Standard name
Human Ortholog
Description Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides are active against a wide variety of wine-related yeasts and bateria; TDH2 has a paralog, TDH3, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.44 0.55 0.68 0 0 0 0 0 0 0 0.08 0.08
Cytoplasm 1.0 0.98 0.99 0.98 0.98 0.99 0.94 0.89 0.94 0.9 0.95 0.91 0.69 0.59 0.45 0.98 0.97 0.97 0.93 0.85 0.94 0.9 0.87 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0.05 0.07 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 1 3 0 1 4 4 2 2 4 0 4 0 1 1 1 0 1 0 3 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 2 5 2 0 4 4 8 6 1 7 141 251 296 4 2 6 0 0 0 10 52 54
Cytoplasm 195 88 128 188 314 309 357 321 293 275 255 237 222 267 198 326 87 271 155 96 122 442 586 593
Endoplasmic Reticulum 0 0 0 0 1 2 17 25 10 19 9 10 0 0 0 16 6 13 3 3 0 13 12 15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 1 0 0 2 0 0 4 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 7 1 1 1 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 3 2 1
Nucleolus 0 0 0 0 0 0 0 8 2 4 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 1 1 0 1 1 0 1 0 0 1 2 1 2 0 1 0 2 1 1
Unique Cell Count 195 90 129 191 319 311 378 360 313 304 268 261 320 456 436 332 90 280 166 113 131 490 674 681
Labelled Cell Count 195 91 130 194 322 312 380 362 318 307 269 264 363 522 497 349 97 297 166 113 131 490 674 681


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 402.6 368.1 369.9 376.3 336.6 273.0 240.2 220.6 214.5 205.9 192.4 192.5 595.0 641.9 646.8 290.8 333.4 322.3 523.2 577.4 584.9
Std Deviation (1e-4) 76.0 114.7 93.3 82.0 76.8 66.1 59.1 54.5 56.4 51.8 50.6 49.7 116.1 123.8 130.6 103.8 119.1 114.3 115.3 118.1 130.1
Intensity Change (Log2) 0.02 -0.14 -0.44 -0.62 -0.75 -0.79 -0.85 -0.94 -0.94 0.69 0.8 0.81 -0.35 -0.15 -0.2 0.5 0.64 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 8.7 10.8 13.3 0 0 0
Cytoplasm -0.6 -0.7 0.2 -2.3 -3.6 -2.5 -3.3 -2.1 -3.2 -6.9 -8.7 -10.9 -0.8 -1.4 -1.5
Endoplasmic Reticulum 0 0 0 0 3.1 0 2.9 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 295.0947 361.1866 311.6672 313.0977 255.0932 336.1491 294.1918 393.9647 372.127 368.2975 311.0985 375.154 289.7679 342.9718 305.4889 299.6234 263.6177 390.9119
Actin 0.0038 0.0002 0.0008 0.0025 0.0161 0.0002 0.0028 0.0002 0.0032 0.0173 0.0069 0.0006 0.0074 0.0003 0.0002 0.0006 0.0008 0.0857
Bud 0.0011 0.0005 0.0055 0.0002 0.0002 0.0002 0.0035 0.0002 0.0014 0.0004 0.0003 0.0002 0.0005 0.0005 0.0001 0.0008 0.0008 0.0007
Bud Neck 0.0004 0.0002 0.0002 0.0005 0.0003 0.0003 0.0006 0.0001 0.0002 0.0035 0.0003 0.0004 0.0007 0.0002 0.0001 0.0002 0.0003 0.0009
Bud Periphery 0.0006 0.0001 0.0019 0.0001 0.0001 0.0001 0.0015 0 0.0008 0.0009 0.0002 0.0001 0.0002 0.0001 0 0.0004 0.0001 0.0004
Bud Site 0.0002 0.0002 0.0006 0.0001 0.0003 0.0003 0.0021 0.0001 0.0011 0.001 0.0004 0.0001 0.0003 0.0001 0.0001 0.0003 0.0007 0.0002
Cell Periphery 0.0006 0.0008 0.0011 0.0001 0.0009 0.0001 0.0014 0.0001 0.0006 0.0034 0.0004 0 0.0004 0.0009 0.0001 0.0012 0.0007 0.0001
Cytoplasm 0.4565 0.7702 0.6105 0.5991 0.5692 0.6232 0.4417 0.7678 0.6401 0.5042 0.6625 0.4318 0.4037 0.7854 0.6077 0.5358 0.463 0.5157
Cytoplasmic Foci 0.0061 0.0011 0.0035 0.0014 0.0383 0.0106 0.0115 0.0031 0.0112 0.0003 0.0109 0.006 0.0083 0.0002 0.0059 0.0003 0.0533 0.002
Eisosomes 0.0002 0.0001 0.0002 0 0.0014 0.0001 0.0005 0 0.0002 0.0001 0.0001 0 0.001 0.0001 0.0001 0.0003 0.0037 0.0005
Endoplasmic Reticulum 0.002 0.0001 0.0014 0.0153 0.003 0.0009 0.0015 0.0001 0.0002 0.0007 0.0011 0.0001 0.0023 0.0001 0.0002 0.0001 0.0002 0.0014
Endosome 0.0017 0 0.0024 0.0098 0.0078 0.0089 0.0075 0 0.0013 0.0002 0.0488 0.0023 0.0045 0 0.0004 0.0001 0.0002 0.0074
Golgi 0.0002 0 0.0001 0.0007 0.0078 0.0002 0.0016 0 0.0015 0.0004 0.0077 0.0014 0.0008 0 0.0001 0 0.0005 0.0063
Lipid Particles 0.0006 0.0001 0.0007 0.0002 0.0148 0.0007 0.0046 0.0001 0.0027 0.0001 0.0214 0.0181 0.0015 0 0.0014 0.0001 0.0168 0.0058
Mitochondria 0.0057 0.0025 0.0031 0.0008 0.0031 0.0038 0.0155 0.0015 0.0127 0.0007 0.0093 0.0009 0.0052 0.009 0.0046 0.0078 0.0183 0.0023
None 0.0048 0.0018 0.0113 0.0006 0.0629 0.0186 0.013 0.0013 0.0111 0.0012 0.0148 0.0004 0.0035 0.001 0.0022 0.002 0.0132 0.0026
Nuclear Periphery 0.0353 0.0125 0.0374 0.0275 0.0151 0.0245 0.02 0.0109 0.0125 0.0257 0.0138 0.0198 0.0279 0.0119 0.015 0.0235 0.0382 0.0172
Nucleolus 0.0162 0.0059 0.0063 0.0009 0.0527 0.0025 0.0365 0.0044 0.0233 0.003 0.0049 0.0011 0.004 0.0029 0.0109 0.0237 0.0636 0.0003
Nucleus 0.4583 0.2014 0.2953 0.3371 0.1923 0.2934 0.4203 0.2088 0.266 0.4348 0.1454 0.4953 0.5231 0.1853 0.3462 0.3986 0.3103 0.3432
Peroxisomes 0.0002 0.0001 0.0001 0.0001 0.0068 0.0003 0.0033 0.0001 0.0049 0 0.0418 0.0105 0.0003 0 0.0002 0.0002 0.0031 0.0033
Punctate Nuclear 0.0016 0.0004 0.0108 0.0011 0.0041 0.0013 0.0033 0.0005 0.0015 0.0006 0.0006 0.0098 0.0012 0.0004 0.0034 0.0013 0.0077 0.0028
Vacuole 0.0022 0.0012 0.0046 0.0012 0.0017 0.0072 0.0043 0.0005 0.0021 0.0009 0.0044 0.0005 0.0017 0.001 0.0004 0.0013 0.0016 0.0005
Vacuole Periphery 0.0018 0.0005 0.0021 0.0007 0.0012 0.0026 0.0027 0.0004 0.0012 0.0005 0.004 0.0005 0.0014 0.0006 0.0005 0.0017 0.0029 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10299.546 11083.1557 12563.7625 9055.2188 11109.0824 10906.5072 11313.9861 11253.8116 13554.1656 9818.8314
Translational Efficiency 0.7189 0.7239 0.6685 0.8599 0.7131 0.7196 0.9364 0.931 0.7741 0.972

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides are active against a wide variety of wine-related yeasts and bateria; TDH2 has a paralog, TDH3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Tdh2

Tdh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tdh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available