Standard name
Human Ortholog
Description ATP sulfurylase; catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism; human homolog PAPSS2 complements yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.99 1.0 0.94 0.92 0.87 0.8 0.75 0.82 0.59 0.7 1.0 0.99 0.99 0.98 0.97 0.96 0.93 0.84 0.9 0.87 0.89 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0.1 0.17 0.23 0.27 0.35 0.51 0.43 0.59 0.54 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.05 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 2 3
Bud 0 0 1 0 2 0 1 2 8 3 2 6 0 1 1 0 0 0 0 0 0 0 1 5
Bud Neck 0 0 0 0 0 0 0 5 1 2 1 1 0 0 1 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 0 1 0 0 1 0 0 1 2 0 1 0 1 2 4 0 1 0 0 1 1
Cytoplasm 190 177 81 73 110 168 153 132 129 163 84 111 229 317 295 366 256 190 193 168 87 109 217 213
Endoplasmic Reticulum 2 1 0 0 1 0 0 0 0 0 1 1 0 3 1 13 7 11 0 0 1 1 1 1
Endosome 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 2 0 1 1 1 0 2 1 3
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 1 0
Mitochondria 22 7 3 7 20 42 47 57 88 85 84 86 0 3 0 7 1 2 4 8 4 3 8 9
Nucleus 0 0 0 0 1 1 0 1 0 0 1 0 0 1 0 1 1 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 3 1 4 2 2 2 0 0 1 2 0 0 1 1 0 0 1 0
Vac/Vac Membrane 0 1 0 0 0 1 1 3 2 2 0 2 0 0 0 17 19 9 0 13 0 0 1 3
Unique Cell Count 202 183 82 73 117 182 176 164 171 198 142 158 229 321 298 375 265 198 208 201 97 125 244 255
Labelled Cell Count 217 187 86 80 136 213 206 202 236 257 177 212 229 327 300 409 287 219 208 201 97 125 244 255


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.1 4.1 4.1 3.8 3.2 2.8 3.0 2.7 2.8 2.4 2.5 4.4 4.6 4.5 6.9 8.2 9.3 3.7 4.2 5.1
Std Deviation (1e-4) 0.6 1.0 2.3 2.1 1.7 1.0 1.2 0.9 0.6 1.5 0.5 1.4 1.6 2.1 1.9 2.5 3.0 3.3 0.9 1.5 1.8
Intensity Change (Log2) – – – -0.02 -0.12 -0.39 -0.55 -0.47 -0.63 -0.57 -0.76 -0.71 0.08 0.15 0.12 0.75 0.99 1.18 -0.15 0.01 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0598 0.0012 0.0102 0.0413 0.0005 0.0005 0.0494 0.0008 0.0067 0.0045 0.0003 0.0044 0.4357 0.0018 0.0007 0.0023 0.0001 0.0002
Bud 0.0003 0.0015 0.0003 0.0006 0.0001 0.0003 0.0005 0.0052 0.0027 0.0036 0.0001 0.0011 0.0106 0.0001 0.0256 0.0002 0.0001 0
Bud Neck 0.0019 0.0002 0.0008 0.0029 0.0004 0.001 0.0039 0.0002 0.0009 0.0008 0.0002 0.0007 0.0139 0.0003 0.0003 0.0004 0.0001 0.0004
Bud Periphery 0.0004 0.0007 0.0002 0.0006 0.0001 0.0001 0.0005 0.0162 0.0015 0.0022 0 0.0022 0.0178 0.0001 0.0076 0.0001 0 0
Bud Site 0.0026 0.0008 0.008 0.0004 0.0002 0.0001 0.0082 0.0042 0.0026 0.0006 0.0001 0.0003 0.0809 0.0014 0.018 0.0007 0 0
Cell Periphery 0.0006 0.0002 0.0002 0 0 0.0001 0.0005 0.0002 0.0003 0.0001 0 0.0001 0.0031 0.0003 0.0002 0.0001 0 0
Cytoplasm 0.6406 0.9139 0.8674 0.8583 0.871 0.9049 0.5386 0.7185 0.6458 0.6937 0.8844 0.7607 0.0139 0.8002 0.694 0.96 0.6797 0.8211
Cytoplasmic Foci 0.0063 0.0024 0.0078 0.0074 0.0057 0.0041 0.0371 0.004 0.0135 0.0076 0.0104 0.0144 0.0456 0.0054 0.0037 0.0028 0.0027 0.0016
Eisosomes 0.0011 0 0.0002 0.0001 0 0 0.0009 0 0.0001 0 0 0 0.0064 0 0 0 0 0
Endoplasmic Reticulum 0.0059 0.0045 0.0068 0.0014 0.0009 0.0057 0.0075 0.0012 0.0028 0.003 0.0014 0.0016 0.0173 0.0069 0.0034 0.0066 0.0005 0.0059
Endosome 0.0067 0.001 0.0094 0.0027 0.0146 0.0033 0.0241 0.0021 0.0135 0.0029 0.003 0.0078 0.0607 0.0306 0.0003 0.001 0.0007 0.0005
Golgi 0.0054 0.0002 0.0004 0.0023 0.0041 0.0001 0.0121 0.0012 0.0017 0.0007 0.0001 0.0057 0.0299 0.0007 0.0001 0.0001 0 0
Lipid Particles 0.0025 0.0001 0.0003 0.0007 0.0182 0.0001 0.0046 0 0.0009 0.0005 0 0.0036 0.06 0.0031 0.0001 0 0 0
Mitochondria 0.0027 0.0002 0.0003 0.0005 0.0089 0.0002 0.0096 0.0167 0.0006 0.0434 0.0001 0.0142 0.0139 0.0004 0.001 0.0002 0.0001 0.0001
None 0.2011 0.0627 0.0671 0.0589 0.0566 0.0445 0.2661 0.2234 0.2877 0.2209 0.0956 0.1694 0.0572 0.1055 0.2193 0.0139 0.3145 0.1633
Nuclear Periphery 0.0053 0.0025 0.0063 0.0015 0.0014 0.0031 0.0098 0.0007 0.0013 0.002 0.0007 0.0009 0.0219 0.0093 0.0015 0.0036 0.0002 0.0006
Nucleolus 0.0006 0 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0079 0.0023 0.0015 0 0 0
Nucleus 0.0486 0.0066 0.0097 0.0162 0.0101 0.0292 0.007 0.0038 0.0058 0.0096 0.0021 0.0072 0.0081 0.0155 0.0113 0.0064 0.0006 0.0057
Peroxisomes 0.0024 0.0001 0.0002 0.0023 0.0041 0.0001 0.0144 0.0001 0.0081 0.0011 0.0001 0.0034 0.0407 0.0002 0.0003 0.0001 0 0
Punctate Nuclear 0.0033 0.0005 0.0012 0.001 0.0004 0.001 0.0025 0.0005 0.0009 0.0017 0.0007 0.0012 0.0447 0.0054 0.0104 0.001 0.0001 0.0003
Vacuole 0.0015 0.0008 0.0025 0.0008 0.0018 0.0014 0.0016 0.0007 0.0021 0.0009 0.0006 0.0007 0.0065 0.0093 0.0005 0.0004 0.0003 0.0002
Vacuole Periphery 0.0003 0.0002 0.0004 0.0002 0.0008 0.0002 0.0011 0.0002 0.0006 0.0004 0.0001 0.0003 0.0031 0.0011 0.0001 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.4243 14.77 18.5843 27.2675 10.711 11.4874 12.6613 13.8044 14.1828 12.5269
Translational Efficiency 2.1776 1.5761 1.6182 1.3702 1.6778 2.8542 1.0719 0.8184 1.3754 0.7136

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description ATP sulfurylase; catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism; human homolog PAPSS2 complements yeast null mutant
Localization
Cell Percentages cytoplasm (23%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Met3

Met3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available