Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0.06 0.05 0.06 0.07 0.05 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.26 0.43 0.26 0.15 0.41 0.14 0.12 0.09 0.11 0.08 0.05 0.08 0.63 0.75 0.82 0.34 0.28 0.35 0.11 0.24 0.05 0.1 0.11 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.08 0.06 0.16 0.12 0.21 0.11 0.06 0.09 0.07 0.08 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 0.47 0.64 0.86 0.61 0.89 0.9 0.86 0.89 0.93 0.95 0.93 0.11 0.05 0 0.16 0.16 0.11 0.63 0.51 0.63 0.66 0.58 0.55
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0.14 0 0 0.08 0 0 0
SpindlePole 0 0 0 0 0 0 0.05 0.09 0.07 0 0 0.06 0.08 0 0 0.16 0.17 0.17 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.12 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0.1 0.12 0.14 0 0.09 0 0.06 0.07 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 1 0 0 0 1 0 0 0 0 9 2 4 0 13 2 2 0 7 6 10 18
Bud 5 2 12 6 7 11 40 27 28 32 25 28 3 1 2 7 13 8 0 1 2 2 9 12
Bud Neck 0 0 3 0 2 0 2 3 1 2 0 2 3 3 7 1 7 0 2 1 1 6 8 10
Bud Site 0 0 0 0 1 9 8 9 13 14 15 20 0 0 0 0 2 0 – – – – – –
Cell Periphery 0 3 6 6 1 3 3 3 2 2 4 2 11 15 7 12 13 10 0 0 1 1 2 1
Cytoplasm 62 39 58 34 128 57 83 53 50 40 26 46 317 459 573 76 69 67 28 31 12 46 71 80
Endoplasmic Reticulum 3 2 3 2 2 2 2 2 2 3 1 1 16 4 5 17 15 7 2 0 2 2 3 4
Endosome 0 2 9 0 11 3 3 4 0 0 0 0 53 52 45 36 29 39 30 7 24 29 49 49
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 2 1 0 2 4 1 2 1 2 4 4 12
Mitochondria 193 43 142 197 192 361 598 485 388 445 482 553 53 29 23 36 39 20 165 65 168 298 362 347
Nucleus 2 0 0 0 5 6 16 10 5 6 5 7 2 0 2 7 1 2 0 0 1 1 4 4
Nuclear Periphery 1 0 3 1 0 1 6 5 8 9 6 10 0 0 1 0 1 0 1 0 0 0 3 5
Nucleolus 1 0 0 0 2 6 9 6 5 3 5 7 0 0 1 0 0 0 0 0 0 0 3 2
Peroxisomes 1 0 16 5 12 1 8 10 5 5 1 5 19 11 12 25 16 26 7 1 20 6 9 13
SpindlePole 8 1 9 9 10 14 35 52 29 21 16 36 38 24 24 36 42 33 4 3 6 12 27 21
Vac/Vac Membrane 11 11 3 4 12 17 15 10 18 13 21 21 27 57 31 22 30 27 7 11 4 25 41 29
Unique Cell Count 241 91 221 230 316 407 664 566 438 481 506 595 503 615 699 223 243 190 262 128 266 454 624 627
Labelled Cell Count 288 103 265 265 385 491 828 680 554 595 607 738 553 658 737 277 294 242 262 128 266 454 624 627


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.5 3.9 3.5 3.7 3.3 3.2 3.3 3.1 3.1 2.9 3.0 5.3 5.3 5.1 5.7 5.8 5.5 5.6 5.6 6.0
Std Deviation (1e-4) 0.5 1.0 0.9 1.2 1.2 0.8 1.0 1.0 0.9 0.8 0.7 0.7 1.2 1.0 1.0 1.4 1.2 1.2 1.0 1.4 1.5
Intensity Change (Log2) – – – -0.13 -0.05 -0.25 -0.26 -0.23 -0.32 -0.33 -0.42 -0.39 0.45 0.46 0.39 0.56 0.58 0.51 0.53 0.54 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.9 4.6 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7725 2.2785 2.3869 2.0521 2.8297 2.5078 0.1915 0.3086 0.3322 0.2706 1.0035 0.2591 -0.7848 -0.6805 -0.8853 -0.3492 -0.5899 -0.9499
Actin 0.0418 0.0167 0.0262 0.0239 0.0183 0.0111 0.0257 0.016 0.0135 0.0213 0.0116 0.0104 0.0262 0.0148 0.0238 0.0072 0.004 0.0196
Bud 0.0015 0.0127 0.0024 0.0011 0.0094 0.0043 0.0022 0.0031 0.0023 0.0035 0.0023 0.0017 0.0017 0.0021 0.0039 0.002 0.0054 0.0011
Bud Neck 0.0035 0.0026 0.0032 0.001 0.0009 0.0119 0.0163 0.0016 0.0055 0.0019 0.0009 0.0062 0.0027 0.0015 0.0047 0.0006 0.001 0.0175
Bud Periphery 0.0052 0.0125 0.0088 0.0024 0.0265 0.0079 0.0048 0.0048 0.0042 0.0073 0.0041 0.004 0.0034 0.0035 0.0059 0.0059 0.0147 0.0032
Bud Site 0.0058 0.0479 0.0269 0.0025 0.0233 0.0027 0.0066 0.0421 0.0185 0.0079 0.0029 0.0029 0.0078 0.0292 0.0143 0.0013 0.0014 0.0014
Cell Periphery 0.0016 0.0034 0.0042 0.0003 0.0163 0.0015 0.0025 0.0019 0.001 0.0009 0.0006 0.0017 0.0014 0.003 0.0011 0.0008 0.0004 0.0017
Cytoplasm 0.0259 0.0676 0.0313 0.022 0.0164 0.0506 0.0474 0.1153 0.1006 0.0691 0.0381 0.095 0.0324 0.0987 0.0658 0.0761 0.0307 0.0676
Cytoplasmic Foci 0.0995 0.1057 0.0795 0.0681 0.0662 0.0974 0.0964 0.1067 0.0922 0.0887 0.1136 0.084 0.0877 0.0928 0.0782 0.0494 0.0667 0.0708
Eisosomes 0.0026 0.0007 0.0019 0.0004 0.0024 0.0004 0.0006 0.0008 0.0004 0.0005 0.0003 0.0002 0.0006 0.0011 0.0007 0.0005 0.0001 0.0004
Endoplasmic Reticulum 0.0062 0.0038 0.0043 0.0028 0.0192 0.0032 0.0072 0.0076 0.0068 0.0041 0.0044 0.0101 0.0087 0.0113 0.0078 0.0185 0.0019 0.0063
Endosome 0.1128 0.1317 0.0994 0.1324 0.1104 0.1289 0.2783 0.1934 0.2092 0.1559 0.2705 0.1823 0.2219 0.184 0.1342 0.2167 0.3238 0.2107
Golgi 0.0573 0.0537 0.0564 0.0666 0.0443 0.0431 0.0745 0.058 0.0592 0.0645 0.0711 0.0743 0.0652 0.0609 0.0566 0.033 0.1676 0.0869
Lipid Particles 0.0668 0.024 0.0188 0.0594 0.0182 0.0535 0.0301 0.0213 0.0222 0.0374 0.0203 0.0159 0.0403 0.026 0.0242 0.0181 0.0059 0.0313
Mitochondria 0.3376 0.3461 0.4277 0.3212 0.2695 0.3688 0.2737 0.1885 0.3201 0.4679 0.3213 0.3979 0.2928 0.1919 0.3304 0.2548 0.2995 0.3771
None 0.1335 0.0914 0.1155 0.1839 0.1916 0.1169 0.0462 0.1663 0.0843 0.0296 0.0464 0.0495 0.1461 0.2125 0.1899 0.1887 0.0018 0.0482
Nuclear Periphery 0.0068 0.0029 0.0038 0.0026 0.0033 0.0026 0.0147 0.003 0.0042 0.0013 0.0146 0.0065 0.0032 0.0059 0.0034 0.0081 0.0009 0.0029
Nucleolus 0.0047 0.0043 0.0015 0.0026 0.0029 0.0012 0.0016 0.0017 0.0012 0.0005 0.0014 0.0013 0.0013 0.0031 0.0021 0.0023 0.0003 0.0008
Nucleus 0.0041 0.0032 0.002 0.0013 0.0048 0.0022 0.0045 0.0039 0.0021 0.0011 0.0032 0.0064 0.0019 0.004 0.0035 0.0037 0.0012 0.0015
Peroxisomes 0.0574 0.0447 0.0695 0.0869 0.0786 0.0743 0.0201 0.0223 0.0235 0.0179 0.0145 0.0205 0.021 0.0107 0.0209 0.036 0.0061 0.0249
Punctate Nuclear 0.0119 0.0026 0.0033 0.0038 0.0627 0.0023 0.0048 0.0015 0.0018 0.0013 0.0385 0.0029 0.0036 0.0027 0.0041 0.0022 0.0002 0.0043
Vacuole 0.0084 0.0172 0.0087 0.0098 0.0088 0.0105 0.0296 0.0333 0.0191 0.0101 0.0109 0.0166 0.0222 0.0317 0.0167 0.0445 0.0482 0.0135
Vacuole Periphery 0.005 0.0047 0.0045 0.0052 0.006 0.0049 0.0122 0.007 0.0083 0.0074 0.0086 0.0098 0.0079 0.0085 0.0078 0.0299 0.0183 0.0083

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.3611 5.1508 2.5481 4.6589 4.9804 3.8391 5.642 4.9796 7.798 7.9136
Translational Efficiency 1.2246 0.7472 1.292 0.807 0.7801 1.0509 1.095 0.9541 0.6804 0.5847

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1542 443 2127 433 945 856 1211 1205 2487 1299 3338 1638

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 685.94 723.37 782.16 803.92 670.62 709.73 816.39 815.83 680.12 714.38 794.58 812.68
Standard Deviation 90.24 91.44 84.92 100.58 86.97 90.28 94.75 108.56 89.32 90.91 90.13 106.64
Intensity Change Log 2 — 0.076651 0.189381 0.228970 — 0.081775 0.283763 0.282773 — 0.079186 0.236811 0.255818

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000706 0.002282 0.001692 0.002239 0.000827 0.001475 0.001332 0.004237 0.000752 0.001750 0.001561 0.003709
Bud Neck 0.026714 0.030494 0.048026 0.018727 0.027555 0.046569 0.024958 0.022139 0.027034 0.041087 0.039657 0.021237
Bud Site 0.015885 0.038283 0.032885 0.050522 0.022702 0.030135 0.017392 0.046537 0.018475 0.032914 0.027264 0.047591
Cell Periphery 0.000397 0.000500 0.000151 0.000282 0.000416 0.000420 0.000222 0.000300 0.000404 0.000447 0.000177 0.000295
Cytoplasm 0.098191 0.054149 0.312623* 0.210442* 0.141235* 0.094684 0.266828* 0.340103* 0.114547* 0.080860 0.296009* 0.305827*
Cytoplasmic Foci 0.234874* 0.154905 0.083231 0.104830 0.294233* 0.212254* 0.120412 0.093845 0.257429* 0.192696* 0.096720 0.096749
Eisosomes 0.000255 0.000187 0.000078 0.000155 0.000234 0.000221 0.000141 0.000149 0.000247 0.000209 0.000100 0.000151
Endoplasmic Reticulum 0.001926 0.000982 0.003696 0.005518 0.002968 0.001282 0.003100 0.002506 0.002322 0.001179 0.003480 0.003302
Endosome 0.080960 0.109544 0.024353 0.039424 0.075805 0.104050 0.044357 0.019334 0.079002 0.105923 0.031610 0.024645
Golgi 0.094640 0.083978 0.006564 0.008562 0.077279 0.047927 0.007959 0.005418 0.088043 0.060222 0.007070 0.006249
Lipid Particles 0.022798 0.013266 0.004287 0.008331 0.023761 0.018287 0.010734 0.004696 0.023164 0.016575 0.006626 0.005657
Mitochondria 0.284833* 0.286463* 0.026075 0.021401 0.192733* 0.208347* 0.022720 0.018448 0.249837* 0.234987* 0.024858 0.019229
Mitotic Spindle 0.005950 0.022891 0.052259 0.040574 0.007799 0.023300 0.022678 0.024006 0.006653 0.023160 0.041527 0.028386
None 0.002294 0.000903 0.002024 0.003341 0.001809 0.001620 0.003598 0.004514 0.002110 0.001376 0.002595 0.004204
Nuclear Periphery 0.000833 0.000322 0.003560 0.004463 0.000682 0.000702 0.001957 0.001689 0.000775 0.000572 0.002978 0.002422
Nuclear Periphery Foci 0.000361 0.000602 0.001100 0.004806 0.000586 0.000520 0.001574 0.001423 0.000447 0.000548 0.001272 0.002317
Nucleolus 0.002970 0.001117 0.003601 0.003022 0.002203 0.003439 0.005150 0.002869 0.002679 0.002647 0.004163 0.002910
Nucleus 0.024583 0.018855 0.259742* 0.168603* 0.028502 0.042162 0.301610* 0.197899* 0.026072 0.034214 0.274931* 0.190155*
Peroxisomes 0.048402 0.126374* 0.027657 0.028982 0.037014 0.076473 0.030161 0.035362 0.044075 0.093491 0.028565 0.033675
Vacuole 0.035590 0.043283 0.102746 0.269278* 0.047205 0.070711 0.108657 0.171419 0.040003 0.061357 0.104891 0.197288
Vacuole Periphery 0.016838 0.010620 0.003653 0.006499 0.014452 0.015422 0.004460 0.003107 0.015931 0.013784 0.003946 0.004003

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.40 -3.42 -1.82 1.20 0.06 -2.52 -2.18 -2.65 -1.70 -1.98 -4.35 -4.01 -3.08 -1.15 -1.70
Bud Neck -0.87 -6.45 4.34 3.76 10.65 -4.27 1.19 3.62 6.99 2.65 -4.32 -5.10 5.19 7.81 10.69
Bud Site -4.46 -6.33 -3.53 0.42 -0.99 -2.03 2.52 -2.87 -0.68 -4.90 -5.09 -3.95 -5.32 -0.19 -2.96
Cell Periphery -1.19 7.49 3.42 3.04 -3.12 -0.07 2.76 2.45 3.75 -0.49 -0.80 6.72 4.35 4.83 -2.78
Cytoplasm 5.63 -27.27 -9.24 -12.09 7.18 5.18 -11.87 -16.47 -22.75 -4.68 5.71 -28.57 -22.58 -26.19 0.42
Cytoplasmic Foci 7.83 26.29 19.89 7.23 -0.42 8.04 22.37 28.77 17.61 7.18 8.99 34.40 35.35 18.67 4.14
Eisosomes 3.22 13.50 7.67 2.57 -8.09 0.68 6.40 6.68 7.19 1.06 2.78 14.84 10.38 7.33 -5.45
Endoplasmic Reticulum 1.92 -4.49 -5.52 -7.64 -2.66 4.13 -1.16 -0.22 -6.54 1.36 3.63 -4.48 -4.09 -9.94 0.23
Endosome -3.53 16.97 11.64 10.43 -1.34 -4.24 7.89 15.70 16.62 8.48 -5.33 18.26 22.34 19.96 6.30
Golgi 1.53 21.84 20.21 11.84 0.31 5.21 15.12 15.92 12.94 3.17 6.48 26.53 26.89 17.47 2.67
Lipid Particles 3.30 11.62 9.06 2.57 -3.57 1.94 6.57 10.27 7.73 6.14 3.34 13.28 14.68 8.01 3.21
Mitochondria 0.08 31.70* 30.83* 17.30 2.71 -1.06 19.66 20.78 19.38 2.64 1.67 36.89 37.92* 25.81 4.63
Mitotic Spindle -5.04 -12.60 -4.12 -1.29 2.23 -4.64 -5.08 -4.13 1.00 1.22 -6.81 -13.45 -6.20 0.13 4.93
None 2.93 0.80 -0.16 -1.49 -0.50 -0.09 -2.02 -1.96 -1.72 -0.15 1.20 -0.92 -1.62 -2.31 -1.13
Nuclear Periphery 2.31 -6.41 -4.35 -5.15 -0.42 -0.08 -5.07 -4.07 -4.74 0.07 1.01 -7.76 -6.06 -7.14 1.37
Nuclear Periphery Foci -1.26 -5.34 -3.59 -3.18 -2.76 0.41 -5.05 -4.78 -5.07 0.51 -1.30 -7.28 -5.74 -5.06 -2.55
Nucleolus 4.20 -1.60 0.94 -2.77 2.57 -2.22 -4.03 -0.11 1.82 3.35 -0.54 -3.83 0.63 1.00 3.52
Nucleus 0.99 -39.34 -14.03 -13.88 7.67 -3.09 -31.15 -26.36 -22.53 8.00 -2.82 -50.12 -30.59 -26.92 10.28
Peroxisomes -7.39 7.47 6.58 9.97 1.70 -7.11 2.91 3.90 9.66 1.19 -9.55 7.84 7.80 13.70 1.60
Vacuole -1.87 -20.29 -22.53 -21.13 -16.29 -4.35 -14.48 -25.51 -21.25 -13.65 -5.89 -24.76 -34.61 -29.35 -21.49
Vacuole Periphery 3.22 11.42 9.08 3.77 -0.81 -0.42 7.68 8.58 7.92 2.24 1.56 13.78 13.14 8.83 1.66
Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene
Localization
Cell Percentages mitochondrion (73%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Smt1

Smt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available