Standard name | |
---|---|
Human Ortholog | |
Description | Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 2 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 18 | 50 | 30 | 37 | 37 | 45 | 58 | 78 | 56 | 71 | 64 | 72 | 5 | 93 | 190 | 20 | 10 | 11 | 6 | 7 | 2 | 2 | 3 | 9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 15 | 31 | 9 | 5 | 8 | 5 | 6 | 5 | 6 | 4 | 0 | 2 | 4 | 4 | 1 | 4 | 2 | 2 | 12 | 37 | 8 | 1 | 6 | 11 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 5 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 0 | 0 | 1 | 0 |
Mitochondria | 19 | 60 | 2 | 0 | 3 | 12 | 5 | 3 | 19 | 13 | 10 | 11 | 5 | 0 | 0 | 2 | 7 | 0 | 2 | 9 | 3 | 1 | 1 | 6 |
Nucleus | 37 | 100 | 45 | 52 | 53 | 85 | 151 | 147 | 118 | 104 | 78 | 87 | 21 | 30 | 32 | 64 | 46 | 35 | 6 | 15 | 5 | 3 | 3 | 1 |
Nuclear Periphery | 1 | 10 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 3 | 3 | 4 | 2 | 1 | 3 | 2 | 3 |
Nucleolus | 2 | 8 | 0 | 1 | 2 | 1 | 1 | 3 | 6 | 0 | 3 | 2 | 1 | 0 | 0 | 1 | 4 | 1 | 2 | 3 | 0 | 0 | 0 | 2 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 2 | 6 | 3 | 1 | 3 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 5 | 2 | 0 | 0 | 1 |
Vac/Vac Membrane | 123 | 333 | 95 | 77 | 146 | 169 | 274 | 293 | 230 | 268 | 209 | 250 | 53 | 62 | 63 | 69 | 55 | 40 | 164 | 434 | 142 | 47 | 80 | 100 |
Unique Cell Count | 181 | 489 | 159 | 141 | 206 | 251 | 415 | 421 | 352 | 402 | 307 | 345 | 72 | 165 | 241 | 142 | 106 | 78 | 202 | 531 | 177 | 65 | 108 | 140 |
Labelled Cell Count | 217 | 600 | 186 | 173 | 254 | 317 | 498 | 532 | 441 | 471 | 369 | 430 | 92 | 190 | 288 | 163 | 129 | 95 | 202 | 531 | 177 | 65 | 108 | 140 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 6.2 | 7.8 | 8.4 | 10.3 | 8.9 | 10.4 | 11.6 | 11.9 | 13.9 | 12.8 | 13.2 | 7.0 | 5.9 | 5.2 | 8.5 | 9.6 | 11.3 | 7.6 | 7.1 | 6.5 |
Std Deviation (1e-4) | 1.7 | 1.8 | 2.6 | 2.9 | 3.8 | 3.5 | 3.8 | 4.5 | 5.3 | 5.8 | 5.3 | 5.7 | 2.2 | 1.6 | 1.3 | 2.9 | 3.2 | 4.2 | 2.7 | 2.5 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.4 | 0.18 | 0.42 | 0.57 | 0.61 | 0.83 | 0.71 | 0.76 | -0.16 | -0.41 | -0.58 | 0.13 | 0.3 | 0.53 | -0.05 | -0.14 | -0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | -0.2 | -0.2 | -1.5 | -0.1 | -0.8 | -0.3 | 0.5 | 0.5 | -2.3 | 7.0 | 11.8 | -1.1 | -2.1 | -0.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.9 | -0.8 | -2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.5 | 0 | -1.2 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1.9 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.6 | -0.6 | 1.2 | 1.8 | 1.5 | 1.2 | -0.6 | -0.7 | -0.7 | 0.1 | -2.2 | -3.7 | 3.0 | 2.5 | 2.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.9 | 2.2 | 1.6 | 1.4 | 2.2 | 1.2 | 1.5 | 1.8 | 2.9 | 2.0 | -4.0 | -6.7 | -1.9 | -1.3 | -1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8454 | 5.3377 | 3.6036 | 3.3615 | 3.2923 | 4.3261 | 6.1254 | 6.3324 | 6.2558 | 4.9111 | 5.2801 | 6.9045 | 2.0232 | 3.6957 | 2.5469 | 1.9695 | 3.0405 | 2.9383 |
Actin | 0.0351 | 0 | 0.0217 | 0 | 0.0035 | 0 | 0.0144 | 0 | 0.0071 | 0.0004 | 0.0025 | 0.0038 | 0.0398 | 0.0043 | 0.0125 | 0.0071 | 0.0025 | 0.0061 |
Bud | 0.0005 | 0.0008 | 0.0005 | 0.0002 | 0.002 | 0.0002 | 0.0008 | 0.0013 | 0.0012 | 0.0007 | 0.0003 | 0.0008 | 0.0009 | 0.0018 | 0.0016 | 0.0008 | 0.0003 | 0.0004 |
Bud Neck | 0.0029 | 0.0009 | 0.001 | 0.0008 | 0.0029 | 0.0019 | 0.0011 | 0.0008 | 0.0016 | 0.0015 | 0.0007 | 0.0019 | 0.0051 | 0.0021 | 0.0013 | 0.001 | 0.0018 | 0.0018 |
Bud Periphery | 0.0012 | 0.0013 | 0.0016 | 0.0006 | 0.0036 | 0.0009 | 0.0018 | 0.0037 | 0.0043 | 0.0016 | 0.0005 | 0.0019 | 0.0017 | 0.0027 | 0.0026 | 0.0026 | 0.0008 | 0.0014 |
Bud Site | 0.0171 | 0.0017 | 0.0023 | 0.0006 | 0.0137 | 0.0007 | 0.0022 | 0.0016 | 0.0018 | 0.0019 | 0.001 | 0.0013 | 0.0063 | 0.0043 | 0.0028 | 0.0014 | 0.0013 | 0.0009 |
Cell Periphery | 0.0059 | 0.0045 | 0.0056 | 0.0034 | 0.0044 | 0.0059 | 0.0051 | 0.0071 | 0.0048 | 0.0038 | 0.0027 | 0.0063 | 0.0024 | 0.0048 | 0.0052 | 0.0015 | 0.0027 | 0.0041 |
Cytoplasm | 0.0023 | 0.0065 | 0.0067 | 0.0035 | 0.0044 | 0.0013 | 0.0058 | 0.0033 | 0.002 | 0.002 | 0.0011 | 0.0038 | 0.0051 | 0.0165 | 0.0136 | 0.0039 | 0.0038 | 0.0023 |
Cytoplasmic Foci | 0.0166 | 0.0065 | 0.0165 | 0.0056 | 0.0114 | 0.0054 | 0.0282 | 0.0066 | 0.0128 | 0.0197 | 0.0408 | 0.0231 | 0.027 | 0.015 | 0.0244 | 0.0067 | 0.0218 | 0.0167 |
Eisosomes | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.002 | 0.0035 | 0.0018 | 0.001 | 0.0015 | 0.0011 | 0.0021 | 0.0019 | 0.0016 | 0.0013 | 0.0007 | 0.002 | 0.0052 | 0.0023 | 0.0038 | 0.0009 | 0.001 | 0.0022 |
Endosome | 0.1529 | 0.0836 | 0.1197 | 0.1484 | 0.1237 | 0.1452 | 0.1579 | 0.1222 | 0.1733 | 0.1455 | 0.1812 | 0.2007 | 0.2602 | 0.144 | 0.2028 | 0.1941 | 0.1445 | 0.2276 |
Golgi | 0.0141 | 0.0027 | 0.0096 | 0.0027 | 0.005 | 0.0056 | 0.0184 | 0.0047 | 0.0136 | 0.0084 | 0.0216 | 0.0138 | 0.0293 | 0.0059 | 0.0144 | 0.0049 | 0.034 | 0.0098 |
Lipid Particles | 0.0846 | 0.0194 | 0.0801 | 0.0195 | 0.0384 | 0.0507 | 0.0991 | 0.0528 | 0.065 | 0.0288 | 0.0626 | 0.0756 | 0.0706 | 0.046 | 0.0448 | 0.023 | 0.0403 | 0.0502 |
Mitochondria | 0.0486 | 0.0139 | 0.0897 | 0.0272 | 0.0086 | 0.0147 | 0.0643 | 0.0405 | 0.0999 | 0.0308 | 0.0315 | 0.0117 | 0.0623 | 0.0124 | 0.0774 | 0.0211 | 0.0209 | 0.0156 |
None | 0.0174 | 0.0004 | 0.0015 | 0.0043 | 0.0552 | 0.0002 | 0.028 | 0.0006 | 0.0097 | 0.0322 | 0.0006 | 0.0004 | 0.0129 | 0.0036 | 0.0176 | 0.0255 | 0.0039 | 0.0008 |
Nuclear Periphery | 0.0051 | 0.0076 | 0.0182 | 0.0023 | 0.0031 | 0.0036 | 0.0043 | 0.0046 | 0.0108 | 0.0037 | 0.001 | 0.0057 | 0.0141 | 0.01 | 0.008 | 0.002 | 0.0009 | 0.006 |
Nucleolus | 0.0293 | 0.0044 | 0.0142 | 0.0103 | 0.0368 | 0.0075 | 0.0167 | 0.0055 | 0.012 | 0.0115 | 0.0043 | 0.0129 | 0.0073 | 0.0178 | 0.0128 | 0.0047 | 0.0092 | 0.0147 |
Nucleus | 0.0076 | 0.0036 | 0.0042 | 0.0025 | 0.0466 | 0.0034 | 0.0064 | 0.005 | 0.0129 | 0.0037 | 0.002 | 0.0058 | 0.0064 | 0.0153 | 0.0104 | 0.0026 | 0.0026 | 0.0082 |
Peroxisomes | 0.027 | 0.0006 | 0.011 | 0.0015 | 0.007 | 0.0012 | 0.0221 | 0.0026 | 0.0059 | 0.003 | 0.0042 | 0.0089 | 0.0196 | 0.0041 | 0.0143 | 0.0019 | 0.0439 | 0.0072 |
Punctate Nuclear | 0.0061 | 0.0002 | 0.0005 | 0.0001 | 0.0019 | 0.0001 | 0.0017 | 0.0001 | 0.0004 | 0.0017 | 0.0006 | 0.0004 | 0.0052 | 0.0014 | 0.0009 | 0.0002 | 0.0004 | 0.0021 |
Vacuole | 0.3409 | 0.5726 | 0.3962 | 0.5936 | 0.4589 | 0.5655 | 0.3421 | 0.5042 | 0.3815 | 0.5392 | 0.4973 | 0.4278 | 0.2771 | 0.5097 | 0.368 | 0.5233 | 0.5271 | 0.4638 |
Vacuole Periphery | 0.1825 | 0.265 | 0.1973 | 0.172 | 0.1675 | 0.1848 | 0.1773 | 0.2307 | 0.1776 | 0.1589 | 0.143 | 0.1912 | 0.1407 | 0.176 | 0.1608 | 0.1708 | 0.1361 | 0.1579 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 44.7492 | 16.4396 | 121.9889 | 72.8614 | 19.0748 | 139.6872 | 47.927 | 75.9137 | 76.1294 | 34.285 |
Translational Efficiency | 1.4572 | 1.4514 | 1.6124 | 1.5848 | 1.642 | 1.342 | 1.1858 | 1.9884 | 1.9544 | 1.5352 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1788 | 1856 | 2020 | 1943 | 2417 | 1132 | 1621 | 1543 | 4205 | 2988 | 3641 | 3486 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1100.26 | 1055.34 | 1126.61 | 1044.16 | 1032.63 | 1036.71 | 1199.82 | 1081.11 | 1061.39 | 1048.28 | 1159.20 | 1060.52 |
Standard Deviation | 245.94 | 233.41 | 250.08 | 277.67 | 227.05 | 201.47 | 340.33 | 263.21 | 237.63 | 222.04 | 295.95 | 271.99 |
Intensity Change Log 2 | — | -0.060137 | 0.034144 | -0.075502 | — | 0.005689 | 0.216495 | 0.066190 | — | -0.027892 | 0.125308 | -0.005163 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000018 | 0.000030 | 0.000196 | 0.001961 | 0.000011 | 0.000018 | 0.000259 | 0.001703 | 0.000014 | 0.000025 | 0.000224 | 0.001847 |
Bud Neck | 0.003611 | 0.006038 | 0.015952 | 0.020029 | 0.002558 | 0.007808 | 0.014698 | 0.021510 | 0.003006 | 0.006709 | 0.015394 | 0.020684 |
Bud Site | 0.006878 | 0.010269 | 0.021479 | 0.076468 | 0.003213 | 0.007893 | 0.021225 | 0.079163 | 0.004772 | 0.009369 | 0.021366 | 0.077661 |
Cell Periphery | 0.006915 | 0.004287 | 0.001089 | 0.003294 | 0.006427 | 0.003613 | 0.001330 | 0.003196 | 0.006634 | 0.004031 | 0.001196 | 0.003251 |
Cytoplasm | 0.001231 | 0.003437 | 0.006168 | 0.086658 | 0.001060 | 0.003732 | 0.005437 | 0.089784 | 0.001133 | 0.003549 | 0.005842 | 0.088042 |
Cytoplasmic Foci | 0.001006 | 0.001560 | 0.028007 | 0.059222 | 0.000425 | 0.002431 | 0.029107 | 0.050478 | 0.000672 | 0.001890 | 0.028496 | 0.055352 |
Eisosomes | 0.000180 | 0.000072 | 0.000089 | 0.000039 | 0.000159 | 0.000070 | 0.000079 | 0.000041 | 0.000168 | 0.000071 | 0.000085 | 0.000040 |
Endoplasmic Reticulum | 0.002209 | 0.002710 | 0.001772 | 0.014236 | 0.003116 | 0.003587 | 0.005885 | 0.017291 | 0.002730 | 0.003042 | 0.003603 | 0.015588 |
Endosome | 0.026192 | 0.031702 | 0.187105 | 0.199134 | 0.012317 | 0.052944 | 0.177738 | 0.201622* | 0.018216 | 0.039749 | 0.182935 | 0.200235* |
Golgi | 0.002571 | 0.002301 | 0.016520 | 0.027450 | 0.000741 | 0.001750 | 0.019922 | 0.021134 | 0.001519 | 0.002092 | 0.018035 | 0.024655 |
Lipid Particles | 0.000877 | 0.000395 | 0.010502 | 0.012854 | 0.000551 | 0.000858 | 0.023008 | 0.009980 | 0.000690 | 0.000570 | 0.016070 | 0.011582 |
Mitochondria | 0.004773 | 0.001663 | 0.008015 | 0.004436 | 0.001833 | 0.005398 | 0.009945 | 0.003272 | 0.003083 | 0.003078 | 0.008874 | 0.003921 |
Mitotic Spindle | 0.000060 | 0.000244 | 0.005010 | 0.021882 | 0.000028 | 0.000315 | 0.006317 | 0.018895 | 0.000041 | 0.000271 | 0.005592 | 0.020560 |
None | 0.000298 | 0.000291 | 0.000344 | 0.000313 | 0.000132 | 0.000223 | 0.000317 | 0.000240 | 0.000202 | 0.000265 | 0.000332 | 0.000281 |
Nuclear Periphery | 0.000205 | 0.000356 | 0.001733 | 0.000592 | 0.000336 | 0.000747 | 0.004813 | 0.000533 | 0.000280 | 0.000504 | 0.003104 | 0.000566 |
Nuclear Periphery Foci | 0.000334 | 0.000393 | 0.007447 | 0.002456 | 0.000425 | 0.001019 | 0.013313 | 0.003067 | 0.000386 | 0.000630 | 0.010059 | 0.002727 |
Nucleolus | 0.001909 | 0.002091 | 0.009862 | 0.001221 | 0.001677 | 0.002671 | 0.008432 | 0.001410 | 0.001776 | 0.002311 | 0.009225 | 0.001304 |
Nucleus | 0.001389 | 0.003405 | 0.007309 | 0.007405 | 0.002232 | 0.003036 | 0.006617 | 0.005741 | 0.001874 | 0.003265 | 0.007001 | 0.006669 |
Peroxisomes | 0.000306 | 0.000442 | 0.004147 | 0.006759 | 0.000173 | 0.000462 | 0.003849 | 0.006487 | 0.000230 | 0.000450 | 0.004014 | 0.006639 |
Vacuole | 0.772973* | 0.782635* | 0.526614* | 0.421830* | 0.851411* | 0.795796* | 0.512693* | 0.428023* | 0.818058* | 0.787621* | 0.520416* | 0.424571* |
Vacuole Periphery | 0.166067* | 0.145679* | 0.140641* | 0.031761 | 0.111175* | 0.105629* | 0.135017* | 0.036429 | 0.134516* | 0.130506* | 0.138137* | 0.033827 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.74 | -2.55 | -5.90 | -5.86 | -5.08 | -2.01 | -6.79 | -3.87 | -3.86 | -3.28 | -2.56 | -4.93 | -6.75 | -6.70 | -5.79 |
Bud Neck | -3.66 | -12.69 | -14.16 | -11.68 | -2.71 | -5.46 | -13.88 | -13.96 | -8.58 | -4.27 | -7.03 | -18.92 | -20.23 | -14.51 | -4.88 |
Bud Site | -2.77 | -10.31 | -19.80 | -18.66 | -14.65 | -3.85 | -11.32 | -20.08 | -18.07 | -13.81 | -5.54 | -16.09 | -28.48 | -25.92 | -20.13 |
Cell Periphery | 4.02 | 11.30 | 5.43 | 2.19 | -2.85 | 4.46 | 10.11 | 5.15 | 1.23 | -2.36 | 5.72 | 15.30 | 7.39 | 2.55 | -3.70 |
Cytoplasm | -2.90 | -6.19 | -24.90 | -24.19 | -23.12 | -2.53 | -4.58 | -21.69 | -20.87 | -20.22 | -3.88 | -7.66 | -33.03 | -31.96 | -30.71 |
Cytoplasmic Foci | -1.55 | -14.15 | -22.22 | -21.99 | -8.44 | -3.45 | -13.90 | -19.38 | -18.04 | -5.57 | -4.16 | -19.89 | -29.52 | -28.52 | -10.05 |
Eisosomes | 5.64 | 5.04 | 8.08 | 4.20 | 9.89 | 5.76 | 5.48 | 7.98 | 3.06 | 4.69 | 7.96 | 7.37 | 11.54 | 5.07 | 9.94 |
Endoplasmic Reticulum | -2.09 | 2.43 | -6.14 | -5.80 | -6.39 | -1.51 | -2.35 | -5.17 | -4.95 | -3.50 | -1.60 | -1.66 | -7.85 | -7.59 | -6.70 |
Endosome | -2.02 | -29.49* | -26.46* | -25.01 | 2.30 | -10.79 | -31.22* | -27.63 | -17.88 | 0.05 | -10.16 | -43.35* | -38.91* | -30.93 | 1.77 |
Golgi | 0.67 | -15.12 | -12.27 | -12.45 | -3.42 | -2.80 | -16.70 | -12.86 | -11.72 | 0.99 | -2.29 | -23.04 | -17.59 | -16.85 | -2.10 |
Lipid Particles | 4.93 | -7.90 | -7.85 | -8.23 | -0.51 | -1.69 | -9.61 | -6.18 | -5.91 | 5.10 | 1.40 | -12.36 | -10.04 | -10.15 | 3.58 |
Mitochondria | 3.51 | -2.82 | 0.75 | -2.67 | 3.71 | -2.58 | -6.27 | -2.12 | 1.67 | 5.40 | 0.00 | -7.04 | -0.74 | -0.69 | 6.37 |
Mitotic Spindle | -2.66 | -6.25 | -11.50 | -11.42 | -8.73 | -2.34 | -6.56 | -9.67 | -9.54 | -5.82 | -3.63 | -9.07 | -15.01 | -14.86 | -10.44 |
None | 0.09 | -0.62 | -0.81 | -1.07 | -0.30 | -2.20 | -3.30 | -3.66 | -1.23 | 0.90 | -1.33 | -2.82 | -2.51 | -1.38 | 0.23 |
Nuclear Periphery | -1.41 | -7.56 | -7.09 | -3.69 | 3.99 | -1.55 | -6.35 | -3.79 | 0.07 | 5.79 | -1.81 | -8.44 | -7.21 | -2.28 | 6.83 |
Nuclear Periphery Foci | -0.82 | -7.48 | -9.98 | -9.35 | 4.98 | -1.36 | -9.13 | -6.07 | -3.60 | 6.94 | -1.36 | -11.75 | -10.20 | -7.89 | 8.51 |
Nucleolus | -0.45 | -8.76 | 1.31 | 2.63 | 10.02 | -2.22 | -7.95 | -0.03 | 2.09 | 7.83 | -1.90 | -12.00 | 1.07 | 3.20 | 12.72 |
Nucleus | -4.13 | -8.60 | -10.06 | -8.10 | -4.27 | -1.52 | -5.91 | -7.85 | -7.06 | -2.74 | -3.57 | -9.98 | -12.43 | -10.48 | -5.07 |
Peroxisomes | -1.39 | -8.69 | -10.94 | -10.60 | -2.85 | -2.72 | -10.38 | -9.94 | -9.41 | -3.03 | -2.94 | -12.98 | -14.82 | -14.17 | -4.11 |
Vacuole | -1.32 | 30.75 | 38.26* | 40.25* | 10.07 | 7.47 | 45.12* | 47.45* | 35.24* | 7.17 | 6.08 | 54.67* | 62.00* | 53.57* | 12.31 |
Vacuole Periphery | 3.30 | 4.67 | 28.86* | 27.72* | 29.62* | 1.02 | -4.41 | 21.36 | 16.06 | 24.67* | 0.98 | -0.49 | 35.55 | 31.68* | 38.54* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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