Standard name
Human Ortholog
Description Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; plays a role in determining prion variants
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.97 0.95 0.83 0.75 0.75 0.72 0.64 0.57 0.59 1.0 0.99 0.98 0.89 0.94 0.94 0.98 0.96 0.97 0.97 0.95 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.07 0.24 0.28 0.24 0.29 0.41 0.48 0.44 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.09 0.08 0.07 0.14 0.13 0.11 0 0 0 0.14 0.09 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 1 0 0 0 1 4 14 6 8 16 13 5 0 0 0 0 1 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 11 4 2 7 13 8 11 10 6 13 1 2 3 12 15 7 0 0 0 0 0 0
Cytoplasm 187 383 496 398 486 508 626 538 378 474 313 402 482 414 485 563 682 466 182 370 476 330 324 364
Endoplasmic Reticulum 0 5 1 2 0 4 8 10 13 7 6 11 0 1 3 15 17 12 0 0 6 0 1 0
Endosome 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 1 1 2 0 0 0 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 1
Mitochondria 2 2 3 25 34 149 236 175 153 301 262 298 0 0 0 4 2 0 0 2 1 1 0 2
Nucleus 0 1 0 0 2 5 6 12 6 7 10 15 2 0 1 11 7 11 0 4 0 2 3 0
Nuclear Periphery 0 0 0 1 0 3 1 5 3 4 0 8 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 1 0 4 12 12 27 79 59 35 106 69 77 0 11 12 91 66 32 0 3 1 3 1 1
Unique Cell Count 189 388 502 412 511 609 835 719 526 735 549 678 482 418 495 635 728 495 186 387 491 342 340 379
Labelled Cell Count 191 393 515 442 537 710 983 813 607 927 680 829 485 429 504 698 791 532 186 387 491 342 340 379


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 7.8 7.5 6.8 6.7 6.0 5.7 5.7 5.6 5.4 5.2 5.1 9.1 8.7 8.4 11.9 11.3 10.5 9.4 9.4 10.3
Std Deviation (1e-4) 1.0 1.1 1.0 0.9 1.2 1.1 1.0 1.1 0.9 1.0 1.2 1.1 1.2 1.1 1.1 2.2 1.9 2.3 1.4 1.4 1.4
Intensity Change (Log2) – – – -0.14 -0.16 -0.31 -0.38 -0.38 -0.41 -0.47 -0.53 -0.54 0.28 0.23 0.17 0.67 0.59 0.49 0.33 0.33 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2.9 0 0 3.3 3.5 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -2.5 -1.4 -0.8 -1.5 -0.1 -1.1 -1.4 -0.3 -2.8 -2.2 -2.1 -0.4 -0.2 -0.9
Cytoplasm -2.3 -3.4 -8.7 -11.5 -11.4 -12.1 -14.4 -16.1 -15.7 2.4 0.3 -1.0 -6.7 -4.4 -4.0
Endoplasmic Reticulum 0 0 0 0 0 3.1 0 0 2.4 0 0 0 3.1 3.1 3.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.8 5.1 11.5 12.8 11.6 12.7 16.2 17.6 16.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 3.4 0 0 0 0 0 3.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.4 2.0 3.7 6.4 5.8 4.9 8.3 7.5 7.1 0 2.2 2.0 8.2 6.2 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5865 5.0619 4.8276 4.844 4.2415 4.6228 3.2635 4.6797 4.4369 3.6635 3.514 4.2663 2.8416 4.1891 3.7322 3.4262 3.3395 3.3574
Actin 0.0325 0.0054 0.0181 0.0519 0.003 0.0047 0.0236 0.0002 0.0006 0.0651 0.0001 0.0083 0.0128 0.0011 0.0015 0.0002 0.0038 0.0027
Bud 0.0006 0.0037 0.0017 0.0004 0.0004 0.0003 0.0009 0.0002 0.0002 0.0018 0.0001 0.0003 0.0007 0.0004 0.001 0.0001 0.0005 0.0002
Bud Neck 0.0023 0.0002 0.0026 0.0009 0.0005 0.0009 0.002 0.0001 0.0003 0.0007 0.0003 0.0031 0.0008 0.0003 0.0004 0.0002 0.001 0.0005
Bud Periphery 0.0015 0.002 0.0019 0.0005 0.0004 0.0002 0.0009 0 0.0001 0.0021 0 0.0003 0.0009 0.0003 0.0011 0 0.0006 0.0005
Bud Site 0.0056 0.0043 0.0094 0.0012 0.0004 0.0004 0.0061 0.0004 0.0004 0.0091 0.0001 0.0007 0.0033 0.0058 0.0018 0 0.0017 0.0001
Cell Periphery 0.001 0.0003 0.0004 0.0006 0 0.0001 0.0002 0 0 0.0002 0 0.0001 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.768 0.9348 0.8551 0.8894 0.7803 0.8979 0.7887 0.9528 0.9393 0.8291 0.9797 0.884 0.8186 0.9577 0.9129 0.9872 0.6625 0.9474
Cytoplasmic Foci 0.037 0.0062 0.0097 0.0048 0.0453 0.0113 0.0191 0.0045 0.0053 0.0201 0.0039 0.0262 0.0158 0.0052 0.0133 0.0017 0.0511 0.0046
Eisosomes 0.0011 0.0001 0.0001 0.0004 0 0 0.0002 0 0 0.0003 0 0.0001 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.006 0.0015 0.0039 0.0035 0.0032 0.0019 0.0063 0.0018 0.0017 0.0037 0.0017 0.0028 0.0048 0.0011 0.0025 0.0022 0.0021 0.0015
Endosome 0.0149 0.0027 0.0188 0.0031 0.0705 0.006 0.0302 0.0052 0.0082 0.0252 0.0031 0.0187 0.0171 0.0035 0.0176 0.0006 0.1356 0.0072
Golgi 0.0069 0.0011 0.006 0.0022 0.0242 0.0041 0.0061 0.0002 0.0007 0.0055 0.0001 0.0066 0.0077 0.0021 0.0109 0 0.0649 0.0033
Lipid Particles 0.0125 0.0006 0.0022 0.0016 0.0053 0.008 0.0052 0 0.0007 0.003 0 0.0058 0.0106 0.0001 0.0012 0 0.0207 0.0006
Mitochondria 0.0114 0.0013 0.0091 0.0008 0.0036 0.0011 0.0104 0.0002 0.0025 0.0073 0.0001 0.0042 0.0099 0.0015 0.0079 0.0001 0.0234 0.0019
None 0.0657 0.0302 0.0457 0.0134 0.039 0.0466 0.0527 0.0293 0.0255 0.0119 0.0048 0.0099 0.0766 0.0175 0.0125 0.0031 0.002 0.0107
Nuclear Periphery 0.0023 0.0008 0.0041 0.0031 0.0035 0.0023 0.0123 0.0007 0.0025 0.0019 0.001 0.0053 0.0019 0.0005 0.0012 0.0007 0.0022 0.0096
Nucleolus 0.0004 0.0001 0.0006 0.0007 0.0002 0.0005 0.0008 0 0.0005 0.0006 0 0.0005 0.0003 0 0.0001 0 0.0003 0.0001
Nucleus 0.0019 0.0017 0.0031 0.013 0.0035 0.005 0.0154 0.0024 0.0044 0.0038 0.0035 0.0046 0.0037 0.0015 0.0036 0.0031 0.0017 0.0037
Peroxisomes 0.0246 0.0005 0.0027 0.0004 0.0085 0.0024 0.0045 0.0001 0.0003 0.0016 0 0.0075 0.0058 0.0003 0.0079 0 0.0168 0.0015
Punctate Nuclear 0.0016 0.0019 0.003 0.001 0.0054 0.0056 0.0093 0.0003 0.0042 0.0015 0.0002 0.009 0.004 0.0004 0.0004 0.0001 0.0018 0.0004
Vacuole 0.0019 0.0006 0.0014 0.0066 0.0019 0.0005 0.0042 0.0011 0.0016 0.0044 0.0011 0.0012 0.0035 0.0006 0.0015 0.0004 0.0036 0.0013
Vacuole Periphery 0.0005 0.0001 0.0005 0.0004 0.0009 0.0002 0.0011 0.0002 0.0008 0.001 0.0002 0.0008 0.001 0.0002 0.0007 0.0001 0.0035 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.3377 14.1865 23.5309 26.9633 30.8635 21.4588 23.6253 26.1961 25.2675 27.7927
Translational Efficiency 1.8553 3.115 1.6708 1.5829 1.3571 1.7121 1.7504 1.1628 1.3352 1.6387

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; plays a role in determining prion variants
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cpr7

Cpr7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cpr7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available