Standard name
Human Ortholog
Description ER-membrane protein; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5 of TOM (Translocase of the Mitochondrial Outer Membrane) complex; suppressor of pma1-7, deletion of SOP4 slows down export of wild-type Pma1p and Pma1-7 from the ER
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0.11 0.15 0.07 0.1 0.06 0.05 0.09 0.08 0.09 0.09 0.05 0.09 0 0.11 0.19 0.19 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.08 0.09 0.06 0.05 0.05 0 0.08 0.07 0.1 0.07 0.14 0.18 0.22 0.27 0.29 0.28 0 0 0 0 0 0
Cytoplasm 0.05 0 0.06 0.07 0.08 0.1 0.11 0.08 0.1 0.1 0.11 0.08 0 0.05 0.06 0.18 0.15 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0.91 0.94 0.88 0.77 0.76 0.63 0.61 0.61 0.54 0.39 0.58 0.53 0.91 0.83 0.71 0 0 0 0.92 0.95 0.92 0.86 0.86 0.87
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.08 0.15 0.05 0 0 0 0 0 0 0 0.05 0.06 0.06 0.37 0.3 0.3 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.12 0.15 0.29 0.25 0.21 0.38 0.39 0.41 0.44 0 0 0 0.06 0.07 0.13 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.05 0 0 0 0 0.07 0.05 0.06 0.07 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0 0 0.09 0.13 0.11 0.18 0.13 0.19 0.05 0.08 0 0.06 0.13 0.12 0.11 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 15 54 50 27 57 29 37 49 43 47 47 31 54 31 21 36 35 3 2 12 13 11 15
Bud 2 1 1 2 3 11 4 12 9 15 6 16 3 0 0 3 3 3 0 0 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 8 42 32 21 32 24 25 46 41 56 41 89 115 158 51 56 52 0 0 5 5 6 17
Cytoplasm 14 9 29 25 30 59 54 55 55 54 57 42 20 34 45 33 29 30 5 3 2 3 4 4
Endoplasmic Reticulum 234 239 445 261 275 368 302 426 306 220 312 289 570 524 505 1 7 3 254 252 485 317 390 521
Endosome 0 0 0 2 2 1 0 0 1 3 0 1 0 0 0 1 0 0 0 0 1 1 1 2
Golgi 4 4 40 51 17 3 0 3 2 1 0 2 30 38 41 69 58 57 2 0 3 7 9 7
Mitochondria 2 1 60 40 54 171 122 145 213 221 221 239 1 0 1 12 13 25 2 1 4 12 10 8
Nucleus 2 1 1 0 0 2 0 2 2 5 0 3 0 1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 8 2 24 6 10 21 18 46 28 33 38 30 13 8 14 6 9 8 1 0 3 0 2 2
Nucleolus 0 0 0 0 0 0 1 2 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 23 11 13 11 34 76 56 126 71 109 28 45 8 35 91 23 22 22 3 3 1 4 8 9
Unique Cell Count 258 255 506 337 363 585 492 703 564 560 539 549 623 632 711 188 192 188 275 267 525 370 451 597
Labelled Cell Count 302 291 709 480 474 802 610 881 782 747 765 757 765 809 886 220 233 235 275 267 525 370 451 597


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.2 21.1 13.8 13.4 11.5 10.3 9.5 9.7 9.4 8.5 8.9 8.8 19.6 18.9 19.0 21.1 21.8 22.2 20.8 20.8 21.7
Std Deviation (1e-4) 3.9 3.9 2.5 2.4 1.9 1.7 1.7 1.8 1.8 2.1 1.8 1.8 3.7 3.6 3.3 4.8 4.8 5.0 4.1 5.8 4.5
Intensity Change (Log2) – – – -0.04 -0.27 -0.42 -0.54 -0.51 -0.55 -0.7 -0.63 -0.64 0.51 0.46 0.46 0.62 0.66 0.69 0.59 0.6 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.8 -1.6 -0.5 -2.7 -3.5 -1.1 -1.7 -1.1 -1.2 -3.6 -1.2 -4.3 0.2 2.8 2.8
Bud 0 0 2.7 0 2.5 0 3.3 0 3.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.6 -1.4 -1.9 -2.2 -3.6 -0.1 -0.6 1.2 -0.5 3.1 4.8 6.5 6.5 7.1 6.6
Cytoplasm 1.0 1.5 2.6 3.0 1.4 2.4 2.4 2.8 1.2 -2.1 -0.3 0.4 4.9 4.0 4.3
Endoplasmic Reticulum -4.0 -4.7 -9.5 -9.7 -10.5 -12.0 -16.4 -10.9 -12.5 2.0 -2.4 -7.0 -21.4 -20.8 -21.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.3 -1.9 -6.3 -6.4 -6.9 -6.3 -6.6 -6.7 -6.3 -2.1 -1.3 -1.5 9.3 7.6 7.6
Mitochondria 0 1.3 7.0 5.3 4.0 9.7 10.2 10.6 11.4 -8.6 -8.9 -9.2 -2.1 -2.0 0.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.3 -1.5 -1.0 -0.9 1.3 0.2 0.8 1.6 0.5 -2.5 -3.5 -2.7 -0.9 0 -0.3
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.6 4.4 6.3 5.5 8.3 6.1 8.7 2.2 4.0 -1.6 2.5 6.3 5.1 4.8 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 108.7753 82.5478 89.2855 108.06 89.2864 68.8268 88.4152 84.9762 76.7524 102.5137
Translational Efficiency 1.0511 0.9662 0.966 0.7889 1.081 1.65 1.0274 1.1494 1.1625 0.9644

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
871 2146 206 5 1209 1313 1502 3 2080 3459 1708 8

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1016.77 1604.60 1963.98 2494.91 1230.95 1237.31 1780.47 429.17 1141.26 1465.18 1802.60 1720.26
Standard Deviation 197.00 290.25 293.59 462.68 291.20 256.37 288.79 11.60 276.96 330.13 295.48 1064.89
Intensity Change Log 2 — 0.658220 0.949787 1.294994 — 0.007435 0.532486 -1.520151 — 0.338399 0.736291 0.379520

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007092 0.000439 0.000988 0.040500 0.001651 0.001645 0.002610 0.000030 0.003929 0.000897 0.002414 0.025324
Bud Neck 0.000336 0.000091 0.002108 0.004760 0.000916 0.002275 0.001524 0.000001 0.000673 0.000920 0.001594 0.002976
Bud Site 0.006618 0.001371 0.001772 0.081337 0.009907 0.007790 0.010461 0.000007 0.008530 0.003808 0.009413 0.050838
Cell Periphery 0.004730 0.002826 0.005300 0.020482 0.003329 0.006690 0.008877 0.000117 0.003916 0.004293 0.008446 0.012845
Cytoplasm 0.005742 0.001848 0.000337 0.000404 0.005198 0.004785 0.011082 0.000024 0.005426 0.002963 0.009786 0.000261
Cytoplasmic Foci 0.005982 0.002043 0.000061 0.000059 0.017045 0.005195 0.007229 0.000004 0.012412 0.003239 0.006365 0.000038
Eisosomes 0.000436 0.000086 0.000071 0.000968 0.000071 0.000173 0.000161 0.000116 0.000224 0.000119 0.000150 0.000648
Endoplasmic Reticulum 0.926866* 0.974561* 0.953976* 0.821446* 0.850468* 0.942951* 0.868731* 0.000002 0.882459* 0.962562* 0.879012* 0.513404*
Endosome 0.011023 0.005288 0.007110 0.004848 0.023813 0.006414 0.017975 0.000000 0.018457 0.005716 0.016665 0.003030
Golgi 0.007508 0.002692 0.002044 0.002356 0.014917 0.003431 0.013925 0.000002 0.011814 0.002972 0.012492 0.001473
Lipid Particles 0.005032 0.002886 0.002611 0.011310 0.006338 0.005697 0.026270 0.000019 0.005791 0.003953 0.023416 0.007076
Mitochondria 0.005431 0.000525 0.000123 0.000096 0.002871 0.001964 0.001434 0.000012 0.003943 0.001071 0.001276 0.000064
Mitotic Spindle 0.000310 0.000251 0.000037 0.000047 0.000274 0.000118 0.001641 0.000110 0.000289 0.000201 0.001447 0.000070
None 0.000784 0.001915 0.000010 0.000176 0.004628 0.001846 0.001345 0.999435* 0.003018 0.001889 0.001184 0.374898*
Nuclear Periphery 0.001268 0.000163 0.003713 0.000723 0.000981 0.000273 0.000828 0.000000 0.001101 0.000205 0.001176 0.000452
Nuclear Periphery Foci 0.004457 0.000870 0.007497 0.001456 0.010534 0.001545 0.010567 0.000000 0.007990 0.001126 0.010197 0.000910
Nucleolus 0.000061 0.000003 0.000004 0.000002 0.000106 0.000080 0.000016 0.000001 0.000087 0.000032 0.000015 0.000001
Nucleus 0.001013 0.000062 0.000063 0.000405 0.000587 0.000829 0.000273 0.000003 0.000765 0.000353 0.000248 0.000254
Peroxisomes 0.001510 0.000603 0.000006 0.000013 0.000493 0.001381 0.000789 0.000116 0.000919 0.000898 0.000695 0.000051
Vacuole 0.002218 0.001103 0.010604 0.008321 0.036528 0.003307 0.012069 0.000000 0.022160 0.001939 0.011893 0.005201
Vacuole Periphery 0.001583 0.000374 0.001565 0.000292 0.009345 0.001610 0.002192 0.000000 0.006095 0.000843 0.002116 0.000183

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Bud Neck NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Bud Site NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cell Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cytoplasm NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cytoplasmic Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Eisosomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Endoplasmic Reticulum NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Endosome NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Golgi NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Lipid Particles NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Mitochondria NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Mitotic Spindle NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
None NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nuclear Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nuclear Periphery Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nucleolus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nucleus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Peroxisomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Vacuole NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Vacuole Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Standard name
Human Ortholog
Description ER-membrane protein; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5 of TOM (Translocase of the Mitochondrial Outer Membrane) complex; suppressor of pma1-7, deletion of SOP4 slows down export of wild-type Pma1p and Pma1-7 from the ER
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Sop4

Sop4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sop4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available