Standard name
Human Ortholog
Description DNA replication licensing factor; required for pre-replication complex assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.05 0
Bud 0 0 – 0.05 0 0 0 – 0 0 0 0.05 – – – 0 0 0 0 0 0 0 0.06 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0.05 0 0 0 – 0 0 0 0 – – – 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.57 0.54 – 0.73 0.85 0.86 0.82 – 0.59 0.54 0.5 0.38 – – – 0.64 0.61 0.62 0.47 0.45 0.56 0.39 0.23 0.42
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0.05 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.21 0.26 0.06
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0 0.07 – 0.4 0.36 0.59 0.59 – – – 0 0 0 0 0 0 0 0 0
Nucleus 0.29 0.23 – 0.05 0 0.05 0 – 0.1 0.13 0.13 0.11 – – – 0.14 0.15 0.12 0.4 0.34 0.14 0.05 0.06 0.1
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.05
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.05 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.07 0 0
Vac/Vac Membrane 0.27 0.42 – 0.23 0.18 0.06 0.09 – 0.09 0.09 0 0.13 – – – 0.31 0.28 0.29 0 0.09 0.17 0.1 0.11 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 1 – 0 0 0 0 – – – 1 1 0 0 3 1 0 1 0
Bud 0 1 – 1 0 2 0 – 2 0 0 4 – – – 0 0 0 5 9 0 0 1 1
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 1 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 1 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – 1 0 0 0 – 0 0 1 0 – – – 6 12 12 0 0 0 0 0 0
Cytoplasm 124 150 – 16 29 96 133 – 40 36 54 29 – – – 204 198 159 105 131 30 3 6 13
Endoplasmic Reticulum 0 4 – 0 0 2 2 – 0 0 0 0 – – – 8 11 10 0 1 0 0 0 0
Endosome 5 1 – 1 0 1 1 – 0 0 2 1 – – – 4 3 5 4 3 0 2 7 1
Golgi 0 2 – 0 1 0 0 – 0 0 0 0 – – – 5 2 2 0 2 0 0 1 0
Mitochondria 7 0 – 0 0 4 11 – 27 24 64 45 – – – 6 0 2 1 3 1 0 0 0
Nucleus 62 65 – 1 1 6 7 – 7 9 14 8 – – – 45 50 31 91 100 7 0 1 3
Nuclear Periphery 0 0 – 0 0 1 0 – 2 0 0 0 – – – 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 1 0 0 1 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 4 5 0 0 0 0
Vac/Vac Membrane 58 117 – 5 6 7 15 – 6 6 4 10 – – – 100 92 73 9 26 9 0 3 2
Unique Cell Count 217 278 22 34 112 163 68 67 109 76 321 323 255 227 294 55 10 28 33
Labelled Cell Count 256 340 25 37 119 170 84 75 140 97 379 369 294 227 294 55 10 28 33


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.7 – 7.7 7.5 6.0 6.1 – 4.7 5.0 4.6 4.5 – – – 11.3 11.2 10.6 – – –
Std Deviation (1e-4) 0.8 1.3 – 2.4 1.7 1.4 1.9 – 0.9 1.0 2.1 1.2 – – – 1.9 2.0 1.8 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4094 2.0599 1.7073 1.3226 1.8652 1.8392 1.9923 2.5527 2.2071 2.0984 1.972 2.1971 1.3089 2.3416 2.005 2.1162 1.7475 1.8968
Actin 0.0247 0.0008 0.0028 0.0253 0.0057 0.0008 0.0172 0.0004 0.009 0.0141 0.013 0.0035 0.0339 0.0015 0.0372 0.0411 0.0097 0.006
Bud 0.001 0.0065 0.0066 0.0124 0.0014 0.0006 0.0007 0.0007 0.0015 0.001 0.0005 0.0002 0.0026 0.0069 0.0007 0.0009 0.0031 0.0039
Bud Neck 0.0019 0.0008 0.0018 0.0013 0.0011 0.0044 0.0029 0.0009 0.0049 0.0107 0.0019 0.0028 0.0032 0.0005 0.0007 0.0008 0.0015 0.0032
Bud Periphery 0.0018 0.0014 0.0033 0.0038 0.0023 0.0005 0.0009 0.0004 0.0014 0.0008 0.0004 0.0002 0.0023 0.0012 0.0005 0.001 0.0015 0.0022
Bud Site 0.0036 0.0065 0.0036 0.0109 0.0027 0.0004 0.0029 0.0071 0.0065 0.0016 0.001 0.0003 0.0103 0.0095 0.0018 0.0018 0.0039 0.0014
Cell Periphery 0.0005 0.0002 0.0002 0.0003 0.0002 0 0.0001 0.0002 0.0003 0.0002 0.0001 0 0.0003 0.0001 0.0001 0.0003 0.0002 0.0002
Cytoplasm 0.2513 0.5183 0.6723 0.709 0.5817 0.1505 0.1989 0.4827 0.6328 0.6555 0.6674 0.1425 0.2701 0.4913 0.7451 0.5887 0.7031 0.1823
Cytoplasmic Foci 0.0146 0.0059 0.0228 0.0126 0.0209 0.008 0.017 0.0042 0.0162 0.0261 0.0223 0.0044 0.0396 0.0034 0.0123 0.0735 0.0197 0.0057
Eisosomes 0.0002 0 0.0001 0.0002 0.0001 0 0.0002 0 0.0001 0.0002 0.0001 0 0.0003 0 0.0001 0.0004 0.0001 0
Endoplasmic Reticulum 0.0137 0.0093 0.0095 0.0034 0.0039 0.0022 0.0058 0.0038 0.0052 0.0116 0.0038 0.0017 0.0058 0.006 0.006 0.0077 0.0019 0.002
Endosome 0.0352 0.005 0.0341 0.0094 0.0586 0.0142 0.0275 0.0041 0.0116 0.0224 0.0313 0.0057 0.0282 0.0032 0.0195 0.0263 0.0327 0.014
Golgi 0.0127 0.0011 0.0059 0.004 0.0298 0.0044 0.0031 0.0002 0.0034 0.0057 0.0048 0.0016 0.0108 0.0048 0.0036 0.0267 0.0142 0.0071
Lipid Particles 0.0148 0.0002 0.0114 0.0004 0.0117 0.0021 0.0045 0.0001 0.0048 0.008 0.0049 0.0031 0.0071 0.0002 0.0021 0.0335 0.0185 0.0024
Mitochondria 0.0139 0.0018 0.0096 0.0213 0.0212 0.0016 0.0041 0.0007 0.0062 0.005 0.0019 0.0009 0.0131 0.0008 0.0043 0.0387 0.0104 0.0043
None 0.0767 0.0411 0.0897 0.128 0.0527 0.0311 0.0597 0.0511 0.1328 0.084 0.0976 0.0156 0.0245 0.0208 0.061 0.0799 0.056 0.0292
Nuclear Periphery 0.0322 0.0193 0.0129 0.0051 0.0038 0.0171 0.0288 0.0115 0.0086 0.0106 0.0156 0.0069 0.019 0.0156 0.0092 0.0052 0.0057 0.0079
Nucleolus 0.0027 0.0014 0.001 0.0026 0.0017 0.0031 0.0047 0.0015 0.0016 0.0012 0.001 0.003 0.0036 0.001 0.0002 0.0003 0.0008 0.0061
Nucleus 0.4687 0.3621 0.0809 0.037 0.1579 0.7312 0.5877 0.4131 0.1239 0.1085 0.1073 0.7874 0.4744 0.4248 0.0816 0.0056 0.0863 0.7083
Peroxisomes 0.0075 0.0002 0.0082 0.0004 0.0222 0.0078 0.0058 0.0002 0.0093 0.018 0.0022 0.0025 0.0289 0.0002 0.0039 0.0522 0.0097 0.001
Punctate Nuclear 0.0117 0.0136 0.0067 0.0057 0.0026 0.0161 0.0211 0.013 0.0091 0.0046 0.0179 0.0158 0.0146 0.0053 0.0022 0.0111 0.0144 0.0078
Vacuole 0.0085 0.004 0.0135 0.0059 0.0154 0.0025 0.0048 0.0037 0.009 0.0089 0.004 0.0014 0.0061 0.0026 0.0043 0.003 0.0048 0.004
Vacuole Periphery 0.0023 0.0006 0.003 0.0012 0.0021 0.0013 0.0015 0.0004 0.0016 0.0014 0.001 0.0003 0.0014 0.0004 0.0034 0.0013 0.0016 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.996 47.2808 29.4387 36.9762 30.3463 35.8484 52.751 49.3255 43.2674 42.3404
Translational Efficiency 0.4572 0.5664 0.5265 0.4926 0.5336 0.4865 0.4708 0.3819 0.504 0.3532

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2237 1521 1557 1728 1617 1424 1837 1670 3854 2945 3394 3398

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 715.28 785.11 1037.05 1150.36 788.27 824.13 1044.47 1144.80 745.90 803.98 1041.07 1147.63
Standard Deviation 78.23 110.83 121.24 167.86 100.42 109.53 137.69 161.65 95.30 111.91 130.45 164.86
Intensity Change Log 2 — 0.134387 0.535905 0.685505 — 0.064182 0.406009 0.538334 — 0.098007 0.469265 0.610222

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000049 0.000300 0.000370 0.000538 0.000075 0.000223 0.000240 0.000347 0.000060 0.000263 0.000300 0.000444
Bud Neck 0.005064 0.010086 0.004185 0.004163 0.002500 0.008563 0.004485 0.006785 0.003988 0.009349 0.004347 0.005452
Bud Site 0.002495 0.009623 0.002651 0.023890 0.001276 0.005671 0.003371 0.021488 0.001984 0.007712 0.003041 0.022709
Cell Periphery 0.000152 0.000151 0.000082 0.000199 0.000090 0.000100 0.000105 0.000098 0.000126 0.000126 0.000094 0.000149
Cytoplasm 0.437757* 0.415100* 0.251444* 0.628066* 0.368743* 0.316112* 0.299697* 0.593687* 0.408801* 0.367236* 0.277561* 0.611170*
Cytoplasmic Foci 0.049245 0.047538 0.003830 0.017485 0.037392 0.027744 0.003674 0.019389 0.044272 0.037967 0.003745 0.018421
Eisosomes 0.000043 0.000030 0.000012 0.000010 0.000024 0.000021 0.000013 0.000011 0.000035 0.000026 0.000013 0.000011
Endoplasmic Reticulum 0.000945 0.002657 0.003475 0.001741 0.001255 0.002066 0.002622 0.001300 0.001075 0.002371 0.003013 0.001524
Endosome 0.003103 0.006019 0.000723 0.002410 0.002586 0.003630 0.000786 0.005840 0.002886 0.004864 0.000757 0.004096
Golgi 0.000521 0.001266 0.000071 0.002927 0.000345 0.001037 0.000069 0.001990 0.000447 0.001155 0.000070 0.002466
Lipid Particles 0.002099 0.001434 0.000324 0.000601 0.001296 0.001232 0.000299 0.000461 0.001762 0.001337 0.000311 0.000532
Mitochondria 0.001518 0.002253 0.000750 0.002957 0.000460 0.002728 0.000722 0.003995 0.001074 0.002483 0.000735 0.003467
Mitotic Spindle 0.002441 0.004529 0.007149 0.032506 0.004169 0.010747 0.006246 0.069406 0.003166 0.007536 0.006660 0.050641
None 0.013320 0.010240 0.004955 0.007354 0.007559 0.006451 0.007697 0.007746 0.010903 0.008408 0.006439 0.007547
Nuclear Periphery 0.000560 0.000773 0.001246 0.001171 0.001229 0.001042 0.001270 0.000789 0.000841 0.000903 0.001259 0.000983
Nuclear Periphery Foci 0.000451 0.001142 0.000944 0.001949 0.000595 0.000399 0.000882 0.001326 0.000511 0.000783 0.000911 0.001643
Nucleolus 0.001820 0.002461 0.000365 0.000239 0.001598 0.001934 0.000380 0.000584 0.001727 0.002206 0.000373 0.000408
Nucleus 0.463548* 0.443383* 0.699324* 0.228692* 0.550237* 0.585848* 0.641458* 0.174023* 0.499919* 0.512270* 0.668004* 0.201824*
Peroxisomes 0.000858 0.001979 0.000119 0.000524 0.000879 0.001853 0.000055 0.001845 0.000867 0.001918 0.000084 0.001173
Vacuole 0.013457 0.037890 0.017872 0.040595 0.017437 0.022106 0.025799 0.086772 0.015127 0.030258 0.022162 0.063290
Vacuole Periphery 0.000556 0.001146 0.000107 0.001985 0.000256 0.000491 0.000132 0.002118 0.000430 0.000830 0.000121 0.002050

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.06 -7.82 -12.66 -3.79 -0.34 -2.98 -15.41 -9.00 -2.67 -2.16 -7.10 -12.39 -15.41 -4.44 -1.65
Bud Neck -4.61 -0.17 -1.05 4.05 -1.03 -5.43 -4.98 -6.91 2.30 -0.96 -6.81 -2.90 -4.34 4.48 -1.36
Bud Site -6.55 -0.90 -7.75 -1.98 -6.76 -4.89 -3.93 -8.63 -3.89 -6.23 -7.97 -2.79 -11.33 -4.04 -9.20
Cell Periphery 0.08 6.12 1.09 0.96 -2.83 -0.66 -1.62 0.88 1.14 2.44 0.05 4.28 1.76 1.47 -1.41
Cytoplasm 2.34 21.77 -4.64 -6.22 -24.05 4.07 9.42 -9.81 -13.87 -20.77 5.13 22.31 -9.82 -13.97 -31.49
Cytoplasmic Foci 0.31 21.12 15.17 11.59 -10.31 2.96 13.95 9.14 6.01 -11.15 2.50 25.24 17.57 12.81 -15.21
Eisosomes 5.07 12.78 13.07 8.25 -0.42 0.91 3.84 4.23 2.22 0.63 4.89 12.88 13.29 7.07 0.28
Endoplasmic Reticulum -7.00 -11.11 -4.09 3.38 8.51 -2.43 -12.00 -2.56 1.05 8.51 -6.32 -15.32 -4.77 2.84 11.87
Endosome -3.47 5.31 4.10 6.68 -1.45 -1.67 5.54 0.92 2.32 -3.94 -3.68 7.27 3.64 6.65 -4.03
Golgi -2.00 3.57 -0.59 0.76 -1.72 -1.26 2.17 -0.87 0.75 -2.20 -2.14 4.17 -0.89 1.07 -2.54
Lipid Particles 1.87 5.05 3.31 2.89 -7.35 0.51 5.74 3.17 2.12 -7.89 1.95 6.68 4.25 3.51 -10.51
Mitochondria -1.43 1.77 0.08 1.63 -1.89 -3.21 -5.96 -2.67 1.27 -1.90 -3.18 1.13 -1.37 1.91 -2.67
Mitotic Spindle -1.78 -3.14 -9.49 -8.67 -7.17 -2.48 -1.45 -9.53 -7.13 -9.08 -3.17 -3.47 -13.56 -10.99 -11.57
None 2.67 8.14 5.61 3.62 -6.98 0.87 0.89 0.69 -0.37 -0.37 3.02 6.82 5.16 2.51 -3.58
Nuclear Periphery -2.13 -10.68 -8.47 -6.95 -1.02 0.18 -1.02 -0.62 -2.24 2.38 -0.46 -3.77 -3.49 -6.06 0.53
Nuclear Periphery Foci -3.05 -4.82 -5.49 -2.08 -2.13 1.40 -3.76 -4.56 -5.58 -0.58 -2.19 -6.18 -7.04 -4.09 -2.17
Nucleolus -1.67 7.06 6.09 7.02 -1.33 -1.07 3.26 1.90 3.41 -1.66 -1.85 6.93 4.94 6.97 -1.99
Nucleus 1.74 -22.73 13.91 10.33 35.00 -2.15 -8.16 24.29 25.77 36.61 -1.01 -21.89 26.76 24.99 49.99
Peroxisomes -3.41 4.29 2.95 6.34 -2.22 -1.59 1.99 0.69 2.83 -1.87 -3.22 3.88 1.82 5.64 -2.49
Vacuole -10.72 -6.85 -23.92 -15.52 -18.50 -2.87 -9.51 -26.42 -24.47 -19.09 -10.16 -12.16 -35.48 -28.42 -25.76
Vacuole Periphery -2.89 4.15 -0.07 1.96 -2.05 -2.39 2.29 -2.36 -0.93 -3.43 -3.36 4.69 -1.15 1.25 -3.59
Standard name
Human Ortholog
Description DNA replication licensing factor; required for pre-replication complex assembly
Localization
Cell Percentages cytoplasm (37%), nucleus (18%), mixed (43%)
Cell Cycle Regulation Yes
cytoplasm - S/G2 (2.7e-09)
cytoplasm - MA (3.3e-08)
nucleus - T/G1 (6.8e-15)
Subcompartmental Group N/A

Tah11

Tah11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tah11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available