Standard name
Human Ortholog
Description Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.2 0.33 0.25 0.24 0.15
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0.06 0.08
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.07 0.1 0.09 0.12 0 0 0 0 0 0 0 0 0.17 0.2 0.12 0 0.07 0.1 0.15 0.24 0.28 0.21 0.25 0.23
Cytoplasm 0.92 0.94 0.9 0.91 0.91 0.85 0.74 0.75 0.67 0.7 0.51 0.58 0.98 0.97 0.99 0.95 0.95 0.95 0.28 0.32 0.11 0.29 0.28 0.31
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.12 0.09 0.1 0 0 0.05 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0.11 0.14 0.35 0.42 0.32 0.53 0.5 0.64 0.65 0 0 0 0.05 0 0 0.12 0.06 0.1 0.07 0.06 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 11 14 3 0 0 0 0 0 0 0 0 1 4 1 0 1 1 31 79 108 29 70 39
Bud 1 2 7 5 6 10 3 7 15 19 2 9 1 1 1 0 2 0 4 4 3 0 5 7
Bud Neck 2 7 4 1 2 0 1 1 0 2 0 0 1 2 2 2 1 1 13 29 14 3 17 20
Bud Site 0 0 0 2 1 1 0 0 2 5 1 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 14 37 27 36 11 9 10 4 7 9 1 1 29 61 31 16 18 16 30 93 93 25 72 60
Cytoplasm 177 346 282 273 338 346 373 273 337 437 57 148 162 303 255 371 252 146 58 126 35 34 83 82
Endoplasmic Reticulum 0 7 4 4 0 1 4 1 5 0 1 1 0 1 0 55 33 14 21 12 12 6 11 5
Endosome 1 0 0 0 2 0 2 1 0 1 1 0 0 1 0 5 0 0 4 2 6 0 4 5
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 3 2 1 0 1 0
Mitochondria 20 2 10 32 52 141 212 115 268 317 71 167 2 1 1 18 11 3 25 24 33 7 16 24
Nucleus 1 0 0 0 0 1 1 1 5 4 1 2 1 0 2 1 1 0 0 0 0 1 0 0
Nuclear Periphery 0 2 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0
Nucleolus 3 0 1 1 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 1 2 3 0 1 0 0 1 0 0 0 1 0 3 0 1 0 0 2 0 0 0
SpindlePole 1 2 6 7 4 1 8 7 2 12 0 6 1 0 0 5 1 1 0 0 1 0 0 1
Vac/Vac Membrane 0 0 0 0 1 1 2 3 3 4 0 2 0 0 0 2 5 1 6 11 5 4 2 8
Unique Cell Count 193 369 312 299 371 408 505 363 504 628 111 257 166 312 258 390 265 154 206 396 327 118 292 263
Labelled Cell Count 222 418 356 367 420 511 619 414 644 811 136 340 199 375 293 478 327 184 206 396 327 118 292 263


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.6 4.3 3.9 4.1 3.4 3.0 3.4 3.0 2.8 3.2 2.8 4.9 4.6 4.9 5.6 6.2 6.3 4.5 4.5 5.4
Std Deviation (1e-4) 1.6 0.7 1.3 1.1 1.7 1.3 1.1 1.4 1.0 0.9 1.4 1.0 1.3 1.3 1.6 1.9 2.0 2.0 1.2 1.1 1.7
Intensity Change (Log2) – – – -0.11 -0.07 -0.31 -0.5 -0.32 -0.49 -0.59 -0.4 -0.6 0.19 0.12 0.2 0.4 0.54 0.56 0.08 0.07 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0304 0.1641 0.5364 0.6806 1.0464 0.4297 1.0963 1.3345 1.2627 0.8258 1.0776 0.9505 4.014 2.0066 2.4639 4.5251 2.9671 2.1485
Actin 0.0247 0.0021 0.0111 0.0015 0.0101 0.0039 0.0095 0.0035 0.008 0.0053 0.001 0.0106 0.0468 0.0014 0.0077 0.0002 0.0192 0.0072
Bud 0.0012 0.0001 0.0005 0.0003 0.0036 0.0004 0.0014 0.0002 0.0015 0.0021 0.0001 0.0001 0.0004 0.0005 0.0044 0 0.0002 0.0007
Bud Neck 0.0129 0.0023 0.001 0.0022 0.0108 0.0029 0.0024 0.0023 0.0015 0.0012 0.0002 0.0019 0.0018 0.0011 0.005 0.0001 0.0005 0.001
Bud Periphery 0.0044 0.0004 0.0028 0.0013 0.0065 0.0031 0.0069 0.0005 0.0048 0.0064 0.0001 0.0003 0.0004 0.0019 0.0059 0.0001 0.0003 0.005
Bud Site 0.0413 0.0099 0.0024 0.0007 0.0335 0.0008 0.0071 0.0466 0.009 0.0021 0.0002 0.0002 0.0056 0.0091 0.0208 0 0.0009 0.0002
Cell Periphery 0.0443 0.0078 0.0099 0.0053 0.0024 0.0031 0.0101 0.0088 0.0072 0.0037 0.0008 0.001 0.0025 0.0112 0.007 0.0005 0.0018 0.0026
Cytoplasm 0.1878 0.3242 0.1834 0.3608 0.1893 0.2546 0.0913 0.3268 0.2301 0.486 0.2515 0.228 0.1286 0.1833 0.3998 0.0359 0.2425 0.205
Cytoplasmic Foci 0.0093 0.0098 0.0095 0.0197 0.0475 0.0078 0.0264 0.0051 0.0042 0.008 0.0598 0.0126 0.2134 0.0032 0.0071 0.0019 0.0155 0.003
Eisosomes 0.0034 0.0033 0.0019 0.0006 0.0017 0.0008 0.0014 0.0009 0.002 0.0015 0.0001 0.0005 0.0006 0.0013 0.0006 0.0004 0.0003 0.0006
Endoplasmic Reticulum 0.0408 0.0059 0.0028 0.0154 0.0045 0.0014 0.0016 0.0039 0.02 0.0047 0.0013 0.0015 0.0088 0.0045 0.0251 0.0008 0.0049 0.0028
Endosome 0.021 0.0008 0.0022 0.0051 0.0549 0.0009 0.0198 0.0004 0.0037 0.0267 0.0019 0.0004 0.2143 0.0003 0.0108 0.0001 0.0094 0.0035
Golgi 0.0027 0.0006 0.0016 0.0012 0.0208 0.0012 0.0134 0.0005 0.0008 0.0108 0.0008 0.0002 0.0144 0.0002 0.0019 0 0.0021 0.0027
Lipid Particles 0.0055 0.0014 0.0017 0.0087 0.0632 0.0008 0.0314 0.0005 0.0011 0.0038 0.0143 0.0004 0.0324 0.001 0.0009 0.0003 0.0017 0.0017
Mitochondria 0.0062 0.0002 0.0073 0.0014 0.1433 0.0188 0.0031 0.0001 0.0006 0.016 0.0002 0.0001 0.0024 0.0003 0.001 0.0001 0.0008 0.0105
None 0.478 0.6278 0.7585 0.5689 0.3598 0.6956 0.73 0.5983 0.697 0.404 0.6454 0.7409 0.1785 0.7776 0.4931 0.9588 0.6839 0.7511
Nuclear Periphery 0.0729 0.0003 0.0003 0.0004 0.0013 0.0001 0.0003 0.0002 0.0051 0.0007 0.0003 0.0001 0.0098 0.0003 0.0043 0.0001 0.0031 0.0002
Nucleolus 0.0022 0.0004 0.0003 0.0003 0.0022 0.0002 0.0004 0.0001 0.0003 0.0003 0.0003 0.0001 0.0009 0.0005 0.0004 0.0001 0.0002 0.0001
Nucleus 0.0166 0.0007 0.0004 0.0005 0.0017 0.0003 0.0004 0.0003 0.0012 0.001 0.0003 0.0002 0.0025 0.0008 0.0017 0.0001 0.0025 0.0002
Peroxisomes 0.0009 0.0002 0.0008 0.0005 0.0326 0.0024 0.0397 0.0002 0.0003 0.0009 0.0091 0.0003 0.0746 0.0003 0.0003 0.0001 0.0028 0.0009
Punctate Nuclear 0.011 0.0007 0.0005 0.0007 0.0011 0.0003 0.0009 0.0002 0.0006 0.0004 0.0116 0.0004 0.0397 0.0004 0.0011 0.0002 0.0069 0.0001
Vacuole 0.0073 0.001 0.0011 0.0041 0.007 0.0005 0.0023 0.0004 0.0009 0.0128 0.0005 0.0002 0.0161 0.0009 0.0011 0.0004 0.0005 0.0006
Vacuole Periphery 0.0058 0 0.0002 0.0003 0.0021 0.0001 0.0002 0 0.0002 0.0016 0 0 0.0054 0 0.0003 0 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.7469 3.022 2.2424 5.8009 3.9447 4.0544 3.8194 7.4242 4.5213 5.8231
Translational Efficiency 0.6833 1.032 2.051 0.4482 0.7752 1.0716 1.1298 0.5476 0.8365 0.888

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1827 1504 114 2796 2144 2110 149 350 3971 3614 263 3146

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 645.06 692.31 887.54 745.25 691.67 711.90 900.49 942.32 670.23 703.75 894.88 767.17
Standard Deviation 71.77 94.18 104.95 84.94 74.04 98.27 89.14 135.29 76.61 97.07 96.53 110.85
Intensity Change Log 2 — 0.101985 0.460379 0.208291 — 0.041591 0.380626 0.446133 — 0.071051 0.419663 0.336239

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002279 0.004509 0.000568 0.000229 0.001899 0.003263 0.000205 0.000064 0.002074 0.003782 0.000363 0.000211
Bud Neck 0.032192 0.040697 0.003100 0.012480 0.029276 0.063509 0.001388 0.015404 0.030618 0.054015 0.002130 0.012805
Bud Site 0.008270 0.020767 0.003245 0.020431 0.007003 0.024373 0.002192 0.012035 0.007586 0.022872 0.002649 0.019496
Cell Periphery 0.001493 0.001029 0.000811 0.000481 0.002081 0.001397 0.000416 0.000366 0.001810 0.001244 0.000587 0.000468
Cytoplasm 0.492942* 0.377350* 0.227950* 0.538964* 0.520523* 0.460505* 0.295109* 0.640068* 0.507833* 0.425900* 0.265998* 0.550212*
Cytoplasmic Foci 0.191484* 0.280314* 0.078605 0.032141 0.134021 0.196995* 0.061933 0.013674 0.160459 0.231669* 0.069160 0.030087
Eisosomes 0.007851 0.004107 0.000300 0.000113 0.008405 0.004955 0.000194 0.000056 0.008150 0.004602 0.000240 0.000107
Endoplasmic Reticulum 0.001656 0.003246 0.006357 0.000977 0.001509 0.005353 0.002544 0.000766 0.001576 0.004476 0.004197 0.000954
Endosome 0.009496 0.024231 0.008057 0.002198 0.004359 0.014002 0.005359 0.000966 0.006723 0.018259 0.006528 0.002061
Golgi 0.011413 0.020630 0.000204 0.000274 0.007920 0.013239 0.000119 0.000083 0.009527 0.016315 0.000156 0.000252
Lipid Particles 0.027065 0.030137 0.009046 0.001210 0.014099 0.016577 0.005622 0.000796 0.020064 0.022220 0.007106 0.001164
Mitochondria 0.006579 0.010629 0.000239 0.000564 0.003856 0.009956 0.000196 0.001051 0.005109 0.010236 0.000215 0.000618
Mitotic Spindle 0.000390 0.000982 0.000634 0.004398 0.000455 0.001730 0.004823 0.001609 0.000425 0.001419 0.003007 0.004088
None 0.005232 0.002062 0.002111 0.002015 0.002601 0.001418 0.002144 0.004298 0.003812 0.001686 0.002130 0.002269
Nuclear Periphery 0.000158 0.000324 0.001356 0.000515 0.000174 0.000178 0.001209 0.000470 0.000167 0.000239 0.001273 0.000510
Nuclear Periphery Foci 0.000341 0.000611 0.005754 0.000282 0.000247 0.000338 0.001381 0.000128 0.000290 0.000452 0.003276 0.000265
Nucleolus 0.000614 0.000936 0.001057 0.000279 0.000380 0.000687 0.000845 0.000444 0.000488 0.000791 0.000937 0.000297
Nucleus 0.148875* 0.096301 0.505641* 0.322379* 0.223867* 0.112943 0.472827* 0.258703* 0.189364* 0.106017 0.487051* 0.315295*
Peroxisomes 0.011308 0.023334 0.000976 0.000748 0.008225 0.017813 0.000800 0.000186 0.009644 0.020111 0.000876 0.000685
Vacuole 0.039757 0.056323 0.143786 0.059052 0.028744 0.049745 0.140488 0.048670 0.033811 0.052482 0.141918 0.057897
Vacuole Periphery 0.000603 0.001482 0.000202 0.000273 0.000357 0.001025 0.000205 0.000164 0.000470 0.001215 0.000204 0.000260

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.72 4.23 6.50 8.37 2.91 -3.31 5.20 5.84 11.80 2.98 -4.94 6.77 8.27 13.35 2.79
Bud Neck -3.00 15.31 10.14 11.72 -7.40 -10.98 18.33 5.14 13.24 -4.95 -10.76 23.85 12.60 20.22 -10.82
Bud Site -7.53 4.07 -6.10 0.50 -8.43 -10.52 3.71 -1.35 3.85 -2.67 -12.89 5.37 -6.81 2.24 -9.17
Cell Periphery 3.58 4.84 12.84 5.15 3.50 3.54 9.11 8.25 8.00 0.85 4.74 10.42 12.71 11.97 2.63
Cytoplasm 14.39 14.50 -4.21 -17.94 -16.06 8.79 13.15 -5.39 -9.26 -14.14 15.58 19.08 -4.05 -16.71 -20.15
Cytoplasmic Foci -13.26 13.65 40.53 43.80 5.46 -12.61 12.87 31.51 39.87 7.13 -17.26 18.87 49.00 57.31 7.22
Eisosomes 6.07 15.22 15.67 10.66 6.49 6.26 17.38 17.72 16.74 9.69 8.66 23.11 23.58 19.43 8.65
Endoplasmic Reticulum -3.19 -5.34 2.28 4.50 5.85 -3.04 -4.11 2.87 3.59 5.23 -3.78 -6.42 3.16 4.43 7.26
Endosome -9.53 0.60 9.99 15.58 6.20 -10.79 -1.96 8.28 15.91 8.34 -13.73 -0.43 10.41 20.54 8.26
Golgi -6.75 16.48 16.32 17.29 -0.49 -5.83 14.82 14.88 17.75 1.66 -8.65 22.12 21.66 24.38 -1.09
Lipid Particles -1.28 8.31 15.36 16.15 6.53 -2.24 9.40 18.42 17.91 7.76 -1.74 11.78 21.69 23.13 9.24
Mitochondria -2.96 7.62 7.20 9.28 -3.00 -5.64 7.41 4.31 8.47 -1.73 -5.97 10.49 9.44 13.28 -3.89
Mitotic Spindle -1.09 -0.65 -4.67 -3.62 -3.46 -2.82 -0.89 -1.98 -0.30 0.44 -2.86 -0.98 -4.97 -3.39 -0.79
None 3.76 3.69 4.04 0.20 0.13 3.21 1.85 -1.50 -2.82 -2.40 4.96 4.14 3.09 -1.13 -0.71
Nuclear Periphery -2.43 -9.36 -20.60 -4.22 6.26 -0.20 -11.36 -5.01 -4.91 4.02 -2.32 -14.55 -20.50 -10.54 9.00
Nuclear Periphery Foci -2.18 -4.86 0.51 2.30 4.87 -2.01 -7.66 2.60 4.16 8.29 -2.83 -6.01 -0.47 2.58 5.99
Nucleolus -1.42 -2.86 7.60 2.87 5.16 -2.92 -3.66 -0.95 1.88 1.98 -2.84 -4.42 5.51 4.36 6.69
Nucleus 10.90 -12.46 -30.70 -38.81 5.83 21.64 -9.00 -3.78 -12.60 6.22 23.13 -15.06 -25.13 -42.40 7.56
Peroxisomes -7.86 14.49 14.81 16.48 1.58 -8.67 12.30 13.89 18.67 3.14 -11.51 18.91 19.07 24.15 1.23
Vacuole -6.44 -7.43 -10.84 -4.68 5.49 -11.28 -9.23 -6.64 -3.62 6.41 -12.10 -11.78 -14.72 -7.20 8.69
Vacuole Periphery -7.04 6.58 4.57 9.84 -0.73 -6.24 2.35 2.93 9.67 1.29 -9.23 5.77 3.52 11.67 -0.83
Standard name
Human Ortholog
Description Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (23%), ER (3%), mixed (57%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bit61

Bit61


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bit61-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available