Standard name
Human Ortholog
Description Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.06 0.09 0.09 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.95 0.95 0.96 0.92 0.86 0.79 0.77 0.77 0.72 0.66 0.97 0.99 0.98 0.94 0.93 0.9 0.95 0.93 0.9 0.96 0.95 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.09 0.08 0.22 0.31 0.35 0.43 0.44 0.5 0.57 0 0 0.05 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 0 7 3 4 3 8 4 14 10 1 10 0 1 8 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 3 3 1 7 4 15 6 10 17 10 20 2 15 13 21 21 29 0 0 0 0 0 0
Cytoplasm 244 143 198 278 416 302 391 474 313 392 174 248 225 507 579 337 210 289 236 138 206 174 399 342
Endoplasmic Reticulum 1 1 1 5 1 0 1 4 2 1 3 3 2 3 3 11 10 6 0 0 2 0 4 6
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 2 0 0 1 0 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 3 3 0 0 0 0 2
Mitochondria 1 2 9 25 36 71 140 210 173 225 121 211 4 1 27 6 1 8 1 1 1 0 0 3
Nucleus 3 2 2 2 0 2 1 5 1 2 0 2 0 1 1 7 4 3 0 2 0 1 1 0
Nuclear Periphery 0 0 2 1 0 0 0 2 2 0 0 2 0 1 2 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 0 0 1 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 0 1 0 3 25 2 6 4 9 0 0 1 18 4 23 0 1 14 2 7 3
Unique Cell Count 247 146 208 293 434 327 455 601 407 512 240 373 231 512 590 360 225 320 248 149 230 182 420 366
Labelled Cell Count 257 151 224 315 467 382 561 732 518 657 313 506 233 529 634 402 254 362 248 149 230 182 420 366


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 7.5 5.7 5.8 5.6 4.8 4.3 4.3 3.9 4.0 4.0 3.7 8.4 8.4 6.7 11.5 12.9 13.3 9.1 8.8 9.0
Std Deviation (1e-4) 1.3 1.1 0.8 1.0 1.1 1.2 0.8 0.9 0.9 1.1 1.3 0.8 1.1 1.0 1.0 1.8 2.4 2.0 1.1 1.3 1.3
Intensity Change (Log2) – – – 0.04 -0.01 -0.25 -0.38 -0.4 -0.53 -0.52 -0.49 -0.62 0.57 0.56 0.23 1.02 1.19 1.23 0.67 0.63 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1.2 0 2.5 3.6 3.6
Cytoplasm -0.2 0.4 -1.3 -3.5 -5.4 -5.7 -5.9 -6.4 -7.9 1.2 3.3 2.3 -0.8 -0.8 -2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2.6 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.8 1.8 0 0 0 0 0 0 0 -1.6 0 0.1 -1.9 0 -1.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.6 0 0 0 0 0 0 0 2.9 0 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9796 6.7618 6.2788 6.2658 5.7508 6.1071 5.3292 8.0581 7.2572 6.42 6.2151 7.0886 4.3932 5.6208 5.3173 4.3439 3.7155 4.9507
Actin 0.0534 0.0007 0.0077 0.0003 0.0018 0.007 0.0336 0.0002 0.0073 0.0365 0.0002 0.0151 0.0445 0.0072 0.0008 0.0268 0.0023 0.0005
Bud 0.0006 0.0001 0.0004 0.0004 0.0002 0.0001 0.0019 0.0002 0.002 0.0005 0.0002 0.0003 0.0002 0.0002 0.0002 0.0003 0.0001 0.0001
Bud Neck 0.0148 0.0002 0.003 0.0006 0.0004 0.0014 0.0026 0.0002 0.0016 0.0003 0.0003 0.0006 0.0005 0.0008 0.0002 0.0052 0.0023 0.0006
Bud Periphery 0.0008 0.0001 0.0004 0.0002 0.0002 0.0001 0.0012 0.0001 0.0009 0.0006 0.0002 0.0003 0.0002 0.0002 0 0.0004 0.0001 0
Bud Site 0.0042 0.0006 0.0029 0.0002 0.0004 0.0001 0.0087 0.0011 0.0107 0.0012 0.0001 0.0002 0.0005 0.0012 0.0003 0.001 0.0004 0.0001
Cell Periphery 0.0004 0.0004 0.0002 0.0001 0.0001 0.0001 0.0005 0 0.0001 0.0002 0 0 0.0003 0.0004 0.0001 0.0005 0.0002 0.0001
Cytoplasm 0.7268 0.9794 0.9365 0.9532 0.9326 0.9625 0.7678 0.9838 0.9266 0.8364 0.8983 0.9335 0.8386 0.841 0.9722 0.7532 0.6149 0.9223
Cytoplasmic Foci 0.0228 0.0017 0.0131 0.0065 0.0084 0.0049 0.0349 0.003 0.0098 0.0174 0.0066 0.005 0.0052 0.008 0.0048 0.0268 0.0096 0.0067
Eisosomes 0.0006 0 0.0001 0 0 0.0002 0.0003 0 0.0001 0.0002 0 0 0.0007 0.0001 0 0.001 0.0004 0
Endoplasmic Reticulum 0.0171 0.0075 0.0074 0.0018 0.0049 0.0024 0.0159 0.0013 0.0019 0.0129 0.0011 0.0018 0.008 0.019 0.005 0.0333 0.0066 0.0055
Endosome 0.0446 0.002 0.0124 0.0273 0.0116 0.0063 0.0303 0.0018 0.0061 0.0289 0.0623 0.0127 0.0024 0.015 0.0044 0.0184 0.0153 0.008
Golgi 0.0098 0.0004 0.0017 0.0022 0.0091 0.0052 0.0069 0.0002 0.0017 0.0105 0.0112 0.0068 0.003 0.0039 0.0013 0.0029 0.0024 0.0086
Lipid Particles 0.0154 0.0001 0.0015 0 0.0019 0.0007 0.0075 0 0.0022 0.0082 0.0004 0.0034 0.0042 0.0094 0.0001 0.0087 0.0019 0.0002
Mitochondria 0.0106 0.0001 0.0008 0.001 0.02 0.0008 0.0043 0.0001 0.0143 0.0026 0.0072 0.011 0.0005 0.0005 0.0002 0.0018 0.1268 0.0037
None 0.0175 0.0025 0.0033 0.001 0.0012 0.0021 0.0269 0.0059 0.0037 0.0099 0.0013 0.0023 0.083 0.0077 0.0087 0.0287 0.0964 0.0414
Nuclear Periphery 0.0182 0.0015 0.001 0.0004 0.0006 0.0006 0.0201 0.0002 0.0006 0.0044 0.0006 0.0013 0.001 0.0241 0.0004 0.0283 0.0151 0.0004
Nucleolus 0.0014 0 0.0001 0 0 0 0.0009 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0004 0 0.0009 0.0077 0
Nucleus 0.0118 0.0013 0.0012 0.0007 0.001 0.0028 0.0044 0.0008 0.0007 0.0011 0.0005 0.0007 0.0009 0.0484 0.0005 0.0376 0.0737 0.0008
Peroxisomes 0.0052 0 0.0023 0 0.0036 0.0001 0.0105 0 0.0074 0.0225 0.0001 0.002 0.0042 0.0015 0 0.0077 0.0132 0.0001
Punctate Nuclear 0.0182 0.0001 0.0003 0 0.0001 0.0002 0.0144 0.0001 0.0013 0.0014 0 0.0018 0.0015 0.0056 0.0001 0.0072 0.0071 0.0002
Vacuole 0.0042 0.0013 0.0031 0.0027 0.0014 0.0019 0.0041 0.0009 0.0008 0.003 0.0054 0.0006 0.0006 0.0049 0.0007 0.0081 0.0027 0.0006
Vacuole Periphery 0.0016 0.0001 0.0007 0.0016 0.0004 0.0004 0.0022 0.0001 0.0002 0.0009 0.0042 0.0004 0.0001 0.0008 0.0001 0.0011 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.9585 20.8018 25.112 21.8263 35.1236 23.9511 18.7567 15.0431 16.2578 29.2482
Translational Efficiency 1.2449 1.2355 0.8592 0.7152 0.6803 0.8872 0.9089 0.6991 0.6687 0.7025

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
897 1200 2222 1188 1529 1742 944 115 2426 2942 3166 1303

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 886.05 834.65 1150.45 1108.64 856.31 944.68 1124.51 1157.01 867.31 899.80 1142.72 1112.91
Standard Deviation 100.11 158.73 132.73 133.65 143.11 133.44 140.64 148.58 129.69 154.09 135.66 135.73
Intensity Change Log 2 — -0.086217 0.376738 0.323331 — 0.141693 0.393091 0.434196 — 0.030291 0.384798 0.378882

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000152 0.000177 0.000103 0.000987 0.000118 0.000259 0.000176 0.000372 0.000131 0.000225 0.000124 0.000932
Bud Neck 0.001084 0.002874 0.001390 0.002490 0.001771 0.003420 0.001635 0.001426 0.001517 0.003198 0.001463 0.002396
Bud Site 0.006840 0.012851 0.009451 0.025535 0.006750 0.013396 0.009266 0.025168 0.006783 0.013173 0.009396 0.025502
Cell Periphery 0.000137 0.000090 0.000048 0.000088 0.000089 0.000126 0.000096 0.000202 0.000106 0.000112 0.000062 0.000098
Cytoplasm 0.773822* 0.767014* 0.835149* 0.689076* 0.788188* 0.690762* 0.640820* 0.732607* 0.782877* 0.721864* 0.777206* 0.692918*
Cytoplasmic Foci 0.115775 0.095060 0.024999 0.067128 0.103261 0.136987 0.052850 0.112113 0.107888 0.119885 0.033303 0.071098
Eisosomes 0.000029 0.000018 0.000007 0.000014 0.000015 0.000028 0.000031 0.000016 0.000020 0.000024 0.000014 0.000014
Endoplasmic Reticulum 0.001041 0.000909 0.000395 0.000721 0.002288 0.000762 0.001109 0.000145 0.001827 0.000822 0.000608 0.000670
Endosome 0.005514 0.010411 0.001748 0.011088 0.006058 0.012728 0.006691 0.003269 0.005857 0.011783 0.003222 0.010398
Golgi 0.001873 0.002869 0.000136 0.012181 0.002714 0.004050 0.000180 0.000294 0.002403 0.003569 0.000149 0.011132
Lipid Particles 0.001199 0.001574 0.000401 0.000597 0.001147 0.001598 0.001290 0.001341 0.001166 0.001588 0.000666 0.000662
Mitochondria 0.000208 0.000688 0.000087 0.004978 0.000346 0.001816 0.000115 0.000006 0.000295 0.001356 0.000096 0.004539
Mitotic Spindle 0.002758 0.001610 0.003034 0.023403 0.001047 0.004634 0.002810 0.000249 0.001679 0.003401 0.002967 0.021360
None 0.004197 0.006034 0.003334 0.000955 0.006944 0.002561 0.002812 0.000466 0.005928 0.003977 0.003178 0.000912
Nuclear Periphery 0.000098 0.000296 0.000206 0.000113 0.000155 0.000099 0.000387 0.000056 0.000134 0.000179 0.000260 0.000108
Nuclear Periphery Foci 0.002438 0.001940 0.002004 0.001450 0.001488 0.000863 0.004479 0.003411 0.001840 0.001303 0.002742 0.001623
Nucleolus 0.000454 0.000737 0.000120 0.000113 0.000481 0.000665 0.000240 0.000053 0.000471 0.000694 0.000156 0.000108
Nucleus 0.046355 0.030379 0.058211 0.012291 0.033950 0.023040 0.063987 0.006363 0.038537 0.026034 0.059933 0.011768
Peroxisomes 0.000567 0.001103 0.000138 0.003044 0.000535 0.002042 0.000437 0.001092 0.000547 0.001659 0.000227 0.002872
Vacuole 0.034730 0.060292 0.058866 0.141299 0.041434 0.098334 0.210305* 0.111254 0.038956 0.082817 0.104020 0.138648
Vacuole Periphery 0.000729 0.003075 0.000174 0.002448 0.001221 0.001830 0.000284 0.000097 0.001039 0.002338 0.000207 0.002241

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.96 0.79 -3.04 -2.85 -3.19 -3.21 -3.32 -3.84 -1.05 -2.16 -3.39 -1.39 -3.40 -2.50 -3.17
Bud Neck -4.20 -1.20 -2.40 2.23 -0.90 -4.66 0.54 1.76 6.45 1.40 -6.10 0.15 -0.73 4.27 -0.77
Bud Site -4.41 -1.37 -6.42 -2.59 -4.68 -5.15 -1.18 -5.55 -2.45 -4.55 -6.80 -1.86 -7.76 -2.89 -5.66
Cell Periphery 3.64 8.45 6.49 2.63 -2.52 -0.76 0.34 -1.11 -0.12 -1.15 -0.14 9.13 4.97 1.39 -1.56
Cytoplasm 0.95 -3.05 13.29 12.11 17.37 12.55 18.49 6.15 2.05 -1.59 10.42 7.85 16.89 9.28 11.37
Cytoplasmic Foci 3.61 19.92 12.54 9.20 -11.16 -6.51 14.18 1.32 4.42 -4.21 -3.01 26.38 14.38 17.42 -10.13
Eisosomes 4.10 10.62 7.64 2.78 -7.70 -8.08 -9.18 -1.33 4.92 5.54 -2.92 5.74 6.07 8.26 0.61
Endoplasmic Reticulum 0.51 2.98 2.82 2.50 0.54 2.17 1.80 3.12 3.93 12.09 2.21 2.74 3.25 2.84 2.19
Endosome -4.14 5.91 0.20 3.99 -4.50 -7.91 1.17 4.26 11.16 3.79 -8.53 7.16 0.95 7.31 -3.11
Golgi -1.38 6.94 -2.16 -1.31 -3.56 -2.10 16.58 15.66 6.35 -1.05 -2.44 16.84 -1.61 -0.62 -3.56
Lipid Particles -1.43 6.18 4.29 3.72 -4.30 -1.66 -0.61 -0.62 0.45 -0.44 -2.18 5.72 4.60 4.56 -0.80
Mitochondria -2.03 2.36 -1.99 -1.41 -2.13 -2.62 5.01 9.76 3.25 2.79 -3.06 6.40 -1.86 -0.48 -2.12
Mitotic Spindle 0.63 -0.12 -3.62 -4.78 -4.23 -2.81 -2.42 1.28 3.54 3.67 -1.74 -1.62 -4.73 -3.77 -4.13
None -1.37 2.14 5.83 4.66 7.87 4.59 5.51 8.41 3.18 3.77 2.59 5.90 9.99 5.52 8.27
Nuclear Periphery -1.80 -6.75 -3.27 0.98 5.95 0.83 -5.00 0.62 -0.74 14.32 -0.76 -5.02 -1.15 -0.07 11.02
Nuclear Periphery Foci 1.19 0.21 2.87 2.27 4.85 3.02 -9.71 -5.28 -7.61 2.75 2.62 -5.55 0.96 -2.55 8.46
Nucleolus -1.04 1.46 1.55 6.84 2.69 -0.99 3.71 5.88 2.97 6.38 -1.45 3.32 3.80 4.56 5.09
Nucleus 4.92 -6.73 10.96 6.61 28.74 5.89 -10.18 11.93 8.22 17.61 7.35 -14.69 14.25 8.06 31.85
Peroxisomes -2.33 2.26 -2.13 -0.98 -3.63 -5.52 2.02 -1.33 3.91 -2.18 -6.06 4.21 -2.51 0.71 -3.61
Vacuole -8.29 -13.99 -27.82 -23.30 -19.69 -15.51 -25.40 -8.31 -5.66 1.05 -17.27 -25.62 -28.93 -21.55 -13.41
Vacuole Periphery -3.09 3.80 -0.77 2.59 -2.79 -2.43 7.80 9.03 8.34 4.41 -3.78 8.86 0.46 3.32 -2.69
Standard name
Human Ortholog
Description Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Sap185

Sap185


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sap185-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available