Standard name
Human Ortholog
Description GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.12 0.11 0.08 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0.05 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.1 0.06 0 0 0 0 0 0.05 0.11 0.1 0.16 0 0 0 0 0 0 0 0 0
Cytoplasm 0.49 0.32 0.37 0.3 0.32 0.51 0.56 0.59 0.63 0.69 0.66 0.17 0.23 0.23 0.53 0.61 0.51 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.44 0.71 0.64 0.49 0.48 0.35 0.23 0.17 0.12 0.06 0.13 0.76 0.73 0.71 0 0 0 0.8 0.85 0.88 0.86 0.89 0.86
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0 0.06 0.12 0.16 0.09 0.16 0.16 0.26 0.22 0.19 0.06 0.05 0 0.49 0.39 0.51 0.15 0.05 0 0.07 0.05 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 8 25 37 63 59 40 30 29 31 40 25 9 13 3 2 2 0 4 8 4 5 3
Bud 0 2 1 12 11 40 43 36 25 47 31 2 1 1 0 1 0 1 1 2 1 1 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 1 2 1
Bud Site 0 0 0 0 0 1 1 1 2 2 0 0 0 0 0 0 0
Cell Periphery 0 13 9 32 35 31 37 17 27 28 47 68 51 113 0 5 4 0 0 0 0 0 1
Cytoplasm 237 160 208 95 179 356 468 392 495 490 632 99 118 165 126 103 78 11 24 15 12 10 7
Endoplasmic Reticulum 215 352 358 155 269 248 191 112 96 40 123 454 375 500 2 5 3 432 450 534 396 492 498
Endosome 4 0 9 4 7 1 13 12 14 8 5 1 2 1 1 2 2 2 3 3 2 0 2
Golgi 1 0 0 9 6 1 0 0 1 0 0 5 1 1 4 3 5 1 3 1 3 3 4
Mitochondria 1 1 2 5 3 5 8 10 10 16 25 0 0 0 1 0 1 2 1 4 4 3 2
Nucleus 1 1 1 0 2 3 6 8 13 10 15 2 0 2 4 3 2 0 0 0 0 0 0
Nuclear Periphery 2 2 0 0 0 0 12 3 2 1 8 2 3 4 0 0 0 2 2 2 2 2 1
Nucleolus 0 0 0 2 2 1 4 6 7 0 4 0 0 0 0 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 82 13 35 39 90 62 129 108 203 152 187 33 28 16 115 66 78 81 26 26 31 25 44
Unique Cell Count 486 497 558 314 557 704 830 661 787 706 960 595 511 709 237 170 152 541 527 611 463 555 578
Labelled Cell Count 546 552 648 390 667 808 952 735 924 825 1117 691 588 818 256 190 177 541 527 611 463 555 578


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.9 12.5 10.4 13.1 13.1 12.3 10.9 10.3 9.5 9.8 9.5 15.0 14.2 13.1 18.5 19.2 19.3 14.8 16.0 16.7
Std Deviation (1e-4) 2.3 2.0 2.3 2.7 2.1 2.1 1.9 1.9 1.8 3.1 2.1 3.1 2.8 2.4 4.6 5.6 6.7 2.8 3.4 3.8
Intensity Change (Log2) 0.33 0.32 0.24 0.06 -0.01 -0.14 -0.09 -0.13 0.52 0.44 0.33 0.83 0.88 0.89 0.5 0.62 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 4.0 4.2 2.8 0.3 0 -0.7 -0.1 -0.3 -0.2 -2.5 -2.7 -2.2 -2.0 -1.8
Bud 0 2.9 5.5 5.2 5.3 3.9 6.0 4.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 5.7 4.0 2.8 2.9 1.2 2.0 2.5 3.3 6.7 5.9 8.6 0 0 0
Cytoplasm -2.1 -1.8 4.7 7.0 7.7 9.3 11.4 10.8 -7.9 -5.0 -5.4 4.2 5.4 3.1
Endoplasmic Reticulum -4.3 -5.3 -10.2 -15.4 -16.9 -19.9 -22.2 -20.7 4.5 3.2 2.4 -16.4 -14.0 -13.6
Endosome 0 -0.5 0 -0.1 0.3 0.2 -0.7 -2.1 0 -2.0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 2.0 0 2.8 3.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 2.6 0 2.5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.1 5.2 1.7 5.2 5.4 9.2 7.6 7.0 -0.5 -0.5 -3.6 13.9 10.7 13.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.0828 10.793 10.4452 10.3617 10.0345 10.2516 16.0233 16.2417 15.7792 14.532 14.4358 15.7058 12.8048 12.757 11.9005 11.4858 11.7317 12.5763
Actin 0.0007 0.0013 0.002 0.0025 0.0001 0.0004 0.0037 0.0007 0.0004 0.0001 0.0006 0.0003 0.0063 0.0005 0.0022 0.0017 0.0026 0.0036
Bud 0.0001 0.0017 0.0001 0.0005 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0 0 0 0 0.0001 0.0001 0.0002
Bud Neck 0.0004 0.0005 0.0003 0.0004 0.0026 0.0008 0.0002 0.0002 0.0002 0.0001 0.0012 0.0002 0.0002 0.0001 0.0001 0.0003 0.0016 0.0004
Bud Periphery 0.0001 0.0009 0.0001 0.0009 0.0002 0.0001 0.0002 0.0003 0.0001 0 0.0003 0 0.0001 0 0 0.0002 0.0003 0.0007
Bud Site 0.0003 0.0023 0.0005 0.0008 0.0001 0.0002 0.0004 0.0003 0.0003 0 0.0003 0 0.0003 0.0002 0.0001 0.0005 0.0017 0.0001
Cell Periphery 0.0006 0.0044 0.0012 0.0008 0.0015 0.0006 0.0011 0.0049 0.0025 0.0005 0.0033 0.0004 0.0011 0.0014 0.001 0.0003 0.0015 0.0009
Cytoplasm 0.048 0.1134 0.0727 0.0382 0.0621 0.0549 0.0107 0.0389 0.0164 0.0322 0.033 0.016 0.0198 0.0414 0.0191 0.0085 0.0177 0.0171
Cytoplasmic Foci 0.0214 0.0022 0.0052 0.0104 0.0012 0.0074 0.0031 0.0006 0.0007 0.0003 0.0008 0.0005 0.0071 0.0008 0.0018 0.0029 0.0058 0.003
Eisosomes 0 0 0 0 0 0 0.0003 0 0 0 0 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.776 0.8022 0.8642 0.8625 0.8668 0.8713 0.9096 0.9272 0.9593 0.9554 0.9348 0.9549 0.9013 0.9382 0.9406 0.9396 0.92 0.9435
Endosome 0.0569 0.0183 0.0129 0.0202 0.0082 0.0105 0.013 0.0022 0.0023 0.0008 0.0023 0.0037 0.0099 0.0019 0.0069 0.023 0.0119 0.0046
Golgi 0.0052 0.0011 0.0046 0.0015 0.0004 0.0048 0.0028 0.0004 0.0003 0.0001 0.0006 0.0006 0.0026 0.0002 0.003 0.0036 0.0021 0.0034
Lipid Particles 0.0182 0.007 0.0086 0.002 0.0048 0.001 0.0066 0.0068 0.0036 0.0029 0.0075 0.0021 0.0113 0.0038 0.0048 0.0025 0.0063 0.0034
Mitochondria 0.0013 0.0004 0.0031 0.0021 0.0001 0.001 0.004 0.0001 0 0 0.0001 0.0001 0.0062 0 0.0004 0.0015 0.0018 0.0008
None 0.0021 0.0004 0.0003 0.0054 0.0003 0.0009 0.0007 0.0002 0.0001 0.0001 0.002 0.0002 0.0025 0.0001 0.0011 0.0002 0.0013 0.0004
Nuclear Periphery 0.0358 0.0167 0.0101 0.0123 0.0165 0.0239 0.025 0.0089 0.0037 0.0036 0.0055 0.016 0.0147 0.0048 0.0071 0.0089 0.0093 0.0071
Nucleolus 0.0002 0.0002 0.0001 0.0003 0.0001 0.0001 0.0002 0 0 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0011 0.0001
Nucleus 0.0069 0.0029 0.0011 0.0049 0.0047 0.0045 0.0027 0.0007 0.0003 0.0004 0.0011 0.0013 0.0013 0.0004 0.0005 0.0013 0.0021 0.0009
Peroxisomes 0.0055 0 0.0034 0.0015 0 0.0004 0.0034 0 0 0 0 0 0.0034 0 0.0024 0.0003 0.0012 0.0022
Punctate Nuclear 0.0056 0.0004 0.0003 0.0099 0.0003 0.0008 0.0045 0.0001 0.0001 0 0.0005 0.0003 0.0038 0.0001 0.0021 0.0007 0.0035 0.0016
Vacuole 0.0123 0.0231 0.0086 0.0199 0.0291 0.0153 0.0061 0.007 0.0093 0.0033 0.0057 0.003 0.0066 0.0058 0.0063 0.0032 0.0074 0.0055
Vacuole Periphery 0.0025 0.0007 0.0007 0.0031 0.0009 0.001 0.0015 0.0003 0.0003 0.0002 0.0002 0.0003 0.0013 0.0001 0.0003 0.0006 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 92.182 90.2729 71.2883 79.7377 64.864 81.4827 116.6057 91.5588 92.3407 100.8246
Translational Efficiency 1.057 1.2501 1.4034 1.3303 1.4075 1.6335 1.271 1.2816 1.1635 1.0989

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1543 1902 272 1552 1802 1653 1912 1367 3345 3555 2184 2919

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 922.72 1103.48 1525.06 1776.57 1072.49 1139.52 1590.48 1616.38 1003.40 1120.24 1582.33 1701.55
Standard Deviation 124.27 191.39 223.28 406.49 215.09 190.59 223.68 472.55 193.96 191.86 224.67 445.89
Intensity Change Log 2 0.258096 0.724901 0.945130 0.087462 0.568498 0.591802 0.168889 0.642942 0.766000

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000254 0.000432 0.000778 0.001842 0.000612 0.000310 0.000178 0.002085 0.000446 0.000375 0.000253 0.001956
Bud Neck 0.000747 0.001434 0.002220 0.004060 0.000764 0.000254 0.000214 0.004027 0.000756 0.000885 0.000464 0.004045
Bud Site 0.003788 0.004043 0.003475 0.016727 0.006261 0.001964 0.001098 0.026938 0.005120 0.003076 0.001394 0.021509
Cell Periphery 0.001171 0.002050 0.001442 0.025220 0.001798 0.002010 0.003010 0.021301 0.001509 0.002031 0.002815 0.023385
Cytoplasm 0.069345 0.067865 0.023318 0.036364 0.044018 0.024753 0.006742 0.059634 0.055701 0.047819 0.008806 0.047262
Cytoplasmic Foci 0.008688 0.012353 0.006995 0.003848 0.019972 0.006132 0.000202 0.011724 0.014767 0.009460 0.001048 0.007536
Eisosomes 0.000204 0.000168 0.000043 0.000173 0.000122 0.000065 0.000020 0.000203 0.000160 0.000120 0.000023 0.000187
Endoplasmic Reticulum 0.862862* 0.831782* 0.803939* 0.797876* 0.856089* 0.934007* 0.976118* 0.718035* 0.859213* 0.879315* 0.954675* 0.760485*
Endosome 0.004939 0.009918 0.019366 0.011252 0.012203 0.006378 0.002025 0.018037 0.008852 0.008272 0.004185 0.014429
Golgi 0.005192 0.004760 0.001771 0.006956 0.006907 0.003155 0.000359 0.012546 0.006116 0.004014 0.000535 0.009574
Lipid Particles 0.009541 0.008028 0.014674 0.044724 0.006741 0.003440 0.002604 0.056333 0.008032 0.005895 0.004107 0.050161
Mitochondria 0.002858 0.001891 0.000042 0.002216 0.002700 0.001420 0.000214 0.001826 0.002773 0.001672 0.000193 0.002034
Mitotic Spindle 0.000267 0.000120 0.000418 0.000932 0.000111 0.000471 0.000018 0.001584 0.000183 0.000284 0.000067 0.001237
None 0.002632 0.003676 0.000083 0.002690 0.003583 0.001160 0.001113 0.001419 0.003144 0.002506 0.000985 0.002094
Nuclear Periphery 0.002196 0.001263 0.010040 0.000796 0.002587 0.000357 0.000217 0.000844 0.002407 0.000842 0.001440 0.000819
Nuclear Periphery Foci 0.003857 0.008182 0.060641 0.008906 0.007872 0.002105 0.001204 0.017630 0.006020 0.005357 0.008607 0.012992
Nucleolus 0.000172 0.000249 0.000005 0.000088 0.000202 0.000040 0.000003 0.000096 0.000188 0.000152 0.000003 0.000092
Nucleus 0.004759 0.003042 0.000836 0.002031 0.001916 0.000631 0.000129 0.003276 0.003228 0.001921 0.000217 0.002614
Peroxisomes 0.000965 0.000828 0.000150 0.000767 0.000555 0.000589 0.000091 0.000657 0.000744 0.000717 0.000098 0.000716
Vacuole 0.014167 0.035136 0.049147 0.029089 0.020704 0.009160 0.004291 0.038053 0.017689 0.023058 0.009877 0.033287
Vacuole Periphery 0.001397 0.002779 0.000615 0.003442 0.004283 0.001599 0.000150 0.003751 0.002952 0.002230 0.000208 0.003587

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.92 -1.92 -3.65 -3.26 -2.05 1.50 2.62 -3.66 -4.42 -4.86 0.55 1.64 -5.03 -5.29 -5.65
Bud Neck -1.76 -3.39 -4.78 -3.75 -2.69 3.23 2.73 -7.64 -9.14 -8.95 -0.57 1.48 -7.60 -7.04 -8.07
Bud Site -0.23 0.15 -6.95 -7.31 -6.88 3.96 4.67 -7.96 -9.92 -10.20 2.59 4.42 -10.46 -12.26 -13.12
Cell Periphery -2.13 -0.93 -13.68 -12.90 -13.39 -0.47 -2.52 -11.57 -11.53 -10.84 -1.67 -3.66 -17.84 -17.28 -16.46
Cytoplasm 0.29 7.90 7.40 7.50 -2.70 6.40 14.39 -3.92 -8.62 -13.53 2.87 21.43 1.84 -0.67 -16.32
Cytoplasmic Foci -2.46 0.60 4.08 6.47 1.28 6.92 11.45 3.67 -3.54 -9.00 4.07 12.72 5.59 1.63 -9.03
Eisosomes 0.83 4.65 0.75 -0.27 -6.71 2.65 5.38 -2.19 -4.10 -5.69 1.66 7.55 -1.20 -2.96 -9.55
Endoplasmic Reticulum 3.78 3.80 7.92 4.57 0.64 -10.47 -18.08 12.76 21.24 26.51* -3.41 -17.87 14.56 17.78 29.62
Endosome -7.62 -7.11 -8.85 -1.88 3.90 5.14 11.76 -5.02 -10.23 -17.81 0.84 7.72 -8.02 -9.15 -16.58
Golgi 0.49 4.22 -1.45 -1.96 -4.75 4.09 8.32 -3.56 -6.43 -8.81 3.34 10.51 -3.50 -6.15 -10.76
Lipid Particles 1.37 -2.14 -11.20 -11.83 -7.19 4.19 4.53 -13.56 -14.52 -14.63 3.17 4.40 -17.63 -18.63 -18.87
Mitochondria 1.48 4.68 0.74 -0.62 -4.18 2.08 4.72 1.33 -0.98 -4.95 2.48 6.48 1.36 -1.07 -5.81
Mitotic Spindle 1.36 -1.00 -2.25 -2.61 -1.77 -1.15 2.45 -4.95 -2.76 -5.29 -0.63 1.96 -4.44 -3.77 -4.88
None -1.02 4.39 -0.12 0.91 -4.34 2.26 2.29 2.05 -0.71 -0.81 0.82 3.15 1.45 0.64 -2.48
Nuclear Periphery 2.44 -5.05 4.27 2.00 6.06 4.23 4.54 3.25 -4.18 -6.96 4.63 2.58 4.88 -0.04 2.86
Nuclear Periphery Foci -4.15 -9.05 -5.50 -0.92 8.14 5.66 6.55 -5.44 -9.51 -10.03 0.81 -2.51 -7.03 -7.90 -3.79
Nucleolus -0.61 2.17 1.02 1.52 -2.72 3.40 4.30 1.98 -2.61 -4.76 0.46 4.21 1.95 1.02 -4.77
Nucleus 3.23 8.61 4.15 1.09 -4.38 4.30 7.16 -2.37 -4.10 -4.86 4.27 12.73 0.21 -2.72 -7.34
Peroxisomes 0.50 4.68 1.01 0.31 -3.59 -0.30 7.48 -0.68 -0.38 -4.22 0.17 7.98 0.32 0.10 -5.96
Vacuole -7.50 -5.98 -7.09 1.04 3.01 5.38 8.03 -6.39 -11.05 -13.12 -2.99 4.38 -9.17 -5.95 -12.87
Vacuole Periphery -3.30 2.23 -3.48 -1.03 -4.62 4.63 8.97 0.70 -3.45 -6.86 1.95 10.08 -1.42 -3.01 -8.80
Standard name
Human Ortholog
Description GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Toh1

Toh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Toh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available