Standard name
Human Ortholog
Description Subunit of the multiprotein cohesin complex; required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0.06 0.05 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0 0.07 0.1 0.06 0.11 0.11 0.08 0.07 – 0.08 0 0.13 0.2 0.24 0.19 0.22 0.29 0 0 0 0.06 0.11 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.1 0 0 0 0.11 0 0 0 0 – 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.14 0.4 0.16 0.3 0.26 0.37 0.51 – 0.43 0.52 0.09 0 0.11 0.06 0 0.05 0 0 0 0 0 0
Nucleus 0.54 0.56 0.55 0.43 0.36 0.42 0.4 0.45 0.45 – 0.52 0.49 0.49 0.39 0.38 0.46 0.43 0.42 0.5 0.58 0.48 0.68 0.5 0.35
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.23 0.25 0.28 0.37 0.28 0.3 0.32 0.29 – 0.26 0.28 0.27 0.31 0.27 0.18 0.2 0.13 0.11 0.15 0.12 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0 0.17 0.11 0.14 0 0 0
Vac/Vac Membrane 0.22 0.27 0.09 0.08 0.14 0.13 0.12 0.05 0 – 0 0.06 0.13 0.13 0.1 0.21 0.19 0.16 0.07 0.06 0.11 0.14 0.2 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 1
Bud 1 1 1 0 2 3 1 2 6 – 6 6 0 0 1 1 0 0 18 15 17 8 15 8
Bud Neck 0 0 1 0 0 0 0 4 0 – 2 3 0 0 0 0 0 0 1 0 0 0 1 3
Bud Site 0 1 2 2 7 11 8 14 14 – 12 21 0 0 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 26 13 18 19 17 37 40 33 21 – 21 11 28 42 56 36 33 56 12 5 6 10 30 54
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 6 5 11 1 0 1 0 3 4
Endosome 33 10 10 8 31 4 6 4 1 – 0 1 10 8 7 8 6 10 12 6 8 1 5 8
Golgi 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 2 1 1 1 0 0 1 3
Mitochondria 2 13 38 73 44 98 92 145 161 – 118 187 20 9 26 11 3 9 2 3 2 2 4 6
Nucleus 188 168 147 78 101 138 142 177 142 – 143 176 103 82 87 88 63 82 197 195 148 125 137 98
Nuclear Periphery 1 2 0 0 0 2 5 5 4 – 3 2 0 1 1 0 0 0 4 1 2 0 0 1
Nucleolus 70 68 67 51 102 91 104 128 92 – 72 102 57 64 61 35 29 26 43 51 37 4 3 5
Peroxisomes 0 0 0 0 2 1 0 0 0 – 0 0 0 1 2 0 0 1 0 0 1 0 0 0
SpindlePole 19 5 6 4 20 6 6 3 2 – 1 4 2 7 4 0 1 1 68 37 42 4 8 9
Vac/Vac Membrane 77 81 25 15 39 43 41 20 12 – 10 22 28 27 24 41 28 31 28 21 33 25 55 64
Unique Cell Count 345 301 268 182 278 329 351 395 317 276 362 212 209 230 192 148 193 393 340 306 184 275 281
Labelled Cell Count 418 362 315 251 365 434 445 535 455 388 535 248 242 270 229 170 228 393 340 306 184 275 281


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 6.3 5.2 5.0 5.9 5.5 5.4 5.2 4.7 – 4.8 4.5 6.5 6.7 5.7 9.2 11.6 10.9 6.4 6.1 6.3
Std Deviation (1e-4) 1.2 1.1 0.9 1.2 1.5 1.6 1.7 1.7 1.2 – 1.2 1.2 1.9 1.9 1.7 2.1 3.4 3.1 1.9 1.9 1.5
Intensity Change (Log2) – – – -0.06 0.17 0.09 0.05 0.0 -0.14 – -0.13 -0.21 0.31 0.35 0.13 0.82 1.16 1.06 0.3 0.24 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 2.2 0 2.3 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 1.4 -0.3 1.9 2.0 0.8 0 – 0.4 -2.2 2.4 4.4 5.5 4.0 4.6 6.4
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0.4 3.3 -2.0 -1.6 -2.4 0 – 0 0 0.5 0.1 -0.4 0.2 0.2 0.8
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 6.3 0.5 4.5 3.6 6.4 0 – 0 0 -1.6 -3.6 -1.0 -2.9 -4.0 -3.3
Nucleus -2.5 -4.3 -3.1 -3.6 -2.5 -2.4 – -0.7 -1.5 -1.4 -3.4 -3.8 -1.9 -2.4 -2.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0.7 3.0 0.7 1.3 2.1 0 – 0 0 0.5 1.4 0.4 -1.7 -1.3 -3.0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 2.7 -0.4 -0.5 0 0 – 0 0 0 0.7 0 0 0 0
Vacuole -0.4 1.7 1.4 0.9 -2.1 -2.7 – -2.7 -1.5 1.3 1.2 0.4 3.6 2.8 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.4034 8.3768 7.9548 7.2593 7.1505 7.1731 4.3571 6.3016 6.0626 5.8675 5.0242 5.2948 4.2135 4.9986 5.1209 5.9672 4.7069 4.7432
Actin 0.0001 0 0 0.0004 0.0001 0.0001 0.0266 0.0007 0.0056 0.0094 0.0016 0.0039 0.0011 0.0069 0.0311 0.0009 0 0
Bud 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.001 0.0002 0.0007 0.0004 0.0001 0.0003 0.0001 0.0002 0.0006 0.0001 0 0
Bud Neck 0.0005 0.0006 0.0009 0.0015 0.0023 0.0031 0.006 0.0006 0.0007 0.0009 0.001 0.0018 0.0006 0.0049 0.002 0.0025 0.0003 0.0018
Bud Periphery 0 0.0002 0.0001 0.0001 0.0002 0.0001 0.0017 0.0003 0.0008 0.0004 0.0001 0.0006 0.0001 0.0005 0.0011 0.0005 0 0.0001
Bud Site 0.0003 0.0004 0.0004 0.0007 0.0015 0.0001 0.019 0.0011 0.0026 0.0019 0.001 0.0003 0.0008 0.0115 0.0029 0.0003 0 0.0001
Cell Periphery 0 0.0001 0 0.0001 0.0001 0 0.0006 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0021 0.0002 0.0001 0 0
Cytoplasm 0.0013 0.002 0.0002 0.001 0.0006 0.0003 0.0235 0.0008 0.0019 0.0006 0.0266 0.0006 0.01 0.0019 0.0007 0.0095 0.0002 0.0009
Cytoplasmic Foci 0.0001 0.0001 0.0001 0.0002 0.0004 0.0001 0.0313 0.0009 0.008 0.0127 0.0343 0.0058 0.0022 0.0005 0.0058 0.0005 0 0.0001
Eisosomes 0 0 0 0 0 0 0.0004 0 0.0001 0 0 0.0001 0 0.0021 0.0001 0 0 0
Endoplasmic Reticulum 0.0001 0.0003 0 0.0001 0.0001 0 0.0085 0.0003 0.0026 0.0002 0.0014 0.0003 0.0013 0.0003 0.0016 0.0002 0 0
Endosome 0.0003 0.0006 0.0001 0.0002 0.0004 0.0001 0.0389 0.0016 0.0118 0.013 0.0088 0.0061 0.0142 0.0008 0.0073 0.0006 0 0
Golgi 0 0 0 0 0.0002 0 0.0149 0.0005 0.0042 0.0321 0.0049 0.0084 0.0007 0.0015 0.0077 0.0001 0 0
Lipid Particles 0.0001 0.0001 0 0 0.0003 0.0001 0.054 0.003 0.0098 0.0052 0.0682 0.014 0.0018 0.0008 0.0053 0.0001 0 0
Mitochondria 0.0001 0.0002 0.0001 0.0001 0.0003 0.0001 0.0185 0.0022 0.0112 0.0032 0.0006 0.0197 0.0008 0.0039 0.0138 0.0005 0.0001 0.0001
None 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0229 0.0004 0.0009 0.0004 0.0262 0.0002 0.0108 0.0002 0.0003 0.002 0 0.0109
Nuclear Periphery 0.0157 0.004 0.0015 0.0052 0.0019 0.0016 0.0194 0.0039 0.0124 0.0019 0.0064 0.0023 0.0249 0.0043 0.0086 0.0088 0.0008 0.0009
Nucleolus 0.0406 0.0572 0.083 0.0478 0.0675 0.0602 0.0713 0.0659 0.0915 0.0467 0.0216 0.0358 0.0738 0.063 0.1049 0.0643 0.0158 0.0775
Nucleus 0.8716 0.8879 0.7959 0.8063 0.9023 0.8865 0.578 0.8912 0.7207 0.772 0.7206 0.8856 0.7803 0.8192 0.6568 0.6807 0.9324 0.8599
Peroxisomes 0 0 0 0 0.0001 0 0.0247 0.0004 0.0102 0.0152 0.0418 0.0039 0.0006 0.0005 0.0051 0.0001 0 0
Punctate Nuclear 0.0686 0.045 0.1175 0.1354 0.0213 0.047 0.0327 0.0249 0.1011 0.0834 0.0319 0.0092 0.0738 0.074 0.143 0.2259 0.0504 0.0476
Vacuole 0.0003 0.0009 0.0001 0.0004 0.0003 0.0003 0.0036 0.0007 0.0021 0.0003 0.0015 0.0005 0.0013 0.0007 0.0008 0.0015 0 0.0001
Vacuole Periphery 0.0001 0.0002 0 0 0.0001 0 0.0024 0.0004 0.001 0.0002 0.0012 0.0007 0.0008 0.0002 0.0005 0.0009 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 62.8938 19.7649 8.5224 19.7699 46.8736 53.1539 11.9501 9.387 13.4153 47.4002
Translational Efficiency 1.438 1.0399 1.1153 1.0586 1.1073 1.3096 1.0437 0.7808 0.8768 0.9582

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the multiprotein cohesin complex; required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
Localization
Cell Percentages nucleus (60%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Smc3

Smc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available