Standard name | |
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Human Ortholog | |
Description | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides are active against a wide variety of wine-related yeasts and bateria; TDH2 has a paralog, TDH3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 4 | 4 | 2 | 2 | 4 | 0 | 4 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 3 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 2 | 5 | 2 | 0 | 4 | 4 | 8 | 6 | 1 | 7 | 141 | 251 | 296 | 4 | 2 | 6 | 0 | 0 | 0 | 10 | 52 | 54 |
Cytoplasm | 195 | 88 | 128 | 188 | 314 | 309 | 357 | 321 | 293 | 275 | 255 | 237 | 222 | 267 | 198 | 326 | 87 | 271 | 155 | 96 | 122 | 442 | 586 | 593 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 2 | 17 | 25 | 10 | 19 | 9 | 10 | 0 | 0 | 0 | 16 | 6 | 13 | 3 | 3 | 0 | 13 | 12 | 15 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 1 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 2 | 4 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 2 | 0 | 1 | 0 | 2 | 1 | 1 |
Unique Cell Count | 195 | 90 | 129 | 191 | 319 | 311 | 378 | 360 | 313 | 304 | 268 | 261 | 320 | 456 | 436 | 332 | 90 | 280 | 166 | 113 | 131 | 490 | 674 | 681 |
Labelled Cell Count | 195 | 91 | 130 | 194 | 322 | 312 | 380 | 362 | 318 | 307 | 269 | 264 | 363 | 522 | 497 | 349 | 97 | 297 | 166 | 113 | 131 | 490 | 674 | 681 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 402.6 | 368.1 | 369.9 | 376.3 | 336.6 | 273.0 | 240.2 | 220.6 | 214.5 | 205.9 | 192.4 | 192.5 | 595.0 | 641.9 | 646.8 | 290.8 | 333.4 | 322.3 | 523.2 | 577.4 | 584.9 |
Std Deviation (1e-4) | 76.0 | 114.7 | 93.3 | 82.0 | 76.8 | 66.1 | 59.1 | 54.5 | 56.4 | 51.8 | 50.6 | 49.7 | 116.1 | 123.8 | 130.6 | 103.8 | 119.1 | 114.3 | 115.3 | 118.1 | 130.1 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.14 | -0.44 | -0.62 | -0.75 | -0.79 | -0.85 | -0.94 | -0.94 | 0.69 | 0.8 | 0.81 | -0.35 | -0.15 | -0.2 | 0.5 | 0.64 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.7 | 10.8 | 13.3 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -0.7 | 0.2 | -2.3 | -3.6 | -2.5 | -3.3 | -2.1 | -3.2 | -6.9 | -8.7 | -10.9 | -0.8 | -1.4 | -1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 3.1 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 295.0947 | 361.1866 | 311.6672 | 313.0977 | 255.0932 | 336.1491 | 294.1918 | 393.9647 | 372.127 | 368.2975 | 311.0985 | 375.154 | 289.7679 | 342.9718 | 305.4889 | 299.6234 | 263.6177 | 390.9119 |
Actin | 0.0038 | 0.0002 | 0.0008 | 0.0025 | 0.0161 | 0.0002 | 0.0028 | 0.0002 | 0.0032 | 0.0173 | 0.0069 | 0.0006 | 0.0074 | 0.0003 | 0.0002 | 0.0006 | 0.0008 | 0.0857 |
Bud | 0.0011 | 0.0005 | 0.0055 | 0.0002 | 0.0002 | 0.0002 | 0.0035 | 0.0002 | 0.0014 | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0005 | 0.0001 | 0.0008 | 0.0008 | 0.0007 |
Bud Neck | 0.0004 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0035 | 0.0003 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0009 |
Bud Periphery | 0.0006 | 0.0001 | 0.0019 | 0.0001 | 0.0001 | 0.0001 | 0.0015 | 0 | 0.0008 | 0.0009 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0004 |
Bud Site | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0.0021 | 0.0001 | 0.0011 | 0.001 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0007 | 0.0002 |
Cell Periphery | 0.0006 | 0.0008 | 0.0011 | 0.0001 | 0.0009 | 0.0001 | 0.0014 | 0.0001 | 0.0006 | 0.0034 | 0.0004 | 0 | 0.0004 | 0.0009 | 0.0001 | 0.0012 | 0.0007 | 0.0001 |
Cytoplasm | 0.4565 | 0.7702 | 0.6105 | 0.5991 | 0.5692 | 0.6232 | 0.4417 | 0.7678 | 0.6401 | 0.5042 | 0.6625 | 0.4318 | 0.4037 | 0.7854 | 0.6077 | 0.5358 | 0.463 | 0.5157 |
Cytoplasmic Foci | 0.0061 | 0.0011 | 0.0035 | 0.0014 | 0.0383 | 0.0106 | 0.0115 | 0.0031 | 0.0112 | 0.0003 | 0.0109 | 0.006 | 0.0083 | 0.0002 | 0.0059 | 0.0003 | 0.0533 | 0.002 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0014 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0037 | 0.0005 |
Endoplasmic Reticulum | 0.002 | 0.0001 | 0.0014 | 0.0153 | 0.003 | 0.0009 | 0.0015 | 0.0001 | 0.0002 | 0.0007 | 0.0011 | 0.0001 | 0.0023 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0014 |
Endosome | 0.0017 | 0 | 0.0024 | 0.0098 | 0.0078 | 0.0089 | 0.0075 | 0 | 0.0013 | 0.0002 | 0.0488 | 0.0023 | 0.0045 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0074 |
Golgi | 0.0002 | 0 | 0.0001 | 0.0007 | 0.0078 | 0.0002 | 0.0016 | 0 | 0.0015 | 0.0004 | 0.0077 | 0.0014 | 0.0008 | 0 | 0.0001 | 0 | 0.0005 | 0.0063 |
Lipid Particles | 0.0006 | 0.0001 | 0.0007 | 0.0002 | 0.0148 | 0.0007 | 0.0046 | 0.0001 | 0.0027 | 0.0001 | 0.0214 | 0.0181 | 0.0015 | 0 | 0.0014 | 0.0001 | 0.0168 | 0.0058 |
Mitochondria | 0.0057 | 0.0025 | 0.0031 | 0.0008 | 0.0031 | 0.0038 | 0.0155 | 0.0015 | 0.0127 | 0.0007 | 0.0093 | 0.0009 | 0.0052 | 0.009 | 0.0046 | 0.0078 | 0.0183 | 0.0023 |
None | 0.0048 | 0.0018 | 0.0113 | 0.0006 | 0.0629 | 0.0186 | 0.013 | 0.0013 | 0.0111 | 0.0012 | 0.0148 | 0.0004 | 0.0035 | 0.001 | 0.0022 | 0.002 | 0.0132 | 0.0026 |
Nuclear Periphery | 0.0353 | 0.0125 | 0.0374 | 0.0275 | 0.0151 | 0.0245 | 0.02 | 0.0109 | 0.0125 | 0.0257 | 0.0138 | 0.0198 | 0.0279 | 0.0119 | 0.015 | 0.0235 | 0.0382 | 0.0172 |
Nucleolus | 0.0162 | 0.0059 | 0.0063 | 0.0009 | 0.0527 | 0.0025 | 0.0365 | 0.0044 | 0.0233 | 0.003 | 0.0049 | 0.0011 | 0.004 | 0.0029 | 0.0109 | 0.0237 | 0.0636 | 0.0003 |
Nucleus | 0.4583 | 0.2014 | 0.2953 | 0.3371 | 0.1923 | 0.2934 | 0.4203 | 0.2088 | 0.266 | 0.4348 | 0.1454 | 0.4953 | 0.5231 | 0.1853 | 0.3462 | 0.3986 | 0.3103 | 0.3432 |
Peroxisomes | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0068 | 0.0003 | 0.0033 | 0.0001 | 0.0049 | 0 | 0.0418 | 0.0105 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0031 | 0.0033 |
Punctate Nuclear | 0.0016 | 0.0004 | 0.0108 | 0.0011 | 0.0041 | 0.0013 | 0.0033 | 0.0005 | 0.0015 | 0.0006 | 0.0006 | 0.0098 | 0.0012 | 0.0004 | 0.0034 | 0.0013 | 0.0077 | 0.0028 |
Vacuole | 0.0022 | 0.0012 | 0.0046 | 0.0012 | 0.0017 | 0.0072 | 0.0043 | 0.0005 | 0.0021 | 0.0009 | 0.0044 | 0.0005 | 0.0017 | 0.001 | 0.0004 | 0.0013 | 0.0016 | 0.0005 |
Vacuole Periphery | 0.0018 | 0.0005 | 0.0021 | 0.0007 | 0.0012 | 0.0026 | 0.0027 | 0.0004 | 0.0012 | 0.0005 | 0.004 | 0.0005 | 0.0014 | 0.0006 | 0.0005 | 0.0017 | 0.0029 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10299.546 | 11083.1557 | 12563.7625 | 9055.2188 | 11109.0824 | 10906.5072 | 11313.9861 | 11253.8116 | 13554.1656 | 9818.8314 |
Translational Efficiency | 0.7189 | 0.7239 | 0.6685 | 0.8599 | 0.7131 | 0.7196 | 0.9364 | 0.931 | 0.7741 | 0.972 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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