Standard name | |
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Human Ortholog | |
Description | Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 1 | 1 | 5 | 3 | 6 | 6 | 5 | 7 | 0 | 0 | 0 | 1 | 0 | 0 | 7 | 9 | 4 | 2 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 5 | 5 | 5 | 7 | 16 | 9 | 17 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 18 | 10 | 15 | 14 | 34 | 18 | 52 | 48 | 25 | 42 | 38 | 48 | 5 | 11 | 15 | 34 | 17 | 43 | 5 | 2 | 1 | 0 | 2 | 0 |
Endoplasmic Reticulum | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 4 | 4 | 0 | 1 | 1 | 0 | 1 | 0 |
Endosome | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 3 | 2 | 2 | 0 | 2 | 4 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 4 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 37 | 67 | 46 | 119 | 216 | 211 | 191 | 218 | 195 | 314 | 0 | 0 | 1 | 5 | 1 | 2 | 0 | 0 | 3 | 0 | 1 | 0 |
Nucleus | 417 | 245 | 283 | 207 | 278 | 213 | 289 | 344 | 187 | 197 | 181 | 265 | 8 | 33 | 25 | 87 | 76 | 73 | 458 | 286 | 294 | 33 | 78 | 42 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 1 | 0 | 1 | 1 | 2 | 6 | 7 | 3 | 8 | 2 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 3 | 1 | 3 | 6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 2 | 0 | 0 | 1 |
Vac/Vac Membrane | 69 | 64 | 20 | 4 | 48 | 29 | 33 | 41 | 17 | 31 | 19 | 44 | 5 | 9 | 14 | 41 | 38 | 56 | 22 | 19 | 32 | 5 | 16 | 20 |
Unique Cell Count | 483 | 301 | 325 | 241 | 363 | 309 | 468 | 532 | 316 | 376 | 318 | 478 | 16 | 51 | 56 | 157 | 129 | 153 | 518 | 331 | 353 | 45 | 117 | 87 |
Labelled Cell Count | 508 | 320 | 359 | 294 | 408 | 387 | 609 | 662 | 438 | 518 | 450 | 702 | 19 | 54 | 57 | 173 | 142 | 182 | 518 | 331 | 353 | 45 | 117 | 87 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.7 | 9.0 | 5.8 | 5.3 | 5.5 | 5.0 | 4.7 | 4.9 | 4.3 | 4.4 | 4.3 | 4.1 | 8.2 | 8.6 | 7.3 | 12.4 | 14.0 | 13.0 | 7.6 | 9.7 | 10.8 |
Std Deviation (1e-4) | 1.5 | 1.3 | 1.1 | 1.0 | 1.0 | 1.0 | 1.2 | 1.2 | 0.9 | 1.2 | 1.1 | 1.0 | 1.6 | 1.3 | 1.5 | 2.7 | 2.9 | 2.9 | 1.6 | 2.0 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.09 | -0.23 | -0.3 | -0.26 | -0.44 | -0.4 | -0.46 | -0.52 | 0.48 | 0.56 | 0.33 | 1.09 | 1.26 | 1.16 | 0.38 | 0.74 | 0.89 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | 2.4 | 0.7 | 3.2 | 2.4 | 1.7 | 3.2 | 3.4 | 2.8 | 0 | 0 | 0 | 5.8 | 3.2 | 7.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.0 | 0.5 | 7.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.5 | -2.2 | -3.0 | -3.7 | -3.8 |
Nucleus | -0.4 | -3.5 | -5.5 | -7.8 | -7.2 | -8.0 | -9.9 | -8.5 | -9.5 | -4.1 | -4.1 | -7.5 | -7.7 | -6.7 | -9.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.6 | 3.1 | 1.5 | 0.5 | 0.9 | -0.4 | 1.1 | -0.1 | 1.6 | 0 | 0 | 0 | 6.2 | 6.7 | 8.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8345 | 5.5785 | 5.1852 | 4.7313 | 4.2043 | 4.8295 | 5.3564 | 6.407 | 5.9207 | 5.3048 | 5.6584 | 5.7963 | 6.5357 | 7.0424 | 6.491 | 6.3834 | 6.9107 | 6.5224 |
Actin | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0008 | 0.0155 | 0.0006 | 0.0224 | 0.0022 | 0.0276 | 0.0022 | 0.0094 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0007 | 0.0001 | 0.0009 | 0.0003 | 0.0008 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0009 | 0.0045 | 0.0062 | 0.0003 | 0.0019 | 0.0012 | 0.0014 | 0.0011 | 0.0002 | 0.0001 | 0.0022 | 0.0001 | 0.0007 | 0.0006 |
Bud Periphery | 0 | 0.0001 | 0 | 0 | 0 | 0.0008 | 0.001 | 0.0001 | 0.0013 | 0.0005 | 0.0015 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0001 | 0.0097 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0027 | 0.0006 | 0.009 | 0.001 | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0009 | 0 |
Cell Periphery | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0013 | 0.0138 | 0.0018 | 0.0063 | 0.0077 | 0.0033 | 0.0115 | 0.0147 | 0.0148 | 0.0055 | 0.0018 | 0.0021 | 0.02 | 0.0231 | 0.0029 | 0.0019 | 0.0026 | 0.0006 |
Cytoplasmic Foci | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0315 | 0.0022 | 0.016 | 0.0191 | 0.0107 | 0.0007 | 0.0023 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0006 | 0.0051 | 0.0002 | 0.0021 | 0.0011 | 0.0016 | 0.001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 |
Endosome | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0.0006 | 0.031 | 0.0045 | 0.0174 | 0.0193 | 0.0299 | 0.0059 | 0.0017 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0072 | 0.0028 | 0.0072 | 0.0081 | 0.0159 | 0.0012 | 0.0007 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0 | 0 | 0 | 0 | 0 | 0 | 0.017 | 0.0003 | 0.0028 | 0.0089 | 0.0303 | 0.0026 | 0.0012 | 0 | 0.0001 | 0 | 0 | 0 |
Mitochondria | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0013 | 0.0116 | 0.0011 | 0.0049 | 0.0161 | 0.0165 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
None | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0074 | 0.0001 | 0.0011 | 0.0003 | 0.0007 | 0.0001 | 0.0027 | 0.0053 | 0.001 | 0.0002 | 0.0007 | 0 |
Nuclear Periphery | 0.0053 | 0.0019 | 0.0032 | 0.0043 | 0.0094 | 0.0061 | 0.0267 | 0.002 | 0.0043 | 0.0108 | 0.0064 | 0.0102 | 0.002 | 0.0035 | 0.0037 | 0.0018 | 0.0027 | 0.0008 |
Nucleolus | 0.0031 | 0.0042 | 0.0039 | 0.0013 | 0.0024 | 0.0053 | 0.0036 | 0.0032 | 0.0039 | 0.0026 | 0.0083 | 0.004 | 0.0022 | 0.0025 | 0.0031 | 0.0014 | 0.0056 | 0.0043 |
Nucleus | 0.9887 | 0.9668 | 0.9868 | 0.9863 | 0.9757 | 0.9724 | 0.7888 | 0.9652 | 0.88 | 0.8949 | 0.8188 | 0.9596 | 0.9513 | 0.9638 | 0.9825 | 0.9929 | 0.9611 | 0.9929 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0133 | 0.0004 | 0.0048 | 0.0025 | 0.0142 | 0.0008 | 0.0017 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0005 | 0.0005 | 0.0028 | 0.0004 | 0.0014 | 0.0014 | 0.0152 | 0.0011 | 0.0021 | 0.0016 | 0.008 | 0.0063 | 0.0035 | 0.001 | 0.0024 | 0.0013 | 0.0247 | 0.0005 |
Vacuole | 0.0002 | 0.0016 | 0.0003 | 0.0002 | 0.0007 | 0.0015 | 0.0022 | 0.0003 | 0.0021 | 0.0025 | 0.0024 | 0.0008 | 0.0001 | 0.0001 | 0.0011 | 0 | 0.0002 | 0.0001 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0015 | 0.0001 | 0.0006 | 0.0011 | 0.0016 | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.998 | 15.7191 | 19.6349 | 21.9458 | 21.8487 | 15.5241 | 18.3439 | 18.1467 | 22.0246 | 24.4778 |
Translational Efficiency | 0.8918 | 0.9111 | 0.7259 | 0.689 | 0.607 | 0.863 | 0.7194 | 0.5687 | 0.5483 | 0.5374 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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