Standard name
Human Ortholog
Description Subunit of tRNA (1-methyladenosine) methyltransferase; required, along with Gcd10p, for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 – 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Cytoplasm – – 0 0.06 0 0 0 0 0 0 0 0 0.05 0.1 0.15 0.09 0.11 0.06 0.25 – 0 0 0 0
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Endosome – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Mitochondria – – 0 0 0.05 0.24 0.36 0.17 0.27 0.32 0.39 0.32 0 0 0 0 0 0 0.48 – 0 0 0 0
Nucleus – – 0.97 0.92 0.95 0.88 0.85 0.86 0.85 0.81 0.81 0.79 0.94 0.88 0.82 0.86 0.84 0.85 0 – 0.91 0.94 0.87 0.8
Nuclear Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Nucleolus – – 0 0 0 0 0.06 0.08 0.1 0.09 0.15 0.13 0 0 0 0 0 0 0.11 – 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Vac/Vac Membrane – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 – 0 0 0.08 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud – – 0 0 2 1 6 5 3 6 0 6 0 0 1 0 0 0 0 – 1 1 1 3
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud Site – – 2 1 1 4 15 16 11 12 18 27 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Cytoplasm – – 5 18 10 14 17 30 11 9 6 6 16 42 78 15 22 8 0 – 2 0 3 4
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 5 3 0 – 0 0 0 0
Endosome – – 0 0 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 – 3 0 3 4
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 – 3 0 2 0
Mitochondria – – 16 9 20 134 322 160 132 224 227 250 1 2 1 2 8 3 1 – 2 0 1 2
Nucleus – – 385 277 397 495 755 804 411 561 473 618 276 381 417 143 168 121 0 – 367 181 370 329
Nuclear Periphery – – 4 3 3 8 9 29 6 12 9 24 3 10 2 1 5 0 0 – 0 0 1 2
Nucleolus – – 4 4 5 22 57 72 49 64 86 98 0 0 3 0 4 6 0 – 1 0 3 9
Peroxisomes – – 1 1 0 0 0 0 0 0 0 0 1 1 0 1 0 1 0 – 0 0 0 0
SpindlePole – – 1 0 0 0 0 0 0 0 0 0 0 6 3 0 1 3 0 – 1 0 0 2
Vac/Vac Membrane – – 0 2 5 2 13 12 1 12 3 5 0 1 6 9 7 7 0 – 15 4 31 47
Unique Cell Count 396 301 420 565 884 940 484 694 583 780 294 431 506 166 201 143 4 402 192 425 410
Labelled Cell Count 419 315 443 680 1194 1128 624 900 822 1035 297 446 513 172 220 152 4 402 192 425 410


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 6.6 6.6 5.9 4.8 4.6 4.8 4.7 4.6 4.3 4.4 8.0 7.4 7.3 10.2 9.7 11.0 7.7 8.3 8.7
Std Deviation (1e-4) – – 1.6 1.9 1.6 1.1 1.0 1.3 1.5 1.2 1.0 0.9 1.4 1.5 1.6 2.6 2.6 3.8 1.3 1.5 1.6
Intensity Change (Log2) – – – 0.01 -0.16 -0.45 -0.52 -0.44 -0.47 -0.51 -0.62 -0.59 0.28 0.17 0.15 0.64 0.56 0.74 0.23 0.33 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.5 1.2 1.3 0.8 2.0 1.1 0 0 0 3.2 5.3 7.3 4.5 5.4 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.7 0.5 0 0 0 0 0 0 0 -3.1 -3.5 -4.2 -1.7 0 -1.1
Nucleus -3.1 -1.9 -5.3 -6.3 -6.2 -6.2 -7.7 -7.5 -8.2 -2.2 -4.8 -7.0 -5.0 -6.0 -5.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1.5 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4151 4.7705 4.3147 4.0037 3.7646 4.2638 3.9186 4.6337 4.4779 3.884 3.5475 4.1039 3.0769 3.2733 2.8789 2.2792 2.4817 2.6446
Actin 0.006 0 0.0043 0.0007 0.036 0.0018 0.0273 0 0.014 0.0038 0.0135 0.0107 0.0303 0 0.004 0.0355 0.0052 0.0001
Bud 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0007 0 0.0009 0.0003 0.0014 0.0001 0.0004 0 0.0001 0.0001 0.0021 0
Bud Neck 0.0002 0 0.0002 0.0001 0.0006 0.0003 0.004 0 0.0004 0.0038 0.001 0.0007 0.001 0 0.0001 0.0002 0.0028 0.0004
Bud Periphery 0.0002 0.0003 0.0002 0.0004 0.0004 0.0001 0.0015 0 0.0018 0.0004 0.0029 0.0002 0.0007 0 0.0002 0.0002 0.0055 0
Bud Site 0.0001 0.0001 0.0007 0.0002 0.0005 0 0.008 0 0.0021 0.0045 0.0053 0.0002 0.0014 0 0.0001 0.0004 0.0053 0
Cell Periphery 0 0.0001 0.0002 0.0003 0.0001 0 0.0009 0 0.0003 0.0002 0.0004 0 0.0002 0 0 0 0.0016 0
Cytoplasm 0.0006 0 0.0001 0.0002 0.0013 0 0.0036 0.0001 0.0003 0.0005 0.0043 0.0003 0.0064 0 0.0001 0.0001 0.0028 0.0003
Cytoplasmic Foci 0.0086 0 0.0007 0.0004 0.0017 0.0002 0.0103 0.0002 0.0016 0.0246 0.007 0.0019 0.0062 0 0.0022 0.0181 0.0075 0.0004
Eisosomes 0.0001 0 0 0.0001 0.001 0 0.0005 0 0.0002 0.0001 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0013 0 0.0002 0.0012 0.0012 0.0002 0.0045 0.0004 0.0015 0.0029 0.0037 0.0009 0.0042 0 0.0001 0.0001 0.0018 0.0001
Endosome 0.0049 0 0.0015 0.0021 0.0047 0.0014 0.0154 0.0043 0.0036 0.0262 0.0409 0.0098 0.0126 0 0.0013 0.0046 0.0147 0.0005
Golgi 0.0042 0 0.0008 0.0008 0.0034 0.0011 0.0041 0.0006 0.0026 0.013 0.0123 0.0025 0.0041 0 0.0011 0.0039 0.0017 0
Lipid Particles 0.0071 0 0.002 0.0093 0.0027 0.0007 0.0127 0.0002 0.0071 0.0117 0.0244 0.0033 0.0052 0 0.0045 0.0477 0.0037 0.0001
Mitochondria 0.0015 0.0064 0.0006 0.0119 0.0026 0.0005 0.0162 0.0002 0.0069 0.0314 0.019 0.0112 0.0067 0.0001 0.0012 0.0012 0.0064 0.0002
None 0.0004 0 0.0002 0.0004 0.0176 0 0.0036 0 0.0003 0.0002 0.0038 0.0004 0.0031 0 0.0002 0.0002 0.0025 0.0002
Nuclear Periphery 0.0087 0.0008 0.0058 0.0075 0.0066 0.0016 0.0373 0.0021 0.0045 0.0127 0.0289 0.0036 0.0134 0.0007 0.0007 0.0002 0.0195 0.0052
Nucleolus 0.009 0.0068 0.0029 0.0147 0.068 0.0198 0.0144 0.005 0.0035 0.0047 0.0555 0.0147 0.0067 0.0064 0.0035 0.0025 0.0315 0.0185
Nucleus 0.9397 0.9846 0.9764 0.9432 0.8436 0.9714 0.8122 0.9861 0.9404 0.8362 0.7359 0.9346 0.885 0.9927 0.9762 0.8611 0.8577 0.9694
Peroxisomes 0.0047 0 0.0005 0.0002 0.0017 0.0001 0.0046 0 0.0067 0.0155 0.005 0.0013 0.0039 0 0.0026 0.0229 0.0037 0
Punctate Nuclear 0.0015 0.0003 0.0018 0.0004 0.005 0.0004 0.0065 0.0001 0.0003 0.0005 0.0081 0.0017 0.0056 0.0001 0.0013 0.0007 0.019 0.0041
Vacuole 0.0004 0.0002 0.0005 0.002 0.0004 0.0001 0.0062 0.0003 0.0007 0.003 0.0135 0.001 0.0015 0 0.0003 0.0001 0.0036 0.0002
Vacuole Periphery 0.0008 0.0002 0.0003 0.004 0.0009 0.0002 0.0055 0.0003 0.0007 0.0039 0.013 0.0008 0.0012 0 0.0003 0.0001 0.0012 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.072 28.2556 22.8729 19.4906 22.9742 13.402 19.9118 18.1136 18.7578 22.304
Translational Efficiency 1.9624 1.5677 1.5284 1.3564 1.4728 2.1141 1.8229 1.1445 1.0062 1.3609

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1884 978 477 1217 1618 1356 269 483 3502 2334 746 1700

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 856.87 865.16 1058.62 1064.12 797.96 887.92 1040.19 1102.12 829.65 878.38 1051.97 1074.92
Standard Deviation 114.73 158.12 133.47 183.41 107.64 120.50 132.61 160.48 115.31 137.98 133.45 178.02
Intensity Change Log 2 — 0.013891 0.305037 0.312513 — 0.154113 0.382459 0.465893 — 0.083209 0.342889 0.388510

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000202 0.000607 0.000775 0.000682 0.000189 0.000318 0.000600 0.000721 0.000196 0.000439 0.000712 0.000693
Bud Neck 0.003278 0.005742 0.013645 0.015071 0.002685 0.004285 0.004546 0.013246 0.003004 0.004895 0.010364 0.014552
Bud Site 0.000668 0.001757 0.001015 0.001323 0.000366 0.001168 0.000553 0.002016 0.000528 0.001415 0.000848 0.001520
Cell Periphery 0.000250 0.000289 0.000047 0.000077 0.000219 0.000143 0.000047 0.000083 0.000236 0.000204 0.000047 0.000078
Cytoplasm 0.000929 0.002508 0.000355 0.001164 0.001850 0.001554 0.000842 0.002850 0.001354 0.001954 0.000531 0.001643
Cytoplasmic Foci 0.000517 0.000631 0.000019 0.000463 0.000248 0.001154 0.000057 0.000029 0.000392 0.000935 0.000032 0.000339
Eisosomes 0.000023 0.000033 0.000042 0.000027 0.000020 0.000022 0.000035 0.000026 0.000022 0.000027 0.000040 0.000027
Endoplasmic Reticulum 0.007043 0.008362 0.004214 0.004756 0.006319 0.006841 0.005593 0.003758 0.006709 0.007478 0.004711 0.004472
Endosome 0.000590 0.001202 0.000099 0.000630 0.000247 0.000404 0.000121 0.000124 0.000432 0.000738 0.000107 0.000486
Golgi 0.000267 0.000403 0.000045 0.001234 0.000108 0.000228 0.000043 0.000057 0.000194 0.000301 0.000044 0.000899
Lipid Particles 0.000309 0.000546 0.000066 0.000052 0.000444 0.000161 0.000136 0.000041 0.000371 0.000322 0.000091 0.000049
Mitochondria 0.001861 0.000958 0.000982 0.002408 0.000550 0.001273 0.000578 0.001432 0.001256 0.001141 0.000836 0.002130
Mitotic Spindle 0.001279 0.002262 0.000137 0.002816 0.000574 0.001583 0.000120 0.006928 0.000954 0.001867 0.000131 0.003984
None 0.001323 0.003911 0.000825 0.001471 0.001992 0.002358 0.003460 0.003915 0.001632 0.003009 0.001776 0.002166
Nuclear Periphery 0.000546 0.000231 0.000191 0.000581 0.000326 0.000329 0.000200 0.000480 0.000444 0.000288 0.000195 0.000553
Nuclear Periphery Foci 0.000838 0.000413 0.000031 0.000310 0.000287 0.000120 0.000077 0.000067 0.000584 0.000243 0.000047 0.000241
Nucleolus 0.004759 0.007909 0.004590 0.004318 0.004651 0.005249 0.006610 0.004956 0.004709 0.006364 0.005318 0.004499
Nucleus 0.973148* 0.959880* 0.971413* 0.959250* 0.977618* 0.971481* 0.974963* 0.957939* 0.975214* 0.966620* 0.972693* 0.958877*
Peroxisomes 0.000745 0.000471 0.000278 0.000155 0.000495 0.000269 0.000220 0.000141 0.000629 0.000354 0.000257 0.000151
Vacuole 0.001050 0.001481 0.001145 0.002382 0.000741 0.000778 0.001134 0.001088 0.000907 0.001073 0.001141 0.002014
Vacuole Periphery 0.000373 0.000403 0.000084 0.000832 0.000072 0.000280 0.000067 0.000105 0.000234 0.000331 0.000078 0.000625

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.58 -12.66 -10.90 -1.36 1.57 -2.51 -8.17 -9.43 -7.71 -4.18 -5.21 -14.85 -14.21 -6.47 -1.01
Bud Neck -2.53 -7.89 -12.90 -8.61 -1.90 -2.49 -2.25 -6.71 -5.90 -5.45 -3.48 -8.11 -14.43 -11.65 -4.86
Bud Site -2.47 -2.10 -2.79 0.39 -1.08 -2.12 -2.11 -3.06 -1.53 -2.60 -2.97 -3.03 -4.57 -1.04 -2.74
Cell Periphery -0.59 4.47 3.83 4.12 -2.79 1.03 2.78 2.21 1.41 -1.35 0.64 5.01 4.19 3.78 -3.04
Cytoplasm -1.75 2.01 -0.70 1.49 -3.68 0.17 0.64 -0.79 -0.87 -1.11 -1.19 1.84 -0.73 0.45 -2.23
Cytoplasmic Foci -0.30 3.02 0.76 0.89 -1.84 -1.28 1.61 1.77 1.66 1.26 -1.23 3.44 0.89 1.57 -1.77
Eisosomes -3.35 -8.03 -2.36 1.95 6.98 -0.57 -5.58 -4.59 -4.54 1.76 -2.67 -9.98 -4.56 -1.34 6.91
Endoplasmic Reticulum -1.88 5.18 4.81 5.65 -0.29 -0.95 0.94 3.29 4.57 2.10 -1.78 4.64 5.60 7.04 1.00
Endosome -0.95 2.81 0.19 0.97 -1.37 -1.25 1.62 0.63 1.96 -2.30 -1.06 3.20 -0.02 0.85 -1.46
Golgi -0.61 2.18 -0.90 -0.69 -1.20 -0.57 1.03 0.27 0.71 -3.84 -0.70 2.39 -0.95 -0.74 -1.22
Lipid Particles -0.81 2.27 2.43 1.61 0.98 0.84 0.91 0.99 1.14 0.88 0.18 1.62 1.77 1.90 1.59
Mitochondria 1.33 1.15 -0.97 -4.36 -4.30 -1.67 -0.26 -5.32 -1.52 -7.29 0.26 0.92 -2.59 -3.67 -5.87
Mitotic Spindle -0.68 1.85 -1.84 -0.86 -2.78 -1.13 0.62 -2.55 -2.22 -2.65 -1.14 1.90 -3.23 -2.34 -3.82
None -4.09 1.59 -1.05 3.26 -2.29 -0.50 -1.26 -0.93 -0.68 -0.01 -2.97 -0.35 -1.05 1.39 -0.67
Nuclear Periphery 1.76 1.64 -1.01 -7.58 -6.92 -0.12 2.55 -5.17 -5.11 -6.40 1.54 2.08 -3.08 -8.35 -9.40
Nuclear Periphery Foci 1.23 2.41 1.19 0.03 -1.71 1.29 1.61 0.19 -0.94 -1.22 1.77 2.81 0.99 -0.70 -1.95
Nucleolus -2.44 -0.17 -1.37 1.18 -1.26 -0.44 -1.23 -0.78 -0.56 0.17 -2.10 -1.00 -1.57 0.16 -0.75
Nucleus 3.05 1.53 5.20 1.54 4.02 1.94 1.07 5.21 4.09 4.50 3.27 2.12 7.64 4.58 6.01
Peroxisomes 0.56 1.07 1.35 2.12 3.61 0.89 1.02 1.14 1.23 1.29 1.08 1.45 1.77 2.49 3.76
Vacuole -1.01 -0.99 -2.30 -1.31 -0.83 -0.66 -1.65 -2.95 -2.75 -2.37 -1.04 -1.45 -3.58 -2.92 -1.77
Vacuole Periphery -0.26 2.05 -0.78 -0.67 -1.49 -1.95 0.02 -2.83 1.38 -2.98 -0.96 1.99 -1.03 -0.67 -1.55
Standard name
Human Ortholog
Description Subunit of tRNA (1-methyladenosine) methyltransferase; required, along with Gcd10p, for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Gcd14

Gcd14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcd14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available