Standard name
Human Ortholog
Description Protein involved in modulation of Ime2p activity during meiosis; appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0.05 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.28 0.08 0.07 0.16 0.18 0.16 0.23 0.16 0.16 0.15 0.1 0.08 0.08 0.15 0.23 – – – 0.24 0.07 0 0.06 0.11 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.05 0 0.14 0.23 0.16 0.32 0.42 0.42 0.42 0.48 0.53 0.54 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.16 0.5 0.76 0.68 0.74 0.6 0.54 0.62 0.66 0.61 0.69 0.65 0.85 0.72 0.7 – – – 0.58 0.79 0.69 0.65 0.57 0.43
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0.05 0
Vac/Vac Membrane 0.61 0.55 0.14 0.12 0.07 0.07 0.05 0.07 0 0 0.06 0 0.07 0.12 0.08 – – – 0.08 0.08 0.17 0.11 0.1 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 2
Bud 0 0 0 0 0 0 1 1 0 1 1 1 0 0 0 – – – 2 3 1 6 10 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 6
Bud Site 0 0 0 0 0 1 1 3 1 1 2 3 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 21 14 3 9 14 15 35 26 15 21 9 11 15 39 72 – – – 26 13 2 9 23 22
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 1 0 0 0 1 0 – – – 0 0 0 0 0 4
Endosome 5 2 0 0 0 0 0 0 0 0 0 0 0 3 4 – – – 1 1 0 3 6 7
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 1 0 1 1 1
Mitochondria 4 4 6 13 12 30 63 66 38 66 48 73 0 3 3 – – – 1 1 0 1 2 2
Nucleus 12 89 32 38 56 57 81 99 60 83 62 88 152 190 220 – – – 61 151 49 100 121 88
Nuclear Periphery 0 0 0 0 0 1 2 0 2 1 0 1 0 0 0 – – – 0 0 0 0 1 1
Nucleolus 0 0 0 0 1 1 3 3 2 5 5 8 1 2 3 – – – 1 1 0 6 8 6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 1
SpindlePole 0 1 0 0 0 0 1 0 0 0 0 0 1 1 0 – – – 1 1 0 1 9 6
Vac/Vac Membrane 46 98 6 7 5 7 8 11 2 6 5 3 12 32 26 – – – 8 16 12 16 21 34
Unique Cell Count 76 178 42 56 76 95 149 159 91 137 90 136 178 264 315 107 193 71 154 214 208
Labelled Cell Count 89 209 47 67 89 112 195 209 120 185 132 188 181 271 328 107 193 71 154 214 208


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 7.6 5.3 5.4 5.8 5.4 4.9 4.9 5.2 5.1 4.9 5.0 8.4 8.4 7.7 – – – 6.7 7.2 7.8
Std Deviation (1e-4) 0.9 1.3 0.9 1.2 1.4 1.4 1.4 1.1 1.3 1.2 1.1 1.6 1.8 2.4 2.2 – – – 1.3 1.6 1.4
Intensity Change (Log2) – – – 0.03 0.14 0.04 -0.1 -0.1 -0.0 -0.05 -0.1 -0.07 0.67 0.67 0.56 – – – 0.36 0.45 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 1.3 1.7 1.4 2.3 1.5 1.5 1.4 0 0 0 1.3 2.3 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 1.1 0.2 2.1 3.3 3.3 3.1 3.9 4.2 4.5 0 0 0 – – –
Nucleus -0.9 -0.3 -1.8 -2.5 -1.7 -1.2 -1.8 -0.9 -1.4 1.5 -0.6 -0.8 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole -0.3 0 0 0 0 0 0 0 0 0 -0.4 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9636 4.4872 4.2089 3.5023 4.1378 4.1844 3.4414 3.7557 3.8131 3.136 3.517 3.6837 5.3066 5.9409 4.932 4.5576 4.6061 5.0192
Actin 0.0279 0 0.0204 0.0005 0.005 0.014 0.0032 0.015 0.003 0.0088 0.0001 0.0012 0.1203 0.0076 0.0124 0 0.0001 0.0088
Bud 0.0008 0.0001 0.0003 0.001 0.0002 0.0005 0.0003 0.0001 0.0001 0.0024 0.0001 0.0001 0.0014 0.0007 0.0001 0 0.0003 0.0001
Bud Neck 0.0023 0.0002 0.0009 0.0004 0.0059 0.0038 0.0006 0.0003 0.0006 0.0142 0.0004 0.0009 0.0137 0.0002 0.0003 0.0008 0.0022 0.0014
Bud Periphery 0.0019 0 0.0006 0.0052 0.0008 0.0011 0.0004 0.0001 0.0001 0.0037 0 0.0001 0.0016 0.0009 0.0001 0 0.0007 0.0001
Bud Site 0.0022 0.0002 0.0008 0.0005 0.0004 0.0004 0.0014 0.0006 0.0002 0.0085 0.0001 0.0002 0.0102 0.0008 0.002 0.0001 0.0016 0.0002
Cell Periphery 0.0004 0 0.0001 0.0008 0.0004 0.0001 0.0001 0 0 0.0005 0 0 0.0006 0.0004 0 0 0.001 0
Cytoplasm 0.0197 0.0127 0.0058 0.031 0.0007 0.0021 0.0254 0.0124 0.0083 0.0071 0.001 0.0017 0.0403 0.0499 0.0204 0.0014 0.0009 0.0011
Cytoplasmic Foci 0.0102 0.0001 0.005 0.0002 0.0008 0.0009 0.0276 0.0054 0.0012 0.0264 0.0069 0.003 0.0188 0.0031 0.0079 0 0.0063 0.0018
Eisosomes 0.0004 0 0.0001 0 0.0001 0.0001 0 0 0 0.0001 0 0 0.0009 0.0006 0 0 0 0
Endoplasmic Reticulum 0.0124 0.0002 0.0025 0.001 0.011 0.0018 0.0018 0.0031 0.0021 0.002 0.0001 0.0001 0.0194 0.0021 0.0002 0 0.0001 0.0001
Endosome 0.0335 0.0002 0.0099 0.0009 0.0061 0.0077 0.0321 0.0122 0.0056 0.0294 0.0488 0.0021 0.0259 0.0055 0.0004 0 0.0167 0.0008
Golgi 0.0081 0 0.0036 0.0001 0.003 0.0048 0.0188 0.0064 0.0007 0.0131 0.0301 0.0039 0.0066 0.008 0.0015 0 0.006 0.0019
Lipid Particles 0.0111 0 0.0043 0.0001 0.0125 0.0021 0.0168 0.0024 0.0005 0.0097 0.0012 0.0011 0.0044 0.0003 0.0008 0 0.0164 0.0018
Mitochondria 0.0062 0.0001 0.0054 0.0385 0.0602 0.0094 0.0034 0.0013 0.0004 0.0114 0.002 0.0036 0.0044 0.0247 0.0027 0.0001 0.0233 0.0004
None 0.0193 0.0086 0.0024 0.0004 0.0001 0.0003 0.006 0.001 0.0007 0.0009 0.0001 0.0001 0.0656 0.0494 0.0005 0.0001 0.0001 0.0001
Nuclear Periphery 0.0514 0.0042 0.0091 0.0049 0.031 0.0062 0.0084 0.0061 0.0079 0.0039 0.0032 0.0038 0.0315 0.0038 0.0047 0.002 0.0017 0.0015
Nucleolus 0.0051 0.0024 0.0025 0.0032 0.005 0.0059 0.0034 0.0022 0.0019 0.0078 0.0042 0.0049 0.0719 0.0034 0.001 0.0023 0.009 0.0075
Nucleus 0.749 0.9691 0.911 0.8995 0.8477 0.9265 0.8268 0.9265 0.9624 0.8248 0.8937 0.9656 0.371 0.8346 0.9286 0.9925 0.884 0.9648
Peroxisomes 0.0041 0 0.0084 0.0001 0.0038 0.0016 0.0107 0.0018 0.0002 0.0084 0.0006 0.0061 0.0053 0.0002 0.0154 0 0.0046 0.0032
Punctate Nuclear 0.0221 0.0013 0.005 0.0013 0.001 0.0092 0.0078 0.0018 0.0035 0.0099 0.0063 0.0013 0.1769 0.0019 0.0007 0.0003 0.0012 0.004
Vacuole 0.0084 0.0005 0.0012 0.0054 0.0025 0.0007 0.004 0.0009 0.0004 0.006 0.0006 0.0002 0.0078 0.0015 0.0001 0.0001 0.0204 0.0002
Vacuole Periphery 0.0034 0.0001 0.0007 0.0049 0.0017 0.0006 0.0011 0.0002 0.0002 0.001 0.0005 0.0001 0.0015 0.0003 0 0 0.0033 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.043 3.4978 7.7865 11.952 7.1023 7.8212 10.6098 13.367 11.5876 10.942
Translational Efficiency 1.2483 2.1771 1.1293 0.8348 1.5181 1.5598 1.0264 0.843 1.2209 1.021

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in modulation of Ime2p activity during meiosis; appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation
Localization
Cell Percentages nucleus (47%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ids2

Ids2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ids2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available