Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 17 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 1 | 5 | 0 | 0 | 0 | 5 | 8 | 2 | 7 | 3 | 5 | 0 | 2 | 4 |
Bud | 5 | 2 | 4 | 2 | 5 | 3 | 4 | 10 | 6 | 18 | 12 | 7 | 0 | 0 | 1 | 4 | 4 | 2 | 2 | 3 | 0 | 3 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 1 | 3 | 2 | 3 | 2 | 2 | 2 | 3 | 3 | 1 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 325 | 199 | 166 | 181 | 225 | 228 | 302 | 215 | 195 | 249 | 169 | 157 | 115 | 26 | 74 | 563 | 394 | 312 | 92 | 58 | 31 | 15 | 23 | 5 |
Endoplasmic Reticulum | 36 | 9 | 3 | 5 | 2 | 3 | 3 | 5 | 11 | 4 | 3 | 11 | 16 | 0 | 8 | 44 | 40 | 31 | 8 | 7 | 13 | 2 | 8 | 7 |
Endosome | 81 | 9 | 93 | 48 | 81 | 10 | 37 | 50 | 1 | 11 | 3 | 4 | 3 | 1 | 1 | 51 | 62 | 75 | 211 | 80 | 132 | 43 | 45 | 49 |
Golgi | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 26 | 11 | 16 | 2 | 10 | 16 |
Mitochondria | 12 | 19 | 18 | 42 | 54 | 174 | 240 | 235 | 227 | 301 | 240 | 264 | 0 | 0 | 0 | 50 | 25 | 33 | 70 | 25 | 53 | 31 | 26 | 22 |
Nucleus | 1 | 0 | 0 | 0 | 1 | 3 | 0 | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 1 | 3 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 5 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 8 | 0 | 12 | 3 | 2 | 1 | 1 | 3 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 14 | 6 | 13 | 7 | 1 | 2 | 1 | 1 | 0 |
Vac/Vac Membrane | 109 | 17 | 48 | 23 | 58 | 25 | 42 | 29 | 9 | 19 | 15 | 16 | 6 | 3 | 14 | 38 | 51 | 65 | 195 | 94 | 83 | 41 | 84 | 54 |
Unique Cell Count | 524 | 245 | 306 | 260 | 369 | 346 | 503 | 442 | 355 | 464 | 347 | 372 | 136 | 29 | 95 | 683 | 497 | 444 | 634 | 296 | 350 | 147 | 206 | 167 |
Labelled Cell Count | 597 | 258 | 352 | 306 | 434 | 449 | 637 | 557 | 454 | 612 | 449 | 472 | 141 | 30 | 100 | 774 | 594 | 541 | 634 | 296 | 350 | 147 | 206 | 167 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 6.4 | 5.4 | 5.2 | 5.5 | 4.6 | 4.6 | 4.9 | 4.5 | 4.4 | 4.4 | 4.6 | 6.7 | 6.9 | 7.1 | 5.6 | 6.1 | 6.8 | 6.6 | 7.3 | 8.4 |
Std Deviation (1e-4) | 0.6 | 0.9 | 1.4 | 1.3 | 1.9 | 1.2 | 1.2 | 1.4 | 1.2 | 1.1 | 1.1 | 1.0 | 0.8 | 0.6 | 1.2 | 1.1 | 1.2 | 1.5 | 1.3 | 1.4 | 2.5 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.03 | -0.23 | -0.22 | -0.13 | -0.25 | -0.29 | -0.28 | -0.22 | 0.32 | 0.36 | 0.4 | 0.05 | 0.18 | 0.34 | 0.3 | 0.43 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.7 | 1.8 | 3.0 | 1.6 | -1.5 | 0.2 | -0.2 | -1.4 | -3.1 | 6.1 | 3.7 | 4.1 | 9.3 | 7.5 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.2 | 0 | 0 | 3.7 | 4.3 | 3.9 |
Endosome | -3.3 | -2.5 | 0 | 0 | -6.5 | 0 | 0 | 0 | 0 | -6.6 | -3.1 | -5.9 | -9.4 | -6.2 | -4.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.0 | 3.7 | 0 | 0 | 13.4 | 0 | 0 | 0 | 0 | -2.9 | 0 | 0 | 0.8 | -0.5 | 0.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -2.0 | -3.1 | 0 | 0 | -3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.7 | -2.5 | -0.7 |
Vacuole | -2.4 | 0 | -3.4 | -3.2 | -4.0 | -6.0 | -5.6 | -4.9 | -5.0 | -3.3 | 0 | -0.2 | -5.2 | -2.3 | -0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9696 | 1.6954 | 1.4247 | 1.2315 | 0.7848 | 1.0452 | 3.3878 | 3.1157 | 3.2243 | 3.7484 | 3.0631 | 3.4436 | 2.4803 | 2.3515 | 2.8902 | 2.8989 | 2.4388 | 2.8594 |
Actin | 0.0018 | 0.0003 | 0.0006 | 0.0002 | 0.0107 | 0.0003 | 0.0262 | 0.0005 | 0.0147 | 0.0388 | 0.0264 | 0.0018 | 0.0004 | 0.0001 | 0.0003 | 0.0005 | 0 | 0.0001 |
Bud | 0.0011 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0015 | 0.0005 | 0.0006 | 0.0017 | 0.0002 | 0.0006 | 0.0002 | 0.0009 | 0.0009 | 0.0002 | 0.0002 | 0.0002 |
Bud Neck | 0.0007 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0007 | 0.0051 | 0.0002 | 0.0012 | 0.0006 | 0.0003 | 0.002 | 0.0002 | 0.0001 | 0.0021 | 0.0002 | 0.0002 | 0.0004 |
Bud Periphery | 0.0007 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0027 | 0.0006 | 0.0007 | 0.0026 | 0.0002 | 0.0006 | 0.0001 | 0.0004 | 0.001 | 0.0001 | 0.0001 | 0.0003 |
Bud Site | 0.0061 | 0.0024 | 0.0005 | 0.0004 | 0.0014 | 0.0005 | 0.0095 | 0.0014 | 0.0038 | 0.0021 | 0.0009 | 0.0012 | 0.0011 | 0.0004 | 0.0007 | 0.0003 | 0.0003 | 0.0001 |
Cell Periphery | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0002 |
Cytoplasm | 0.0684 | 0.2755 | 0.2311 | 0.1789 | 0.2442 | 0.2787 | 0.1369 | 0.3046 | 0.2282 | 0.107 | 0.127 | 0.2416 | 0.0758 | 0.273 | 0.163 | 0.266 | 0.1458 | 0.2979 |
Cytoplasmic Foci | 0.0669 | 0.0717 | 0.0848 | 0.1382 | 0.1063 | 0.1305 | 0.1276 | 0.1266 | 0.1357 | 0.0701 | 0.1063 | 0.1311 | 0.2129 | 0.0713 | 0.097 | 0.0773 | 0.0704 | 0.075 |
Eisosomes | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0055 | 0.0092 | 0.015 | 0.0033 | 0.0091 | 0.0065 | 0.0079 | 0.0052 | 0.0052 | 0.0056 | 0.0026 | 0.0026 | 0.0073 | 0.0296 | 0.0077 | 0.0123 | 0.0022 | 0.0092 |
Endosome | 0.5009 | 0.4239 | 0.4439 | 0.5006 | 0.4478 | 0.4624 | 0.4046 | 0.3615 | 0.3758 | 0.4463 | 0.53 | 0.4126 | 0.5737 | 0.3053 | 0.3542 | 0.4338 | 0.5046 | 0.4369 |
Golgi | 0.0195 | 0.0111 | 0.0168 | 0.0214 | 0.0265 | 0.0227 | 0.0305 | 0.0183 | 0.0201 | 0.0134 | 0.016 | 0.0218 | 0.0598 | 0.0125 | 0.0492 | 0.0154 | 0.0488 | 0.0208 |
Lipid Particles | 0.0247 | 0.004 | 0.0087 | 0.0172 | 0.0292 | 0.0048 | 0.0161 | 0.0066 | 0.0049 | 0.0476 | 0.0185 | 0.0101 | 0.0104 | 0.0154 | 0.0047 | 0.0074 | 0.0025 | 0.0013 |
Mitochondria | 0.0127 | 0.0088 | 0.0136 | 0.0028 | 0.004 | 0.0081 | 0.0194 | 0.0049 | 0.0068 | 0.0075 | 0.0028 | 0.0105 | 0.0047 | 0.0092 | 0.0215 | 0.0034 | 0.0021 | 0.0181 |
None | 0.0133 | 0.0723 | 0.1195 | 0.0177 | 0.0127 | 0.0308 | 0.1108 | 0.1192 | 0.1381 | 0.143 | 0.0484 | 0.1034 | 0.0214 | 0.1264 | 0.1711 | 0.1213 | 0.19 | 0.0989 |
Nuclear Periphery | 0.0469 | 0.0047 | 0.0042 | 0.003 | 0.0071 | 0.0026 | 0.0075 | 0.0018 | 0.0018 | 0.0018 | 0.0017 | 0.0072 | 0.002 | 0.0057 | 0.0023 | 0.0057 | 0.0016 | 0.0018 |
Nucleolus | 0.0065 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0 |
Nucleus | 0.122 | 0.0021 | 0.0015 | 0.0015 | 0.0031 | 0.002 | 0.0023 | 0.0014 | 0.0012 | 0.0021 | 0.0006 | 0.0013 | 0.0006 | 0.0011 | 0.0014 | 0.0018 | 0.0012 | 0.0008 |
Peroxisomes | 0.0041 | 0.0008 | 0.0024 | 0.0046 | 0.0026 | 0.0018 | 0.0159 | 0.0013 | 0.0152 | 0.0044 | 0.0597 | 0.0135 | 0.002 | 0.0023 | 0.0045 | 0.0013 | 0.0011 | 0.0007 |
Punctate Nuclear | 0.0577 | 0.0008 | 0.0011 | 0.0011 | 0.0012 | 0.0016 | 0.004 | 0.0004 | 0.0014 | 0.0007 | 0.0039 | 0.0008 | 0.0009 | 0.0002 | 0.0005 | 0.0013 | 0.0005 | 0.0002 |
Vacuole | 0.0312 | 0.0928 | 0.0407 | 0.0863 | 0.0768 | 0.0347 | 0.0598 | 0.0366 | 0.0361 | 0.0809 | 0.0421 | 0.0261 | 0.02 | 0.1062 | 0.0984 | 0.0422 | 0.0223 | 0.0247 |
Vacuole Periphery | 0.0089 | 0.0176 | 0.0142 | 0.0218 | 0.0161 | 0.0104 | 0.0093 | 0.0076 | 0.0076 | 0.0225 | 0.0121 | 0.0109 | 0.006 | 0.0397 | 0.0186 | 0.0091 | 0.0061 | 0.0125 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.3232 | 7.9898 | 8.6237 | 13.018 | 8.0766 | 10.8276 | 11.3221 | 13.0528 | 13.7131 | 14.7592 |
Translational Efficiency | 0.3201 | 0.3946 | 0.5157 | 0.4068 | 0.4912 | 0.3823 | 0.4431 | 0.3047 | 0.3488 | 0.4241 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
298 | 1344 | 674 | 1185 | 1078 | 1363 | 226 | 1719 | 1376 | 2707 | 900 | 2904 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 860.93 | 697.75 | 1030.06 | 912.88 | 628.50 | 817.12 | 981.83 | 913.04 | 678.84 | 757.85 | 1017.95 | 912.97 |
Standard Deviation | 130.24 | 95.59 | 124.84 | 155.95 | 82.75 | 102.18 | 111.50 | 123.50 | 134.92 | 115.57 | 123.41 | 137.67 |
Intensity Change Log 2 | — | -0.303186 | 0.258761 | 0.084529 | — | 0.378635 | 0.643561 | 0.538765 | — | 0.024434 | 0.433791 | 0.293863 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002394 | 0.000302 | 0.000488 | 0.003289 | 0.001353 | 0.000574 | 0.001013 | 0.001505 | 0.001578 | 0.000439 | 0.000620 | 0.002233 |
Bud Neck | 0.009688 | 0.008539 | 0.002152 | 0.004628 | 0.014780 | 0.009363 | 0.008135 | 0.005749 | 0.013677 | 0.008954 | 0.003654 | 0.005292 |
Bud Site | 0.037675 | 0.012968 | 0.017036 | 0.046757 | 0.011481 | 0.010867 | 0.016332 | 0.033351 | 0.017154 | 0.011910 | 0.016859 | 0.038822 |
Cell Periphery | 0.000161 | 0.000062 | 0.000052 | 0.000086 | 0.000465 | 0.000098 | 0.000065 | 0.000050 | 0.000399 | 0.000080 | 0.000055 | 0.000065 |
Cytoplasm | 0.069354 | 0.120449* | 0.036934 | 0.116235* | 0.097315 | 0.092079 | 0.064629 | 0.133572* | 0.091259 | 0.106165* | 0.043888 | 0.126497* |
Cytoplasmic Foci | 0.232013* | 0.422343* | 0.259983* | 0.195162* | 0.334916* | 0.375527* | 0.170793 | 0.259675* | 0.312631* | 0.398771* | 0.237586* | 0.233350* |
Eisosomes | 0.000070 | 0.000091 | 0.000017 | 0.000027 | 0.001134 | 0.000170 | 0.000050 | 0.000029 | 0.000904 | 0.000130 | 0.000025 | 0.000028 |
Endoplasmic Reticulum | 0.001478 | 0.001570 | 0.001851 | 0.000992 | 0.002336 | 0.002125 | 0.003108 | 0.001161 | 0.002150 | 0.001850 | 0.002166 | 0.001092 |
Endosome | 0.328530* | 0.197960 | 0.236227* | 0.190189 | 0.097331 | 0.204124* | 0.243880* | 0.211043* | 0.147402 | 0.201064* | 0.238149* | 0.202533* |
Golgi | 0.042526 | 0.058356 | 0.095916 | 0.045162 | 0.210071* | 0.097728 | 0.010484 | 0.041769 | 0.173786* | 0.078180 | 0.074463 | 0.043154 |
Lipid Particles | 0.004384 | 0.017818 | 0.003245 | 0.003089 | 0.028970 | 0.015827 | 0.008110 | 0.003259 | 0.023645 | 0.016816 | 0.004467 | 0.003190 |
Mitochondria | 0.015092 | 0.017964 | 0.001383 | 0.002894 | 0.142002* | 0.035021 | 0.000917 | 0.004766 | 0.114517* | 0.026552 | 0.001266 | 0.004002 |
Mitotic Spindle | 0.010727 | 0.011421 | 0.025304 | 0.014491 | 0.000770 | 0.005580 | 0.015487 | 0.014160 | 0.002926 | 0.008480 | 0.022839 | 0.014295 |
None | 0.000055 | 0.001252 | 0.000075 | 0.000084 | 0.001364 | 0.000238 | 0.000302 | 0.000107 | 0.001081 | 0.000741 | 0.000132 | 0.000098 |
Nuclear Periphery | 0.000220 | 0.000547 | 0.001049 | 0.000267 | 0.000076 | 0.000155 | 0.001866 | 0.000208 | 0.000107 | 0.000350 | 0.001254 | 0.000232 |
Nuclear Periphery Foci | 0.000684 | 0.001873 | 0.004181 | 0.001875 | 0.000162 | 0.000259 | 0.008483 | 0.001147 | 0.000275 | 0.001060 | 0.005262 | 0.001444 |
Nucleolus | 0.000400 | 0.002389 | 0.000583 | 0.000415 | 0.000526 | 0.000402 | 0.003442 | 0.000306 | 0.000498 | 0.001389 | 0.001301 | 0.000350 |
Nucleus | 0.002627 | 0.006652 | 0.001544 | 0.001762 | 0.005021 | 0.003361 | 0.022621 | 0.002406 | 0.004503 | 0.004995 | 0.006837 | 0.002143 |
Peroxisomes | 0.025614 | 0.016212 | 0.006015 | 0.012053 | 0.021222 | 0.009753 | 0.020563 | 0.017309 | 0.022173 | 0.012960 | 0.009668 | 0.015164 |
Vacuole | 0.202870* | 0.086673 | 0.244600* | 0.330916* | 0.013441 | 0.107173 | 0.382502* | 0.248965* | 0.054466 | 0.096995 | 0.279229* | 0.282406* |
Vacuole Periphery | 0.013440 | 0.014559 | 0.061366 | 0.029625 | 0.015264 | 0.029577 | 0.017219 | 0.019463 | 0.014869 | 0.022121 | 0.050280 | 0.023609 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.80 | 2.52 | -0.57 | -2.97 | -2.75 | 2.32 | 0.67 | -0.26 | -2.02 | -0.77 | 4.12 | 3.51 | -1.07 | -3.69 | -3.27 |
Bud Neck | 0.57 | 4.03 | 2.56 | 6.07 | -7.17 | 3.48 | 3.57 | 6.14 | 4.54 | 2.57 | 3.79 | 8.15 | 6.85 | 7.09 | -4.38 |
Bud Site | 4.63 | 3.67 | -1.36 | -10.84 | -8.40 | 0.47 | -2.99 | -10.96 | -11.36 | -6.30 | 3.46 | -0.08 | -10.10 | -15.55 | -9.90 |
Cell Periphery | 2.32 | 2.55 | 1.79 | -2.30 | -3.46 | 8.31 | 9.00 | 9.53 | 6.46 | 2.73 | 8.92 | 9.62 | 9.42 | 3.19 | -1.34 |
Cytoplasm | -4.89 | 3.74 | -5.66 | -1.26 | -13.47 | 0.83 | 1.56 | -6.54 | -7.80 | -5.81 | -2.80 | 7.67 | -8.17 | -6.27 | -16.53 |
Cytoplasmic Foci | -15.06 | -2.00 | 3.84 | 30.51 | 7.92 | -5.20 | 15.24 | 11.22 | 16.83 | -8.19 | -13.18 | 9.42 | 14.10 | 32.14 | 1.31 |
Eisosomes | -1.23 | 3.67 | 2.99 | 6.18 | -3.18 | 5.55 | 6.24 | 6.38 | 9.65 | 4.08 | 5.66 | 6.44 | 6.43 | 11.40 | -0.62 |
Endoplasmic Reticulum | -0.24 | -0.92 | 1.08 | 2.40 | 3.21 | 0.56 | -1.81 | 3.29 | 3.08 | 4.45 | 1.04 | -0.26 | 3.76 | 3.71 | 4.61 |
Endosome | 7.77 | 5.23 | 9.19 | 3.14 | 7.13 | -18.54 | -8.67 | -18.19 | 0.39 | 1.29 | -9.28 | -10.99 | -7.60 | 2.08 | 5.78 |
Golgi | -2.37 | -6.34 | 0.06 | 4.57 | 8.99 | 14.96 | 29.98 | 25.27 | 13.34 | -10.89 | 15.81 | 14.33 | 23.05 | 13.19 | 7.34 |
Lipid Particles | -7.52 | 0.96 | 1.06 | 9.36 | 0.06 | 5.72 | 8.99 | 13.03 | 10.40 | 3.96 | 3.64 | 11.63 | 12.86 | 13.79 | 2.45 |
Mitochondria | -0.57 | 2.35 | 2.12 | 10.76 | -3.25 | 16.87* | 23.94 | 23.20 | 12.58 | -5.92 | 17.03 | 22.72 | 22.16 | 15.93 | -6.11 |
Mitotic Spindle | 0.27 | -2.30 | -0.67 | -1.68 | 2.41 | -4.31 | -4.79 | -9.00 | -5.03 | 0.55 | -3.85 | -7.46 | -7.74 | -4.41 | 2.70 |
None | -3.14 | -0.99 | -1.33 | 3.07 | -0.37 | 1.35 | 1.20 | 1.52 | 1.27 | 1.45 | 0.48 | 1.44 | 1.51 | 3.28 | 0.98 |
Nuclear Periphery | -1.76 | -2.66 | -1.43 | 1.16 | 2.31 | -3.13 | -5.15 | -4.90 | -2.08 | 4.67 | -2.72 | -4.67 | -5.23 | 0.76 | 3.97 |
Nuclear Periphery Foci | -3.72 | -4.07 | -3.12 | -0.71 | 1.92 | -1.61 | -3.46 | -2.66 | -2.39 | 3.02 | -5.25 | -5.69 | -4.48 | -1.64 | 3.94 |
Nucleolus | -4.80 | -1.04 | -0.75 | 4.65 | 0.79 | 0.61 | -3.76 | 1.09 | 0.63 | 4.18 | -3.56 | -2.70 | 0.81 | 4.71 | 3.53 |
Nucleus | -3.43 | 1.68 | 0.80 | 4.78 | -1.76 | 1.39 | -3.60 | 1.89 | 0.73 | 4.03 | -0.37 | -1.89 | 2.13 | 4.14 | 3.42 |
Peroxisomes | 2.70 | 5.51 | 4.08 | 3.05 | -3.11 | 8.15 | 0.28 | 2.24 | -4.49 | 0.68 | 6.69 | 6.51 | 4.79 | -1.49 | -2.89 |
Vacuole | 8.14 | -2.28 | -8.40 | -28.88 | -8.25 | -22.00 | -22.02 | -39.89 | -20.56 | 7.41 | -8.81 | -24.99 | -37.86 | -34.63 | -1.12 |
Vacuole Periphery | -0.67 | -11.53 | -5.48 | -6.55 | 8.38 | -8.25 | 0.01 | -2.61 | 6.43 | -1.32 | -6.08 | -11.52 | -6.08 | 0.12 | 9.10 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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