Standard name | |
---|---|
Human Ortholog | |
Description | Alpha arrestin; controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 1 | 0 | 2 | 3 | 2 | 3 | 2 | 9 | 7 | 15 | 0 | 0 | 0 | 6 | 5 | 4 | 1 | 2 | 6 | 2 | 6 | 3 |
Bud | 0 | 1 | 1 | 1 | 5 | 6 | 22 | 23 | 18 | 27 | 34 | 22 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 3 | 2 | 5 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 7 | 26 | 31 | 14 | 26 | 16 | 20 | 27 | 39 | 38 | 48 | 12 | 23 | 30 | 33 | 28 | 36 | 0 | 0 | 5 | 1 | 2 | 2 |
Cytoplasm | 46 | 243 | 237 | 224 | 331 | 467 | 352 | 411 | 381 | 386 | 365 | 369 | 159 | 293 | 286 | 129 | 123 | 147 | 87 | 230 | 208 | 160 | 426 | 417 |
Endoplasmic Reticulum | 0 | 4 | 1 | 0 | 6 | 15 | 7 | 5 | 14 | 13 | 10 | 11 | 1 | 5 | 1 | 5 | 3 | 5 | 0 | 0 | 7 | 4 | 17 | 12 |
Endosome | 0 | 0 | 1 | 0 | 8 | 0 | 2 | 5 | 2 | 3 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 3 | 0 | 2 | 3 |
Golgi | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 3 | 2 |
Mitochondria | 0 | 7 | 5 | 16 | 34 | 165 | 214 | 218 | 283 | 269 | 307 | 263 | 3 | 0 | 1 | 6 | 2 | 2 | 2 | 3 | 3 | 5 | 9 | 9 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 5 | 1 | 3 | 2 | 2 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 3 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 1 | 0 | 3 | 5 | 8 | 8 | 22 | 24 | 19 | 5 | 23 | 0 | 0 | 0 | 0 | 2 | 6 | 2 | 7 | 1 | 4 | 13 | 5 |
Unique Cell Count | 48 | 253 | 246 | 234 | 363 | 580 | 517 | 585 | 579 | 615 | 586 | 583 | 164 | 298 | 287 | 148 | 138 | 163 | 99 | 259 | 244 | 185 | 497 | 471 |
Labelled Cell Count | 54 | 264 | 275 | 275 | 409 | 692 | 625 | 718 | 755 | 776 | 770 | 757 | 179 | 322 | 318 | 185 | 167 | 205 | 99 | 259 | 244 | 185 | 497 | 471 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 5.7 | 5.9 | 5.9 | 5.8 | 4.8 | 5.0 | 5.4 | 5.0 | 5.4 | 5.2 | 5.4 | 6.7 | 7.1 | 7.2 | 7.7 | 8.0 | 7.6 | 6.5 | 6.8 | 7.0 |
Std Deviation (1e-4) | 1.2 | 0.7 | 1.2 | 1.6 | 1.6 | 1.1 | 1.9 | 1.2 | 1.2 | 1.4 | 1.1 | 1.1 | 0.8 | 1.1 | 1.0 | 1.0 | 1.3 | 1.2 | 1.1 | 1.0 | 1.0 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.04 | -0.31 | -0.25 | -0.13 | -0.23 | -0.13 | -0.2 | -0.14 | 0.17 | 0.26 | 0.28 | 0.38 | 0.43 | 0.36 | 0.14 | 0.21 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 2.9 | 2.8 | 2.4 | 3.0 | 3.5 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.9 | -3.3 | 0 | -4.2 | -4.1 | -3.2 | -2.1 | -2.0 | -1.1 | -1.1 | -1.2 | 0 | 3.2 | 2.6 | 3.2 |
Cytoplasm | -0.3 | -2.5 | -5.9 | -8.7 | -8.3 | -9.3 | -10.0 | -10.0 | -9.8 | 0.3 | 1.5 | 2.8 | -3.4 | -2.8 | -2.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.6 | 3.6 | 0 | 11.2 | 10.5 | 12.9 | 11.9 | 13.7 | 12.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.1 | 3.2 | 2.8 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.177 | 1.674 | 1.4671 | 0.8457 | 0.6198 | 1.5289 | 2.5141 | 2.9802 | 2.7958 | 2.3897 | 2.5781 | 2.9422 | 1.5531 | 2.3059 | 1.8579 | 1.5961 | 1.5422 | 2.2126 |
Actin | 0.0063 | 0.0117 | 0.002 | 0.0078 | 0.1067 | 0.0522 | 0.0351 | 0.0007 | 0.0119 | 0.0171 | 0.0051 | 0.0182 | 0.0468 | 0.0014 | 0.011 | 0.0003 | 0.0039 | 0.0431 |
Bud | 0.0001 | 0.0008 | 0.0004 | 0.0004 | 0.0007 | 0.001 | 0.0009 | 0.0007 | 0.0032 | 0.003 | 0.0012 | 0.0008 | 0.0015 | 0.0001 | 0.0003 | 0.0001 | 0.001 | 0.0009 |
Bud Neck | 0.0007 | 0.0006 | 0.0026 | 0.0007 | 0.0024 | 0.005 | 0.0076 | 0.0005 | 0.0022 | 0.0014 | 0.0011 | 0.0104 | 0.0007 | 0.0001 | 0.001 | 0.0001 | 0.0004 | 0.002 |
Bud Periphery | 0.0001 | 0.0007 | 0.0007 | 0.0003 | 0.0009 | 0.0047 | 0.0014 | 0.0004 | 0.0033 | 0.0057 | 0.0035 | 0.0028 | 0.0026 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0013 |
Bud Site | 0.0012 | 0.0195 | 0.0039 | 0.0006 | 0.0022 | 0.001 | 0.0085 | 0.0099 | 0.0182 | 0.0062 | 0.0029 | 0.0013 | 0.0111 | 0.0019 | 0.0066 | 0.0001 | 0.0008 | 0.0022 |
Cell Periphery | 0.0025 | 0.0012 | 0.0006 | 0.0005 | 0.0008 | 0.004 | 0.0038 | 0.0006 | 0.0009 | 0.0005 | 0.0007 | 0.0017 | 0.0009 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0005 |
Cytoplasm | 0.8646 | 0.8816 | 0.9134 | 0.9109 | 0.8009 | 0.8313 | 0.7247 | 0.8516 | 0.8556 | 0.7639 | 0.8233 | 0.8547 | 0.7363 | 0.8694 | 0.8465 | 0.9518 | 0.7957 | 0.886 |
Cytoplasmic Foci | 0.0061 | 0.0094 | 0.0051 | 0.0121 | 0.019 | 0.017 | 0.0267 | 0.0095 | 0.0119 | 0.018 | 0.0312 | 0.0096 | 0.0116 | 0.0057 | 0.0151 | 0.0034 | 0.0307 | 0.0048 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0081 | 0.0037 | 0.0043 | 0.0074 | 0.0027 | 0.0056 | 0.0039 | 0.0018 | 0.0029 | 0.003 | 0.0026 | 0.0022 | 0.006 | 0.0046 | 0.0079 | 0.0046 | 0.0051 | 0.0048 |
Endosome | 0.0021 | 0.0065 | 0.0043 | 0.0067 | 0.0183 | 0.0023 | 0.0188 | 0.004 | 0.0065 | 0.0144 | 0.0229 | 0.0079 | 0.0059 | 0.0025 | 0.0043 | 0.0019 | 0.0939 | 0.0063 |
Golgi | 0.0009 | 0.0008 | 0.0006 | 0.002 | 0.0193 | 0.0015 | 0.0079 | 0.0013 | 0.0027 | 0.0032 | 0.0047 | 0.004 | 0.0107 | 0.0005 | 0.0054 | 0.0002 | 0.0434 | 0.0041 |
Lipid Particles | 0.0009 | 0.0002 | 0.0003 | 0.0004 | 0.0103 | 0.0012 | 0.0196 | 0.0005 | 0.002 | 0.001 | 0.005 | 0.005 | 0.0036 | 0.0008 | 0.0015 | 0.0003 | 0.0005 | 0.0023 |
Mitochondria | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0032 | 0.0003 | 0.0047 | 0.0005 | 0.0049 | 0.0041 | 0.011 | 0.0074 | 0.036 | 0 | 0.0054 | 0 | 0.0017 | 0.0013 |
None | 0.1017 | 0.0593 | 0.0584 | 0.046 | 0.0029 | 0.0705 | 0.1005 | 0.1149 | 0.063 | 0.1372 | 0.0654 | 0.0592 | 0.1177 | 0.1098 | 0.0718 | 0.0346 | 0.0174 | 0.0349 |
Nuclear Periphery | 0.0012 | 0.0005 | 0.0005 | 0.0007 | 0.0007 | 0.0003 | 0.0017 | 0.0002 | 0.0015 | 0.0008 | 0.0009 | 0.0019 | 0.0004 | 0.0006 | 0.0006 | 0.0005 | 0.0007 | 0.0018 |
Nucleolus | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0008 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Nucleus | 0.001 | 0.0007 | 0.0005 | 0.0006 | 0.0005 | 0.0004 | 0.0012 | 0.0006 | 0.0012 | 0.0021 | 0.001 | 0.0011 | 0.0005 | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0006 |
Peroxisomes | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0058 | 0.0004 | 0.0238 | 0.0003 | 0.0042 | 0.0122 | 0.0123 | 0.007 | 0.0053 | 0.0001 | 0.0197 | 0 | 0.0005 | 0.001 |
Punctate Nuclear | 0.0007 | 0.0004 | 0.0002 | 0.0003 | 0.0012 | 0.0004 | 0.005 | 0.0003 | 0.001 | 0.001 | 0.0024 | 0.0028 | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0012 |
Vacuole | 0.001 | 0.0016 | 0.0018 | 0.0019 | 0.0007 | 0.0007 | 0.0025 | 0.0013 | 0.0024 | 0.0037 | 0.0016 | 0.0012 | 0.0013 | 0.0007 | 0.0011 | 0.0012 | 0.0021 | 0.0005 |
Vacuole Periphery | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0005 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0011 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.1874 | 7.5368 | 16.7778 | 22.3763 | 10.0202 | 11.5862 | 15.0821 | 13.7154 | 14.5983 | 17.313 |
Translational Efficiency | 0.5459 | 0.8119 | 0.6574 | 0.5757 | 0.6954 | 0.8037 | 0.5925 | 0.6292 | 0.8692 | 0.5362 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1511 | 1668 | 3254 | 56 | 2261 | 2259 | 2596 | 1805 | 3772 | 3927 | 5850 | 1861 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 752.19 | 856.43 | 957.70 | 4500.69 | 806.16 | 802.46 | 867.77 | 943.68 | 784.54 | 825.38 | 917.79 | 1050.72 |
Standard Deviation | 88.40 | 117.34 | 115.58 | 1161.19 | 98.08 | 104.19 | 145.63 | 120.80 | 97.96 | 113.16 | 137.25 | 651.15 |
Intensity Change Log 2 | — | 0.187238 | 0.348477 | 2.580977 | — | -0.006637 | 0.106247 | 0.227232 | — | 0.090199 | 0.228249 | 1.804746 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000265 | 0.000838 | 0.000271 | 0.000338 | 0.000896 | 0.000854 | 0.001498 | 0.001407 | 0.000644 | 0.000847 | 0.000816 | 0.001375 |
Bud Neck | 0.012237 | 0.014987 | 0.005148 | 0.000858 | 0.016853 | 0.037315 | 0.007783 | 0.006824 | 0.015004 | 0.027831 | 0.006317 | 0.006645 |
Bud Site | 0.013833 | 0.029102 | 0.015297 | 0.000243 | 0.020835 | 0.043470 | 0.023647 | 0.038836 | 0.018030 | 0.037367 | 0.019002 | 0.037675 |
Cell Periphery | 0.000329 | 0.000249 | 0.000084 | 0.001341 | 0.000518 | 0.000333 | 0.000190 | 0.000209 | 0.000442 | 0.000297 | 0.000131 | 0.000243 |
Cytoplasm | 0.600332* | 0.611749* | 0.795536* | 0.206031* | 0.592555* | 0.528233* | 0.791663* | 0.642259* | 0.595670* | 0.563706* | 0.793817* | 0.629132* |
Cytoplasmic Foci | 0.211541* | 0.188164* | 0.018416 | 0.002657 | 0.193462* | 0.194486* | 0.034838 | 0.046229 | 0.200704* | 0.191801* | 0.025703 | 0.044918 |
Eisosomes | 0.000269 | 0.000209 | 0.000026 | 0.000012 | 0.000740 | 0.000405 | 0.000095 | 0.000075 | 0.000552 | 0.000322 | 0.000057 | 0.000073 |
Endoplasmic Reticulum | 0.001137 | 0.001075 | 0.000228 | 0.000047 | 0.001762 | 0.001010 | 0.000286 | 0.000771 | 0.001512 | 0.001038 | 0.000254 | 0.000749 |
Endosome | 0.016841 | 0.012381 | 0.001135 | 0.000007 | 0.013377 | 0.026723 | 0.001767 | 0.006694 | 0.014765 | 0.020631 | 0.001416 | 0.006493 |
Golgi | 0.005926 | 0.005803 | 0.000124 | 0.000244 | 0.010059 | 0.011840 | 0.000358 | 0.001626 | 0.008403 | 0.009276 | 0.000228 | 0.001585 |
Lipid Particles | 0.004897 | 0.002404 | 0.000317 | 0.000041 | 0.004741 | 0.002922 | 0.000562 | 0.000597 | 0.004803 | 0.002702 | 0.000426 | 0.000581 |
Mitochondria | 0.001798 | 0.001043 | 0.000221 | 0.001053 | 0.002137 | 0.004095 | 0.000276 | 0.003365 | 0.002001 | 0.002798 | 0.000246 | 0.003295 |
Mitotic Spindle | 0.000841 | 0.001842 | 0.004263 | 0.000000 | 0.000983 | 0.004637 | 0.001753 | 0.017202 | 0.000926 | 0.003450 | 0.003149 | 0.016685 |
None | 0.002782 | 0.001650 | 0.002205 | 0.030372 | 0.002328 | 0.000874 | 0.002599 | 0.001265 | 0.002510 | 0.001203 | 0.002380 | 0.002141 |
Nuclear Periphery | 0.000118 | 0.000074 | 0.000216 | 0.000139 | 0.000089 | 0.000124 | 0.000083 | 0.000129 | 0.000101 | 0.000103 | 0.000157 | 0.000129 |
Nuclear Periphery Foci | 0.000955 | 0.000750 | 0.000264 | 0.000207 | 0.000466 | 0.000211 | 0.000471 | 0.000510 | 0.000662 | 0.000440 | 0.000356 | 0.000501 |
Nucleolus | 0.000492 | 0.000266 | 0.000138 | 0.000330 | 0.000346 | 0.000300 | 0.000285 | 0.000248 | 0.000405 | 0.000286 | 0.000203 | 0.000251 |
Nucleus | 0.033520 | 0.036132 | 0.063818 | 0.752300* | 0.042927 | 0.022813 | 0.044233 | 0.023201 | 0.039159 | 0.028470 | 0.055127 | 0.045141 |
Peroxisomes | 0.002012 | 0.003379 | 0.000393 | 0.000007 | 0.002737 | 0.006819 | 0.001115 | 0.001991 | 0.002446 | 0.005358 | 0.000714 | 0.001931 |
Vacuole | 0.088348 | 0.086836 | 0.091800 | 0.003594 | 0.090637 | 0.110253 | 0.086306 | 0.205397* | 0.089720 | 0.100307 | 0.089362 | 0.199324 |
Vacuole Periphery | 0.001527 | 0.001067 | 0.000099 | 0.000180 | 0.001553 | 0.002281 | 0.000193 | 0.001163 | 0.001542 | 0.001765 | 0.000141 | 0.001134 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.95 | 1.06 | -1.32 | 3.62 | -1.79 | 0.16 | -1.16 | -0.34 | -0.68 | 0.87 | -1.12 | -0.29 | -1.25 | -0.66 | -1.22 |
Bud Neck | -2.04 | 9.06 | 14.06 | 12.78 | 7.57 | -11.41 | 11.03 | 11.99 | 19.23 | 1.48 | -10.77 | 14.72 | 13.24 | 19.99 | -0.27 |
Bud Site | -8.68 | 2.37 | 18.83* | 18.06* | 10.75 | -10.32 | 0.59 | -3.21 | 5.06 | -3.43 | -13.07 | 3.03 | -4.34 | 3.82 | -5.64 |
Cell Periphery | 4.28 | 16.44 | -1.85 | -1.97 | -2.23 | 5.15 | 10.84 | 10.46 | 5.16 | 0.27 | 6.44 | 17.37 | 6.86 | 2.80 | -2.71 |
Cytoplasm | -1.28 | -18.55 | 12.33 | 12.63 | 16.53 | 9.11 | -25.52 | -2.02 | -9.30 | 17.79 | 6.03 | -31.12 | -0.16 | -4.24 | 19.14 |
Cytoplasmic Foci | 3.95 | 45.06 | 48.99 | 43.05 | 16.44 | -0.20 | 42.46 | 38.35 | 35.80 | -3.48 | 2.32 | 62.84 | 49.34 | 44.64 | -8.50 |
Eisosomes | 2.93 | 14.36 | 14.86 | 14.90 | 3.43 | 4.18 | 8.56 | 8.93 | 12.49 | 1.34 | 4.73 | 10.80 | 10.59 | 15.07 | -1.38 |
Endoplasmic Reticulum | 0.28 | 5.45 | 7.10 | 6.02 | 13.42 | 2.62 | 6.49 | 3.27 | 1.27 | -0.79 | 2.45 | 8.24 | 3.01 | 1.55 | -0.83 |
Endosome | 3.63 | 15.84 | 16.94 | 16.30 | 17.86 | -10.27 | 21.00 | 12.07 | 17.33 | -7.91 | -6.36 | 25.84 | 15.01 | 18.26 | -8.71 |
Golgi | 0.32 | 18.31 | 16.87 | 12.42 | -1.39 | -2.04 | 18.11 | 15.20 | 15.06 | -3.22 | -1.51 | 23.55 | 17.47 | 16.72 | -3.68 |
Lipid Particles | 4.33 | 8.02 | 8.65 | 15.45 | 12.56 | 5.79 | 15.99 | 16.14 | 12.45 | 0.84 | 7.08 | 15.79 | 15.46 | 16.59 | -1.71 |
Mitochondria | 1.30 | 2.79 | 1.26 | -0.05 | -4.18 | -3.82 | 6.98 | -0.10 | 2.18 | -2.68 | -2.08 | 6.37 | -0.24 | 0.80 | -2.72 |
Mitotic Spindle | -1.01 | -3.07 | 1.27 | 2.49 | 5.58 | -3.49 | -1.13 | -6.03 | -3.66 | -5.76 | -3.46 | -3.43 | -6.07 | -4.47 | -5.05 |
None | 2.99 | 1.70 | -2.61 | -2.72 | -2.67 | 5.54 | 0.60 | 4.23 | -0.95 | 2.49 | 5.92 | 1.41 | 1.32 | -1.91 | 0.44 |
Nuclear Periphery | 1.38 | -8.29 | -0.72 | -2.04 | 6.22 | -0.50 | -0.87 | -2.87 | -0.70 | -4.76 | -0.05 | -7.76 | -3.12 | -1.71 | 7.50 |
Nuclear Periphery Foci | 1.43 | 8.64 | 4.66 | 2.63 | 0.48 | 8.56 | -1.76 | -0.42 | -6.31 | 1.09 | 3.49 | 6.26 | 3.86 | -0.51 | -1.11 |
Nucleolus | 5.23 | 7.81 | 1.56 | -1.06 | -2.22 | 1.90 | 2.28 | 1.41 | 0.75 | -0.06 | 5.36 | 7.64 | 2.26 | 0.51 | -0.70 |
Nucleus | -1.84 | -24.83 | -20.13 | -20.04 | -18.68 | 14.61 | -5.55 | 10.22 | -4.12 | 14.45 | 10.03 | -21.90 | -3.18 | -6.46 | 6.18 |
Peroxisomes | -5.85 | 14.01 | 18.91 | 16.36 | 5.79 | -9.71 | 9.01 | 3.24 | 11.30 | -3.44 | -11.02 | 14.83 | 2.79 | 11.17 | -5.18 |
Vacuole | 0.03 | -12.57 | 25.47 | 24.52 | 34.89* | -5.55 | -8.05 | -26.31 | -22.16 | -20.31 | -4.38 | -14.93 | -26.31 | -23.98 | -18.70 |
Vacuole Periphery | 2.18 | 8.03 | 6.64 | 5.85 | -1.14 | -3.23 | 10.51 | 2.70 | 4.77 | -2.38 | -1.38 | 13.34 | 2.90 | 3.64 | -2.55 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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