Standard name | |
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Human Ortholog | |
Description | Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36 (H3K36), which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain(CTD) of Rpo21p; H3K36me3 (trimethylation) requires Spt6p, proline 38 on H3, CTD of Rpo21p, Ctk1p, and C-terminal SRI domain of Ste2p; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 1 | 0 | 0 | 2 | 2 | 6 | – | 10 | 1 | 5 | 8 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 1 | 2 | 1 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 0 | 5 | – | 4 | 6 | 3 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 |
Bud Site | 0 | 0 | 1 | 1 | 2 | 6 | 14 | – | 15 | 10 | 8 | 15 | 2 | 2 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 6 | 2 | 16 | 11 | 13 | 19 | – | 15 | 15 | 19 | 6 | 29 | 59 | 86 | 63 | 33 | 33 | 0 | 0 | 0 | 0 | 0 | 2 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 10 | 6 | 7 | 0 | 0 | 0 | 1 | 1 | 2 |
Endosome | 0 | 1 | 3 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 7 | 2 | 3 | 0 | 1 | 2 | 2 | 1 | 4 |
Golgi | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 2 |
Mitochondria | 5 | 12 | 5 | 6 | 8 | 23 | 46 | – | 125 | 89 | 135 | 99 | 16 | 11 | 17 | 13 | 3 | 23 | 0 | 2 | 2 | 1 | 3 | 2 |
Nucleus | 76 | 322 | 341 | 289 | 390 | 373 | 480 | – | 427 | 370 | 348 | 411 | 540 | 567 | 670 | 505 | 292 | 339 | 84 | 328 | 337 | 190 | 332 | 394 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 2 | 3 | 5 | – | 3 | 5 | 1 | 9 | 4 | 6 | 8 | 3 | 2 | 3 | 0 | 1 | 0 | 0 | 1 | 2 |
Nucleolus | 37 | 76 | 18 | 20 | 50 | 66 | 115 | – | 196 | 152 | 208 | 169 | 118 | 112 | 127 | 41 | 35 | 25 | 10 | 26 | 36 | 69 | 116 | 114 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 2 | 3 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 1 | 2 | 8 | 2 | 9 | 7 | 8 | – | 9 | 3 | 3 | 15 | 3 | 4 | 7 | 6 | 7 | 10 | 1 | 3 | 2 | 0 | 2 | 6 |
Vac/Vac Membrane | 0 | 2 | 5 | 2 | 3 | 2 | 2 | – | 2 | 8 | 9 | 1 | 1 | 2 | 10 | 15 | 8 | 13 | 2 | 12 | 11 | 24 | 28 | 36 |
Unique Cell Count | 86 | 361 | 362 | 316 | 443 | 434 | 567 | 576 | 521 | 556 | 591 | 602 | 685 | 820 | 623 | 353 | 406 | 101 | 382 | 400 | 296 | 503 | 587 | |
Labelled Cell Count | 123 | 423 | 384 | 337 | 479 | 498 | 700 | 806 | 659 | 739 | 744 | 716 | 767 | 936 | 672 | 388 | 459 | 101 | 382 | 400 | 296 | 503 | 587 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.9 | 7.7 | 7.6 | 7.1 | 7.1 | 6.2 | 6.1 | – | 5.5 | 5.6 | 5.4 | 5.5 | 7.3 | 7.0 | 6.8 | 9.5 | 9.9 | 9.1 | 6.8 | 6.8 | 7.2 |
Std Deviation (1e-4) | 1.3 | 1.4 | 1.1 | 1.3 | 1.1 | 1.3 | 1.4 | – | 1.6 | 1.3 | 1.3 | 1.2 | 1.9 | 1.8 | 1.9 | 1.8 | 1.9 | 2.0 | 1.4 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.11 | -0.31 | -0.33 | – | -0.47 | -0.45 | -0.51 | -0.46 | -0.07 | -0.13 | -0.16 | 0.32 | 0.38 | 0.25 | -0.17 | -0.16 | -0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 2.6 | – | 2.7 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.6 | 2.2 | 2.5 | 2.8 | – | 2.3 | 2.5 | 2.8 | 0 | 3.6 | 5.3 | 6.0 | 5.8 | 5.4 | 5.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.5 | 0.5 | 3.0 | 4.4 | – | 8.8 | 7.4 | 9.4 | 7.4 | 1.3 | 0.3 | 0.8 | 0.8 | 0 | 3.2 |
Nucleus | -1.4 | -3.0 | -3.8 | -4.4 | – | -7.8 | -8.6 | -10.8 | -9.1 | -2.4 | -5.2 | -5.6 | -5.7 | -4.8 | -4.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.8 | 3.2 | 4.7 | 6.5 | – | 10.3 | 9.0 | 11.1 | 8.9 | 6.3 | 5.3 | 5.1 | 1.0 | 2.5 | 0.7 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | -0.2 | -0.6 | -0.9 | – | -0.7 | 0 | 0 | 0.3 | 0 | 0 | 0 | -1.6 | -0.2 | 0.2 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0.2 | 0.3 | 0 | 0 | 0 | 0 | 1.1 | 0.9 | 1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.325 | 3.4471 | 3.9818 | 3.5175 | 3.7235 | 3.8371 | 6.5465 | 5.8177 | 6.1699 | 5.9323 | 6.0158 | 6.0492 | 4.3851 | 4.6269 | 4.1163 | 4.3402 | 4.6778 | 4.204 |
Actin | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0137 | 0 | 0.0136 | 0 | 0 | 0.0088 |
Bud | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0004 |
Bud Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 |
Bud Site | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0026 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.0015 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0026 | 0 | 0.0013 | 0 | 0 | 0 |
Cytoplasmic Foci | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0053 | 0 | 0.0006 | 0 | 0 | 0.0009 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0013 | 0 | 0.0012 | 0 | 0 | 0.0002 |
Endosome | 0 | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0079 | 0.0001 | 0.0015 | 0 | 0 | 0.0018 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0015 | 0 | 0.0002 | 0 | 0 | 0.0019 |
Lipid Particles | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0038 | 0 | 0.0005 | 0 | 0 | 0.0037 |
Mitochondria | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0011 | 0.0002 | 0.0002 | 0 | 0.0008 | 0.0006 |
None | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0019 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.0008 | 0.008 | 0.0004 | 0.0009 | 0.0004 | 0.0001 | 0.0002 | 0.0023 | 0.0004 | 0.0001 | 0.0018 | 0.0002 | 0.0187 | 0.001 | 0.0098 | 0.0001 | 0.0015 | 0.001 |
Nucleolus | 0.0368 | 0.0494 | 0.0214 | 0.0139 | 0.2877 | 0.1584 | 0.0199 | 0.0247 | 0.0129 | 0.0132 | 0.0885 | 0.0969 | 0.0341 | 0.0202 | 0.0272 | 0.0083 | 0.2021 | 0.0875 |
Nucleus | 0.9581 | 0.9318 | 0.978 | 0.9837 | 0.7062 | 0.8403 | 0.9779 | 0.9703 | 0.9845 | 0.9865 | 0.9029 | 0.902 | 0.8945 | 0.9762 | 0.9383 | 0.9915 | 0.7935 | 0.889 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.001 | 0 | 0.0005 | 0 | 0 | 0.0025 |
Punctate Nuclear | 0.0042 | 0.0062 | 0.0002 | 0.0012 | 0.0053 | 0.0005 | 0.0019 | 0.0021 | 0.0021 | 0.0002 | 0.0065 | 0.0005 | 0.0067 | 0.0002 | 0.0037 | 0.0001 | 0.0013 | 0.001 |
Vacuole | 0 | 0.0011 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0009 | 0.0009 | 0.0004 | 0 | 0.0001 | 0.0001 |
Vacuole Periphery | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0006 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.2789 | 21.0534 | 15.2902 | 22.982 | 20.79 | 11.0179 | 19.249 | 20.1755 | 19.9459 | 21.5522 |
Translational Efficiency | 1.8268 | 1.3242 | 1.5834 | 1.1885 | 1.1458 | 1.8629 | 1.0855 | 1.0252 | 1.0327 | 1.1036 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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