ORF
Human Ortholog
Description Putative protein of unknown function; localizes to endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; not an essential gene; YJR015W has a paralog, SNG1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.57 0.69 0.83 0.88 0.81 0.8 0.64 0.81 0.64 0.77 0.56 0.66 0.9 0.99 0.99 0.93 0.88 0.92 0.14 0.28 0.3 0.48 0.66 0.58
Endoplasmic Reticulum 0.32 0.23 0.07 0 0.1 0 0 0 0 0 0 0 0.09 0 0 0 0.09 0.06 0.63 0.42 0.3 0.27 0.08 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.08 0.1 0.16 0.18 0.25 0.45 0.34 0.5 0.42 0.59 0.5 0 0 0 0 0 0 0.09 0.15 0.23 0.11 0.11 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 2 0 0 0 0 0 0 0 0 0 0 1 0 4 0 4 11 8 6 6 11 14
Bud 0 1 2 0 0 0 1 2 4 4 0 1 2 1 1 3 1 0 1 3 1 7 7 10
Bud Neck 0 0 0 0 0 0 0 0 0 3 1 1 0 0 0 5 5 1 2 2 1 5 5 11
Bud Site 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 2 0 0 0 1 0 1 0 2 0 2 2 6 4 1 7 14 9 2 5 2 4 13 16
Cytoplasm 106 169 96 43 110 64 107 160 87 130 51 67 312 508 594 491 422 298 32 88 42 126 254 238
Endoplasmic Reticulum 59 55 8 1 13 3 6 0 1 0 0 0 30 2 3 21 41 20 147 129 42 72 32 32
Endosome 1 2 1 1 0 0 0 1 0 0 0 0 1 0 1 4 9 3 2 3 2 1 1 4
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 2 3 0 0 3 1
Mitochondria 30 20 12 8 25 20 75 66 69 70 54 51 3 2 0 18 17 12 21 46 32 30 43 50
Nucleus 0 1 0 0 0 0 3 5 0 1 0 1 1 1 0 3 2 3 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 2 3 1 0 0 3 0 0 0 1 3 2 0 1 0 0 1 5
Vac/Vac Membrane 2 11 0 0 1 0 1 1 0 1 0 0 1 0 1 5 31 9 5 13 3 5 5 14
Unique Cell Count 185 244 116 49 136 80 166 197 137 168 91 101 346 513 598 527 477 324 233 310 141 266 389 412
Labelled Cell Count 204 262 122 53 151 87 199 239 165 209 108 126 356 520 601 564 549 362 233 310 141 266 389 412


Cytoplasm, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.9 3.9 3.8 3.6 3.4 2.6 3.0 2.9 2.8 2.9 2.8 5.1 4.3 4.1 4.9 6.1 5.8 4.5 4.2 5.0
Std Deviation (1e-4) 0.7 0.8 0.8 0.7 0.6 1.3 0.8 1.1 1.0 1.7 1.2 1.4 1.0 1.1 1.3 1.3 1.6 1.3 1.1 1.3 1.5
Intensity Change (Log2) -0.03 -0.1 -0.19 -0.58 -0.35 -0.41 -0.45 -0.39 -0.46 0.42 0.16 0.08 0.35 0.66 0.59 0.22 0.13 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 -0.4 -0.5 -3.4 -0.3 -3.4 -1.1 -4.2 -2.8 2.2 8.1 9.1 3.6 1.7 2.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.6 0 0 -1.4 0.6 -0.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -3.2 -3.0 -2.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6293 3.7588 3.7838 3.8687 3.1757 3.4461 2.112 2.5307 2.4482 2.3064 1.6582 2.1356 3.292 3.2037 3.1538 2.8488 2.1209 2.5331
Actin 0.0008 0.0004 0.0135 0.0034 0.0118 0.0006 0.0242 0.0043 0.0149 0.0042 0.0065 0.011 0.1952 0.0035 0.0926 0.021 0.0005 0.0035
Bud 0 0.0001 0.0009 0.0002 0.0005 0 0.0011 0.0001 0.001 0.0009 0.0002 0.0002 0.0009 0.0033 0.0046 0.0013 0 0
Bud Neck 0.0001 0.0002 0.0005 0.0006 0.0011 0.0028 0.002 0.0003 0.0032 0.0017 0.0004 0.0012 0.0265 0.0003 0.0102 0.0015 0.0003 0.0009
Bud Periphery 0.0001 0.0001 0.0015 0.0003 0.0018 0.0001 0.0028 0.0001 0.003 0.0018 0.0006 0.0006 0.0014 0.0148 0.0044 0.0028 0 0.0001
Bud Site 0.0003 0.0016 0.0118 0.0019 0.0015 0.0001 0.0077 0.0136 0.0123 0.004 0.0006 0.0002 0.0183 0.0077 0.0381 0.0015 0.0001 0.0001
Cell Periphery 0.0012 0.001 0.0014 0.0008 0.0013 0.0005 0.0017 0.0012 0.0028 0.0013 0.0009 0.0006 0.0016 0.0042 0.0035 0.003 0.0003 0.0011
Cytoplasm 0.2817 0.5406 0.361 0.3442 0.4701 0.5049 0.2382 0.3662 0.2993 0.3534 0.4526 0.4012 0.3817 0.4183 0.29 0.4549 0.5415 0.4661
Cytoplasmic Foci 0.0041 0.0082 0.0132 0.0171 0.0256 0.0091 0.0338 0.0092 0.025 0.012 0.0158 0.0186 0.054 0.0122 0.026 0.0094 0.0049 0.0146
Eisosomes 0.0003 0.0001 0.0004 0.0004 0.0008 0.0001 0.0009 0.0003 0.0006 0.0002 0.0003 0.0005 0.0028 0.0005 0.0006 0.0005 0.0002 0.0002
Endoplasmic Reticulum 0.0979 0.048 0.0882 0.0781 0.0544 0.0515 0.1151 0.0624 0.1267 0.1568 0.0583 0.0604 0.1106 0.0373 0.2097 0.14 0.116 0.1293
Endosome 0.0088 0.0074 0.0114 0.0324 0.0122 0.0061 0.0295 0.0048 0.02 0.0168 0.0075 0.0093 0.0439 0.0022 0.016 0.0372 0.0041 0.0061
Golgi 0.0004 0.0004 0.0033 0.0083 0.0029 0.0003 0.0092 0.0027 0.011 0.0047 0.0066 0.0025 0.0177 0.0008 0.0223 0.0204 0.0003 0.0006
Lipid Particles 0.0026 0.0012 0.0069 0.0172 0.0047 0.0015 0.0094 0.0028 0.0115 0.0091 0.0236 0.0042 0.0253 0.0042 0.0214 0.0035 0.0014 0.002
Mitochondria 0.0003 0.0002 0.0181 0.0127 0.0112 0.0003 0.0202 0.0003 0.0077 0.0052 0.0715 0.0088 0.0032 0.0008 0.0061 0.0267 0.0002 0.0002
None 0.5851 0.3798 0.4508 0.4694 0.3714 0.4071 0.4577 0.5249 0.4453 0.4041 0.3167 0.4671 0.0691 0.4823 0.1824 0.2533 0.325 0.3701
Nuclear Periphery 0.0089 0.0034 0.008 0.0031 0.0086 0.0038 0.0073 0.0017 0.0032 0.0055 0.0018 0.0025 0.0098 0.0015 0.0031 0.0085 0.0023 0.0013
Nucleolus 0.0002 0.0002 0.0003 0.0003 0.0005 0.0001 0.0007 0.0001 0.0002 0.0003 0.0002 0.0002 0.0008 0.0004 0.0005 0.0005 0 0.0001
Nucleus 0.0024 0.0023 0.0021 0.0015 0.0041 0.0079 0.0072 0.001 0.0011 0.0022 0.0008 0.0016 0.0038 0.0013 0.0014 0.0028 0.0011 0.0008
Peroxisomes 0.0001 0.0001 0.0015 0.0014 0.0007 0.0001 0.021 0.0018 0.0062 0.0084 0.0318 0.0063 0.0126 0.0003 0.0611 0.0004 0 0.0004
Punctate Nuclear 0.0007 0.001 0.0021 0.0012 0.0079 0.0009 0.0026 0.0007 0.001 0.0022 0.0011 0.001 0.0125 0.0018 0.003 0.0024 0.0004 0.0005
Vacuole 0.0035 0.0035 0.0027 0.0044 0.0054 0.0021 0.0063 0.0016 0.0034 0.0038 0.0017 0.0017 0.0075 0.0021 0.0026 0.0057 0.0014 0.0018
Vacuole Periphery 0.0004 0.0003 0.0004 0.001 0.0016 0.0002 0.0012 0.0001 0.0004 0.0012 0.0005 0.0003 0.0009 0.0002 0.0004 0.0027 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 68.378 69.4903 63.9785 71.1636 60.6587 51.0752 77.2552 75.3041 61.3115 69.7598
Translational Efficiency 1.1215 1.3575 1.2733 0.9993 1.1505 1.6393 1.1959 0.9677 1.1483 1.2312

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
ORF
Human Ortholog
Description Putative protein of unknown function; localizes to endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; not an essential gene; YJR015W has a paralog, SNG1, that arose from the whole genome duplication
Localization
Cell Percentages ER (48%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

YJR015W

YJR015W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yjr015w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available