Standard name
Human Ortholog
Description Acetylglutamate synthase (glutamate N-acetyltransferase); mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 – 0 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0.06 0 0.07 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.08 0.05 0.06 – 0.05 0.05 0 0 0.09 0.08 0.11 0.05 0.12 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0.13 0 0 0.05 0.08 0 0 0 0 0 0 0
Endosome 0.09 0.1 0.11 0.07 0.23 0.07 0.07 – 0 0 0 0 0.13 0.19 0.22 0.2 0.06 0.17 0.08 0.11 0.07 0.13 0.11 0.06
Golgi 0 0 0.15 0.1 0.08 0 0 – 0 0 0 0 0.13 0.11 0.06 0.22 0.32 0.14 0 0.07 0 0.07 0.06 0.07
Mitochondria 0.89 0.76 0.57 0.8 0.55 0.81 0.74 – 0.88 0.86 0.93 0.88 0.48 0.42 0.33 0.14 0.16 0.15 0.74 0.64 0.71 0.63 0.63 0.67
Nucleus 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0.05 0.08 0 0 0 0 – 0 0 0 0 0 0 0.22 0.15 0.08 0.17 0.07 0.06 0.09 0.05 0 0.05
SpindlePole 0 0 0.11 0 0.08 0.08 0.06 – 0.08 0.11 0 0.15 0 0 0.06 0.21 0.13 0.22 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.19 0.2 0.05 0.13 0.06 0.06 – 0 0 0 0 0.17 0.16 0.19 0.13 0.16 0.2 0 0.06 0 0 0.09 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 3 3 1 0 3 – 4 1 0 0 1 6 0 3 1 4 0 7 4 0 0 3
Bud 5 5 1 11 3 9 8 – 8 7 15 23 0 3 0 3 3 7 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 2 – 0 3 0 2 0 0 0 0 0 2 0 0 0 0 0 0
Bud Site 3 0 0 0 0 1 2 – 1 5 4 6 0 0 0 3 1 1 – – – – – –
Cell Periphery 6 8 3 8 1 0 5 – 1 2 1 3 1 10 3 7 11 12 0 0 0 0 0 0
Cytoplasm 4 6 3 10 20 16 15 – 10 13 11 11 2 9 6 9 19 14 1 0 0 0 0 0
Endoplasmic Reticulum 1 3 4 4 4 3 2 – 1 0 1 5 3 5 1 9 13 6 0 1 2 0 0 1
Endosome 28 24 14 14 54 24 18 – 2 3 1 5 3 22 12 37 10 30 27 32 13 5 11 9
Golgi 2 8 20 21 20 0 4 – 0 1 0 0 3 13 3 41 51 24 14 20 4 3 6 11
Mitochondria 291 178 75 164 132 269 190 – 192 236 235 302 11 49 18 25 25 26 249 195 129 28 65 114
Nucleus 7 5 3 3 3 8 12 – 8 7 4 3 1 2 0 4 2 5 0 1 0 0 1 0
Nuclear Periphery 6 4 0 0 3 3 8 – 4 7 5 10 0 0 0 3 1 0 4 3 0 0 0 2
Nucleolus 6 1 1 0 5 13 6 – 3 1 2 7 0 0 0 4 1 4 0 0 0 0 0 0
Peroxisomes 17 12 11 4 5 4 3 – 3 6 4 7 0 5 12 27 12 30 23 18 16 2 4 8
SpindlePole 13 7 14 6 20 25 16 – 18 30 10 50 0 4 3 39 21 39 5 2 1 1 1 1
Vac/Vac Membrane 35 44 26 11 31 20 16 – 5 9 6 8 4 19 10 24 26 34 4 18 6 1 8 14
Unique Cell Count 328 234 131 205 238 331 257 219 274 252 344 23 116 54 185 159 174 339 306 182 46 104 170
Labelled Cell Count 424 307 178 259 302 395 310 260 331 299 442 29 147 68 238 197 238 339 306 182 46 104 170


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 6.2 6.8 6.4 6.8 5.4 5.7 – 4.5 4.4 4.1 4.3 7.0 6.7 7.0 7.7 7.9 7.5 – 5.9 6.8
Std Deviation (1e-4) 0.8 1.4 1.1 1.1 1.5 1.3 1.7 – 1.4 1.2 1.4 1.5 0.9 1.0 1.8 1.2 1.5 1.6 – 1.1 1.1
Intensity Change (Log2) – – – -0.08 0.01 -0.34 -0.26 – -0.6 -0.61 -0.72 -0.66 0.04 -0.03 0.04 0.17 0.22 0.15 – -0.19 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 2.2 0 0 1.8 1.8
Cytoplasm 1.2 2.3 1.2 1.6 – 0 1.2 0 0 0 2.0 0 0 3.1 2.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0.8 1.8 0
Endosome -1.2 2.8 -1.2 -1.2 – 0 0 0 0 0 1.8 2.1 2.2 -1.4 1.6
Golgi -1.4 -2.0 -7.3 -5.3 – 0 0 0 0 0 -0.9 -1.8 1.5 3.3 -0.4
Mitochondria 4.5 -0.3 5.3 3.3 – 0 0 0 0 -0.8 -2.4 -3.0 -8.2 -7.4 -7.8
Nucleus 0 0 0 1.1 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes -2.8 -2.8 0 0 – 0 0 0 0 0 -1.3 2.6 1.7 -0.3 2.2
SpindlePole -2.9 -0.7 -1.1 -1.6 – 0 0 0 0 0 -2.2 0 2.4 0.7 2.7
Vacuole -4.1 -1.7 -4.5 -4.1 – -5.6 -5.5 -5.9 -6.6 0 -0.7 -0.2 -1.6 -0.8 -0.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8386 2.4442 1.9855 2.0647 1.9813 2.3321 2.0122 2.1166 1.9104 2.0589 1.7065 2.1118 2.5959 2.4454 2.1218 2.2801 2.1548 2.2567
Actin 0.0237 0.004 0.0111 0.0002 0.0029 0.0028 0.0111 0.0119 0.004 0.0038 0.0229 0.0032 0.059 0.0168 0.0215 0.0429 0.0008 0.0075
Bud 0.0003 0.0008 0.0002 0 0.0015 0.0001 0.0002 0.0009 0.0003 0.0002 0.0025 0.0002 0.0024 0.0061 0.0024 0.0006 0.0001 0.0005
Bud Neck 0.0004 0.0005 0.0007 0.0001 0.0003 0.0002 0.0005 0.0003 0.0002 0.0003 0.0003 0.0002 0.0026 0.0003 0.0004 0.0116 0 0.0006
Bud Periphery 0.0005 0.0017 0.0004 0.0001 0.0085 0.0005 0.0005 0.0018 0.0005 0.0005 0.0114 0.0005 0.0052 0.0045 0.0077 0.0006 0.0001 0.0006
Bud Site 0.0015 0.0054 0.0008 0.0001 0.0007 0.0002 0.0006 0.0044 0.0008 0.0003 0.0009 0.0002 0.0055 0.0075 0.0025 0.0028 0.0001 0.0012
Cell Periphery 0.0002 0.0004 0.0002 0.0001 0.0002 0.0002 0.0002 0.0004 0.0001 0.0002 0.0001 0.0001 0.0003 0.0004 0.0002 0.0002 0 0.0004
Cytoplasm 0.0004 0.0004 0.0005 0.0001 0.0002 0.0001 0.002 0.0016 0.0003 0.0002 0.0019 0.0002 0.0082 0.0017 0.0003 0.0004 0 0.0072
Cytoplasmic Foci 0.0082 0.0189 0.0154 0.0026 0.0148 0.0058 0.0177 0.0206 0.0087 0.0086 0.0036 0.006 0.0312 0.0168 0.0122 0.0469 0.0009 0.013
Eisosomes 0.0004 0.0002 0.0003 0.0001 0.0001 0.0001 0.0003 0.0004 0.0002 0.0003 0.0003 0.0002 0.0006 0.0003 0.0003 0.0004 0.0001 0.0004
Endoplasmic Reticulum 0.0005 0.0003 0.0002 0 0.0001 0.0001 0.0014 0.0017 0.0002 0.0001 0.0002 0.0002 0.0035 0.0004 0.0002 0.0002 0 0.0002
Endosome 0.0278 0.0427 0.0254 0.0209 0.0053 0.0202 0.0423 0.044 0.0156 0.0089 0.0073 0.0111 0.0352 0.0279 0.0242 0.014 0.0034 0.0299
Golgi 0.0307 0.0531 0.0252 0.0223 0.0095 0.0415 0.0407 0.0534 0.0274 0.0266 0.0095 0.0235 0.0437 0.0646 0.0562 0.0211 0.0101 0.0861
Lipid Particles 0.0274 0.0292 0.0256 0.0031 0.0181 0.0218 0.0343 0.028 0.0055 0.0015 0.0065 0.0096 0.0523 0.0218 0.0171 0.0713 0.0011 0.009
Mitochondria 0.8407 0.7776 0.8388 0.9115 0.8809 0.8684 0.7853 0.7713 0.9056 0.9318 0.8271 0.9138 0.6718 0.762 0.8048 0.7079 0.9712 0.8035
None 0.0011 0.0002 0.0004 0 0.0004 0.0001 0.0038 0.0044 0.0002 0.0001 0.03 0.0002 0.0048 0.0008 0.0002 0.0016 0 0.0001
Nuclear Periphery 0.0012 0.0008 0.001 0 0.001 0.0001 0.0076 0.0031 0.0001 0 0.002 0.0019 0.008 0.0002 0.0001 0.0001 0 0.0001
Nucleolus 0.0004 0.0004 0.0002 0.0001 0.0003 0.0003 0.0006 0.0006 0.0001 0 0.002 0.0002 0.0006 0.0003 0.0002 0.0018 0 0.0003
Nucleus 0.0005 0.0002 0.0001 0 0.0002 0.0001 0.0025 0.0009 0.0001 0 0.0076 0.0005 0.0045 0.0003 0.0001 0.0002 0 0.0001
Peroxisomes 0.0297 0.0533 0.0475 0.036 0.0507 0.0332 0.0386 0.0431 0.028 0.0152 0.0548 0.0259 0.0485 0.0643 0.045 0.0697 0.0115 0.0351
Punctate Nuclear 0.0008 0.0002 0.0001 0 0.0001 0 0.0035 0.0003 0.0001 0 0.007 0.0006 0.0055 0.0001 0.0001 0.0023 0 0.0001
Vacuole 0.0017 0.0049 0.0022 0.0011 0.0009 0.0015 0.0029 0.003 0.0008 0.0004 0.0009 0.0007 0.0039 0.0015 0.0024 0.0015 0.0001 0.0017
Vacuole Periphery 0.0019 0.0048 0.0035 0.0015 0.0033 0.0028 0.0035 0.004 0.0012 0.0008 0.0012 0.0012 0.0029 0.0015 0.0021 0.0019 0.0005 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.2345 9.7208 6.3646 9.7694 12.4401 10.8681 10.2623 11.3163 9.1661 11.1544
Translational Efficiency 0.4404 0.46 0.5892 0.521 0.3223 0.4646 0.3456 0.3742 0.583 0.4321

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Acetylglutamate synthase (glutamate N-acetyltransferase); mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Arg2

Arg2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available