Standard name
Human Ortholog
Description 5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.92 1.0 0.99 0.99 0.94 0.93 0.93 0.81 0.85 0.75 0.79 0.98 0.99 1.0 – 0.95 0.98 0.85 0.86 0.86 0.87 0.87 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0 0 0 0.07 0.05 0 0.27 0.2 0.32 0.29 0 0 0 – 0.06 0.06 0.05 0.06 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 1 1 0 1 3 4
Bud 0 0 0 0 0 1 3 3 10 12 8 8 0 0 0 – 0 0 0 0 0 1 5 11
Bud Neck 0 0 6 5 5 13 16 29 14 18 10 11 3 0 1 – 1 0 0 0 0 0 2 1
Bud Site 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 1 4 1 3 5 3 1 1 1 2 0 1 1 – 1 1 0 0 0 1 1 2
Cytoplasm 91 85 190 286 325 519 632 575 443 520 339 455 354 517 533 – 134 80 107 84 162 171 359 386
Endoplasmic Reticulum 1 1 0 0 0 0 0 0 0 0 0 0 3 0 0 – 6 6 1 0 0 1 2 1
Endosome 0 0 0 0 1 0 2 0 0 2 1 0 2 1 0 – 1 0 2 0 3 1 4 6
Golgi 0 0 0 2 0 0 0 0 0 0 0 0 0 1 2 – 0 0 2 0 0 1 0 1
Mitochondria 4 11 0 3 1 36 36 18 149 120 146 166 1 0 0 – 9 5 6 5 8 5 7 12
Nucleus 0 1 0 0 1 2 2 9 6 2 5 5 0 0 0 – 1 0 0 0 0 0 2 2
Nuclear Periphery 0 3 0 0 0 0 0 0 1 1 0 1 0 0 0 – 0 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 – 1 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – 0 1 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 4 15 16 6 12 25 30 0 0 0 – 0 1 0 0 2 2 9 8
Vac/Vac Membrane 1 0 0 1 1 8 8 10 5 5 2 4 2 2 0 – 2 1 1 2 0 1 2 5
Unique Cell Count 96 92 190 289 327 550 683 617 549 609 452 578 361 520 535 141 82 127 98 189 197 413 454
Labelled Cell Count 97 101 198 301 336 588 719 663 636 694 539 684 365 522 537 156 95 127 98 189 197 413 454


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.6 5.1 4.7 5.3 4.1 4.0 4.4 3.5 3.7 3.5 3.5 4.8 4.9 4.7 4.2 5.1 5.5 5.4 5.5 5.5
Std Deviation (1e-4) 0.7 0.8 1.1 1.5 1.6 1.4 1.4 1.3 1.5 1.9 1.3 0.7 0.9 0.9 1.0 1.8 1.9 1.6 1.5 1.4 1.5
Intensity Change (Log2) – – – -0.1 0.07 -0.29 -0.33 -0.21 -0.55 -0.46 -0.52 -0.53 -0.09 -0.05 -0.1 -0.28 0.01 0.13 0.09 0.12 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.3141 -0.6704 -1.0127 -0.7493 -1.3113 -0.8553 1.4617 2.2177 1.8625 1.6555 1.7106 2.1519 -0.6784 0.1944 -0.3015 -0.3029 -0.4164 -0.1665
Actin 0.0015 0.0005 0.0002 0.0002 0.0001 0.0007 0.0007 0.0001 0.0003 0.0002 0.0002 0.0011 0.0248 0.0001 0.0221 0 0.0414 0.0045
Bud 0.0001 0.0002 0.0002 0.0006 0.0281 0.0016 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0004 0.0005 0 0.0009 0.0002
Bud Neck 0.0003 0.0001 0.0012 0.0001 0.0002 0.0005 0.0001 0 0.0001 0.0001 0.0001 0.0017 0.0007 0.0001 0.0023 0 0.0003 0.0004
Bud Periphery 0.0002 0.0002 0.0002 0.0007 0.0167 0.0011 0.0001 0.0001 0.0003 0.0001 0.0003 0.0002 0.001 0.0003 0.0008 0 0.002 0.0001
Bud Site 0.0013 0.0003 0.0064 0.0001 0.0002 0.0004 0.0005 0.0003 0.0004 0.0001 0.0001 0 0.0006 0.0033 0.0095 0 0.0008 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0 0.0009 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.1742 0.22 0.2131 0.2682 0.1878 0.262 0.0762 0.0854 0.0757 0.136 0.1988 0.1008 0.1227 0.2061 0.2217 0.1788 0.0617 0.2996
Cytoplasmic Foci 0.0109 0.0059 0.0125 0.0037 0.0065 0.0167 0.0035 0.0035 0.0023 0.0027 0.0054 0.0046 0.0068 0.0016 0.0052 0.0025 0.0177 0.0105
Eisosomes 0.0002 0.0001 0.0001 0.0001 0 0 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0002 0 0.0003 0
Endoplasmic Reticulum 0.0014 0.0013 0.0013 0.0008 0.002 0.001 0.0005 0.0003 0.0015 0.0005 0.0004 0.0002 0.0031 0.0007 0.0022 0.0007 0.0012 0.0004
Endosome 0.0023 0.0126 0.0037 0.0009 0.0012 0.0056 0.0005 0.0008 0.0006 0.0005 0.0005 0.0004 0.0073 0.0001 0.0031 0.0004 0.0051 0.0007
Golgi 0.0003 0.0006 0.0002 0.0001 0 0.0003 0.0001 0 0 0 0.0001 0.0001 0.0017 0 0.0014 0 0.004 0.0007
Lipid Particles 0.0011 0.0012 0.0004 0.0002 0.0002 0.0015 0.0002 0.0003 0.0001 0 0.0002 0.0004 0.0018 0.0001 0.0011 0.0002 0.0051 0.0011
Mitochondria 0.0005 0.0006 0.0002 0.0002 0.0002 0.0053 0.0001 0.0001 0.0001 0.0003 0.0004 0.0003 0.0012 0.0001 0.0008 0.0001 0.003 0.0055
None 0.7999 0.7468 0.7542 0.7218 0.7504 0.6962 0.9155 0.9058 0.9155 0.8573 0.7916 0.8876 0.8122 0.7855 0.7241 0.8158 0.8456 0.6713
Nuclear Periphery 0.0006 0.0008 0.0008 0.0004 0.0007 0.0006 0.0002 0.0003 0.0005 0.0002 0.0002 0.0001 0.0046 0.0002 0.0009 0.0002 0.0038 0.0002
Nucleolus 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0009 0.0001 0.0005 0 0.0002 0.0001
Nucleus 0.0022 0.0011 0.0022 0.0008 0.001 0.0022 0.0004 0.0009 0.0008 0.0009 0.0005 0.0005 0.0032 0.0007 0.0007 0.0005 0.0006 0.0009
Peroxisomes 0.0005 0.0004 0.0002 0.0001 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001 0.0004 0.0003 0.001 0 0.0013 0 0.0028 0.0021
Punctate Nuclear 0.0011 0.0006 0.0018 0.0005 0.0007 0.0018 0.0004 0.0011 0.0007 0.0005 0.0003 0.0012 0.0037 0.0003 0.0008 0.0002 0.0024 0.0014
Vacuole 0.0009 0.0055 0.0008 0.0006 0.0026 0.0014 0.0002 0.0004 0.0004 0.0003 0.0002 0.0001 0.0013 0.0002 0.0005 0.0002 0.0005 0.0002
Vacuole Periphery 0.0001 0.0012 0.0001 0.0001 0.0003 0.0003 0 0.0001 0 0 0 0 0.0007 0 0.0001 0 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 67.5554 72.4685 46.8039 61.3669 57.4191 60.016 55.633 55.407 61.4294 66.0053
Translational Efficiency 0.7996 0.6963 1.0967 0.9479 0.8562 0.9603 1.0614 1.0344 0.8889 0.7615

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1475 938 1821 1391 1606 1493 1527 1226 3081 2431 3348 2617

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 570.97 687.41 761.29 787.71 665.85 735.23 853.67 770.07 620.43 716.78 803.42 779.45
Standard Deviation 62.99 97.19 93.77 118.61 75.85 96.02 106.34 156.19 84.53 99.24 109.80 137.78
Intensity Change Log 2 — 0.267756 0.415031 0.464250 — 0.142998 0.358481 0.209792 — 0.201935 0.384863 0.332856

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000189 0.000273 0.000915 0.001000 0.000092 0.000227 0.000248 0.000311 0.000138 0.000245 0.000611 0.000677
Bud Neck 0.032011 0.022493 0.005107 0.007236 0.003763 0.030118 0.003455 0.005695 0.017287 0.027176 0.004354 0.006514
Bud Site 0.007484 0.012266 0.013882 0.035318 0.001511 0.012348 0.004679 0.024273 0.004371 0.012317 0.009685 0.030144
Cell Periphery 0.000371 0.000139 0.000113 0.000251 0.000103 0.000145 0.000150 0.000320 0.000232 0.000143 0.000130 0.000283
Cytoplasm 0.381840* 0.466132* 0.503079* 0.460258* 0.607904* 0.463370* 0.397532* 0.524135* 0.499678* 0.464436* 0.454940* 0.490183*
Cytoplasmic Foci 0.316891* 0.236488* 0.013224 0.034175 0.143762 0.227342* 0.010127 0.036481 0.226646* 0.230871* 0.011811 0.035255
Eisosomes 0.000586 0.000118 0.000023 0.000059 0.000066 0.000133 0.000039 0.000062 0.000315 0.000127 0.000030 0.000060
Endoplasmic Reticulum 0.001299 0.000783 0.001717 0.002184 0.000846 0.000541 0.003021 0.001054 0.001062 0.000634 0.002312 0.001655
Endosome 0.009343 0.019788 0.001314 0.010526 0.005056 0.016891 0.001999 0.003598 0.007108 0.018009 0.001626 0.007281
Golgi 0.008012 0.004245 0.000707 0.007026 0.000686 0.004433 0.000069 0.000565 0.004193 0.004360 0.000416 0.004000
Lipid Particles 0.026904 0.007852 0.000736 0.002001 0.003852 0.006736 0.001368 0.001792 0.014888 0.007167 0.001024 0.001903
Mitochondria 0.030467 0.008658 0.000784 0.007471 0.001195 0.007130 0.000714 0.001229 0.015209 0.007719 0.000752 0.004547
Mitotic Spindle 0.000573 0.002539 0.003948 0.041429 0.000116 0.004790 0.003373 0.011850 0.000335 0.003922 0.003686 0.027572
None 0.006766 0.003636 0.002865 0.006587 0.007048 0.003876 0.007191 0.013951 0.006913 0.003783 0.004838 0.010037
Nuclear Periphery 0.000411 0.000597 0.001768 0.001813 0.000323 0.000417 0.001858 0.001006 0.000365 0.000486 0.001809 0.001435
Nuclear Periphery Foci 0.000197 0.000534 0.000495 0.002497 0.000347 0.000257 0.001418 0.000778 0.000275 0.000364 0.000916 0.001692
Nucleolus 0.002823 0.002494 0.000291 0.000751 0.000724 0.002539 0.000484 0.000990 0.001729 0.002521 0.000379 0.000863
Nucleus 0.137531* 0.131442* 0.407289* 0.245399* 0.193853* 0.144859* 0.505504* 0.289168* 0.166890* 0.139682* 0.452084* 0.265903*
Peroxisomes 0.013392 0.011294 0.000179 0.001604 0.001314 0.012591 0.000215 0.000564 0.007096 0.012091 0.000196 0.001117
Vacuole 0.020919 0.065933 0.041382 0.126053 0.026888 0.059709 0.056435 0.080212 0.024031 0.062111 0.048247 0.104577
Vacuole Periphery 0.001988 0.002294 0.000182 0.006364 0.000551 0.001547 0.000121 0.001965 0.001239 0.001836 0.000154 0.004303

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.23 -1.00 -2.80 -2.13 0.24 -2.35 -4.23 -2.64 -1.11 0.23 -3.01 -1.39 -3.73 -2.49 0.28
Bud Neck 3.34 11.74 11.19 7.48 -1.83 -12.54 -0.16 -2.34 11.24 -2.00 -5.27 10.64 9.25 13.47 -2.68
Bud Site -2.83 -2.24 -4.33 -2.30 -2.33 -8.36 -2.96 -6.89 -2.50 -5.61 -7.47 -3.59 -8.06 -3.38 -5.33
Cell Periphery 8.80 10.11 6.96 -1.18 -2.92 -2.65 -3.40 -5.09 -3.52 -3.70 5.86 7.87 1.15 -3.46 -4.71
Cytoplasm -6.99 -8.50 0.26 6.85 8.10 15.64 31.99 14.38 -0.18 -13.21 5.18 15.14 11.07 5.32 -2.85
Cytoplasmic Foci 8.69 52.90 49.63 29.51 -7.36 -12.52 33.25 25.30 33.92 -13.18 -1.04 56.67 50.24 45.37 -14.32
Eisosomes 12.64 15.30 14.16 8.91 -16.85 -12.12 3.65 -0.91 11.38 -4.53 10.13 15.18 13.51 14.76 -13.95
Endoplasmic Reticulum 2.46 -4.62 -6.30 -10.74 -2.81 0.79 -9.91 -1.97 -5.05 13.95 1.83 -10.36 -5.40 -10.64 7.23
Endosome -6.97 11.95 3.96 9.30 -5.99 -9.42 5.24 2.95 11.55 -3.82 -11.27 12.53 4.54 14.07 -6.95
Golgi 4.10 10.13 4.96 1.27 -2.29 -4.91 6.58 1.79 5.23 -3.36 -0.16 10.22 4.56 3.72 -2.58
Lipid Particles 11.05 18.19 17.38 6.13 -6.23 -4.14 7.59 6.78 7.54 -1.47 8.49 18.69 17.69 9.66 -5.93
Mitochondria 7.79 12.09 10.29 2.98 -2.62 -6.63 2.44 0.59 6.52 -0.77 5.36 11.97 9.81 5.74 -2.72
Mitotic Spindle -1.93 -3.98 -7.33 -6.31 -6.08 -3.88 -4.31 -5.46 -3.08 -3.46 -4.25 -5.96 -9.15 -6.68 -6.95
None 3.28 5.04 1.91 -1.33 -3.00 2.94 1.39 -1.89 -3.89 -2.92 4.30 4.38 -0.54 -3.97 -3.94
Nuclear Periphery -0.89 -17.24 -12.33 -8.82 -3.60 -2.15 -30.68 -18.47 -14.97 13.41 -1.43 -31.64 -16.48 -13.31 1.26
Nuclear Periphery Foci -3.99 -7.72 -4.91 -3.36 -3.55 2.05 -11.67 -4.95 -5.58 5.00 -2.87 -13.56 -6.20 -5.30 -1.15
Nucleolus 0.30 4.28 3.58 5.07 -5.06 -3.72 4.06 -1.96 3.27 -4.60 -1.97 4.76 3.18 5.21 -6.83
Nucleus 0.74 -39.00 -19.45 -18.25 15.93 8.13 -39.84 -13.69 -19.58 21.27 6.02 -54.16 -22.82 -26.67 25.91
Peroxisomes 1.26 12.70 11.84 8.96 -3.81 -10.39 4.04 2.86 11.51 -3.30 -5.31 13.05 11.85 14.45 -4.81
Vacuole -12.98 -14.37 -30.09 -21.00 -22.91 -11.99 -17.12 -19.41 -12.08 -3.76 -17.82 -21.92 -34.94 -24.22 -19.80
Vacuole Periphery -0.71 7.60 -0.69 -0.35 -3.23 -2.87 5.96 -1.53 0.29 -2.40 -2.17 8.99 -1.54 -0.33 -4.00
Standard name
Human Ortholog
Description 5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant
Localization
Cell Percentages cytoplasm (39%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mde1

Mde1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mde1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available