015 0.0017 0.001 0.0166 0.0016 0.0007 0.0056 0.003 0.0052 0.0018 0.0048 0.004
Endosome 0.0179 0.0013 0.0117 0.0131 0.0585 0.0037 0.0179 0.002 0.0107 0.0131 0.0232 0.0024 0.0199 0.0019 0.0145 0.0007 0.0089 0.0074
Golgi 0.0068 0.0003 0.0069 0.0199 0.0214 0.0005 0.0089 0.0004 0.0058 0.0081 0.0078 0.0038 0.0057 0.0002 0.002 0.0001 0.0055 0.0014
Lipid Particles 0.0153 0.0008 0.0097 0.0032 0.0411 0.0013 0.0195 0.0007 0.0092 0.0051 0.0057 0.0013 0.007 0.0009 0.0073 0.0002 0.0148 0.0041
Mitochondria 0.0166 0.0011 0.0114 0.0445 0.0447 0.0006 0.007 0.0003 0.0131 0.0035 0.0047 0.008 0.0017 0.0005 0.0067 0.0003 0.001 0.0052
None 0.8187 0.9338 0.8121 0.8306 0.6598 0.8691 0.8074 0.8885 0.8646 0.8522 0.8699 0.9053 0.7912 0.7935 0.7521 0.7802 0.7432 0.7036
Nuclear Periphery 0.0034 0.0005 0.0025 0.0011 0.0016 0.0015 0.0028 0.0004 0.0004 0.0076 0.0011 0.0008 0.0094 0.0008 0.0023 0.0007 0.0044 0.0054
Nucleolus 0.0007 0.0005 0.0011 0.0002 0.0013 0.0003 0.0015 0.0004 0.0006 0.0008 0.0003 0.0037 0.0003 0.0003 0.0005 0.0001 0.0003 0.0008
Nucleus 0.0015 0.0008 0.0014 0.0006 0.0011 0.0043 0.0037 0.0015 0.0019 0.0018 0.0005 0.0018 0.0019 0.0011 0.0017 0.0006 0.0013 0.0022
Peroxisomes 0.0122 0.0013 0.0097 0.0026 0.026 0.0011 0.0129 0.0001 0.008 0.0033 0.0008 0.0004 0.0088 0.0002 0.0061 0.0001 0.0027 0.0032
Punctate Nuclear 0.0018 0.0021 0.0033 0.0008 0.0016 0.0035 0.0071 0.0017 0.0038 0.0015 0.0009 0.0053 0.0038 0.0011 0.003 0.0004 0.005 0.0025
Vacuole 0.0031 0.0011 0.0092 0.0016 0.013 0.0006 0.0011 0.0005 0.0008 0.0026 0.0033 0.0003 0.0013 0.0011 0.0031 0.0004 0.0022 0.0011
Vacuole Periphery 0.0009 0.0002 0.0013 0.0007 0.0021 0.0001 0.0007 0.0001 0.0003 0.0009 0.0014 0.0002 0.0008 0.0001 0.0009 0.0001 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.4048 5.9064 6.209 6.4307 1.0708 7.1539 4.6034 6.8858 6.9256 6.3229
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2290 1332 878 1469 1321 1546 321 2511 3611 2878 1199 3980

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 609.03 677.86 882.16 862.57 676.47 692.93 903.85 876.35 633.70 685.96 887.97 871.26
Standard Deviation 62.11 101.75 95.85 124.51 73.20 87.23 100.34 115.64 73.91 94.53 97.55 119.18
Intensity Change Log 2 — 0.154474 0.534527 0.502128 — 0.034684 0.418057 0.373481 — 0.092678 0.474411 0.435862

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000139 0.000130 0.000329 0.001998 0.000121 0.000242 0.000178 0.000776 0.000132 0.000190 0.000289 0.001227
Bud Neck 0.026354 0.012264 0.005301 0.013741 0.015159 0.042129 0.002323 0.015728 0.022259 0.028307 0.004504 0.014995
Bud Site 0.005694 0.005429 0.009470 0.051551 0.003755 0.013481 0.002889 0.039088 0.004984 0.009755 0.007708 0.043688
Cell Periphery 0.000185 0.000115 0.000275 0.000678 0.000176 0.000171 0.000256 0.000400 0.000182 0.000145 0.000270 0.000503
Cytoplasm 0.473332* 0.493731* 0.303745* 0.428792* 0.451842* 0.387803* 0.426079* 0.504341* 0.465470* 0.436829* 0.336497* 0.476457*
Cytoplasmic Foci 0.221109* 0.157962 0.021372 0.046481 0.275097* 0.243919* 0.050359 0.021880 0.240859* 0.204136* 0.029133 0.030960
Eisosomes 0.000371 0.000079 0.000072 0.000188 0.000253 0.000193 0.000118 0.000065 0.000328 0.000141 0.000084 0.000110
Endoplasmic Reticulum 0.001072 0.001235 0.006911 0.008489 0.000990 0.001605 0.004120 0.002332 0.001042 0.001434 0.006164 0.004605
Endosome 0.009149 0.009552 0.004566 0.008824 0.011661 0.024236 0.007811 0.003866 0.010068 0.017440 0.005434 0.005696
Golgi 0.004052 0.000929 0.000173 0.004198 0.003533 0.004897 0.000121 0.001006 0.003862 0.003060 0.000159 0.002184
Lipid Particles 0.011754 0.007952 0.003857 0.004404 0.014897 0.011913 0.005554 0.001525 0.012904 0.010080 0.004311 0.002588
Mitochondria 0.007152 0.005695 0.000839 0.002851 0.004584 0.014150 0.002764 0.003050 0.006213 0.010236 0.001355 0.002977
Mitotic Spindle 0.000510 0.001902 0.005351 0.017288 0.000606 0.007072 0.000721 0.023172 0.000545 0.004679 0.004111 0.021000
None 0.005900 0.008141 0.003972 0.003164 0.003337 0.002757 0.002613 0.003969 0.004962 0.005249 0.003608 0.003672
Nuclear Periphery 0.000394 0.000935 0.003589 0.001832 0.000461 0.000863 0.001987 0.001369 0.000418 0.000896 0.003160 0.001540
Nuclear Periphery Foci 0.000374 0.000768 0.003511 0.005280 0.000380 0.000329 0.005182 0.001508 0.000376 0.000532 0.003958 0.002900
Nucleolus 0.001704 0.002601 0.001126 0.000540 0.001131 0.001714 0.000489 0.000429 0.001495 0.002124 0.000955 0.000470
Nucleus 0.178910* 0.235290* 0.548609* 0.207894* 0.163977* 0.139576* 0.309259* 0.290673* 0.173447* 0.183874* 0.484529* 0.260120*
Peroxisomes 0.004818 0.004423 0.000543 0.001827 0.007297 0.014101 0.000503 0.001420 0.005725 0.009622 0.000532 0.001570
Vacuole 0.046012 0.049348 0.075641 0.186563 0.039677 0.085745 0.176484 0.082273 0.043695 0.068899 0.102639 0.120766
Vacuole Periphery 0.001014 0.001516 0.000749 0.003418 0.001066 0.003105 0.000189 0.001129 0.001033 0.002370 0.000599 0.001974

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.28 -6.84 -5.47 -5.49 -4.39 -3.27 -2.69 -3.07 -2.56 -2.34 -2.84 -7.33 -5.60 -5.26 -4.15
Bud Neck 7.24 12.88 8.39 0.10 -4.08 -9.68 8.59 0.69 10.65 -10.10 -3.30 14.65 7.38 8.95 -8.21
Bud Site 0.38 -1.75 -9.52 -9.45 -7.62 -6.21 0.05 -12.46 -7.27 -12.41 -4.91 -1.84 -15.54 -12.09 -11.92
Cell Periphery 7.56 -4.76 -7.95 -9.53 -5.36 0.56 -4.88 -5.28 -5.33 -1.61 4.85 -6.29 -9.05 -10.39 -4.50
Cytoplasm -1.65 21.14 11.90 11.42 -9.04 6.66 5.51 -1.00 -8.32 -6.25 4.83 20.48 6.46 1.04 -15.43
Cytoplasmic Foci 9.72 46.43 40.76 22.96 -8.63 3.79 33.75 43.03 38.65 7.20 6.99 58.11 60.84 44.34 -0.49
Eisosomes 7.99 7.89 5.21 -7.33 -6.92 3.76 7.16 12.88 14.67 8.01 7.81 9.64 9.23 5.12 -1.25
Endoplasmic Reticulum -1.15 -14.10 -12.89 -12.46 0.39 -1.37 -7.58 -7.00 -3.28 4.42 -1.68 -15.73 -15.43 -11.24 5.99
Endosome -0.49 6.24 3.32 3.28 -1.33 -7.52 3.57 10.00 13.92 6.21 -7.48 7.65 10.32 14.00 3.44
Golgi 7.77 9.89 2.40 -1.86 -2.98 -2.03 8.15 5.96 7.62 -2.50 2.10 12.67 6.39 4.50 -3.76
Lipid Particles 4.03 10.66 11.12 4.79 -0.62 2.14 7.62 12.81 10.14 5.44 3.45 13.33 18.40 11.53 5.30
Mitochondria 1.31 8.80 5.57 3.14 -1.25 -5.93 1.53 2.53 7.69 -0.31 -3.74 7.48 6.23 8.06 -1.71
Mitotic Spindle -2.26 -2.78 -6.29 -5.30 -3.38 -4.30 0.30 -9.17 -5.28 -9.30 -4.97 -2.67 -11.20 -7.53 -7.65
None -1.65 2.86 4.09 5.25 1.91 0.93 2.07 -0.21 -1.58 -3.26 -0.25 3.14 3.30 3.76 -0.02
Nuclear Periphery -4.16 -17.06 -10.38 -6.66 5.72 -2.29 -11.73 -14.00 -5.93 1.11 -4.27 -19.23 -17.04 -8.96 7.53
Nuclear Periphery Foci -4.45 -8.91 -10.14 -9.31 -2.94 0.83 -8.41 -5.95 -6.02 5.99 -2.46 -11.92 -11.57 -10.81 2.78
Nucleolus -2.18 1.68 5.95 5.61 3.07 -2.82 4.92 5.27 9.44 0.43 -2.70 2.36 7.91 8.82 3.33
Nucleus -8.69 -36.77 -4.72 3.75 31.37 3.92 -9.48 -20.04 -24.49 -0.52 -2.54 -34.73 -20.15 -15.60 22.92
Peroxisomes 0.45 10.62 7.92 5.70 -2.60 -4.96 8.49 7.42 11.37 -2.27 -5.19 13.32 10.84 12.58 -3.08
Vacuole -1.72 -10.56 -31.66 -30.02 -20.35 -12.63 -13.99 -21.15 -7.93 8.04 -10.72 -16.64 -35.36 -26.11 -6.06
Vacuole Periphery -2.05 1.45 -1.41 -0.58 -1.90 -5.22 6.87 1.16 5.54 -3.27 -5.65 2.75 -0.59 3.55 -2.22
Standard name
Human Ortholog
Description Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p
Localization
Cell Percentages cytoplasm (34%), ER (1%), mixed (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Iks1

Iks1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Iks1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available