Standard name
Human Ortholog
Description Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.13 0.15 0.12 0.18 0.23 0.15 0.3 0.21 0.14 0.2 0.08 0.27 – – – 0.11 0.17 0.16 0.2 0.15 0.17 0.24 0.21 0.11
Bud 0 0 0 0.06 0.05 0 0.08 0.09 0.05 0.05 0 0.06 – – – 0 0.05 0 0 0 0 0 0.12 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.05 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0.06 0.2 0.08 0.09 0.09 0.12 0.05 0 0.09 0 0.09 – – – 0.17 0.18 0.1 0 0 0 0 0 0
Cytoplasm 0.74 0.7 0.68 0.58 0.48 0.38 0.35 0.47 0.47 0.39 0.5 0.4 – – – 0.57 0.56 0.52 0.53 0.56 0.36 0.26 0.07 0.26
Endoplasmic Reticulum 0.13 0.22 0.2 0.25 0.15 0.28 0.35 0.16 0.25 0.15 0.13 0.15 – – – 0 0 0 0.07 0.07 0.15 0.06 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.17 0 0.13
Golgi 0.06 0 0 0 0.09 0 0.1 0.07 0 0.06 0 0 – – – 0.17 0.15 0.24 0 0 0 0 0.05 0.05
Mitochondria 0 0 0 0 0 0.18 0.07 0.15 0.2 0.29 0.56 0.32 – – – 0.06 0 0 0 0 0.11 0.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0.05 0.06
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0.12 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0 0 0 0 0 0 0 – – – 0 0.1 0.1 0 0 0.05 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 32 51 3 21 21 20 57 44 24 32 9 45 – – – 6 14 9 53 60 13 2 1 2
Bud 9 14 1 7 5 4 15 18 8 8 4 10 – – – 0 4 0 5 17 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 12 20 2 0 0 0
Bud Site 0 0 0 0 0 0 1 1 1 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 10 22 5 10 8 12 22 11 7 15 2 15 – – – 9 15 6 5 3 2 0 0 0
Cytoplasm 176 239 17 69 45 52 67 99 78 64 59 66 – – – 31 47 30 139 224 29 2 0 5
Endoplasmic Reticulum 30 74 5 30 14 38 66 33 41 25 15 25 – – – 2 3 1 19 27 12 0 0 1
Endosome 0 0 0 0 1 2 1 8 4 3 0 4 – – – 1 0 0 10 10 1 1 0 2
Golgi 14 4 0 2 8 3 19 15 4 9 2 4 – – – 9 13 14 4 7 1 0 0 1
Mitochondria 9 0 0 1 2 24 14 31 34 47 67 53 – – – 3 2 0 8 7 9 1 0 0
Nucleus 0 0 0 1 0 4 0 1 2 1 0 4 – – – 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 2 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 1 – – – 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 2 8 2 4 8 3 7 2 0 – – – 1 8 6 1 14 4 0 0 1
Unique Cell Count 238 343 25 119 93 136 189 210 167 163 119 166 54 84 58 265 398 81 11 7 21
Labelled Cell Count 280 404 31 143 113 161 266 271 206 211 161 227 62 106 67 265 398 81 11 7 21


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.8 12.0 11.9 11.6 11.7 9.8 10.1 9.7 8.5 8.6 7.6 8.1 – – – 18.4 17.8 16.8 – – –
Std Deviation (1e-4) 1.7 2.0 1.1 1.9 1.7 2.1 1.7 1.6 1.5 1.8 1.6 1.8 – – – 3.6 2.8 3.9 – – –
Intensity Change (Log2) – – – -0.03 -0.02 -0.27 -0.24 -0.3 -0.49 -0.46 -0.65 -0.55 – – – 0.63 0.58 0.5 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 1.2 0 1.9 1.1 0 0 0 1.6 – – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm -0.9 -1.7 -2.8 -3.1 -2.0 -2.0 -2.7 -1.7 -2.7 – – – -0.9 -1.1 -1.4
Endoplasmic Reticulum 0.6 0 0.8 1.5 0 0.5 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 0 0 3.1 5.1 3.3 – – – 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.6562 8.4521 8.0671 7.2526 7.557 7.7195 5.0456 7.4136 6.9136 5.7715 6.9444 6.8375 6.6855 8.3832 8.4613 8.1611 7.1006 8.2116
Actin 0.2114 0.123 0.227 0.1924 0.2997 0.1479 0.1359 0.1043 0.2188 0.1272 0.3711 0.1427 0.2412 0.1184 0.2247 0.1775 0.2406 0.1694
Bud 0.0253 0.0211 0.1085 0.0456 0.0571 0.0102 0.0178 0.0132 0.0678 0.0535 0.0217 0.0071 0.0297 0.036 0.136 0.0586 0.0297 0.0089
Bud Neck 0.0055 0.0031 0.0039 0.0036 0.0035 0.1072 0.0096 0.0041 0.0079 0.0026 0.0061 0.125 0.0065 0.0051 0.0063 0.0025 0.0019 0.1184
Bud Periphery 0.043 0.0103 0.1391 0.0661 0.0799 0.0129 0.023 0.0063 0.084 0.0823 0.0296 0.0168 0.0394 0.0173 0.1271 0.1169 0.0229 0.0098
Bud Site 0.0343 0.1318 0.0617 0.0055 0.0153 0.0096 0.0846 0.2275 0.0282 0.0082 0.0041 0.0035 0.0393 0.1403 0.0434 0.0038 0.0053 0.0039
Cell Periphery 0.0048 0.0032 0.0028 0.0022 0.0021 0.0052 0.0042 0.0037 0.0017 0.0021 0.001 0.0018 0.0023 0.0024 0.0018 0.0017 0.0006 0.0013
Cytoplasm 0.3305 0.5314 0.3137 0.4601 0.3071 0.3608 0.3363 0.5141 0.3467 0.4141 0.3283 0.4181 0.3637 0.5819 0.3634 0.5074 0.351 0.4081
Cytoplasmic Foci 0.0583 0.0576 0.0264 0.0306 0.0391 0.0623 0.0888 0.0406 0.0305 0.0549 0.0555 0.073 0.0536 0.0275 0.02 0.0197 0.0476 0.0714
Eisosomes 0.0012 0.0002 0.0003 0.0001 0.0002 0.0006 0.0005 0.0001 0.0003 0.0003 0.0002 0.0002 0.0005 0.0001 0.0001 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.0203 0.0038 0.0049 0.0066 0.0023 0.0114 0.0156 0.0053 0.0063 0.0054 0.0045 0.0097 0.0239 0.0032 0.0041 0.0096 0.0098 0.0105
Endosome 0.0966 0.0576 0.0326 0.0904 0.082 0.0875 0.1144 0.0324 0.0462 0.0933 0.0621 0.0695 0.0723 0.0274 0.024 0.0483 0.129 0.0701
Golgi 0.0899 0.0414 0.036 0.0388 0.0851 0.132 0.0978 0.0311 0.0405 0.0935 0.098 0.0913 0.0759 0.0276 0.0227 0.0196 0.0724 0.1022
Lipid Particles 0.009 0.0004 0.0033 0.0024 0.001 0.0034 0.0156 0.0007 0.0013 0.005 0.0002 0.0019 0.0083 0.0011 0.0042 0.0014 0.0176 0.0006
Mitochondria 0.0396 0.0031 0.024 0.0364 0.012 0.0333 0.0157 0.0024 0.0154 0.0184 0.0104 0.0131 0.0135 0.0015 0.005 0.0037 0.033 0.0164
None 0.0035 0.0014 0.003 0.0015 0.0015 0.0015 0.007 0.0011 0.0052 0.0014 0.0008 0.0024 0.0038 0.0014 0.0034 0.0011 0.0026 0.0006
Nuclear Periphery 0.0051 0.0004 0.0008 0.0012 0.0006 0.001 0.0025 0.0006 0.0067 0.0008 0.0003 0.0007 0.01 0.0005 0.0006 0.0025 0.0138 0.0003
Nucleolus 0.0008 0.0001 0.0007 0.0001 0.0001 0.0002 0.0005 0.0001 0.002 0.0001 0 0.0001 0.0002 0.0001 0.0004 0.0001 0.0003 0.0001
Nucleus 0.0025 0.001 0.0009 0.0009 0.0007 0.0009 0.0016 0.001 0.0737 0.0006 0.0003 0.014 0.0014 0.0018 0.0014 0.0011 0.0037 0.0005
Peroxisomes 0.0066 0.0032 0.0034 0.002 0.0019 0.0035 0.0188 0.0054 0.0033 0.0233 0.0031 0.0052 0.0059 0.0017 0.0023 0.0024 0.0046 0.0027
Punctate Nuclear 0.0015 0.0003 0.0008 0.0004 0.0004 0.0004 0.0027 0.0005 0.0063 0.0003 0.0001 0.0005 0.0015 0.0004 0.0005 0.0004 0.0013 0.0003
Vacuole 0.0079 0.0049 0.0046 0.0093 0.0054 0.0063 0.0056 0.0048 0.0054 0.0079 0.0016 0.0023 0.0044 0.0036 0.0064 0.0151 0.0065 0.0025
Vacuole Periphery 0.0024 0.0008 0.0015 0.0038 0.0027 0.0018 0.0015 0.0007 0.0018 0.0048 0.001 0.0011 0.0026 0.0006 0.0023 0.0063 0.0058 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.7306 16.4396 13.0122 18.7798 21.0618 16.5419 21.3317 19.4459 17.8611 27.0825
Translational Efficiency 0.6362 0.8229 0.7527 0.4411 0.6899 1.2464 0.6249 0.5947 0.6118 0.6361

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Ecm25

Ecm25


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm25-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available