Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 2 | 5 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 4 | 9 | 21 | 21 | 51 | – | 98 | 96 | 102 | 106 | 104 | 20 | 33 | 15 | 13 | 1 | 3 | 1 | 1 | 4 | 0 | 5 | 3 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 2 | 1 | – | 0 | 0 | 2 | 2 | 2 | 2 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 1 | 0 | 0 | 2 | 1 | – | 1 | 0 | 2 | 0 | 0 | 1 | 14 | 27 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 8 | 7 |
Golgi | 0 | 2 | 2 | 1 | 4 | 0 | – | 4 | 0 | 2 | 1 | 0 | 13 | 7 | 2 | 3 | 1 | 1 | 0 | 0 | 1 | 14 | 12 | 14 |
Mitochondria | 7 | 3 | 24 | 9 | 16 | 20 | – | 16 | 6 | 11 | 17 | 16 | 5 | 3 | 1 | 4 | 1 | 1 | 1 | 0 | 0 | 1 | 4 | 3 |
Nucleus | 27 | 25 | 39 | 41 | 34 | 68 | – | 148 | 96 | 116 | 55 | 46 | 6 | 12 | 13 | 17 | 4 | 2 | 14 | 11 | 12 | 1 | 6 | 6 |
Nuclear Periphery | 1 | 0 | 3 | 1 | 1 | 0 | – | 3 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 1 |
Nucleolus | 5 | 3 | 3 | 2 | 10 | 4 | – | 11 | 4 | 4 | 7 | 1 | 9 | 7 | 6 | 2 | 3 | 0 | 2 | 1 | 2 | 1 | 7 | 5 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 103 | 121 | 133 | 146 | 288 | 218 | – | 341 | 293 | 434 | 244 | 293 | 128 | 145 | 164 | 44 | 32 | 17 | 123 | 133 | 169 | 55 | 119 | 144 |
Unique Cell Count | 139 | 142 | 181 | 194 | 347 | 325 | 517 | 427 | 590 | 353 | 413 | 161 | 195 | 200 | 73 | 39 | 23 | 146 | 151 | 205 | 82 | 181 | 197 | |
Labelled Cell Count | 149 | 159 | 213 | 222 | 380 | 364 | 622 | 496 | 675 | 434 | 463 | 187 | 221 | 234 | 88 | 44 | 24 | 146 | 151 | 205 | 82 | 181 | 197 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 28.3 | 35.4 | 19.9 | 24.9 | 35.2 | 30.7 | – | 28.8 | 28.1 | 28.6 | 25.9 | 25.0 | 24.0 | 17.7 | 14.9 | 56.4 | 52.8 | 54.1 | 23.0 | 27.1 | 26.6 |
Std Deviation (1e-4) | 11.8 | 12.5 | 8.2 | 10.1 | 14.0 | 16.5 | – | 14.6 | 15.5 | 14.7 | 12.9 | 12.8 | 10.8 | 7.8 | 7.1 | 33.8 | 22.3 | 28.3 | 9.9 | 13.6 | 11.8 |
Intensity Change (Log2) | – | – | – | 0.32 | 0.82 | 0.63 | – | 0.53 | 0.5 | 0.52 | 0.38 | 0.33 | 0.27 | -0.17 | -0.42 | 1.5 | 1.41 | 1.44 | 0.21 | 0.45 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.1 | 0.5 | 3.6 | – | 4.5 | 5.2 | 4.1 | 6.7 | 5.8 | 2.5 | 3.7 | 1.0 | 3.3 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 5.1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.9 | -3.6 | -2.7 | – | -5.1 | -6.2 | -6.4 | -3.5 | -4.2 | -3.4 | -4.4 | -5.0 | -1.8 | 0 | 0 |
Nucleus | -0.1 | -3.7 | -0.2 | – | 1.9 | 0.3 | -0.6 | -1.7 | -3.3 | -4.9 | -4.4 | -4.3 | 0.3 | -1.6 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.4 | 2.6 | -1.5 | – | -1.9 | -1.2 | 0 | -1.0 | -0.6 | 1.3 | 0.2 | 2.0 | -2.1 | 1.1 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.2912 | 24.8568 | 20.9217 | 20.3554 | 17.6349 | 23.0594 | 14.1553 | 23.5251 | 19.7756 | 18.5567 | 17.6232 | 23.7671 | 15.3928 | 27.2499 | 24.3457 | 21.2749 | 21.1072 | 28.7351 |
Actin | 0.0196 | 0 | 0.0004 | 0.0065 | 0.0001 | 0 | 0.0116 | 0 | 0.003 | 0.0017 | 0.0182 | 0 | 0.0084 | 0.0001 | 0.0009 | 0 | 0.0002 | 0.0001 |
Bud | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0007 | 0.0002 | 0.0003 | 0.0015 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0001 |
Bud Neck | 0.0036 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0004 | 0.0004 | 0.0007 | 0.0003 | 0.0003 |
Bud Periphery | 0.0013 | 0.0003 | 0.0007 | 0.0005 | 0.0005 | 0.0003 | 0.0008 | 0.0006 | 0.0005 | 0.0007 | 0.0021 | 0.0004 | 0.0006 | 0.0005 | 0.0008 | 0.0011 | 0.001 | 0.0005 |
Bud Site | 0.0036 | 0.0002 | 0.0003 | 0.0013 | 0.0003 | 0 | 0.0011 | 0.0004 | 0.0002 | 0.0007 | 0.0022 | 0.0001 | 0.0016 | 0.0009 | 0.0004 | 0.0006 | 0.0005 | 0.0001 |
Cell Periphery | 0.0093 | 0.0087 | 0.0083 | 0.0075 | 0.003 | 0.0126 | 0.0113 | 0.013 | 0.0126 | 0.0068 | 0.0076 | 0.0143 | 0.0138 | 0.0169 | 0.0114 | 0.0179 | 0.0128 | 0.0158 |
Cytoplasm | 0.0101 | 0.0024 | 0.0021 | 0.0006 | 0.0005 | 0.0001 | 0.0036 | 0.0005 | 0.0002 | 0.001 | 0.0022 | 0.0002 | 0.011 | 0.002 | 0.0006 | 0.0003 | 0.004 | 0.0002 |
Cytoplasmic Foci | 0.0228 | 0.0012 | 0.0031 | 0.0103 | 0.003 | 0.0001 | 0.0157 | 0.0002 | 0.001 | 0.0142 | 0.0129 | 0.0001 | 0.0157 | 0.003 | 0.0067 | 0.0008 | 0.0061 | 0.0035 |
Eisosomes | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0051 | 0.0003 | 0.0009 | 0.0011 | 0.0002 | 0.0002 | 0.0008 | 0.0003 | 0.0007 | 0.0014 | 0.0017 | 0.0004 | 0.0035 | 0.0003 | 0.0008 | 0.0002 | 0.0023 | 0.0003 |
Endosome | 0.0601 | 0.0098 | 0.0241 | 0.0068 | 0.0527 | 0.0035 | 0.0216 | 0.0058 | 0.0144 | 0.0422 | 0.0587 | 0.0035 | 0.0543 | 0.0066 | 0.0242 | 0.0116 | 0.0486 | 0.0082 |
Golgi | 0.0127 | 0.0028 | 0.0036 | 0.0032 | 0.0241 | 0.0002 | 0.0137 | 0.0004 | 0.0035 | 0.0041 | 0.0225 | 0.0001 | 0.0087 | 0.0005 | 0.0067 | 0.0004 | 0.0072 | 0.0065 |
Lipid Particles | 0.0385 | 0.0105 | 0.0174 | 0.0278 | 0.0039 | 0.0075 | 0.0223 | 0.0037 | 0.0163 | 0.0183 | 0.0178 | 0.0022 | 0.0371 | 0.0037 | 0.0129 | 0.0094 | 0.0189 | 0.0224 |
Mitochondria | 0.0201 | 0.0049 | 0.0218 | 0.0569 | 0.0118 | 0.003 | 0.0247 | 0.0024 | 0.0383 | 0.0318 | 0.067 | 0.0012 | 0.0471 | 0.0023 | 0.0329 | 0.0024 | 0.0101 | 0.032 |
None | 0.0035 | 0.0014 | 0.0001 | 0.0012 | 0 | 0 | 0.0008 | 0 | 0.0002 | 0.0014 | 0.0001 | 0 | 0.0044 | 0.0002 | 0.0002 | 0 | 0.0024 | 0 |
Nuclear Periphery | 0.0132 | 0.0012 | 0.0122 | 0.0011 | 0.0006 | 0.0026 | 0.0088 | 0.0022 | 0.0013 | 0.0013 | 0.0013 | 0.0021 | 0.0186 | 0.006 | 0.0017 | 0.0006 | 0.0088 | 0.0007 |
Nucleolus | 0.014 | 0.0042 | 0.0172 | 0.0018 | 0.0013 | 0.0023 | 0.0206 | 0.0034 | 0.006 | 0.0064 | 0.0011 | 0.0016 | 0.0147 | 0.0137 | 0.0048 | 0.0177 | 0.0078 | 0.0038 |
Nucleus | 0.0049 | 0.002 | 0.0038 | 0.0011 | 0.0013 | 0.0014 | 0.01 | 0.0023 | 0.0018 | 0.002 | 0.0007 | 0.0016 | 0.0052 | 0.0037 | 0.0019 | 0.0033 | 0.0046 | 0.0018 |
Peroxisomes | 0.0036 | 0.0001 | 0.0008 | 0.0053 | 0.0011 | 0 | 0.0087 | 0.0001 | 0.0004 | 0.0031 | 0.0052 | 0 | 0.0033 | 0.0003 | 0.0008 | 0.0003 | 0.0007 | 0.0008 |
Punctate Nuclear | 0.0029 | 0.0001 | 0.0001 | 0.0007 | 0 | 0 | 0.0012 | 0 | 0 | 0.0006 | 0.0004 | 0 | 0.0048 | 0.0001 | 0.0002 | 0 | 0.0006 | 0 |
Vacuole | 0.3559 | 0.4824 | 0.4122 | 0.489 | 0.4876 | 0.471 | 0.4143 | 0.5072 | 0.4447 | 0.4563 | 0.3959 | 0.5427 | 0.3741 | 0.5004 | 0.449 | 0.5439 | 0.5291 | 0.5381 |
Vacuole Periphery | 0.3943 | 0.4671 | 0.4702 | 0.3767 | 0.4073 | 0.495 | 0.407 | 0.4565 | 0.4543 | 0.4051 | 0.3805 | 0.4291 | 0.3724 | 0.438 | 0.4424 | 0.3882 | 0.3333 | 0.3648 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 149.5909 | 139.8593 | 149.2999 | 154.4917 | 167.2606 | 150.8797 | 153.1959 | 192.561 | 184.4237 | 166.7941 |
Translational Efficiency | 1.4596 | 1.4255 | 1.588 | 1.7621 | 1.4152 | 2.0226 | 1.6877 | 1.5178 | 1.8047 | 1.4557 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1310 | 1266 | 975 | 1395 | 904 | 1990 | 2177 | 1799 | 2214 | 3256 | 3152 | 3194 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1654.02 | 1994.21 | 1719.50 | 2798.47 | 2736.62 | 1843.29 | 2004.59 | 2566.85 | 2096.06 | 1901.97 | 1916.40 | 2668.01 |
Standard Deviation | 541.90 | 555.80 | 285.54 | 683.40 | 1031.75 | 457.79 | 346.02 | 515.72 | 944.22 | 503.60 | 353.95 | 605.79 |
Intensity Change Log 2 | — | 0.269841 | 0.056012 | 0.758662 | — | -0.570112 | -0.449088 | -0.092396 | — | -0.194264 | -0.237543 | 0.289233 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000183 | 0.000077 | 0.000304 | 0.004606 | 0.000151 | 0.000091 | 0.000690 | 0.004203 | 0.000170 | 0.000086 | 0.000570 | 0.004379 |
Bud Neck | 0.002057 | 0.003314 | 0.004522 | 0.013732 | 0.000960 | 0.002458 | 0.005839 | 0.014847 | 0.001609 | 0.002791 | 0.005432 | 0.014360 |
Bud Site | 0.008201 | 0.004247 | 0.005410 | 0.013303 | 0.000711 | 0.002114 | 0.009840 | 0.019262 | 0.005143 | 0.002943 | 0.008469 | 0.016659 |
Cell Periphery | 0.030280 | 0.016144 | 0.003348 | 0.250824* | 0.034753 | 0.012142 | 0.028699 | 0.167145* | 0.032106 | 0.013698 | 0.020857 | 0.203693* |
Cytoplasm | 0.002542 | 0.000335 | 0.000538 | 0.000649 | 0.000795 | 0.000488 | 0.000183 | 0.001984 | 0.001829 | 0.000429 | 0.000293 | 0.001401 |
Cytoplasmic Foci | 0.001454 | 0.002272 | 0.011866 | 0.000892 | 0.001912 | 0.000912 | 0.003318 | 0.004429 | 0.001641 | 0.001441 | 0.005962 | 0.002884 |
Eisosomes | 0.000252 | 0.001458 | 0.000062 | 0.003175 | 0.000816 | 0.001571 | 0.000150 | 0.001603 | 0.000482 | 0.001527 | 0.000122 | 0.002289 |
Endoplasmic Reticulum | 0.002597 | 0.001462 | 0.001232 | 0.078750 | 0.000649 | 0.001612 | 0.018913 | 0.057382 | 0.001802 | 0.001553 | 0.013444 | 0.066715 |
Endosome | 0.006135 | 0.002527 | 0.042828 | 0.026486 | 0.000451 | 0.003444 | 0.142263 | 0.075911 | 0.003814 | 0.003087 | 0.111505 | 0.054325 |
Golgi | 0.005767 | 0.001600 | 0.034685 | 0.017807 | 0.000194 | 0.000752 | 0.042010 | 0.035493 | 0.003491 | 0.001082 | 0.039744 | 0.027769 |
Lipid Particles | 0.001117 | 0.001184 | 0.007936 | 0.202388* | 0.000290 | 0.001494 | 0.035768 | 0.212508* | 0.000779 | 0.001374 | 0.027159 | 0.208088* |
Mitochondria | 0.001301 | 0.001598 | 0.001969 | 0.001008 | 0.000446 | 0.003482 | 0.001119 | 0.001543 | 0.000952 | 0.002749 | 0.001382 | 0.001309 |
Mitotic Spindle | 0.000030 | 0.000109 | 0.000420 | 0.000384 | 0.000005 | 0.000450 | 0.000495 | 0.000596 | 0.000020 | 0.000318 | 0.000472 | 0.000504 |
None | 0.000931 | 0.000196 | 0.000076 | 0.000070 | 0.000390 | 0.000162 | 0.000027 | 0.000068 | 0.000710 | 0.000175 | 0.000042 | 0.000069 |
Nuclear Periphery | 0.000081 | 0.000302 | 0.001807 | 0.002253 | 0.000021 | 0.000339 | 0.000345 | 0.000945 | 0.000056 | 0.000324 | 0.000797 | 0.001516 |
Nuclear Periphery Foci | 0.000441 | 0.000662 | 0.006642 | 0.002456 | 0.000168 | 0.000661 | 0.002323 | 0.002887 | 0.000330 | 0.000662 | 0.003659 | 0.002699 |
Nucleolus | 0.002839 | 0.005345 | 0.004681 | 0.000137 | 0.001186 | 0.005140 | 0.000267 | 0.000166 | 0.002164 | 0.005220 | 0.001632 | 0.000153 |
Nucleus | 0.001837 | 0.001530 | 0.001257 | 0.000162 | 0.004267 | 0.001576 | 0.000082 | 0.000186 | 0.002829 | 0.001558 | 0.000445 | 0.000175 |
Peroxisomes | 0.000166 | 0.000235 | 0.000905 | 0.001498 | 0.000042 | 0.000264 | 0.000115 | 0.001524 | 0.000115 | 0.000253 | 0.000360 | 0.001513 |
Vacuole | 0.771993* | 0.854058* | 0.703034* | 0.364125* | 0.924914* | 0.889980* | 0.649947* | 0.380211* | 0.834432* | 0.876013* | 0.666368* | 0.373185* |
Vacuole Periphery | 0.159796* | 0.101343* | 0.166480* | 0.015298 | 0.026880 | 0.070869 | 0.057607 | 0.017106 | 0.105525* | 0.082718 | 0.091285 | 0.016316 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.09 | -1.60 | -6.05 | -6.20 | -5.88 | 0.79 | -4.63 | -7.45 | -7.62 | -6.38 | 1.89 | -5.19 | -9.53 | -9.75 | -8.53 |
Bud Neck | -2.28 | -4.93 | -11.24 | -9.23 | -8.25 | -4.01 | -11.91 | -16.39 | -13.90 | -9.85 | -3.65 | -11.50 | -19.25 | -16.51 | -12.56 |
Bud Site | 3.02 | 2.07 | -2.94 | -5.21 | -4.56 | -3.38 | -13.87 | -11.37 | -10.33 | -5.45 | 3.01 | -4.39 | -9.01 | -11.38 | -6.63 |
Cell Periphery | 5.38 | 11.75 | -33.21* | -36.46* | -39.26* | 8.65 | 2.21 | -23.64 | -30.23* | -26.53* | 10.09 | 5.94 | -39.61 | -46.42* | -44.28* |
Cytoplasm | 2.36 | 2.18 | 1.76 | -1.50 | -0.98 | 0.93 | 2.08 | -1.96 | -2.80 | -3.65 | 2.48 | 2.88 | 0.48 | -3.14 | -3.90 |
Cytoplasmic Foci | -0.86 | -7.03 | 1.76 | 1.44 | 7.50 | 0.79 | -0.89 | -1.61 | -4.84 | -1.17 | 0.31 | -5.01 | -1.76 | -2.55 | 4.12 |
Eisosomes | -8.38 | 8.27 | -10.21 | -5.36 | -10.90 | -4.69 | 6.97 | -2.61 | -0.07 | -5.11 | -9.92 | 8.58 | -8.70 | -3.35 | -10.65 |
Endoplasmic Reticulum | 5.83 | 6.22 | -19.87 | -20.17 | -20.22 | -7.77 | -15.86 | -20.66 | -20.31 | -12.91 | 1.98 | -14.31 | -28.38 | -28.51 | -21.95 |
Endosome | 3.76 | -12.15 | -10.77 | -13.58 | 4.86 | -4.94 | -34.41* | -24.23 | -22.96 | 12.84 | 1.11 | -34.40* | -25.10 | -25.78 | 15.63 |
Golgi | 3.61 | -9.20 | -6.64 | -10.43 | 5.09 | -2.33 | -19.18 | -16.66 | -16.31 | 2.16 | 3.62 | -19.38 | -16.17 | -19.27 | 5.38 |
Lipid Particles | -0.46 | -5.12 | -26.24 | -26.23* | -24.97* | -6.75 | -15.21 | -31.54* | -31.35* | -24.73* | -4.48 | -15.71 | -40.98 | -40.85* | -33.85* |
Mitochondria | -0.85 | -1.87 | 0.78 | 1.56 | 2.57 | -4.55 | -2.52 | -2.69 | 2.65 | -1.21 | -4.17 | -2.04 | -1.38 | 3.09 | 0.15 |
Mitotic Spindle | -0.92 | -2.11 | -2.98 | -1.87 | 0.43 | -2.29 | -1.46 | -2.76 | -0.57 | -0.31 | -2.41 | -1.96 | -3.66 | -1.08 | -0.09 |
None | 2.98 | 3.54 | 3.54 | 4.12 | 0.12 | 1.29 | 2.03 | 1.76 | 3.32 | -2.13 | 3.30 | 4.16 | 3.95 | 5.27 | -2.29 |
Nuclear Periphery | -3.99 | -4.95 | -17.44 | -14.27 | -1.09 | -5.96 | -15.99 | -18.09 | -8.23 | -10.86 | -6.86 | -6.76 | -23.32 | -16.14 | -5.51 |
Nuclear Periphery Foci | -1.51 | -5.00 | -4.36 | -3.72 | 3.16 | -2.76 | -9.61 | -5.92 | -4.93 | -1.16 | -2.95 | -8.09 | -7.28 | -6.18 | 1.88 |
Nucleolus | -3.14 | -1.83 | 5.80 | 7.85 | 4.99 | -5.23 | 3.51 | 3.88 | 7.11 | 7.53 | -5.27 | 1.28 | 6.80 | 10.16 | 5.20 |
Nucleus | 0.48 | 0.97 | 3.59 | 7.26 | 2.99 | 1.66 | 2.54 | 2.47 | 7.93 | -3.82 | 1.72 | 3.22 | 3.59 | 10.68 | 2.25 |
Peroxisomes | -0.96 | -4.85 | -5.13 | -4.70 | -1.93 | -2.83 | -3.11 | -6.81 | -5.46 | -6.48 | -2.44 | -4.78 | -8.35 | -7.18 | -6.63 |
Vacuole | -9.84 | 7.19 | 41.38* | 53.45* | 32.57* | 5.94 | 37.68* | 64.95* | 65.53* | 30.10 | -7.68 | 26.37 | 67.52* | 85.12* | 43.03* |
Vacuole Periphery | 8.04 | -0.80 | 24.65* | 18.01 | 27.34* | -11.03 | -9.16 | 3.21 | 18.47 | 20.82* | 5.03 | 3.28 | 22.80 | 25.63 | 31.73* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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