Standard name
Human Ortholog
Description Major mitochondrial nuclease; has RNAse and DNA endo- and exonucleolytic activities; roles in mitochondrial recombination, apoptosis and maintenance of polyploidy; involved in fragmentation of genomic DNA during PND (programmed nuclear destruction); encodes ortholog of mammalian endoG
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.07 0.08 0 0 0 0 0 0.05
Bud 0 0 – 0 0 0 0 0.06 0.06 0 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.08 0 0 0 0 0 0
Cytoplasm 0.47 0.44 – 0.36 0.62 0.63 0.46 0.48 0.41 0.4 0.28 0.34 0.57 0.81 0.86 0.6 0.55 0.58 0.13 0.07 0.11 0 0.1 0.12
Endoplasmic Reticulum 0 0.15 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 – 0.08 0.09 0.05 0 0 0 0 0 0 0.14 0.08 0.07 0.12 0.17 0.15 0.09 0.11 0.06 0.09 0.08 0.08
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.54 0.31 – 0.59 0.2 0.35 0.52 0.5 0.62 0.56 0.78 0.67 0.11 0 0 0.05 0.08 0.06 0.58 0.58 0.66 0.66 0.57 0.51
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0.06 0.08 0.08 0.13 0.06 0.11 0 0.06 0 0 0 0.07 0.06 0 0 0 0 0 0.05 0.06
Vac/Vac Membrane 0.07 0.1 – 0.05 0.08 0 0 0 0 0 0 0 0.13 0.06 0.06 0.14 0.15 0.12 0.05 0.1 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 7 – 0 0 1 0 0 0 1 0 0 22 12 1 10 7 19 4 3 0 14 40 53
Bud 2 1 – 2 5 8 9 20 18 12 21 12 9 9 4 4 2 8 1 4 0 4 19 16
Bud Neck 0 0 – 0 2 3 4 5 3 7 1 4 0 1 1 1 0 0 4 3 1 9 13 24
Bud Site 0 0 – 1 0 1 6 5 0 1 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 – 1 3 2 1 0 0 3 2 1 13 24 13 21 6 19 0 0 0 2 6 17
Cytoplasm 86 58 – 33 122 125 148 153 115 107 68 81 241 649 794 177 57 145 30 14 6 19 87 117
Endoplasmic Reticulum 8 19 – 3 1 0 0 0 0 2 0 1 17 15 3 10 4 17 9 4 0 7 15 8
Endosome 3 5 – 7 18 10 7 3 4 4 0 0 58 64 64 36 17 37 21 21 3 40 70 77
Golgi 0 0 – 0 1 0 0 0 0 0 0 0 2 1 1 1 1 1 1 1 0 4 11 11
Mitochondria 99 40 – 54 39 69 166 161 176 152 193 158 46 10 7 15 8 15 142 110 39 300 523 504
Nucleus 0 1 – 2 2 1 2 6 5 7 3 8 0 0 0 4 2 2 0 0 0 0 1 2
Nuclear Periphery 0 1 – 1 0 0 0 0 0 4 1 0 0 0 1 0 0 0 1 0 0 1 2 3
Nucleolus 0 0 – 0 1 0 1 0 1 0 1 0 0 1 0 2 5 3 0 0 0 1 4 4
Peroxisomes 0 0 – 0 4 2 0 2 1 0 1 1 9 9 23 2 0 1 2 0 0 9 8 5
SpindlePole 0 1 – 1 12 16 26 41 18 30 10 14 12 13 19 20 6 4 4 2 0 13 42 58
Vac/Vac Membrane 12 13 – 5 15 7 9 7 8 6 5 9 55 48 53 40 15 30 12 19 2 19 40 35
Unique Cell Count 182 131 92 197 197 319 321 283 270 246 236 423 799 921 295 103 248 244 191 60 459 916 980
Labelled Cell Count 215 148 110 225 245 379 403 349 336 308 290 484 856 984 343 130 301 244 191 60 459 916 980


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.2 – 5.2 5.6 4.3 4.0 4.4 3.7 4.0 3.4 3.8 6.3 5.7 5.4 6.7 6.9 6.9 5.4 5.2 5.3
Std Deviation (1e-4) 0.5 0.8 – 1.1 2.6 1.8 2.0 1.9 1.4 1.7 1.2 1.4 1.1 1.1 1.2 1.4 1.4 1.3 1.0 1.1 1.1
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5325 1.301 1.1812 0.7053 0.3339 1.0943 1.1613 2.08 1.9034 1.6016 1.4086 1.7495 0.5259 1.5339 1.4005 1.2323 0.7797 0.8841
Actin 0.0088 0.0136 0.0085 0.0062 0.0081 0.0079 0.0312 0.0199 0.0244 0.0336 0.0264 0.0107 0.0322 0.0242 0.0371 0.0232 0.0026 0.02
Bud 0.0038 0.0064 0.0053 0.0056 0.021 0.0018 0.0027 0.0118 0.004 0.009 0.0064 0.0023 0.0036 0.01 0.0052 0.0075 0.0022 0.0018
Bud Neck 0.0017 0.0018 0.0033 0.0042 0.0014 0.0048 0.0147 0.0026 0.0041 0.0295 0.0019 0.0151 0.0017 0.0015 0.003 0.0013 0.0009 0.0077
Bud Periphery 0.0059 0.0084 0.0091 0.0077 0.0331 0.0032 0.0045 0.0127 0.0059 0.0308 0.0244 0.0056 0.0079 0.0079 0.0083 0.0091 0.0039 0.0092
Bud Site 0.0112 0.0309 0.0255 0.0085 0.0076 0.0017 0.0134 0.0617 0.0191 0.0072 0.0037 0.0033 0.0079 0.0406 0.0111 0.0043 0.0017 0.0013
Cell Periphery 0.0014 0.0017 0.0044 0.0007 0.0019 0.0009 0.0013 0.0014 0.0064 0.0024 0.0009 0.0015 0.0017 0.002 0.0015 0.0004 0.0005 0.0055
Cytoplasm 0.1048 0.1897 0.1496 0.1582 0.1452 0.149 0.101 0.1762 0.1238 0.1925 0.0857 0.141 0.0633 0.1399 0.1074 0.0296 0.0268 0.1008
Cytoplasmic Foci 0.1357 0.1392 0.1391 0.1402 0.113 0.1457 0.1072 0.1413 0.1247 0.162 0.1936 0.1069 0.0835 0.1242 0.0995 0.1511 0.0789 0.1126
Eisosomes 0.0005 0.0007 0.0004 0.0001 0.0004 0.0008 0.0007 0.0003 0.0009 0.0009 0.0004 0.0002 0.0008 0.0012 0.0006 0.0002 0.0001 0.0005
Endoplasmic Reticulum 0.0131 0.0121 0.0089 0.0085 0.0109 0.0096 0.0144 0.0061 0.0054 0.0054 0.0048 0.01 0.0227 0.0181 0.0259 0.0018 0.0113 0.012
Endosome 0.2821 0.2201 0.199 0.2582 0.1763 0.2208 0.2722 0.1813 0.2181 0.138 0.2593 0.2344 0.2574 0.2564 0.2029 0.2741 0.2711 0.2335
Golgi 0.0823 0.0807 0.057 0.0539 0.065 0.0697 0.085 0.1008 0.109 0.0828 0.0867 0.0935 0.1382 0.1318 0.147 0.1901 0.1604 0.1548
Lipid Particles 0.0252 0.0069 0.0114 0.0143 0.0221 0.0127 0.0286 0.0047 0.0148 0.0093 0.0351 0.0128 0.0218 0.0042 0.0112 0.0018 0.0196 0.0057
Mitochondria 0.1947 0.1564 0.2547 0.2484 0.26 0.2305 0.168 0.1887 0.2549 0.1936 0.1588 0.2644 0.2489 0.1838 0.2779 0.2808 0.3876 0.2974
None 0.0627 0.0801 0.0721 0.0248 0.0642 0.0738 0.0618 0.0527 0.0274 0.0353 0.0065 0.0467 0.0426 0.0174 0.0078 0.0011 0.0019 0.0043
Nuclear Periphery 0.0048 0.0052 0.003 0.0056 0.0017 0.0026 0.0117 0.0011 0.0012 0.003 0.0051 0.0022 0.0086 0.002 0.0046 0.0004 0.0089 0.0035
Nucleolus 0.0047 0.0008 0.002 0.0008 0.0025 0.0019 0.0018 0.0006 0.0008 0.001 0.0006 0.0008 0.001 0.0004 0.0003 0.0001 0.0004 0.0003
Nucleus 0.0046 0.0042 0.0035 0.0039 0.0024 0.006 0.0079 0.0016 0.0014 0.0031 0.0015 0.0043 0.0024 0.0017 0.0013 0.0005 0.001 0.0009
Peroxisomes 0.0213 0.0144 0.0159 0.02 0.0399 0.0342 0.0335 0.0177 0.0342 0.0359 0.0816 0.0212 0.0132 0.0067 0.0277 0.0113 0.0048 0.0087
Punctate Nuclear 0.0049 0.0015 0.0038 0.004 0.0051 0.0042 0.005 0.0008 0.0014 0.0074 0.0048 0.0011 0.0067 0.0006 0.0012 0.0003 0.0018 0.0022
Vacuole 0.0186 0.0193 0.0182 0.0163 0.0121 0.012 0.0241 0.0115 0.0125 0.0129 0.0083 0.0147 0.0249 0.0186 0.0128 0.0058 0.0065 0.0101
Vacuole Periphery 0.007 0.006 0.0053 0.0098 0.0059 0.006 0.0093 0.0042 0.0054 0.0044 0.0036 0.0076 0.009 0.0069 0.0059 0.0052 0.007 0.0073

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.452 33.8755 18.4832 25.9844 41.9068 40.1016 20.3998 20.3978 15.9712 35.4938
Translational Efficiency 1.1565 0.7628 1.3882 0.9012 0.7184 1.0237 1.5194 1.1335 1.4131 1.1244

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2027 364 2672 111 1695 1730 148 1329 3722 2094 2820 1440

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 742.67 832.09 944.26 1019.26 693.73 758.60 1024.94 964.40 720.38 771.37 948.49 968.63
Standard Deviation 92.27 110.83 121.37 122.97 92.85 112.90 109.55 143.89 95.69 115.94 122.11 143.14
Intensity Change Log 2 — 0.164018 0.346463 0.456729 — 0.128965 0.563093 0.475257 — 0.147195 0.455152 0.465707

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000670 0.004660 0.008034 0.024468 0.001225 0.001729 0.002183 0.031099 0.000923 0.002238 0.007727 0.030588
Bud Neck 0.032575 0.039601 0.032009 0.012050 0.035872 0.036571 0.011032 0.012175 0.034077 0.037097 0.030908 0.012166
Bud Site 0.027665 0.107588* 0.167738* 0.193415* 0.028697 0.055431 0.026643 0.227799* 0.028135 0.064497 0.160333* 0.225148*
Cell Periphery 0.000225 0.001477 0.000213 0.000442 0.000377 0.000372 0.000357 0.000454 0.000295 0.000564 0.000220 0.000453
Cytoplasm 0.115192* 0.063651 0.207544* 0.088573 0.156825* 0.075537 0.306693* 0.115438* 0.134151* 0.073471 0.212748* 0.113367*
Cytoplasmic Foci 0.293460* 0.228951* 0.139156 0.229476* 0.355180* 0.234987* 0.101879 0.168122 0.321567* 0.233938* 0.137200 0.172852
Eisosomes 0.000227 0.000204 0.000096 0.000123 0.000292 0.000208 0.000170 0.000147 0.000257 0.000208 0.000100 0.000145
Endoplasmic Reticulum 0.005841 0.001807 0.004238 0.003904 0.005426 0.001443 0.009561 0.001857 0.005652 0.001506 0.004518 0.002015
Endosome 0.098885 0.127591 0.054011 0.080918 0.078286 0.113877 0.037712 0.049604 0.089505 0.116261 0.053155 0.052018
Golgi 0.062623 0.087176 0.036525 0.037488 0.054797 0.100669 0.000698 0.072513 0.059059 0.098324 0.034645 0.069813
Lipid Particles 0.023989 0.005355 0.002353 0.004165 0.024265 0.008540 0.010579 0.003526 0.024115 0.007987 0.002785 0.003575
Mitochondria 0.176355* 0.143734* 0.075432 0.002957 0.118877* 0.230229* 0.000402 0.045083 0.150180* 0.215193* 0.071494 0.041836
Mitotic Spindle 0.006058 0.026078 0.045227 0.122481* 0.005536 0.015558 0.012805 0.138496* 0.005821 0.017386 0.043526 0.137262*
None 0.001674 0.000346 0.000327 0.000085 0.001257 0.000819 0.003270 0.000217 0.001484 0.000737 0.000481 0.000207
Nuclear Periphery 0.001106 0.000151 0.000717 0.000268 0.000494 0.000170 0.002183 0.000106 0.000828 0.000167 0.000794 0.000118
Nuclear Periphery Foci 0.001286 0.000565 0.001024 0.003385 0.000744 0.000446 0.009517 0.001205 0.001039 0.000466 0.001470 0.001373
Nucleolus 0.002126 0.001714 0.000582 0.000232 0.002468 0.001490 0.004067 0.000206 0.002282 0.001529 0.000765 0.000208
Nucleus 0.020625 0.005992 0.013600 0.005372 0.027546 0.006093 0.130066* 0.004084 0.023777 0.006075 0.019713 0.004183
Peroxisomes 0.018835 0.067407 0.026189 0.067450 0.025985 0.041599 0.007169 0.065837 0.022091 0.046085 0.025190 0.065961
Vacuole 0.086495 0.066257 0.169491 0.120540 0.062212 0.056896 0.320894* 0.054089 0.075437 0.058523 0.177437 0.059212
Vacuole Periphery 0.024087 0.019695 0.015492 0.002208 0.013636 0.017338 0.002122 0.007942 0.019328 0.017748 0.014791 0.007500

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.55 -10.62 -3.63 -2.83 -2.30 -1.15 -1.53 -12.89 -12.76 -12.36 -3.95 -10.06 -13.62 -12.89 -9.95
Bud Neck -1.47 0.22 8.25 5.79 9.44 -0.30 7.97 11.20 10.88 -0.80 -1.37 1.73 14.16 12.22 12.68
Bud Site -9.86 -32.15* -6.76 -2.66 -0.22 -9.01 0.68 -27.06* -22.36 -23.21* -13.28 -32.43 -28.30 -21.40 -7.78
Cell Periphery -1.38 1.02 -2.06 1.18 -2.26 0.04 0.36 -0.48 -0.47 -1.28 -1.49 2.00 -2.27 0.78 -4.78
Cytoplasm 7.05 -16.82 1.01 -2.06 7.00 13.54 -5.55 4.63 -7.99 7.02 14.14 -15.46 2.10 -8.57 14.55
Cytoplasmic Foci 6.03 29.50 5.13 1.40 -3.66 16.81 27.89 27.12 10.75 -8.16 15.86 42.24 27.23 10.86 -6.49
Eisosomes 0.92 13.51 5.77 3.18 -1.41 3.92 5.17 8.04 4.85 1.59 3.41 15.74 10.45 5.52 -6.30
Endoplasmic Reticulum 6.82 2.10 1.28 -1.98 0.24 5.55 -3.30 4.91 -2.07 6.70 9.00 1.61 7.61 -2.62 6.99
Endosome -3.41 14.50 3.12 4.87 -2.21 -7.71 8.65 8.98 16.21 -2.83 -6.92 15.25 14.26 17.60 1.03
Golgi -3.38 10.43 5.20 6.37 0.74 -11.02 22.81 -2.43 7.46 -19.00* -11.25 12.20 -0.84 8.23 -8.58
Lipid Particles 9.84 15.92 11.19 -0.02 -2.93 9.59 8.56 13.44 4.87 5.16 13.36 21.43 18.53 4.66 -2.50
Mitochondria 2.54 17.86 32.87* 11.78 23.16* -13.71 25.46 14.07 25.85 -12.99 -9.41 18.65 24.66 27.00 7.56
Mitotic Spindle -4.92 -15.15 -5.81 -4.85 -4.08 -6.20 -2.04 -19.17 -17.58 -11.28 -8.08 -15.47 -20.06 -18.07 -13.65
None 4.98 5.35 6.00 2.17 4.13 2.34 -1.46 6.58 3.43 2.59 4.01 6.63 7.51 3.50 1.97
Nuclear Periphery 5.08 1.45 3.03 -2.87 1.75 6.04 -8.18 5.84 -0.56 9.78 6.47 -0.20 6.08 -1.47 4.98
Nuclear Periphery Foci 1.66 0.48 -2.59 -3.13 -2.80 2.14 -5.00 -2.52 -3.25 4.45 2.42 -1.94 -2.24 -4.03 -0.92
Nucleolus 0.45 6.57 6.19 1.30 0.97 1.59 -0.24 4.96 2.56 2.44 1.68 6.40 8.32 2.90 3.89
Nucleus 8.04 2.98 2.09 -1.16 1.07 12.03 -7.79 11.63 -0.53 9.46 14.58 1.21 12.99 -0.89 10.49
Peroxisomes -7.49 -4.03 -4.56 0.92 -3.83 -6.53 11.20 -10.86 -5.54 -16.69 -10.94 -1.43 -13.06 -4.44 -12.10
Vacuole 2.63 -19.35 -3.61 -4.27 0.57 1.44 -13.70 -2.76 -3.85 12.94 5.35 -25.66 -1.62 -4.94 18.57
Vacuole Periphery 1.66 7.39 19.70 7.20 15.58 -3.21 14.26 6.00 8.46 -6.16 1.43 5.66 12.56 9.71 7.04
Standard name
Human Ortholog
Description Major mitochondrial nuclease; has RNAse and DNA endo- and exonucleolytic activities; roles in mitochondrial recombination, apoptosis and maintenance of polyploidy; involved in fragmentation of genomic DNA during PND (programmed nuclear destruction); encodes ortholog of mammalian endoG
Localization
Cell Percentages mitochondrion (56%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Nuc1

Nuc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nuc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available