Standard name | |
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Human Ortholog | |
Description | Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 117 | 147 | 36 | 35 | 12 | 24 | 10 | 26 | – | 10 | 13 | 12 | 216 | 246 | 176 | 58 | 44 | 42 | 124 | 146 | 41 | 24 | 58 | 67 |
Bud | 76 | 102 | 40 | 21 | 33 | 35 | 28 | 39 | – | 33 | 26 | 40 | 97 | 88 | 64 | 46 | 31 | 20 | 161 | 181 | 62 | 102 | 172 | 130 |
Bud Neck | 5 | 13 | 2 | 4 | 1 | 1 | 5 | 4 | – | 2 | 3 | 10 | 4 | 0 | 2 | 8 | 24 | 4 | 77 | 136 | 30 | 67 | 48 | 35 |
Bud Site | 51 | 71 | 26 | 8 | 11 | 31 | 14 | 22 | – | 17 | 12 | 17 | 50 | 26 | 10 | 53 | 28 | 24 | – | – | – | – | – | – |
Cell Periphery | 30 | 16 | 4 | 6 | 3 | 1 | 3 | 7 | – | 7 | 27 | 17 | 45 | 44 | 54 | 6 | 2 | 4 | 12 | 8 | 2 | 1 | 3 | 4 |
Cytoplasm | 91 | 107 | 30 | 23 | 42 | 53 | 47 | 49 | – | 24 | 39 | 45 | 225 | 267 | 345 | 93 | 65 | 64 | 25 | 22 | 7 | 10 | 42 | 44 |
Endoplasmic Reticulum | 7 | 18 | 0 | 2 | 2 | 1 | 3 | 7 | – | 4 | 3 | 5 | 11 | 22 | 21 | 16 | 8 | 8 | 2 | 4 | 0 | 1 | 6 | 7 |
Endosome | 26 | 28 | 8 | 3 | 18 | 3 | 4 | 5 | – | 1 | 3 | 6 | 20 | 28 | 42 | 12 | 10 | 8 | 4 | 12 | 0 | 7 | 10 | 4 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 2 | 2 | 0 | 1 | 1 | 4 | 3 |
Mitochondria | 12 | 53 | 7 | 23 | 10 | 57 | 81 | 102 | – | 104 | 122 | 159 | 1 | 1 | 0 | 31 | 15 | 12 | 4 | 16 | 2 | 3 | 7 | 9 |
Nucleus | 11 | 11 | 5 | 0 | 5 | 2 | 2 | 6 | – | 1 | 4 | 2 | 6 | 4 | 0 | 11 | 6 | 5 | 0 | 2 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 8 | 11 | 3 | 0 | 3 | 3 | 7 | 4 | – | 3 | 5 | 8 | 2 | 0 | 2 | 4 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Peroxisomes | 40 | 41 | 29 | 23 | 23 | 21 | 24 | 27 | – | 24 | 10 | 22 | 38 | 40 | 41 | 40 | 33 | 27 | 2 | 3 | 2 | 3 | 7 | 0 |
SpindlePole | 48 | 33 | 37 | 12 | 13 | 8 | 17 | 22 | – | 19 | 11 | 22 | 160 | 95 | 93 | 84 | 49 | 31 | 12 | 13 | 5 | 6 | 10 | 6 |
Vac/Vac Membrane | 3 | 11 | 2 | 1 | 2 | 1 | 1 | 3 | – | 3 | 3 | 1 | 8 | 7 | 23 | 4 | 1 | 1 | 1 | 10 | 3 | 0 | 1 | 5 |
Unique Cell Count | 351 | 451 | 144 | 102 | 121 | 167 | 169 | 211 | 164 | 192 | 257 | 624 | 652 | 690 | 316 | 201 | 173 | 439 | 567 | 164 | 233 | 380 | 321 | |
Labelled Cell Count | 526 | 664 | 229 | 161 | 178 | 241 | 246 | 323 | 252 | 281 | 367 | 884 | 870 | 875 | 468 | 316 | 253 | 439 | 567 | 164 | 233 | 380 | 321 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 6.5 | 6.0 | 5.5 | 5.3 | 4.6 | 4.5 | 4.5 | – | 4.2 | 4.2 | 4.3 | 7.6 | 7.7 | 8.0 | 5.7 | 5.9 | 7.2 | 7.7 | 8.0 | 8.4 |
Std Deviation (1e-4) | 1.5 | 1.3 | 1.3 | 1.3 | 1.4 | 1.6 | 1.5 | 1.3 | – | 1.2 | 1.1 | 1.2 | 1.1 | 1.2 | 1.4 | 1.1 | 1.1 | 1.8 | 1.1 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.18 | -0.4 | -0.44 | -0.41 | – | -0.52 | -0.52 | -0.49 | 0.34 | 0.36 | 0.41 | -0.08 | -0.02 | 0.25 | 0.36 | 0.4 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.6 | -3.2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.2 | 2.9 | 0.1 | -1.6 | -0.7 | -0.1 |
Bud | -1.3 | -0.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | -3.5 | -4.2 | -6.1 | -3.4 | -2.8 | -3.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 |
Bud Site | -2.3 | -2.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | -3.6 | -6.2 | -8.9 | -0.3 | -1.0 | -1.0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.0 | 1.8 | 2.2 | 0 | 0 | 0 |
Cytoplasm | 0.3 | 2.5 | 2.2 | 1.4 | 0.5 | – | -1.4 | -0.1 | -0.8 | 3.5 | 4.5 | 6.4 | 1.9 | 2.4 | 3.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 |
Endosome | 0 | 2.5 | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.4 | -0.7 | 0.2 | -0.9 | -0.2 | -0.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.2 | 1.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 1.0 | 0.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.5 | -0.2 | 0 | 0 | 0 | – | 0 | 0 | 0 | -5.4 | -5.4 | -5.6 | -2.1 | -0.9 | -1.1 |
SpindlePole | -2.7 | -3.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | -3.2 | -3.7 | 0.2 | -0.3 | -1.7 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7718 | 3.0467 | 3.3051 | 2.7919 | 2.6975 | 3.4472 | 4.9652 | 4.7889 | 4.8465 | 4.8866 | 4.7611 | 5.3143 | 5.0532 | 4.3543 | 4.5082 | 4.4431 | 5.1356 | 4.5971 |
Actin | 0.1618 | 0.0941 | 0.0628 | 0.1802 | 0.2993 | 0.109 | 0.1971 | 0.0876 | 0.151 | 0.2673 | 0.4521 | 0.0983 | 0.1825 | 0.0898 | 0.1347 | 0.3371 | 0.3895 | 0.1342 |
Bud | 0.0328 | 0.0807 | 0.259 | 0.1518 | 0.1083 | 0.0112 | 0.0292 | 0.0663 | 0.2408 | 0.0734 | 0.0458 | 0.0123 | 0.0526 | 0.0775 | 0.2949 | 0.0706 | 0.0423 | 0.0131 |
Bud Neck | 0.0801 | 0.0078 | 0.0239 | 0.0016 | 0.004 | 0.6221 | 0.1321 | 0.0154 | 0.0251 | 0.0056 | 0.0266 | 0.6951 | 0.0815 | 0.0079 | 0.0132 | 0.0033 | 0.102 | 0.6552 |
Bud Periphery | 0.0858 | 0.0846 | 0.4226 | 0.5348 | 0.4586 | 0.0352 | 0.0762 | 0.071 | 0.3594 | 0.3314 | 0.2463 | 0.0274 | 0.0752 | 0.082 | 0.3697 | 0.2848 | 0.1564 | 0.0528 |
Bud Site | 0.2947 | 0.4633 | 0.0955 | 0.0256 | 0.0357 | 0.0353 | 0.2944 | 0.5139 | 0.1245 | 0.0607 | 0.0355 | 0.0195 | 0.2923 | 0.6233 | 0.098 | 0.0624 | 0.0545 | 0.0256 |
Cell Periphery | 0.014 | 0.0083 | 0.0018 | 0.0023 | 0.0065 | 0.0095 | 0.0134 | 0.0122 | 0.0065 | 0.005 | 0.0178 | 0.0055 | 0.0135 | 0.0064 | 0.0092 | 0.0079 | 0.0316 | 0.0062 |
Cytoplasm | 0.0699 | 0.1278 | 0.034 | 0.0419 | 0.0076 | 0.0663 | 0.0305 | 0.1064 | 0.0127 | 0.0959 | 0.0948 | 0.0527 | 0.058 | 0.062 | 0.0304 | 0.1213 | 0.0126 | 0.0298 |
Cytoplasmic Foci | 0.0754 | 0.024 | 0.016 | 0.004 | 0.0199 | 0.0413 | 0.0942 | 0.0313 | 0.0116 | 0.0159 | 0.0187 | 0.0459 | 0.0778 | 0.0065 | 0.0093 | 0.0135 | 0.0641 | 0.0406 |
Eisosomes | 0.0014 | 0.0008 | 0.0003 | 0.0006 | 0.0006 | 0.0004 | 0.0018 | 0.0009 | 0.0021 | 0.0017 | 0.0013 | 0.0003 | 0.0021 | 0.0002 | 0.0011 | 0.0009 | 0.0012 | 0.0004 |
Endoplasmic Reticulum | 0.0052 | 0.0018 | 0.0011 | 0.0024 | 0.0003 | 0.0009 | 0.0045 | 0.0015 | 0.0009 | 0.0049 | 0.0047 | 0.0004 | 0.0037 | 0.0002 | 0.0008 | 0.0009 | 0.0007 | 0.0004 |
Endosome | 0.0236 | 0.003 | 0.0027 | 0.0022 | 0.0013 | 0.0111 | 0.0175 | 0.0032 | 0.0043 | 0.0095 | 0.0019 | 0.0017 | 0.0345 | 0.0007 | 0.0037 | 0.0091 | 0.0082 | 0.0031 |
Golgi | 0.0178 | 0.0038 | 0.0016 | 0.0054 | 0.0037 | 0.0072 | 0.0106 | 0.0016 | 0.0025 | 0.006 | 0.0046 | 0.0023 | 0.0323 | 0.0011 | 0.0015 | 0.0174 | 0.0239 | 0.0044 |
Lipid Particles | 0.0152 | 0.0017 | 0.0016 | 0.0007 | 0.0008 | 0.0056 | 0.0091 | 0.0004 | 0.0019 | 0.0015 | 0.0015 | 0.0031 | 0.0148 | 0.0003 | 0.0056 | 0.0019 | 0.0168 | 0.0043 |
Mitochondria | 0.0114 | 0.0028 | 0.0149 | 0.0105 | 0.006 | 0.0036 | 0.0067 | 0.0012 | 0.0038 | 0.0032 | 0.0025 | 0.0006 | 0.0076 | 0.0005 | 0.0009 | 0.0345 | 0.0521 | 0.0103 |
None | 0.0318 | 0.0508 | 0.0285 | 0.0154 | 0.0097 | 0.0028 | 0.0259 | 0.0655 | 0.0337 | 0.1041 | 0.0072 | 0.0132 | 0.0079 | 0.0262 | 0.0185 | 0.0058 | 0.0031 | 0.0067 |
Nuclear Periphery | 0.0159 | 0.0008 | 0.0011 | 0.0027 | 0.0001 | 0.0014 | 0.0064 | 0.0059 | 0.0008 | 0.0013 | 0.0004 | 0.0002 | 0.0242 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0002 |
Nucleolus | 0.0046 | 0.0019 | 0.0002 | 0.0007 | 0.0005 | 0.001 | 0.0063 | 0.001 | 0.0025 | 0.0009 | 0.0011 | 0.0004 | 0.0006 | 0.0017 | 0.0005 | 0.0006 | 0.0006 | 0.0023 |
Nucleus | 0.0092 | 0.0045 | 0.0008 | 0.0031 | 0.0004 | 0.0011 | 0.003 | 0.002 | 0.0018 | 0.0019 | 0.0009 | 0.0004 | 0.0065 | 0.0021 | 0.0004 | 0.0013 | 0.0005 | 0.0007 |
Peroxisomes | 0.0196 | 0.0115 | 0.0294 | 0.0066 | 0.0341 | 0.0241 | 0.0164 | 0.0029 | 0.009 | 0.0043 | 0.0323 | 0.0168 | 0.0219 | 0.0068 | 0.0058 | 0.0192 | 0.0356 | 0.0051 |
Punctate Nuclear | 0.0203 | 0.0214 | 0.0009 | 0.0019 | 0.0004 | 0.0084 | 0.0212 | 0.0071 | 0.0025 | 0.0033 | 0.0009 | 0.0033 | 0.0065 | 0.0003 | 0.0007 | 0.0006 | 0.002 | 0.0024 |
Vacuole | 0.0075 | 0.0041 | 0.001 | 0.0048 | 0.0018 | 0.0021 | 0.0028 | 0.0019 | 0.0021 | 0.0021 | 0.0028 | 0.0007 | 0.0021 | 0.0042 | 0.0009 | 0.0059 | 0.0013 | 0.002 |
Vacuole Periphery | 0.0019 | 0.0005 | 0.0003 | 0.0009 | 0.0003 | 0.0004 | 0.0008 | 0.0009 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0021 | 0.0003 | 0.0001 | 0.0007 | 0.0004 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.2621 | 8.9575 | 10.9477 | 13.6358 | 12.6096 | 17.673 | 10.6552 | 16.1809 | 11.1679 | 17.0472 |
Translational Efficiency | 1.1037 | 0.9397 | 1.003 | 0.7614 | 0.9016 | 0.9303 | 0.9724 | 0.6689 | 1.3448 | 0.8867 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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