Standard name
Human Ortholog
Description Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0.07 0.09 0.08 0.08 0.11 0.1 0.09 0.1 0.05 0.05 0 0.06 0.05 0.17 0.09 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.06 0.1 0.08 0.08 0.07 0.08 0.12 0.08 0.09 0.1 0.17 0.23 0.34 0.26 0.26 0 0 0 0 0 0
Cytoplasm 0.28 0.07 0 0.07 0.08 0.07 0.08 0.09 0.09 0.09 0.06 0.05 0.05 0.08 0.24 0.24 0.26 0 0 0 0 0 0
Endoplasmic Reticulum 0.59 0.86 0.89 0.75 0.76 0.77 0.7 0.69 0.6 0.53 0.65 0.85 0.88 0.8 0 0 0 0.76 0.91 0.93 0.91 0.85 0.86
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.07 0 0.07 0.19 0.08 0 0 0 0 0 0 0 0 0 0.34 0.48 0.33 0 0 0 0 0 0
Mitochondria 0 0 0.14 0.05 0.11 0.16 0.17 0.22 0.19 0.34 0.3 0 0 0 0.07 0.07 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.05 0.09 0.07 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.1 0 0.13 0.08 0.05 0.12 0 0.11 0.08 0.05 0.08 0 0.05 0.16 0.05 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 20 14 14 29 22 32 39 34 27 39 13 19 10 7 4 8 10 9 2 1 8 4
Bud 1 0 2 1 1 0 5 9 6 6 5 1 1 0 0 0 0 1 7 1 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 35 12 20 26 21 30 27 41 24 36 23 64 87 41 23 12 0 3 1 0 2 5
Cytoplasm 29 46 9 14 24 18 33 30 28 27 22 13 19 31 29 21 12 2 8 0 2 5 3
Endoplasmic Reticulum 61 549 192 150 243 212 292 241 196 155 256 201 340 302 2 0 0 88 614 213 212 244 281
Endosome 0 0 0 1 1 1 0 1 2 0 0 0 0 0 0 0 0 4 2 0 1 2 0
Golgi 7 26 15 38 25 4 5 1 2 2 7 10 13 8 42 42 15 0 2 2 2 6 2
Mitochondria 0 2 31 10 34 43 71 75 63 98 119 0 0 1 8 6 3 2 2 4 3 4 6
Nucleus 2 0 0 0 3 3 0 1 3 1 1 0 1 0 0 0 0 0 2 0 0 0 0
Nuclear Periphery 2 3 6 4 6 12 22 30 23 19 31 1 3 4 0 3 0 1 3 1 1 2 1
Nucleolus 5 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0
Vac/Vac Membrane 7 64 8 26 26 13 49 15 36 24 19 19 14 20 19 4 7 0 8 1 3 6 13
Unique Cell Count 103 639 215 201 318 276 417 348 328 291 393 237 386 377 122 88 46 117 676 231 234 288 326
Labelled Cell Count 117 745 290 278 418 349 539 469 434 383 536 281 474 463 148 103 57 117 676 231 234 288 326


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.7 25.9 19.3 20.0 17.4 14.9 14.2 14.0 13.8 13.6 14.0 26.7 26.1 25.5 28.7 26.9 29.1 26.3 25.1 28.2
Std Deviation (1e-4) 10.1 8.4 6.4 6.1 5.1 4.0 3.2 3.5 3.6 3.4 3.5 10.0 8.0 8.1 10.1 8.7 11.6 9.1 8.3 9.7
Intensity Change (Log2) 0.05 -0.15 -0.37 -0.44 -0.46 -0.48 -0.51 -0.46 0.47 0.44 0.41 0.58 0.48 0.6 0.45 0.38 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.2 1.1 0.6 0.5 1.9 1.5 1.1 1.4 -0.5 -0.8 -2.3 -0.3 -0.7 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.7 1.1 0.9 0.8 1.0 2.7 1.2 1.6 1.6 3.9 5.5 6.8 5.1 0
Cytoplasm 1.2 1.6 1.1 1.8 2.0 2.0 2.2 0.8 0.6 0.4 1.9 5.5 5.2 0
Endoplasmic Reticulum -3.9 -3.8 -3.6 -5.4 -5.5 -7.5 -8.6 -6.5 -1.4 -0.4 -2.9 -15.6 -14.6 -12.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.6 0.4 -3.2 -3.9 -4.6 -4.2 -3.9 -3.3 -1.3 -2.0 -2.9 6.5 8.2 4.9
Mitochondria -3.2 -1.3 0.4 0.8 2.1 1.4 4.9 4.3 -6.1 -7.7 -7.3 -2.2 -1.8 -1.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.9 1.4 2.7 2.1 1.9 2.5 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.4 2.1 0.5 3.3 0.3 3.0 2.1 0.6 1.9 -0.1 0.9 3.9 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.4505 17.9481 16.0681 14.046 14.2893 15.7962 22.911 22.4968 22.0767 18.9942 20.2072 21.408 22.8188 19.3027 19.7992 19.3829 19.3378 19.7252
Actin 0.0058 0.0005 0.0047 0.005 0.0002 0.0048 0.0021 0.0003 0.0003 0 0.0027 0.001 0.0001 0.0007 0 0 0 0.0001
Bud 0.0001 0 0.0002 0.0002 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.0005 0.0006 0.0003 0.0002 0.0003 0.0003 0.0001 0.0001 0.0003 0 0.0002 0.0001 0 0.0002 0 0.0001 0 0.0006
Bud Periphery 0.0002 0.0001 0.0003 0.0002 0.0001 0.0002 0.0001 0 0.0001 0 0 0 0 0.0002 0 0 0 0
Bud Site 0.0009 0.0002 0.0034 0.001 0.0002 0.0001 0.0002 0 0 0 0 0 0 0.0005 0 0 0 0
Cell Periphery 0.0025 0.0057 0.0046 0.0018 0.0013 0.0008 0.0015 0.0009 0.0025 0.0002 0.0005 0.0004 0.0011 0.0051 0.0016 0.0018 0.0007 0.0008
Cytoplasm 0.0126 0.0132 0.0057 0.0024 0.0021 0.0053 0.0023 0.0067 0.002 0.0002 0.0055 0.0013 0.0067 0.0307 0.0031 0.001 0.0013 0.0042
Cytoplasmic Foci 0.0062 0.0007 0.0006 0.0038 0.0038 0.0009 0.005 0.0001 0.0001 0 0.0011 0.0007 0.0001 0.0015 0.0001 0 0 0
Eisosomes 0.0004 0.0001 0.0004 0.0001 0 0.0001 0.0001 0 0 0 0.0001 0 0 0.0001 0 0 0 0
Endoplasmic Reticulum 0.8598 0.941 0.9299 0.9002 0.9494 0.9499 0.9288 0.9827 0.9831 0.9972 0.9381 0.9889 0.9418 0.9138 0.9865 0.9905 0.9881 0.9751
Endosome 0.0099 0.0016 0.0082 0.0222 0.0078 0.0057 0.004 0.0005 0.0008 0.0001 0.0052 0.0015 0.0004 0.0027 0.0002 0.0002 0.0004 0.0007
Golgi 0.004 0.0004 0.0057 0.0055 0.0014 0.0043 0.0013 0.0002 0.0043 0 0.0152 0.0005 0.0001 0.0006 0.0001 0.0001 0.0001 0.0003
Lipid Particles 0.0128 0.0112 0.0023 0.0066 0.0136 0.0028 0.0052 0.002 0.0009 0 0.0107 0.0002 0.0011 0.0129 0.0013 0.0002 0.0007 0.0003
Mitochondria 0.0091 0.0002 0.0084 0.0103 0.0004 0.002 0.0011 0.0001 0.0001 0 0.0003 0.0001 0.0002 0.0001 0 0 0 0
None 0.0019 0.0008 0.0002 0.0005 0.0003 0.0004 0.0016 0 0 0 0.0004 0.0001 0 0.0002 0 0 0 0
Nuclear Periphery 0.0507 0.012 0.0167 0.0325 0.0104 0.0168 0.0359 0.005 0.0042 0.0017 0.0146 0.0043 0.0429 0.0232 0.0056 0.0046 0.0071 0.0129
Nucleolus 0.0004 0.0002 0.0001 0.0002 0.0004 0 0 0 0 0 0.0001 0 0 0 0 0 0 0
Nucleus 0.0046 0.0034 0.0009 0.0012 0.0006 0.0019 0.0036 0.0002 0.0001 0 0.0024 0.0001 0.003 0.0029 0.0001 0.0002 0.0002 0.0019
Peroxisomes 0.0067 0 0.0001 0.0007 0.0025 0.0002 0.0015 0 0 0 0 0.0001 0 0 0 0 0 0
Punctate Nuclear 0.0019 0.0008 0.0001 0.0006 0.0027 0.0005 0.0007 0 0 0 0.0013 0.0001 0 0.0003 0 0 0 0
Vacuole 0.0062 0.007 0.0047 0.0027 0.0024 0.0023 0.0029 0.001 0.001 0.0005 0.0011 0.0006 0.0017 0.004 0.0011 0.0011 0.0013 0.0027
Vacuole Periphery 0.0029 0.0003 0.0027 0.0021 0.0003 0.0006 0.0019 0.0002 0.0002 0 0.0005 0.0001 0.0007 0.0004 0.0001 0.0002 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 116.7062 112.7588 143.8844 159.9761 116.925 130.78 141.181 158.5731 154.849 160.6324
Translational Efficiency 1.2495 1.4528 1.352 1.2485 1.1844 1.2939 1.3394 1.1037 1.3598 1.1258

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2152 1878 2117 1754 1697 1575 2237 1543 3849 3453 4354 3297

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2350.74 2060.65 2163.60 2609.02 2196.64 1538.23 2234.15 2593.50 2282.80 1822.36 2199.85 2601.76
Standard Deviation 423.75 531.54 393.63 531.64 416.46 347.87 382.87 514.36 427.45 525.90 389.73 523.68
Intensity Change Log 2 -0.190015 -0.119681 0.150393 -0.514027 0.024428 0.239602 -0.337488 -0.048270 0.194175

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000483 0.000579 0.000333 0.001752 0.000687 0.001324 0.000778 0.001024 0.000573 0.000919 0.000561 0.001412
Bud Neck 0.000157 0.000167 0.000715 0.005243 0.000278 0.000223 0.000706 0.003398 0.000210 0.000193 0.000711 0.004380
Bud Site 0.000544 0.000866 0.001416 0.013427 0.000436 0.002456 0.002566 0.008907 0.000497 0.001591 0.002007 0.011311
Cell Periphery 0.002147 0.002041 0.004119 0.010844 0.003643 0.002931 0.005394 0.014440 0.002807 0.002447 0.004774 0.012527
Cytoplasm 0.000212 0.000891 0.001757 0.009183 0.000537 0.002735 0.003160 0.004091 0.000356 0.001732 0.002478 0.006800
Cytoplasmic Foci 0.000357 0.000263 0.000116 0.002891 0.000167 0.000700 0.000686 0.001470 0.000274 0.000462 0.000409 0.002226
Eisosomes 0.000284 0.000087 0.000034 0.000249 0.000698 0.000163 0.000049 0.000185 0.000467 0.000122 0.000041 0.000219
Endoplasmic Reticulum 0.985732* 0.983160* 0.970830* 0.831516* 0.976958* 0.968746* 0.958721* 0.867278* 0.981864* 0.976585* 0.964609* 0.848253*
Endosome 0.001954 0.003334 0.003989 0.017915 0.003068 0.006672 0.004780 0.014413 0.002445 0.004856 0.004395 0.016276
Golgi 0.002203 0.001137 0.001270 0.010198 0.004871 0.001967 0.003154 0.008571 0.003379 0.001515 0.002238 0.009436
Lipid Particles 0.001488 0.001394 0.003328 0.031429 0.001677 0.004184 0.005175 0.025187 0.001572 0.002666 0.004277 0.028508
Mitochondria 0.000312 0.000469 0.000251 0.001973 0.000765 0.000445 0.000808 0.001889 0.000512 0.000458 0.000537 0.001934
Mitotic Spindle 0.000013 0.000223 0.000055 0.003292 0.000026 0.000137 0.000400 0.000823 0.000019 0.000184 0.000232 0.002137
None 0.000304 0.000773 0.000398 0.002506 0.000178 0.000897 0.001070 0.002102 0.000248 0.000829 0.000743 0.002317
Nuclear Periphery 0.000870 0.000683 0.002101 0.004112 0.001052 0.000869 0.001523 0.003459 0.000950 0.000768 0.001804 0.003806
Nuclear Periphery Foci 0.000496 0.001669 0.004178 0.027828 0.001202 0.003722 0.003601 0.016292 0.000807 0.002605 0.003881 0.022429
Nucleolus 0.000016 0.000017 0.000005 0.000047 0.000006 0.000005 0.000008 0.000028 0.000011 0.000012 0.000007 0.000038
Nucleus 0.000357 0.000085 0.000116 0.001107 0.000049 0.000114 0.000132 0.000561 0.000221 0.000098 0.000124 0.000852
Peroxisomes 0.000048 0.000056 0.000023 0.000095 0.000035 0.000149 0.000102 0.000060 0.000042 0.000098 0.000064 0.000079
Vacuole 0.001261 0.001668 0.004280 0.019610 0.002247 0.001181 0.005802 0.020372 0.001696 0.001446 0.005062 0.019966
Vacuole Periphery 0.000761 0.000438 0.000686 0.004783 0.001419 0.000381 0.001386 0.005449 0.001051 0.000412 0.001046 0.005095

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.25 0.10 -3.60 -3.18 -3.90 -1.06 -0.23 -1.14 0.10 -0.99 -1.04 -0.23 -3.32 -2.30 -3.24
Bud Neck -0.06 -3.81 -8.72 -8.73 -7.57 0.65 -2.35 -7.14 -7.33 -5.65 0.40 -4.22 -11.18 -11.29 -9.41
Bud Site -0.77 -2.33 -9.11 -8.87 -8.12 -3.07 -3.84 -6.75 -4.94 -4.64 -2.92 -4.39 -11.31 -9.95 -9.10
Cell Periphery 0.30 -4.17 -8.65 -8.70 -6.34 1.21 -2.48 -7.78 -8.15 -6.12 1.10 -4.65 -11.59 -11.86 -8.66
Cytoplasm -3.32 -6.89 -8.52 -7.83 -6.92 -2.63 -3.35 -4.30 -2.21 -1.49 -3.60 -5.49 -9.45 -7.59 -6.19
Cytoplasmic Foci 0.46 0.90 -4.21 -5.23 -5.57 -3.21 -3.29 -3.84 -2.09 -2.10 -0.76 -0.55 -5.40 -5.54 -5.70
Eisosomes 1.30 1.66 0.19 -1.52 -2.04 1.45 1.77 1.38 -0.41 -3.39 1.88 2.33 1.28 -1.55 -3.03
Endoplasmic Reticulum 1.27 6.69 23.42 22.70 20.31 2.39 5.83 17.20 15.51 13.58 2.73 9.03 28.99 27.29 24.02
Endosome -4.37 -6.85 -19.18 -16.78 -15.97 -5.67 -4.27 -14.32 -8.21 -11.39 -7.16 -7.84 -23.94 -17.86 -19.41
Golgi 4.51 3.60 -6.86 -7.83 -7.67 4.22 2.30 -3.88 -6.59 -5.23 5.53 2.91 -7.95 -10.25 -9.06
Lipid Particles 0.13 -2.90 -12.02 -12.08 -11.02 -2.71 -4.57 -10.36 -8.74 -8.41 -2.34 -5.36 -15.85 -14.89 -13.78
Mitochondria -0.93 0.43 -3.97 -3.57 -4.17 0.80 -0.17 -1.82 -2.83 -2.01 0.32 -0.23 -3.92 -4.51 -4.15
Mitotic Spindle -1.27 -3.49 -5.05 -4.61 -4.96 -1.26 -2.00 -3.74 -3.06 -1.47 -1.66 -2.28 -5.87 -5.28 -5.08
None -1.85 -0.75 -4.49 -3.30 -4.36 -3.20 -3.65 -2.83 -1.70 -1.40 -3.37 -3.45 -5.03 -3.43 -3.66
Nuclear Periphery 1.26 -4.11 -9.54 -9.54 -4.36 1.03 -2.78 -7.57 -7.64 -5.64 1.59 -5.05 -12.21 -12.23 -6.98
Nuclear Periphery Foci -2.98 -5.75 -13.63 -12.75 -11.07 -4.22 -4.06 -8.85 -7.21 -7.08 -5.17 -7.13 -16.19 -14.49 -13.13
Nucleolus 0.11 1.00 -2.18 -2.42 -5.73 0.20 -1.44 -4.59 -4.44 -4.06 0.15 0.82 -3.33 -3.70 -6.99
Nucleus 1.04 0.69 -2.43 -5.72 -5.07 -1.69 -3.38 -4.68 -4.26 -4.07 0.89 0.47 -3.72 -7.12 -6.49
Peroxisomes -0.27 1.03 -1.51 -1.55 -3.38 -1.92 -2.48 -1.48 1.53 1.66 -1.79 -1.11 -1.93 0.66 -0.87
Vacuole -1.08 -6.73 -12.79 -12.18 -10.21 1.87 -4.68 -9.72 -10.62 -7.62 0.67 -7.80 -15.78 -16.05 -12.39
Vacuole Periphery 1.80 0.33 -6.46 -7.09 -6.64 4.80 -0.10 -5.24 -6.77 -4.94 4.58 -0.16 -8.28 -9.79 -7.95
Standard name
Human Ortholog
Description Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Pho86

Pho86


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pho86-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available