ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.98 0.98 0.92 0.93 – 0.83 0.83 0.77 0.76 0.76 0.98 0.97 0.96 0.85 0.75 0.78 0.94 0.95 0.93 0.86 0.8 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 – 0.05 0.07 0.1 0.14 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.12 0.06 0.12 0.08 0 0 0 0 0.26 0.38 0.27 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 3 2 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 3 0 0 0 – 3 1 0 0 1 0 0 2 0 0 0 0 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 3 1 2 8 – 12 1 9 6 16 0 2 8 8 15 8 0 0 0 0 0 0
Cytoplasm 274 114 297 246 292 309 – 346 199 266 217 286 213 367 321 168 174 161 261 121 275 173 131 190
Endoplasmic Reticulum 2 1 2 0 3 2 – 4 6 3 9 10 2 5 7 1 6 2 0 0 2 1 1 0
Endosome 1 0 0 0 0 0 – 0 0 0 0 0 2 1 0 0 1 1 1 1 0 2 8 1
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 2 1 1 0 1 6 1
Mitochondria 2 0 2 0 6 16 – 19 17 35 40 67 2 0 1 1 1 1 0 0 3 1 0 0
Nucleus 1 0 0 5 4 2 – 5 6 6 12 12 3 5 9 0 2 3 4 0 4 7 4 5
Nuclear Periphery 0 0 0 0 0 0 – 0 1 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 2 0 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 2 14 11 – 49 15 42 22 14 0 2 3 52 89 55 4 1 2 7 5 8
Unique Cell Count 280 114 302 250 317 333 416 240 344 285 378 218 377 335 198 233 206 278 129 296 201 163 212
Labelled Cell Count 282 115 308 254 323 348 442 248 363 306 409 222 382 351 230 289 233 278 129 296 201 163 212


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.7 22.1 14.2 19.9 17.4 14.5 – 13.4 12.3 12.1 11.8 11.3 22.8 24.2 22.3 19.6 20.3 18.4 21.7 23.9 25.4
Std Deviation (1e-4) 3.7 4.0 3.7 4.6 4.2 3.7 – 3.1 3.3 3.0 3.1 3.0 6.0 4.9 5.1 4.9 8.6 5.5 5.4 6.2 5.9
Intensity Change (Log2) – – – 0.48 0.29 0.02 – -0.09 -0.22 -0.23 -0.27 -0.33 0.68 0.77 0.65 0.46 0.51 0.37 0.61 0.75 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.4 – 1.7 0 1.5 0 2.5 0 0 1.3 0 3.5 0
Cytoplasm 0.1 -3.6 -3.3 – -6.6 -6.4 -8.0 -8.1 -8.4 -0.5 -0.9 -1.9 -5.8 -8.4 -7.5
Endoplasmic Reticulum 0 0 0 – 0 0 0 2.2 2.0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 – 3.1 4.0 5.2 6.3 7.3 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 3.6 3.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.3 2.7 – 5.9 4.0 6.0 4.6 3.0 0 0 0 9.2 11.6 9.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.1552 26.0264 22.8839 20.9844 20.5206 23.6404 20.5747 24.936 22.8039 19.2732 20.5022 22.6053 25.0168 29.5186 26.5773 25.3588 27.8843 26.7408
Actin 0.0047 0.0005 0.0092 0.0175 0.0309 0.0037 0.0221 0.0007 0.0116 0.0436 0.0041 0.0054 0.0007 0.0005 0.0005 0.0236 0.0007 0.0015
Bud 0.0005 0.0001 0.0004 0.0006 0.0002 0.0002 0.0008 0.0001 0.0006 0.0007 0.0001 0.0001 0.0001 0 0 0.0001 0 0.0001
Bud Neck 0.0009 0.0002 0.0045 0.0015 0.0011 0.0009 0.0032 0.0002 0.0006 0.0021 0.0005 0.001 0.0003 0.0003 0.0005 0.0006 0.0008 0.0014
Bud Periphery 0.0004 0.0001 0.0005 0.0008 0.0002 0.0004 0.0007 0.0001 0.0004 0.0008 0.0002 0 0 0 0 0.0001 0 0
Bud Site 0.0005 0.0001 0.0026 0.0027 0.0005 0.0003 0.0041 0.0001 0.0012 0.0022 0.0001 0.0001 0 0 0 0.0002 0 0
Cell Periphery 0.0001 0.0001 0.0002 0.0005 0.0001 0.0001 0.0003 0.0001 0.0001 0.0004 0.0001 0 0 0 0 0 0 0
Cytoplasm 0.9101 0.9816 0.9 0.8641 0.869 0.9213 0.8479 0.9845 0.9631 0.8066 0.9343 0.9465 0.9486 0.991 0.9783 0.9415 0.9855 0.9569
Cytoplasmic Foci 0.0039 0.0007 0.0082 0.0051 0.0035 0.003 0.0236 0.0012 0.0017 0.0133 0.0011 0.0037 0.0008 0.0004 0.0004 0.0035 0.0004 0.0015
Eisosomes 0.0001 0 0.0001 0.0004 0.0002 0.0001 0.0003 0 0.0003 0.0003 0.0001 0 0 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0036 0.0004 0.0027 0.0047 0.0044 0.0014 0.0049 0.0014 0.0008 0.0122 0.0014 0.0021 0.0021 0.0007 0.0009 0.0007 0.0004 0.0012
Endosome 0.0082 0.0019 0.0134 0.0254 0.0153 0.007 0.0154 0.0014 0.0005 0.0324 0.0083 0.0025 0.0022 0.0001 0.0001 0.0033 0.0001 0.0015
Golgi 0.001 0.0003 0.0047 0.0039 0.0063 0.0042 0.0071 0.0002 0.0006 0.0263 0.0077 0.0028 0.0001 0 0 0.0032 0 0.0003
Lipid Particles 0.0014 0.0002 0.0049 0.0027 0.0055 0.0038 0.0089 0.0004 0.0003 0.021 0.0151 0.0022 0 0 0 0.0043 0 0.0008
Mitochondria 0.0019 0.0035 0.0075 0.0078 0.0016 0.0085 0.0053 0.0003 0.0029 0.0082 0.0049 0.0007 0.0004 0 0 0.0007 0.0001 0.0001
None 0.0032 0.0007 0.0051 0.0026 0.0037 0.0006 0.0055 0.0015 0.0006 0.0007 0.0004 0.0009 0.0017 0.0003 0.0004 0.001 0.0005 0.0006
Nuclear Periphery 0.016 0.0008 0.0107 0.0096 0.0237 0.0064 0.0078 0.001 0.0013 0.0047 0.0046 0.0036 0.01 0.0011 0.002 0.0021 0.0013 0.0065
Nucleolus 0.001 0.0001 0.0011 0.0014 0.0005 0.0004 0.0004 0 0 0.0001 0.0001 0 0.0001 0 0 0.0001 0 0.0002
Nucleus 0.0331 0.0053 0.0143 0.0281 0.024 0.0287 0.0239 0.0054 0.0109 0.0138 0.0141 0.0237 0.0304 0.0053 0.0163 0.009 0.0096 0.0204
Peroxisomes 0.0004 0.0001 0.003 0.0008 0.0011 0.0019 0.0082 0 0.0018 0.0042 0.0003 0.0033 0 0 0 0.0031 0 0.0002
Punctate Nuclear 0.0057 0.0002 0.0016 0.0019 0.0059 0.0032 0.0057 0.0002 0.0002 0.001 0.0008 0.0009 0.0014 0.0002 0.0003 0.0024 0.0005 0.0062
Vacuole 0.0023 0.0022 0.0036 0.0127 0.0012 0.0026 0.0025 0.0009 0.0003 0.0037 0.0006 0.0003 0.0005 0.0001 0.0001 0.0002 0.0001 0.0003
Vacuole Periphery 0.001 0.0011 0.0017 0.0051 0.001 0.0015 0.0013 0.0003 0.0001 0.0017 0.0013 0.0001 0.0004 0 0 0.0001 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
522 94 588 184 825 1163 1620 1367 1347 1257 2208 1551

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1234.72 1988.65 1590.18 1949.02 1133.83 1642.86 1709.98 1855.06 1172.93 1668.72 1678.08 1866.21
Standard Deviation 214.44 418.55 225.32 316.02 257.50 326.79 302.12 343.79 246.67 346.67 288.61 341.96
Intensity Change Log 2 — 0.687605 0.365006 0.658565 — 0.535005 0.592775 0.710262 — 0.616565 0.478532 0.683543

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000164 0.001236 0.000332 0.000982 0.000232 0.000460 0.000207 0.004930 0.000205 0.000518 0.000241 0.004462
Bud Neck 0.000265 0.001077 0.000661 0.003544 0.000289 0.000329 0.002368 0.006078 0.000280 0.000385 0.001913 0.005777
Bud Site 0.003358 0.002577 0.002440 0.007598 0.001428 0.002025 0.004347 0.025998 0.002176 0.002067 0.003839 0.023816
Cell Periphery 0.000105 0.000519 0.000107 0.000536 0.000202 0.000073 0.000060 0.000080 0.000164 0.000106 0.000072 0.000134
Cytoplasm 0.882811* 0.818111* 0.642313* 0.632680* 0.782989* 0.771630* 0.637790* 0.724809* 0.821672* 0.775106* 0.638995* 0.713879*
Cytoplasmic Foci 0.010197 0.011867 0.004625 0.017697 0.002117 0.003735 0.002649 0.010453 0.005248 0.004343 0.003175 0.011312
Eisosomes 0.000002 0.000009 0.000003 0.000012 0.000004 0.000002 0.000003 0.000008 0.000003 0.000002 0.000003 0.000008
Endoplasmic Reticulum 0.001031 0.000308 0.000336 0.001564 0.000725 0.000298 0.000281 0.000180 0.000843 0.000298 0.000296 0.000344
Endosome 0.000460 0.002472 0.000274 0.005701 0.000227 0.000684 0.000456 0.007091 0.000317 0.000817 0.000407 0.006926
Golgi 0.000947 0.004639 0.000210 0.025799 0.000201 0.001305 0.000263 0.004648 0.000490 0.001555 0.000249 0.007157
Lipid Particles 0.000145 0.001376 0.000264 0.002803 0.000122 0.000211 0.000192 0.000711 0.000131 0.000298 0.000211 0.000959
Mitochondria 0.000485 0.002769 0.000130 0.000662 0.000119 0.000076 0.000218 0.001265 0.000261 0.000277 0.000195 0.001193
Mitotic Spindle 0.002426 0.000165 0.000516 0.001363 0.000611 0.001482 0.004611 0.042595 0.001314 0.001384 0.003521 0.037703
None 0.015729 0.011741 0.012359 0.007096 0.022504 0.026211 0.011353 0.007446 0.019878 0.025129 0.011621 0.007405
Nuclear Periphery 0.000046 0.000062 0.000164 0.001028 0.000070 0.000036 0.000190 0.000173 0.000060 0.000038 0.000183 0.000275
Nuclear Periphery Foci 0.004229 0.009050 0.002179 0.008545 0.003357 0.002958 0.001504 0.001186 0.003695 0.003413 0.001684 0.002059
Nucleolus 0.000091 0.000108 0.000080 0.000135 0.000616 0.000115 0.000524 0.000475 0.000413 0.000114 0.000406 0.000435
Nucleus 0.070328 0.114629 0.322659* 0.183944* 0.175267* 0.180808* 0.318316* 0.131810* 0.134600* 0.175859* 0.319472* 0.137995*
Peroxisomes 0.000043 0.001316 0.000032 0.000101 0.000024 0.000178 0.000154 0.004475 0.000031 0.000263 0.000122 0.003956
Vacuole 0.006517 0.015805 0.010249 0.089801 0.008631 0.006641 0.014275 0.024225 0.007812 0.007327 0.013203 0.032005
Vacuole Periphery 0.000623 0.000164 0.000066 0.008409 0.000266 0.000744 0.000240 0.001363 0.000405 0.000700 0.000194 0.002199

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.72 -3.69 -6.17 0.52 -2.21 -1.36 -1.04 -1.62 -1.01 -1.39 -1.91 -2.50 -1.88 -0.98 -1.41
Bud Neck -3.66 -6.45 -8.81 -4.72 -6.35 -0.65 -4.78 -7.13 -6.89 0.37 -1.99 -5.45 -8.72 -8.01 -0.80
Bud Site 0.17 0.06 -3.12 -4.69 -4.03 -0.98 -3.31 -5.57 -4.85 -3.06 0.05 -2.09 -5.18 -5.38 -3.76
Cell Periphery -3.60 -2.25 -6.92 -0.27 -6.28 5.84 5.91 5.24 -1.82 -1.86 2.66 4.12 0.51 -2.55 -4.61
Cytoplasm 4.73 26.50 17.70 9.52 2.99 1.47 18.98 6.31 6.12 -15.25 7.04 30.69 15.51 9.23 -13.35
Cytoplasmic Foci -1.55 2.97 -4.38 -2.21 -6.30 -2.38 -3.83 -7.90 -3.25 -4.31 0.72 1.32 -4.40 -4.39 -6.12
Eisosomes -1.57 -4.09 -8.41 -1.11 -6.82 2.33 0.46 -0.30 -5.25 -1.63 1.37 -0.68 -3.14 -5.54 -4.02
Endoplasmic Reticulum 1.22 0.92 0.90 -1.06 0.03 2.91 3.33 4.91 2.91 6.20 2.21 2.11 2.97 2.37 5.89
Endosome -0.93 0.22 -1.31 0.54 -1.39 -1.54 -1.52 -2.36 -1.28 -1.97 -1.57 -1.06 -2.39 -1.10 -2.15
Golgi -1.14 1.27 -0.83 0.19 -1.09 -2.06 -0.17 -1.93 0.69 -1.85 -1.76 1.22 -1.32 0.50 -2.04
Lipid Particles -2.07 -5.11 -9.07 -2.12 -7.71 -1.60 -4.20 -8.65 -6.25 -4.24 -2.52 -5.80 -11.77 -7.13 -6.95
Mitochondria -0.78 0.85 0.76 0.93 -0.63 1.50 -3.50 -1.35 -1.73 -0.43 0.12 0.31 -0.09 -0.22 -0.57
Mitotic Spindle 1.48 1.11 -0.02 -3.11 -2.33 -0.69 -3.18 -4.79 -4.48 -2.74 0.03 -2.23 -4.49 -4.56 -3.23
None 0.97 1.66 3.70 0.91 4.84 -0.87 4.52 6.16 9.22 8.33 -1.56 4.74 7.13 9.21 9.49
Nuclear Periphery -2.40 -10.25 -6.12 -5.41 -3.52 1.59 -7.48 -8.33 -17.31 0.49 1.33 -10.82 -12.12 -16.50 -1.31
Nuclear Periphery Foci -2.96 1.31 -1.88 1.90 -2.77 0.52 2.72 3.20 4.27 0.53 0.24 3.48 3.86 3.95 0.18
Nucleolus -0.62 -0.66 -1.73 -0.50 -2.29 1.44 0.47 0.95 -2.74 1.24 1.39 0.26 0.67 -2.75 0.89
Nucleus -4.54 -29.99 -10.72 -4.76 9.22 -0.99 -17.63 4.16 6.98 29.40 -7.23 -30.88 -2.87 5.18 32.03
Peroxisomes -1.00 -0.20 -2.37 0.96 -2.46 -2.14 -2.92 -1.97 -1.65 -1.74 -2.17 -2.84 -1.97 -1.48 -1.78
Vacuole -3.56 -5.92 -11.76 -9.68 -10.68 1.04 -10.96 -20.97 -22.18 -12.19 -0.64 -12.75 -24.31 -24.00 -16.40
Vacuole Periphery 1.29 1.50 -0.55 -1.26 -1.36 -1.24 0.65 -0.01 1.27 -0.78 -0.75 1.53 0.20 0.92 -1.58
ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

YJL169W

YJL169W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yjl169w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available