Standard name
Human Ortholog
Description 3-hydroxyanthranilic acid dioxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.94 0.9 0.89 0.84 0.83 – 0.71 0.7 0.71 0.66 0.99 0.99 0.98 0.94 0.96 0.99 0.93 0.89 0.94 0.94 0.88 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.06 0.11 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0 – 0.14 0.17 0.21 0.23 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0.05 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.11 0.14 0.1 0.19 – 0.2 0.18 0.17 0.14 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1
Bud 0 0 0 0 0 2 1 – 2 2 2 6 0 0 0 0 0 0 6 4 3 1 16 9
Bud Neck 0 0 0 0 0 0 0 – 1 1 0 0 0 0 0 0 0 0 0 1 1 0 0 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 4 1 2 0 4 – 1 7 3 0 0 0 1 2 2 1 0 0 0 0 2 0
Cytoplasm 250 225 210 178 203 240 403 – 175 202 190 198 420 354 378 116 107 69 238 204 204 176 368 360
Endoplasmic Reticulum 3 2 7 0 0 0 2 – 1 3 0 0 2 1 0 8 7 8 0 1 0 0 2 1
Endosome 1 0 0 0 0 0 1 – 0 0 0 1 0 0 0 2 2 0 1 1 1 0 1 2
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 0
Mitochondria 0 0 0 2 2 29 8 – 34 48 55 68 0 0 0 1 6 2 0 1 0 1 1 0
Nucleus 6 6 7 4 2 7 10 – 13 10 15 14 1 0 0 1 0 0 1 5 2 1 2 2
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 0 2 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 – 1 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 2 1 0 0 0 0 0 0 0 0 1 0 0 2 4 0
Vac/Vac Membrane 4 5 6 21 31 28 91 – 48 53 45 42 5 2 13 4 1 0 1 6 1 1 3 2
Unique Cell Count 258 231 223 198 228 286 483 246 287 268 299 424 357 385 124 111 70 258 230 219 188 416 388
Labelled Cell Count 265 239 234 206 240 306 520 278 328 310 333 428 357 392 136 125 80 258 230 219 188 416 388


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 11.3 10.7 10.5 11.0 6.7 8.5 – 7.0 7.0 6.7 6.4 13.2 12.2 15.2 6.0 5.7 5.3 11.4 11.9 11.9
Std Deviation (1e-4) 4.3 4.0 2.9 2.7 2.6 1.9 2.2 – 2.0 2.0 2.0 2.1 3.6 3.7 4.4 2.8 2.0 2.1 3.5 3.4 3.5
Intensity Change (Log2) – – – -0.03 0.04 -0.67 -0.34 – -0.61 -0.61 -0.67 -0.73 0.31 0.19 0.5 -0.84 -0.9 -1.02 0.09 0.15 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -2.0 -3.6 -3.9 – -6.5 -6.8 -6.6 -7.6 3.7 3.6 2.7 -0.2 0.9 1.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 1.5 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 0 – 5.8 6.4 7.2 7.6 0 0 0 0 0 0
Nucleus -0.7 0 -0.5 -0.9 – 1.1 0.2 1.3 0.9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 3.3 4.2 3.2 5.8 – 5.7 5.5 5.1 4.4 0 0 0.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.9712 13.3388 11.7506 10.5283 11.1959 12.2478 13.4888 16.7863 15.738 13.4615 13.4406 14.652 11.6908 17.3294 14.1081 15.3471 11.3472 14.2414
Actin 0.0026 0.0005 0.001 0.0106 0.0005 0.0036 0.004 0.0015 0.0015 0.0027 0.0005 0.0004 0.0239 0.001 0.0153 0.0313 0.0598 0.005
Bud 0.0006 0.0004 0.0014 0.0003 0.0003 0.0004 0.0003 0.002 0.0022 0.0052 0.0007 0.0003 0.0025 0.0013 0.003 0.0021 0.0007 0.0005
Bud Neck 0.0022 0.0004 0.0006 0.0012 0.0008 0.0016 0.0005 0.0009 0.0004 0.0007 0.0009 0.0012 0.0007 0.0002 0.0011 0.0005 0.0022 0.0013
Bud Periphery 0.0004 0.0001 0.0006 0.0003 0 0.0002 0.0002 0.0005 0.0022 0.0039 0.0002 0.0001 0.0012 0.0007 0.001 0.0027 0.001 0.0002
Bud Site 0.002 0.0007 0.0019 0.0005 0.0003 0.0002 0.0004 0.0034 0.0006 0.0016 0.0002 0.0001 0.0021 0.0011 0.0021 0.0007 0.0023 0.0001
Cell Periphery 0.0002 0 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0.0002 0 0 0.0002 0 0.0001 0.0002 0.0003 0
Cytoplasm 0.9035 0.9599 0.9369 0.8728 0.9349 0.8932 0.9128 0.9393 0.922 0.9277 0.9405 0.9279 0.8235 0.9696 0.8951 0.8361 0.7861 0.9265
Cytoplasmic Foci 0.007 0.0012 0.0019 0.0048 0.0057 0.0055 0.0051 0.0058 0.0013 0.0021 0.0018 0.002 0.0255 0.0065 0.0102 0.015 0.0127 0.0071
Eisosomes 0 0 0 0.0001 0 0 0.0001 0 0 0 0 0 0.0008 0 0.0001 0.0006 0.0009 0
Endoplasmic Reticulum 0.0037 0.0021 0.0016 0.0023 0.0028 0.0025 0.003 0.001 0.0018 0.0042 0.003 0.0026 0.0069 0.0005 0.0035 0.0044 0.007 0.0023
Endosome 0.0062 0.0006 0.001 0.0133 0.0029 0.0031 0.0033 0.0004 0.0007 0.0018 0.0013 0.0016 0.0164 0.0003 0.0041 0.0112 0.0234 0.0053
Golgi 0.0014 0 0 0.0075 0.0001 0.0007 0.0007 0.0001 0 0.0001 0.0001 0 0.005 0.0001 0.0026 0.0071 0.0099 0.0029
Lipid Particles 0.0012 0 0 0.0135 0.0001 0.001 0.0024 0 0 0.0001 0 0 0.006 0 0.0033 0.0035 0.017 0.0007
Mitochondria 0.0008 0.0002 0.0002 0.0038 0.0002 0.0002 0.0004 0.0002 0.0002 0.0003 0.0002 0.0002 0.0066 0.0002 0.0098 0.0165 0.0073 0.0027
None 0.015 0.0043 0.0099 0.0051 0.0021 0.0034 0.0065 0.0059 0.0017 0.0018 0.0015 0.0014 0.0174 0.002 0.0024 0.0057 0.0065 0.0027
Nuclear Periphery 0.0077 0.0026 0.0035 0.0056 0.0057 0.0073 0.0064 0.0027 0.0044 0.0056 0.0054 0.0054 0.0162 0.0011 0.0036 0.0077 0.0149 0.0035
Nucleolus 0.0004 0 0.0001 0.0003 0.0001 0.0001 0.0001 0.0004 0 0.0001 0.0001 0 0.001 0 0.0001 0.0001 0.0004 0
Nucleus 0.0391 0.0257 0.037 0.0517 0.0383 0.0694 0.0492 0.0323 0.0587 0.0373 0.041 0.0539 0.0237 0.0125 0.0262 0.0394 0.0265 0.0359
Peroxisomes 0.0004 0 0.0001 0.0023 0.0002 0.0003 0.0007 0.0001 0.0001 0.0001 0.0001 0 0.0103 0.0009 0.0125 0.0023 0.0126 0.0009
Punctate Nuclear 0.0029 0.0005 0.001 0.002 0.0033 0.0059 0.0021 0.0024 0.001 0.0014 0.0013 0.0014 0.0063 0.0015 0.0027 0.0083 0.0061 0.0015
Vacuole 0.0022 0.0006 0.001 0.0013 0.0014 0.0011 0.0014 0.001 0.0009 0.0027 0.001 0.0011 0.0022 0.0002 0.0008 0.0038 0.0015 0.0006
Vacuole Periphery 0.0004 0.0001 0.0001 0.0007 0.0002 0.0003 0.0003 0.0001 0.0001 0.0004 0.0002 0.0002 0.0018 0.0001 0.0003 0.0009 0.0009 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.9227 40.6372 31.5252 28.0315 22.7719 68.1502 47.1581 49.1609 43.7563 42.5138
Translational Efficiency 1.6144 1.4005 1.1547 1.6544 2.4382 1.8018 1.6566 1.2821 1.6957 1.4337

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1828 1573 661 101 1397 2133 323 1588 3225 3706 984 1689

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 967.98 1339.43 2079.01 1263.54 1021.91 1305.69 2117.02 1250.44 991.34 1320.01 2091.49 1251.22
Standard Deviation 204.29 332.95 346.14 256.71 236.05 300.78 337.24 289.65 220.24 315.28 343.71 287.80
Intensity Change Log 2 — 0.468570 1.102848 0.384422 — 0.353544 1.050767 0.291168 — 0.410644 1.076336 0.337284

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000171 0.000170 0.000241 0.004576 0.000188 0.000349 0.000655 0.000723 0.000178 0.000273 0.000377 0.000954
Bud Neck 0.002189 0.001075 0.001321 0.004927 0.001048 0.001685 0.002569 0.003556 0.001695 0.001426 0.001731 0.003638
Bud Site 0.005479 0.005387 0.001148 0.065781 0.004082 0.005466 0.005632 0.026015 0.004874 0.005432 0.002620 0.028393
Cell Periphery 0.000088 0.000081 0.000204 0.000252 0.000127 0.000060 0.000352 0.000103 0.000105 0.000069 0.000253 0.000112
Cytoplasm 0.797861* 0.812159* 0.406080* 0.441521* 0.809155* 0.830959* 0.475868* 0.686899* 0.802753* 0.822979* 0.428988* 0.672226*
Cytoplasmic Foci 0.055101 0.014975 0.003981 0.031564 0.041770 0.020312 0.010391 0.007470 0.049326 0.018047 0.006085 0.008911
Eisosomes 0.000016 0.000004 0.000002 0.000069 0.000015 0.000006 0.000006 0.000012 0.000016 0.000005 0.000003 0.000015
Endoplasmic Reticulum 0.001009 0.000378 0.000297 0.002010 0.001105 0.000324 0.000312 0.000644 0.001051 0.000347 0.000302 0.000725
Endosome 0.002232 0.000846 0.000144 0.042113 0.001974 0.001847 0.000354 0.002334 0.002120 0.001422 0.000213 0.004712
Golgi 0.000608 0.001035 0.000200 0.009859 0.000322 0.001891 0.000296 0.002477 0.000484 0.001527 0.000232 0.002919
Lipid Particles 0.000718 0.000372 0.000436 0.007587 0.000769 0.000423 0.001427 0.000412 0.000740 0.000401 0.000762 0.000842
Mitochondria 0.000936 0.000259 0.000436 0.001852 0.000296 0.000401 0.000286 0.001924 0.000659 0.000341 0.000387 0.001920
Mitotic Spindle 0.002583 0.003480 0.002701 0.024723 0.003732 0.009573 0.001985 0.024157 0.003081 0.006987 0.002466 0.024191
None 0.008164 0.015679 0.012581 0.004067 0.010251 0.010055 0.011853 0.012955 0.009068 0.012442 0.012342 0.012424
Nuclear Periphery 0.000170 0.000084 0.000417 0.000999 0.000197 0.000119 0.000506 0.000489 0.000182 0.000104 0.000446 0.000519
Nuclear Periphery Foci 0.001885 0.001664 0.000872 0.004093 0.004077 0.001361 0.002225 0.001261 0.002835 0.001490 0.001316 0.001431
Nucleolus 0.000416 0.000222 0.000112 0.000174 0.000211 0.000373 0.000134 0.000510 0.000327 0.000309 0.000119 0.000490
Nucleus 0.088124 0.130527* 0.559689* 0.120523 0.099037 0.099848 0.458566* 0.181051* 0.092851 0.112870 0.526495* 0.177431*
Peroxisomes 0.000478 0.000182 0.000017 0.012882 0.000240 0.000666 0.000118 0.000762 0.000375 0.000461 0.000050 0.001486
Vacuole 0.031363 0.010961 0.009064 0.219308* 0.021192 0.013342 0.026390 0.043665 0.026957 0.012331 0.014752 0.054169
Vacuole Periphery 0.000409 0.000461 0.000055 0.001119 0.000210 0.000938 0.000074 0.002581 0.000322 0.000736 0.000061 0.002493

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.64 -8.24 -1.55 -1.52 -0.87 -1.02 -6.29 -2.60 -0.57 3.66 -1.17 -10.13 -3.15 -1.64 2.45
Bud Neck 4.59 -4.86 -2.49 -3.85 -0.10 -2.09 -9.55 -5.57 -3.41 5.13 0.98 -10.03 -4.39 -4.79 3.64
Bud Site 0.10 3.43 -1.65 -1.66 -1.96 -1.45 -2.39 -4.58 -3.87 -1.76 -0.69 0.23 -4.54 -4.23 -4.54
Cell Periphery -0.36 -10.44 -3.88 -3.68 7.41 7.06 -9.90 2.77 -7.24 10.40 4.59 -14.07 -0.23 -5.67 14.10
Cytoplasm -0.53 45.37 13.59 13.71 -1.21 -2.63 33.69 27.90 32.00 -14.92 -2.79 56.75 32.06 34.54 -22.08
Cytoplasmic Foci 14.90 18.58 10.72 -0.10 -2.43 8.64 9.12 15.33 11.30 4.97 16.84 20.80 24.39 11.49 3.99
Eisosomes 8.78 5.90 -5.31 -7.80 -7.10 7.28 0.35 0.17 -11.97 -0.27 11.05 5.38 -1.04 -15.77 -7.77
Endoplasmic Reticulum 4.00 3.62 -2.46 -4.95 -4.69 6.22 3.47 3.27 -5.78 -0.59 7.04 5.08 2.90 -6.08 -3.15
Endosome 5.65 9.34 -1.31 -1.62 -1.71 0.81 7.21 4.25 2.13 -1.35 3.47 12.11 1.97 -0.25 -2.90
Golgi -1.11 1.81 -0.98 -0.66 -1.20 -2.71 -3.64 -1.03 1.63 -0.50 -2.78 0.88 -0.78 1.34 -0.99
Lipid Particles 1.99 -5.74 -2.88 -3.21 -1.30 2.86 -7.19 0.14 -4.21 7.47 3.53 -8.95 -1.97 -5.45 8.14
Mitochondria 2.44 1.12 1.35 -1.38 0.75 -0.64 -1.14 -1.55 -1.33 -1.23 1.96 0.62 -0.36 -1.58 -0.77
Mitotic Spindle -0.76 -2.11 -2.66 -2.54 -2.38 -2.73 0.10 -5.18 -2.58 -6.00 -3.05 -1.55 -6.54 -4.43 -6.06
None -6.56 -1.95 1.56 6.07 2.84 0.47 0.91 -0.36 -1.09 -1.55 -4.05 -0.94 -1.67 2.16 -0.71
Nuclear Periphery 3.27 -13.60 -7.61 -8.07 -2.58 1.44 -12.39 -12.51 -22.18 5.08 2.72 -17.81 -18.64 -24.16 3.34
Nuclear Periphery Foci -0.14 -0.24 -4.19 -4.12 -3.91 4.66 -0.40 5.22 1.50 6.99 4.26 0.28 5.36 1.86 5.81
Nucleolus 1.94 3.16 1.71 -0.60 -2.22 -0.93 -0.21 -1.05 0.30 -1.03 0.19 3.09 0.17 -0.03 -1.42
Nucleus -11.55 -50.36 -2.76 0.41 23.05 -1.50 -28.23 -22.20 -22.28 16.89 -9.08 -55.59 -25.96 -19.87 34.61
Peroxisomes 3.33 5.47 -1.19 -1.38 -1.47 -2.07 2.43 -0.01 1.81 -1.13 -0.52 6.27 0.04 0.39 -2.18
Vacuole 7.95 -2.00 -10.32 -10.89 -10.01 3.38 -9.92 -20.92 -22.64 -1.27 8.64 -8.73 -21.72 -24.46 -11.77
Vacuole Periphery -0.13 5.39 0.81 0.66 -1.75 -1.41 3.10 -0.99 0.22 -1.16 -1.30 6.20 -0.77 -0.01 -1.22
Standard name
Human Ortholog
Description 3-hydroxyanthranilic acid dioxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bna1

Bna1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bna1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available