Standard name | |
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Human Ortholog | |
Description | 3-hydroxyanthranilic acid dioxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 2 | 2 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 3 | 1 | 16 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 4 | 1 | 2 | 0 | 4 | – | 1 | 7 | 3 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 0 |
Cytoplasm | 250 | 225 | 210 | 178 | 203 | 240 | 403 | – | 175 | 202 | 190 | 198 | 420 | 354 | 378 | 116 | 107 | 69 | 238 | 204 | 204 | 176 | 368 | 360 |
Endoplasmic Reticulum | 3 | 2 | 7 | 0 | 0 | 0 | 2 | – | 1 | 3 | 0 | 0 | 2 | 1 | 0 | 8 | 7 | 8 | 0 | 1 | 0 | 0 | 2 | 1 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 1 | 1 | 0 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 |
Mitochondria | 0 | 0 | 0 | 2 | 2 | 29 | 8 | – | 34 | 48 | 55 | 68 | 0 | 0 | 0 | 1 | 6 | 2 | 0 | 1 | 0 | 1 | 1 | 0 |
Nucleus | 6 | 6 | 7 | 4 | 2 | 7 | 10 | – | 13 | 10 | 15 | 14 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 2 | 1 | 2 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 4 | 0 |
Vac/Vac Membrane | 4 | 5 | 6 | 21 | 31 | 28 | 91 | – | 48 | 53 | 45 | 42 | 5 | 2 | 13 | 4 | 1 | 0 | 1 | 6 | 1 | 1 | 3 | 2 |
Unique Cell Count | 258 | 231 | 223 | 198 | 228 | 286 | 483 | 246 | 287 | 268 | 299 | 424 | 357 | 385 | 124 | 111 | 70 | 258 | 230 | 219 | 188 | 416 | 388 | |
Labelled Cell Count | 265 | 239 | 234 | 206 | 240 | 306 | 520 | 278 | 328 | 310 | 333 | 428 | 357 | 392 | 136 | 125 | 80 | 258 | 230 | 219 | 188 | 416 | 388 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.6 | 11.3 | 10.7 | 10.5 | 11.0 | 6.7 | 8.5 | – | 7.0 | 7.0 | 6.7 | 6.4 | 13.2 | 12.2 | 15.2 | 6.0 | 5.7 | 5.3 | 11.4 | 11.9 | 11.9 |
Std Deviation (1e-4) | 4.3 | 4.0 | 2.9 | 2.7 | 2.6 | 1.9 | 2.2 | – | 2.0 | 2.0 | 2.0 | 2.1 | 3.6 | 3.7 | 4.4 | 2.8 | 2.0 | 2.1 | 3.5 | 3.4 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.04 | -0.67 | -0.34 | – | -0.61 | -0.61 | -0.67 | -0.73 | 0.31 | 0.19 | 0.5 | -0.84 | -0.9 | -1.02 | 0.09 | 0.15 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.6 | -2.0 | -3.6 | -3.9 | – | -6.5 | -6.8 | -6.6 | -7.6 | 3.7 | 3.6 | 2.7 | -0.2 | 0.9 | 1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.9 | 0 | – | 5.8 | 6.4 | 7.2 | 7.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.7 | 0 | -0.5 | -0.9 | – | 1.1 | 0.2 | 1.3 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.3 | 4.2 | 3.2 | 5.8 | – | 5.7 | 5.5 | 5.1 | 4.4 | 0 | 0 | 0.5 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.9712 | 13.3388 | 11.7506 | 10.5283 | 11.1959 | 12.2478 | 13.4888 | 16.7863 | 15.738 | 13.4615 | 13.4406 | 14.652 | 11.6908 | 17.3294 | 14.1081 | 15.3471 | 11.3472 | 14.2414 |
Actin | 0.0026 | 0.0005 | 0.001 | 0.0106 | 0.0005 | 0.0036 | 0.004 | 0.0015 | 0.0015 | 0.0027 | 0.0005 | 0.0004 | 0.0239 | 0.001 | 0.0153 | 0.0313 | 0.0598 | 0.005 |
Bud | 0.0006 | 0.0004 | 0.0014 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.002 | 0.0022 | 0.0052 | 0.0007 | 0.0003 | 0.0025 | 0.0013 | 0.003 | 0.0021 | 0.0007 | 0.0005 |
Bud Neck | 0.0022 | 0.0004 | 0.0006 | 0.0012 | 0.0008 | 0.0016 | 0.0005 | 0.0009 | 0.0004 | 0.0007 | 0.0009 | 0.0012 | 0.0007 | 0.0002 | 0.0011 | 0.0005 | 0.0022 | 0.0013 |
Bud Periphery | 0.0004 | 0.0001 | 0.0006 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0005 | 0.0022 | 0.0039 | 0.0002 | 0.0001 | 0.0012 | 0.0007 | 0.001 | 0.0027 | 0.001 | 0.0002 |
Bud Site | 0.002 | 0.0007 | 0.0019 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0.0034 | 0.0006 | 0.0016 | 0.0002 | 0.0001 | 0.0021 | 0.0011 | 0.0021 | 0.0007 | 0.0023 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 |
Cytoplasm | 0.9035 | 0.9599 | 0.9369 | 0.8728 | 0.9349 | 0.8932 | 0.9128 | 0.9393 | 0.922 | 0.9277 | 0.9405 | 0.9279 | 0.8235 | 0.9696 | 0.8951 | 0.8361 | 0.7861 | 0.9265 |
Cytoplasmic Foci | 0.007 | 0.0012 | 0.0019 | 0.0048 | 0.0057 | 0.0055 | 0.0051 | 0.0058 | 0.0013 | 0.0021 | 0.0018 | 0.002 | 0.0255 | 0.0065 | 0.0102 | 0.015 | 0.0127 | 0.0071 |
Eisosomes | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0001 | 0.0006 | 0.0009 | 0 |
Endoplasmic Reticulum | 0.0037 | 0.0021 | 0.0016 | 0.0023 | 0.0028 | 0.0025 | 0.003 | 0.001 | 0.0018 | 0.0042 | 0.003 | 0.0026 | 0.0069 | 0.0005 | 0.0035 | 0.0044 | 0.007 | 0.0023 |
Endosome | 0.0062 | 0.0006 | 0.001 | 0.0133 | 0.0029 | 0.0031 | 0.0033 | 0.0004 | 0.0007 | 0.0018 | 0.0013 | 0.0016 | 0.0164 | 0.0003 | 0.0041 | 0.0112 | 0.0234 | 0.0053 |
Golgi | 0.0014 | 0 | 0 | 0.0075 | 0.0001 | 0.0007 | 0.0007 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.005 | 0.0001 | 0.0026 | 0.0071 | 0.0099 | 0.0029 |
Lipid Particles | 0.0012 | 0 | 0 | 0.0135 | 0.0001 | 0.001 | 0.0024 | 0 | 0 | 0.0001 | 0 | 0 | 0.006 | 0 | 0.0033 | 0.0035 | 0.017 | 0.0007 |
Mitochondria | 0.0008 | 0.0002 | 0.0002 | 0.0038 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0066 | 0.0002 | 0.0098 | 0.0165 | 0.0073 | 0.0027 |
None | 0.015 | 0.0043 | 0.0099 | 0.0051 | 0.0021 | 0.0034 | 0.0065 | 0.0059 | 0.0017 | 0.0018 | 0.0015 | 0.0014 | 0.0174 | 0.002 | 0.0024 | 0.0057 | 0.0065 | 0.0027 |
Nuclear Periphery | 0.0077 | 0.0026 | 0.0035 | 0.0056 | 0.0057 | 0.0073 | 0.0064 | 0.0027 | 0.0044 | 0.0056 | 0.0054 | 0.0054 | 0.0162 | 0.0011 | 0.0036 | 0.0077 | 0.0149 | 0.0035 |
Nucleolus | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0.001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 |
Nucleus | 0.0391 | 0.0257 | 0.037 | 0.0517 | 0.0383 | 0.0694 | 0.0492 | 0.0323 | 0.0587 | 0.0373 | 0.041 | 0.0539 | 0.0237 | 0.0125 | 0.0262 | 0.0394 | 0.0265 | 0.0359 |
Peroxisomes | 0.0004 | 0 | 0.0001 | 0.0023 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0103 | 0.0009 | 0.0125 | 0.0023 | 0.0126 | 0.0009 |
Punctate Nuclear | 0.0029 | 0.0005 | 0.001 | 0.002 | 0.0033 | 0.0059 | 0.0021 | 0.0024 | 0.001 | 0.0014 | 0.0013 | 0.0014 | 0.0063 | 0.0015 | 0.0027 | 0.0083 | 0.0061 | 0.0015 |
Vacuole | 0.0022 | 0.0006 | 0.001 | 0.0013 | 0.0014 | 0.0011 | 0.0014 | 0.001 | 0.0009 | 0.0027 | 0.001 | 0.0011 | 0.0022 | 0.0002 | 0.0008 | 0.0038 | 0.0015 | 0.0006 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0018 | 0.0001 | 0.0003 | 0.0009 | 0.0009 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 59.9227 | 40.6372 | 31.5252 | 28.0315 | 22.7719 | 68.1502 | 47.1581 | 49.1609 | 43.7563 | 42.5138 |
Translational Efficiency | 1.6144 | 1.4005 | 1.1547 | 1.6544 | 2.4382 | 1.8018 | 1.6566 | 1.2821 | 1.6957 | 1.4337 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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