Standard name
Human Ortholog
Description Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.06 0.06 0.07 0.13 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 1.0 0.96 0.92 0.79 0.76 0.74 0.77 0.71 0.68 1.0 1.0 0.99 0.98 0.98 0.98 0.98 0.98 0.94 0.95 0.97 0.95
Endoplasmic Reticulum 0 0 0 0 0 0.06 0.18 0.19 0.2 0.13 0.16 0.2 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 4 7 7 9 22 14 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 7 0 1 2 1 3 0 0 0 0 0 1 1 0 1 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 4 4 6 8 14 23 21 23 51 46 0 10 5 5 5 7 0 0 1 0 0 0
Cytoplasm 259 326 402 272 290 374 401 277 261 261 270 245 189 410 422 429 320 259 260 338 386 199 233 268
Endoplasmic Reticulum 0 1 1 0 10 24 91 70 72 43 59 72 0 2 3 25 19 14 2 0 12 0 1 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 1 1 2 4 1 4 3 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3 5 1 2 8 6 6 0 0 0 0 0 0 0 0 1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 0 0 0 1 2 3 0 0 1 2 7 1 0 0 1 4 1 0
Unique Cell Count 259 328 403 272 301 406 510 365 353 338 379 359 189 412 425 436 326 263 267 346 410 210 242 283
Labelled Cell Count 260 330 407 276 306 413 523 380 370 348 416 393 189 422 431 462 353 282 267 346 410 210 242 283


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 121.8 173.8 128.1 108.5 89.5 79.0 74.1 74.2 70.2 67.1 61.0 60.8 144.0 139.6 127.3 218.4 182.3 211.1 165.5 172.2 177.9
Std Deviation (1e-4) 20.9 32.2 26.4 27.6 21.6 20.0 17.9 16.2 18.3 16.3 15.2 14.9 32.9 27.3 26.3 76.0 63.5 75.4 46.4 41.6 46.0
Intensity Change (Log2) – – – -0.24 -0.52 -0.7 -0.79 -0.79 -0.87 -0.93 -1.07 -1.08 0.17 0.12 -0.01 0.77 0.51 0.72 0.37 0.43 0.47

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000100200WT3HU80HU120HU1600100200WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30100200WT1AF100AF140AF1800100200
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 4.9 4.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.2 1.9 4.0 3.8 4.2 6.8 6.6 0 1.6 0 0 0 0
Cytoplasm 0.8 -3.5 -5.5 -9.7 -10.3 -10.8 -10.0 -11.5 -12.1 0.7 -0.6 -0.9 -2.0 -2.2 -1.9
Endoplasmic Reticulum 0 0 4.7 8.8 9.0 9.4 7.2 8.0 9.3 0 0 0 4.6 4.6 4.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 123.1085 158.5018 152.5323 150.1439 149.0027 156.8849 128.2078 190.4876 175.7217 150.3948 162.8587 162.6441 149.5423 171.9735 164.3583 157.2301 146.2816 152.5626
Actin 0.0124 0.0001 0.0013 0.0005 0.0005 0.0004 0.01 0.0002 0.0002 0.0344 0.0151 0.0019 0.0036 0.0002 0.003 0.0084 0.0002 0.0006
Bud 0.0015 0.0003 0.0005 0.0011 0.0008 0.0006 0.0019 0.0007 0.0009 0.0019 0.002 0.0024 0.0009 0.0004 0.0007 0.0011 0.001 0.0009
Bud Neck 0.0024 0.0004 0.001 0.0012 0.0036 0.0025 0.0041 0.0006 0.0012 0.0019 0.0019 0.0063 0.0009 0.0005 0.0008 0.0016 0.0009 0.0024
Bud Periphery 0.0017 0.0003 0.0003 0.0015 0.0007 0.0006 0.0031 0.0005 0.0008 0.0026 0.0013 0.0026 0.0021 0.0005 0.0008 0.001 0.0014 0.0008
Bud Site 0.0027 0.0003 0.0004 0.0002 0.0002 0.0001 0.0058 0.0008 0.0005 0.0006 0.0012 0.0007 0.0004 0.0003 0.0003 0.0009 0.0008 0.0002
Cell Periphery 0.0461 0.0311 0.0299 0.0254 0.0057 0.0087 0.0873 0.0714 0.0686 0.0469 0.0543 0.0138 0.0404 0.0296 0.0266 0.0361 0.0056 0.0083
Cytoplasm 0.6137 0.8826 0.8263 0.721 0.8063 0.854 0.5452 0.7765 0.6846 0.6456 0.5367 0.8093 0.6951 0.8851 0.8261 0.6257 0.7573 0.8298
Cytoplasmic Foci 0.0104 0.0005 0.0039 0.0018 0.002 0.002 0.0114 0.0004 0.005 0.0011 0.0027 0.005 0.0076 0.0005 0.0026 0.0055 0.0048 0.0032
Eisosomes 0.0008 0 0.0001 0 0 0 0.0026 0 0.0001 0.0004 0.0006 0.0001 0.0003 0.0002 0.0002 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0456 0.0083 0.0174 0.0126 0.0232 0.0303 0.0265 0.0052 0.0136 0.0145 0.0516 0.0297 0.0585 0.0136 0.0262 0.0414 0.0115 0.0643
Endosome 0.0091 0.001 0.0026 0.0107 0.0069 0.0079 0.0055 0.0006 0.0012 0.0037 0.0073 0.004 0.0029 0.0008 0.0021 0.0093 0.0112 0.0054
Golgi 0.0037 0.0001 0.0004 0.0009 0.0007 0.0012 0.0016 0.0001 0.0001 0.0018 0.0025 0.0007 0.0006 0.0001 0.0004 0.0023 0.0009 0.0009
Lipid Particles 0.0102 0.0019 0.0028 0.0047 0.0016 0.0021 0.013 0.0022 0.0053 0.0027 0.0156 0.0032 0.005 0.0021 0.0026 0.0065 0.0026 0.0021
Mitochondria 0.0103 0.0001 0.0009 0.0006 0.0015 0.001 0.0083 0.0002 0.0038 0.0015 0.0111 0.0011 0.0061 0.0004 0.0021 0.0018 0.004 0.0021
None 0.0054 0.0002 0.002 0.0003 0.0002 0.0004 0.0212 0.0002 0.0011 0.0007 0.0025 0.0014 0.0035 0.0002 0.0036 0.0049 0.0082 0.0008
Nuclear Periphery 0.022 0.0031 0.0075 0.0074 0.0097 0.0069 0.0296 0.0049 0.0096 0.0105 0.0166 0.009 0.0169 0.0043 0.0076 0.0105 0.0059 0.0099
Nucleolus 0.0124 0.0002 0.0023 0.0004 0.0001 0.0006 0.0283 0.0007 0.0096 0.0011 0.0038 0.0036 0.0093 0.0003 0.002 0.0158 0.0119 0.0067
Nucleus 0.0119 0.0014 0.0037 0.0034 0.0023 0.0031 0.0199 0.0038 0.0077 0.007 0.0133 0.0183 0.0095 0.002 0.0048 0.0083 0.0241 0.0047
Peroxisomes 0.0056 0 0.0002 0 0.0001 0.0001 0.0016 0 0.0003 0.0002 0.0037 0.0002 0.0006 0 0.0002 0.0009 0.0005 0.0001
Punctate Nuclear 0.0101 0 0.0018 0.0001 0.0001 0.0001 0.0023 0 0.0002 0.0004 0.0078 0.0005 0.0011 0 0.0003 0.0049 0.0005 0.0001
Vacuole 0.1394 0.0654 0.0878 0.1787 0.1136 0.0586 0.153 0.1272 0.176 0.2015 0.2257 0.0712 0.1213 0.0567 0.0824 0.1876 0.1194 0.0492
Vacuole Periphery 0.0225 0.0025 0.0066 0.0276 0.0201 0.0188 0.0177 0.0038 0.0096 0.0189 0.0226 0.015 0.0136 0.0022 0.0047 0.025 0.0271 0.0074

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1812.0724 1780.957 1784.8585 1547.7143 1408.334 1585.4979 1587.4912 1360.9846 1312.8922 1368.7419
Translational Efficiency 0.8043 0.8589 0.7891 0.8695 0.9189 0.8969 0.8699 0.9218 0.8477 0.8325

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1098 250 2381 1120 1375 1629 1336 935 2473 1879 3717 2055

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 7542.07 10191.92 8652.92 14966.10 8163.05 8635.29 11105.29 15778.05 7887.34 8842.40 9534.37 15335.53
Standard Deviation 1626.93 1859.68 2163.31 3703.93 1627.11 2386.32 2652.09 3670.81 1656.03 2382.54 2628.80 3710.99
Intensity Change Log 2 — 0.434393 0.198227 0.988666 — 0.081136 0.444067 0.950739 — 0.261584 0.331217 0.969077

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000052 0.000191 0.000074 0.000260 0.000069 0.000062 0.000224 0.000243 0.000061 0.000079 0.000128 0.000252
Bud Neck 0.000267 0.001831 0.001037 0.001227 0.000610 0.000369 0.003435 0.001644 0.000458 0.000563 0.001899 0.001416
Bud Site 0.001568 0.005235 0.005212 0.012379 0.002227 0.001851 0.005441 0.008756 0.001934 0.002301 0.005294 0.010731
Cell Periphery 0.026743 0.047399 0.007648 0.051185 0.027514 0.012724 0.016276 0.051989 0.027171 0.017338 0.010749 0.051551
Cytoplasm 0.705347 0.477815 0.706952 0.614057 0.638517 0.804620 0.609189 0.471531 0.668189 0.761139 0.671813 0.549209
Cytoplasmic Foci 0.007660 0.024009 0.004852 0.003811 0.011944 0.007567 0.010907 0.004457 0.010042 0.009754 0.007028 0.004105
Eisosomes 0.000002 0.000007 0.000001 0.000005 0.000003 0.000001 0.000005 0.000005 0.000002 0.000002 0.000003 0.000005
Endoplasmic Reticulum 0.001283 0.000540 0.000607 0.000470 0.001367 0.000570 0.000272 0.000277 0.001330 0.000566 0.000486 0.000382
Endosome 0.000562 0.000858 0.000794 0.000524 0.000715 0.000361 0.000695 0.000761 0.000647 0.000427 0.000759 0.000632
Golgi 0.053892 0.091926 0.010216 0.010916 0.071509 0.035777 0.022147 0.015706 0.063687 0.043248 0.014505 0.013096
Lipid Particles 0.016635 0.034132 0.008783 0.033126 0.021562 0.010378 0.020422 0.055051 0.019374 0.013538 0.012966 0.043101
Mitochondria 0.000524 0.000338 0.000259 0.000260 0.000202 0.000152 0.000246 0.000255 0.000345 0.000177 0.000254 0.000258
Mitotic Spindle 0.000733 0.002477 0.000544 0.000469 0.001412 0.000826 0.001605 0.000507 0.001110 0.001046 0.000925 0.000486
None 0.002147 0.001859 0.001440 0.002242 0.002470 0.006340 0.001988 0.001187 0.002326 0.005744 0.001637 0.001762
Nuclear Periphery 0.000729 0.001899 0.000266 0.000398 0.001462 0.000520 0.001100 0.000529 0.001137 0.000703 0.000566 0.000458
Nuclear Periphery Foci 0.011534 0.019817 0.004176 0.004770 0.018613 0.009808 0.011608 0.007334 0.015470 0.011140 0.006848 0.005937
Nucleolus 0.000063 0.001228 0.000146 0.000186 0.000071 0.000092 0.000141 0.000148 0.000067 0.000243 0.000145 0.000169
Nucleus 0.008996 0.021216 0.013693 0.010917 0.010243 0.011980 0.036388 0.010902 0.009689 0.013209 0.021850 0.010910
Peroxisomes 0.000024 0.000184 0.000073 0.000154 0.000017 0.000020 0.000077 0.000104 0.000020 0.000042 0.000075 0.000131
Vacuole 0.084455 0.178436 0.201681 0.224532 0.098714 0.059372 0.227535 0.344959 0.092383 0.075213 0.210973 0.279325
Vacuole Periphery 0.076787 0.088603 0.031545 0.028110 0.090760 0.036612 0.030299 0.023656 0.084556 0.043529 0.031097 0.026084

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.45 -3.72 -7.32 -1.57 -5.86 0.81 -4.24 -4.46 -4.70 0.14 -1.66 -4.98 -8.06 -7.12 -3.78
Bud Neck -4.32 -11.08 -10.65 1.24 -0.09 1.61 -9.78 -6.14 -7.61 6.19 -1.56 -12.55 -10.21 -7.50 4.74
Bud Site -3.34 -9.65 -9.06 -4.48 -5.31 0.74 -5.28 -6.02 -6.52 -2.28 -1.20 -9.49 -10.64 -9.82 -5.67
Cell Periphery -4.71 11.21 -6.57 -0.05 -14.90 8.66 6.65 -6.85 -12.93 -11.65 6.83 13.93 -9.43 -14.57 -19.41
Cytoplasm 9.31 1.73 6.63 -5.43 6.10 -13.99 4.37 11.22 24.92 7.45 -9.13 2.33 11.57 20.06 10.59
Cytoplasmic Foci -5.89 3.87 4.22 6.88 1.19 4.25 -1.23 5.53 2.44 6.47 -0.22 2.77 7.26 6.84 5.48
Eisosomes -3.68 -0.20 -6.66 0.77 -6.64 5.96 -4.66 -4.54 -7.60 0.84 1.32 -3.42 -7.65 -8.04 -4.51
Endoplasmic Reticulum 1.99 1.91 2.31 0.67 1.28 3.76 7.42 7.50 2.07 0.08 3.41 4.57 5.32 1.57 1.56
Endosome -2.44 -2.34 0.27 2.41 2.31 5.74 0.18 0.11 -2.43 0.01 3.84 -1.87 0.45 -1.90 1.78
Golgi -4.84 16.95 16.39 11.64 -0.52 11.35 18.48 18.99 9.57 3.27 8.11 26.22 25.56 15.85 1.49
Lipid Particles -5.30 4.81 -5.70 0.75 -9.92 7.08 0.67 -9.65 -14.03 -10.18 4.43 5.44 -10.57 -13.89 -14.81
Mitochondria 0.49 0.72 0.68 0.30 -0.14 1.19 -1.72 -0.66 -0.98 0.24 1.06 0.29 0.30 -1.39 0.08
Mitotic Spindle -4.14 0.61 1.51 4.77 0.91 0.96 -0.70 3.53 1.84 3.43 -0.50 0.49 3.89 2.89 3.34
None 0.79 1.43 -0.07 -0.58 -1.16 -2.89 0.76 1.86 3.99 1.84 -3.07 1.61 1.20 3.58 -0.31
Nuclear Periphery -5.44 8.46 6.26 6.97 -2.93 9.70 1.97 9.84 -0.03 9.48 5.91 8.12 11.75 5.24 5.11
Nuclear Periphery Foci -3.65 9.87 9.51 6.86 -0.71 8.00 6.90 11.59 3.53 6.03 4.97 13.33 15.31 7.86 3.02
Nucleolus -1.03 -6.77 -6.37 0.91 -0.85 -0.96 -7.28 -4.22 -2.16 0.36 -1.18 -8.98 -7.49 0.43 -0.43
Nucleus -2.79 -7.07 -4.22 1.60 2.27 -1.32 -13.65 -2.52 -1.79 10.94 -3.29 -14.02 -4.42 -1.11 9.12
Peroxisomes -1.01 -5.82 -6.69 0.20 -4.27 -0.30 -1.10 -5.72 -5.10 -0.63 -0.97 -2.88 -8.77 -3.42 -2.88
Vacuole -6.82 -19.31 -16.63 -3.05 -1.24 7.31 -16.62 -24.04 -28.87 -8.82 3.46 -25.03 -27.97 -29.52 -7.60
Vacuole Periphery -1.00 11.23 11.37 7.94 1.45 12.45 15.47 16.68 5.99 2.32 12.02 18.99 19.76 8.08 2.74
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Tif2

Tif2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tif2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available