Standard name | |
---|---|
Human Ortholog | |
Description | Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 7 | 7 | 9 | 22 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 7 | 0 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 4 | 4 | 6 | 8 | 14 | 23 | 21 | 23 | 51 | 46 | 0 | 10 | 5 | 5 | 5 | 7 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 259 | 326 | 402 | 272 | 290 | 374 | 401 | 277 | 261 | 261 | 270 | 245 | 189 | 410 | 422 | 429 | 320 | 259 | 260 | 338 | 386 | 199 | 233 | 268 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 10 | 24 | 91 | 70 | 72 | 43 | 59 | 72 | 0 | 2 | 3 | 25 | 19 | 14 | 2 | 0 | 12 | 0 | 1 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 4 | 1 | 4 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 1 | 2 | 8 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 0 | 1 | 2 | 7 | 1 | 0 | 0 | 1 | 4 | 1 | 0 |
Unique Cell Count | 259 | 328 | 403 | 272 | 301 | 406 | 510 | 365 | 353 | 338 | 379 | 359 | 189 | 412 | 425 | 436 | 326 | 263 | 267 | 346 | 410 | 210 | 242 | 283 |
Labelled Cell Count | 260 | 330 | 407 | 276 | 306 | 413 | 523 | 380 | 370 | 348 | 416 | 393 | 189 | 422 | 431 | 462 | 353 | 282 | 267 | 346 | 410 | 210 | 242 | 283 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 121.8 | 173.8 | 128.1 | 108.5 | 89.5 | 79.0 | 74.1 | 74.2 | 70.2 | 67.1 | 61.0 | 60.8 | 144.0 | 139.6 | 127.3 | 218.4 | 182.3 | 211.1 | 165.5 | 172.2 | 177.9 |
Std Deviation (1e-4) | 20.9 | 32.2 | 26.4 | 27.6 | 21.6 | 20.0 | 17.9 | 16.2 | 18.3 | 16.3 | 15.2 | 14.9 | 32.9 | 27.3 | 26.3 | 76.0 | 63.5 | 75.4 | 46.4 | 41.6 | 46.0 |
Intensity Change (Log2) | – | – | – | -0.24 | -0.52 | -0.7 | -0.79 | -0.79 | -0.87 | -0.93 | -1.07 | -1.08 | 0.17 | 0.12 | -0.01 | 0.77 | 0.51 | 0.72 | 0.37 | 0.43 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.9 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.2 | 1.9 | 4.0 | 3.8 | 4.2 | 6.8 | 6.6 | 0 | 1.6 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8 | -3.5 | -5.5 | -9.7 | -10.3 | -10.8 | -10.0 | -11.5 | -12.1 | 0.7 | -0.6 | -0.9 | -2.0 | -2.2 | -1.9 |
Endoplasmic Reticulum | 0 | 0 | 4.7 | 8.8 | 9.0 | 9.4 | 7.2 | 8.0 | 9.3 | 0 | 0 | 0 | 4.6 | 4.6 | 4.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 123.1085 | 158.5018 | 152.5323 | 150.1439 | 149.0027 | 156.8849 | 128.2078 | 190.4876 | 175.7217 | 150.3948 | 162.8587 | 162.6441 | 149.5423 | 171.9735 | 164.3583 | 157.2301 | 146.2816 | 152.5626 |
Actin | 0.0124 | 0.0001 | 0.0013 | 0.0005 | 0.0005 | 0.0004 | 0.01 | 0.0002 | 0.0002 | 0.0344 | 0.0151 | 0.0019 | 0.0036 | 0.0002 | 0.003 | 0.0084 | 0.0002 | 0.0006 |
Bud | 0.0015 | 0.0003 | 0.0005 | 0.0011 | 0.0008 | 0.0006 | 0.0019 | 0.0007 | 0.0009 | 0.0019 | 0.002 | 0.0024 | 0.0009 | 0.0004 | 0.0007 | 0.0011 | 0.001 | 0.0009 |
Bud Neck | 0.0024 | 0.0004 | 0.001 | 0.0012 | 0.0036 | 0.0025 | 0.0041 | 0.0006 | 0.0012 | 0.0019 | 0.0019 | 0.0063 | 0.0009 | 0.0005 | 0.0008 | 0.0016 | 0.0009 | 0.0024 |
Bud Periphery | 0.0017 | 0.0003 | 0.0003 | 0.0015 | 0.0007 | 0.0006 | 0.0031 | 0.0005 | 0.0008 | 0.0026 | 0.0013 | 0.0026 | 0.0021 | 0.0005 | 0.0008 | 0.001 | 0.0014 | 0.0008 |
Bud Site | 0.0027 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0058 | 0.0008 | 0.0005 | 0.0006 | 0.0012 | 0.0007 | 0.0004 | 0.0003 | 0.0003 | 0.0009 | 0.0008 | 0.0002 |
Cell Periphery | 0.0461 | 0.0311 | 0.0299 | 0.0254 | 0.0057 | 0.0087 | 0.0873 | 0.0714 | 0.0686 | 0.0469 | 0.0543 | 0.0138 | 0.0404 | 0.0296 | 0.0266 | 0.0361 | 0.0056 | 0.0083 |
Cytoplasm | 0.6137 | 0.8826 | 0.8263 | 0.721 | 0.8063 | 0.854 | 0.5452 | 0.7765 | 0.6846 | 0.6456 | 0.5367 | 0.8093 | 0.6951 | 0.8851 | 0.8261 | 0.6257 | 0.7573 | 0.8298 |
Cytoplasmic Foci | 0.0104 | 0.0005 | 0.0039 | 0.0018 | 0.002 | 0.002 | 0.0114 | 0.0004 | 0.005 | 0.0011 | 0.0027 | 0.005 | 0.0076 | 0.0005 | 0.0026 | 0.0055 | 0.0048 | 0.0032 |
Eisosomes | 0.0008 | 0 | 0.0001 | 0 | 0 | 0 | 0.0026 | 0 | 0.0001 | 0.0004 | 0.0006 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0456 | 0.0083 | 0.0174 | 0.0126 | 0.0232 | 0.0303 | 0.0265 | 0.0052 | 0.0136 | 0.0145 | 0.0516 | 0.0297 | 0.0585 | 0.0136 | 0.0262 | 0.0414 | 0.0115 | 0.0643 |
Endosome | 0.0091 | 0.001 | 0.0026 | 0.0107 | 0.0069 | 0.0079 | 0.0055 | 0.0006 | 0.0012 | 0.0037 | 0.0073 | 0.004 | 0.0029 | 0.0008 | 0.0021 | 0.0093 | 0.0112 | 0.0054 |
Golgi | 0.0037 | 0.0001 | 0.0004 | 0.0009 | 0.0007 | 0.0012 | 0.0016 | 0.0001 | 0.0001 | 0.0018 | 0.0025 | 0.0007 | 0.0006 | 0.0001 | 0.0004 | 0.0023 | 0.0009 | 0.0009 |
Lipid Particles | 0.0102 | 0.0019 | 0.0028 | 0.0047 | 0.0016 | 0.0021 | 0.013 | 0.0022 | 0.0053 | 0.0027 | 0.0156 | 0.0032 | 0.005 | 0.0021 | 0.0026 | 0.0065 | 0.0026 | 0.0021 |
Mitochondria | 0.0103 | 0.0001 | 0.0009 | 0.0006 | 0.0015 | 0.001 | 0.0083 | 0.0002 | 0.0038 | 0.0015 | 0.0111 | 0.0011 | 0.0061 | 0.0004 | 0.0021 | 0.0018 | 0.004 | 0.0021 |
None | 0.0054 | 0.0002 | 0.002 | 0.0003 | 0.0002 | 0.0004 | 0.0212 | 0.0002 | 0.0011 | 0.0007 | 0.0025 | 0.0014 | 0.0035 | 0.0002 | 0.0036 | 0.0049 | 0.0082 | 0.0008 |
Nuclear Periphery | 0.022 | 0.0031 | 0.0075 | 0.0074 | 0.0097 | 0.0069 | 0.0296 | 0.0049 | 0.0096 | 0.0105 | 0.0166 | 0.009 | 0.0169 | 0.0043 | 0.0076 | 0.0105 | 0.0059 | 0.0099 |
Nucleolus | 0.0124 | 0.0002 | 0.0023 | 0.0004 | 0.0001 | 0.0006 | 0.0283 | 0.0007 | 0.0096 | 0.0011 | 0.0038 | 0.0036 | 0.0093 | 0.0003 | 0.002 | 0.0158 | 0.0119 | 0.0067 |
Nucleus | 0.0119 | 0.0014 | 0.0037 | 0.0034 | 0.0023 | 0.0031 | 0.0199 | 0.0038 | 0.0077 | 0.007 | 0.0133 | 0.0183 | 0.0095 | 0.002 | 0.0048 | 0.0083 | 0.0241 | 0.0047 |
Peroxisomes | 0.0056 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0016 | 0 | 0.0003 | 0.0002 | 0.0037 | 0.0002 | 0.0006 | 0 | 0.0002 | 0.0009 | 0.0005 | 0.0001 |
Punctate Nuclear | 0.0101 | 0 | 0.0018 | 0.0001 | 0.0001 | 0.0001 | 0.0023 | 0 | 0.0002 | 0.0004 | 0.0078 | 0.0005 | 0.0011 | 0 | 0.0003 | 0.0049 | 0.0005 | 0.0001 |
Vacuole | 0.1394 | 0.0654 | 0.0878 | 0.1787 | 0.1136 | 0.0586 | 0.153 | 0.1272 | 0.176 | 0.2015 | 0.2257 | 0.0712 | 0.1213 | 0.0567 | 0.0824 | 0.1876 | 0.1194 | 0.0492 |
Vacuole Periphery | 0.0225 | 0.0025 | 0.0066 | 0.0276 | 0.0201 | 0.0188 | 0.0177 | 0.0038 | 0.0096 | 0.0189 | 0.0226 | 0.015 | 0.0136 | 0.0022 | 0.0047 | 0.025 | 0.0271 | 0.0074 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1812.0724 | 1780.957 | 1784.8585 | 1547.7143 | 1408.334 | 1585.4979 | 1587.4912 | 1360.9846 | 1312.8922 | 1368.7419 |
Translational Efficiency | 0.8043 | 0.8589 | 0.7891 | 0.8695 | 0.9189 | 0.8969 | 0.8699 | 0.9218 | 0.8477 | 0.8325 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1098 | 250 | 2381 | 1120 | 1375 | 1629 | 1336 | 935 | 2473 | 1879 | 3717 | 2055 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 7542.07 | 10191.92 | 8652.92 | 14966.10 | 8163.05 | 8635.29 | 11105.29 | 15778.05 | 7887.34 | 8842.40 | 9534.37 | 15335.53 |
Standard Deviation | 1626.93 | 1859.68 | 2163.31 | 3703.93 | 1627.11 | 2386.32 | 2652.09 | 3670.81 | 1656.03 | 2382.54 | 2628.80 | 3710.99 |
Intensity Change Log 2 | — | 0.434393 | 0.198227 | 0.988666 | — | 0.081136 | 0.444067 | 0.950739 | — | 0.261584 | 0.331217 | 0.969077 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000052 | 0.000191 | 0.000074 | 0.000260 | 0.000069 | 0.000062 | 0.000224 | 0.000243 | 0.000061 | 0.000079 | 0.000128 | 0.000252 |
Bud Neck | 0.000267 | 0.001831 | 0.001037 | 0.001227 | 0.000610 | 0.000369 | 0.003435 | 0.001644 | 0.000458 | 0.000563 | 0.001899 | 0.001416 |
Bud Site | 0.001568 | 0.005235 | 0.005212 | 0.012379 | 0.002227 | 0.001851 | 0.005441 | 0.008756 | 0.001934 | 0.002301 | 0.005294 | 0.010731 |
Cell Periphery | 0.026743 | 0.047399 | 0.007648 | 0.051185 | 0.027514 | 0.012724 | 0.016276 | 0.051989 | 0.027171 | 0.017338 | 0.010749 | 0.051551 |
Cytoplasm | 0.705347* | 0.477815* | 0.706952* | 0.614057* | 0.638517* | 0.804620* | 0.609189* | 0.471531* | 0.668189* | 0.761139* | 0.671813* | 0.549209* |
Cytoplasmic Foci | 0.007660 | 0.024009 | 0.004852 | 0.003811 | 0.011944 | 0.007567 | 0.010907 | 0.004457 | 0.010042 | 0.009754 | 0.007028 | 0.004105 |
Eisosomes | 0.000002 | 0.000007 | 0.000001 | 0.000005 | 0.000003 | 0.000001 | 0.000005 | 0.000005 | 0.000002 | 0.000002 | 0.000003 | 0.000005 |
Endoplasmic Reticulum | 0.001283 | 0.000540 | 0.000607 | 0.000470 | 0.001367 | 0.000570 | 0.000272 | 0.000277 | 0.001330 | 0.000566 | 0.000486 | 0.000382 |
Endosome | 0.000562 | 0.000858 | 0.000794 | 0.000524 | 0.000715 | 0.000361 | 0.000695 | 0.000761 | 0.000647 | 0.000427 | 0.000759 | 0.000632 |
Golgi | 0.053892 | 0.091926 | 0.010216 | 0.010916 | 0.071509 | 0.035777 | 0.022147 | 0.015706 | 0.063687 | 0.043248 | 0.014505 | 0.013096 |
Lipid Particles | 0.016635 | 0.034132 | 0.008783 | 0.033126 | 0.021562 | 0.010378 | 0.020422 | 0.055051 | 0.019374 | 0.013538 | 0.012966 | 0.043101 |
Mitochondria | 0.000524 | 0.000338 | 0.000259 | 0.000260 | 0.000202 | 0.000152 | 0.000246 | 0.000255 | 0.000345 | 0.000177 | 0.000254 | 0.000258 |
Mitotic Spindle | 0.000733 | 0.002477 | 0.000544 | 0.000469 | 0.001412 | 0.000826 | 0.001605 | 0.000507 | 0.001110 | 0.001046 | 0.000925 | 0.000486 |
None | 0.002147 | 0.001859 | 0.001440 | 0.002242 | 0.002470 | 0.006340 | 0.001988 | 0.001187 | 0.002326 | 0.005744 | 0.001637 | 0.001762 |
Nuclear Periphery | 0.000729 | 0.001899 | 0.000266 | 0.000398 | 0.001462 | 0.000520 | 0.001100 | 0.000529 | 0.001137 | 0.000703 | 0.000566 | 0.000458 |
Nuclear Periphery Foci | 0.011534 | 0.019817 | 0.004176 | 0.004770 | 0.018613 | 0.009808 | 0.011608 | 0.007334 | 0.015470 | 0.011140 | 0.006848 | 0.005937 |
Nucleolus | 0.000063 | 0.001228 | 0.000146 | 0.000186 | 0.000071 | 0.000092 | 0.000141 | 0.000148 | 0.000067 | 0.000243 | 0.000145 | 0.000169 |
Nucleus | 0.008996 | 0.021216 | 0.013693 | 0.010917 | 0.010243 | 0.011980 | 0.036388 | 0.010902 | 0.009689 | 0.013209 | 0.021850 | 0.010910 |
Peroxisomes | 0.000024 | 0.000184 | 0.000073 | 0.000154 | 0.000017 | 0.000020 | 0.000077 | 0.000104 | 0.000020 | 0.000042 | 0.000075 | 0.000131 |
Vacuole | 0.084455 | 0.178436 | 0.201681* | 0.224532* | 0.098714 | 0.059372 | 0.227535* | 0.344959* | 0.092383 | 0.075213 | 0.210973* | 0.279325* |
Vacuole Periphery | 0.076787 | 0.088603 | 0.031545 | 0.028110 | 0.090760 | 0.036612 | 0.030299 | 0.023656 | 0.084556 | 0.043529 | 0.031097 | 0.026084 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.45 | -3.72 | -7.32 | -1.57 | -5.86 | 0.81 | -4.24 | -4.46 | -4.70 | 0.14 | -1.66 | -4.98 | -8.06 | -7.12 | -3.78 |
Bud Neck | -4.32 | -11.08 | -10.65 | 1.24 | -0.09 | 1.61 | -9.78 | -6.14 | -7.61 | 6.19 | -1.56 | -12.55 | -10.21 | -7.50 | 4.74 |
Bud Site | -3.34 | -9.65 | -9.06 | -4.48 | -5.31 | 0.74 | -5.28 | -6.02 | -6.52 | -2.28 | -1.20 | -9.49 | -10.64 | -9.82 | -5.67 |
Cell Periphery | -4.71 | 11.21 | -6.57 | -0.05 | -14.90 | 8.66 | 6.65 | -6.85 | -12.93 | -11.65 | 6.83 | 13.93 | -9.43 | -14.57 | -19.41 |
Cytoplasm | 9.31 | 1.73 | 6.63 | -5.43 | 6.10 | -13.99 | 4.37 | 11.22 | 24.92 | 7.45 | -9.13 | 2.33 | 11.57 | 20.06 | 10.59 |
Cytoplasmic Foci | -5.89 | 3.87 | 4.22 | 6.88 | 1.19 | 4.25 | -1.23 | 5.53 | 2.44 | 6.47 | -0.22 | 2.77 | 7.26 | 6.84 | 5.48 |
Eisosomes | -3.68 | -0.20 | -6.66 | 0.77 | -6.64 | 5.96 | -4.66 | -4.54 | -7.60 | 0.84 | 1.32 | -3.42 | -7.65 | -8.04 | -4.51 |
Endoplasmic Reticulum | 1.99 | 1.91 | 2.31 | 0.67 | 1.28 | 3.76 | 7.42 | 7.50 | 2.07 | 0.08 | 3.41 | 4.57 | 5.32 | 1.57 | 1.56 |
Endosome | -2.44 | -2.34 | 0.27 | 2.41 | 2.31 | 5.74 | 0.18 | 0.11 | -2.43 | 0.01 | 3.84 | -1.87 | 0.45 | -1.90 | 1.78 |
Golgi | -4.84 | 16.95 | 16.39 | 11.64 | -0.52 | 11.35 | 18.48 | 18.99 | 9.57 | 3.27 | 8.11 | 26.22 | 25.56 | 15.85 | 1.49 |
Lipid Particles | -5.30 | 4.81 | -5.70 | 0.75 | -9.92 | 7.08 | 0.67 | -9.65 | -14.03 | -10.18 | 4.43 | 5.44 | -10.57 | -13.89 | -14.81 |
Mitochondria | 0.49 | 0.72 | 0.68 | 0.30 | -0.14 | 1.19 | -1.72 | -0.66 | -0.98 | 0.24 | 1.06 | 0.29 | 0.30 | -1.39 | 0.08 |
Mitotic Spindle | -4.14 | 0.61 | 1.51 | 4.77 | 0.91 | 0.96 | -0.70 | 3.53 | 1.84 | 3.43 | -0.50 | 0.49 | 3.89 | 2.89 | 3.34 |
None | 0.79 | 1.43 | -0.07 | -0.58 | -1.16 | -2.89 | 0.76 | 1.86 | 3.99 | 1.84 | -3.07 | 1.61 | 1.20 | 3.58 | -0.31 |
Nuclear Periphery | -5.44 | 8.46 | 6.26 | 6.97 | -2.93 | 9.70 | 1.97 | 9.84 | -0.03 | 9.48 | 5.91 | 8.12 | 11.75 | 5.24 | 5.11 |
Nuclear Periphery Foci | -3.65 | 9.87 | 9.51 | 6.86 | -0.71 | 8.00 | 6.90 | 11.59 | 3.53 | 6.03 | 4.97 | 13.33 | 15.31 | 7.86 | 3.02 |
Nucleolus | -1.03 | -6.77 | -6.37 | 0.91 | -0.85 | -0.96 | -7.28 | -4.22 | -2.16 | 0.36 | -1.18 | -8.98 | -7.49 | 0.43 | -0.43 |
Nucleus | -2.79 | -7.07 | -4.22 | 1.60 | 2.27 | -1.32 | -13.65 | -2.52 | -1.79 | 10.94 | -3.29 | -14.02 | -4.42 | -1.11 | 9.12 |
Peroxisomes | -1.01 | -5.82 | -6.69 | 0.20 | -4.27 | -0.30 | -1.10 | -5.72 | -5.10 | -0.63 | -0.97 | -2.88 | -8.77 | -3.42 | -2.88 |
Vacuole | -6.82 | -19.31 | -16.63 | -3.05 | -1.24 | 7.31 | -16.62 | -24.04 | -28.87 | -8.82 | 3.46 | -25.03 | -27.97 | -29.52 | -7.60 |
Vacuole Periphery | -1.00 | 11.23 | 11.37 | 7.94 | 1.45 | 12.45 | 15.47 | 16.68 | 5.99 | 2.32 | 12.02 | 18.99 | 19.76 | 8.08 | 2.74 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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