Standard name
Human Ortholog
Description Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication; human EIF5A complements the inviability of the yeast hyp2 anb1 double null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.93 0.92 0.92 0.83 – 0.74 0.63 0.69 0.59 0.61 0.98 0.98 0.98 0.97 0.99 0.95 0.89 0.86 0.72 0.76 0.82 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.07 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0.08 0.33 0.15 0.42 – 0.45 0.69 0.56 0.63 0.61 0 0 0 0 0 0 0 0.06 0.09 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 7 1 3 0 1 3
Bud 1 0 0 0 1 0 – 8 7 9 7 9 0 1 0 0 0 0 13 0 0 6 6 7
Bud Neck 2 0 1 0 1 0 – 4 0 2 1 1 0 0 0 0 0 1 2 0 1 2 0 2
Bud Site 0 0 1 0 0 0 – 2 0 0 2 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 11 4 0 2 1 1 – 1 0 5 5 2 1 1 1 7 7 2 3 0 0 0 0 0
Cytoplasm 713 119 136 86 181 198 – 235 186 221 132 176 178 256 264 161 175 101 635 109 98 93 164 180
Endoplasmic Reticulum 1 0 3 1 1 0 – 1 0 0 0 0 2 2 0 9 12 10 0 0 1 0 1 1
Endosome 0 0 0 0 0 2 – 1 0 1 0 0 0 1 0 0 0 1 6 1 3 0 3 3
Golgi 0 0 0 0 1 0 – 0 0 0 0 0 0 1 0 1 1 0 2 0 0 2 3 0
Mitochondria 18 11 12 31 30 102 – 143 201 179 141 176 1 2 1 4 3 3 14 7 12 5 5 4
Nucleus 2 0 0 0 0 2 – 4 0 0 0 1 0 0 1 1 1 0 0 1 0 0 1 0
Nuclear Periphery 0 0 0 0 1 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 2 0 – 0 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 1 – 8 0 3 4 9 0 2 0 0 0 0 9 0 2 3 3 1
Vac/Vac Membrane 3 0 0 0 2 0 – 6 1 1 1 2 1 0 2 0 1 2 5 1 3 3 3 3
Unique Cell Count 729 122 147 93 197 240 318 293 318 225 287 182 262 269 166 176 106 716 128 136 123 201 214
Labelled Cell Count 752 134 154 120 221 307 413 395 422 293 382 183 266 270 183 200 121 716 128 136 123 201 214


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.1 3.1 3.5 4.0 3.1 – 3.2 2.7 2.9 2.9 2.7 5.2 5.1 5.0 6.0 6.2 5.8 4.7 5.2 6.0
Std Deviation (1e-4) 0.7 0.8 0.8 2.1 2.0 1.2 – 1.5 1.4 1.2 1.3 1.1 1.7 1.9 1.8 1.3 1.6 1.3 1.7 2.0 2.3
Intensity Change (Log2) – – – 0.2 0.37 0.01 – 0.04 -0.17 -0.1 -0.09 -0.21 0.75 0.74 0.71 0.97 1.0 0.92 0.62 0.75 0.96


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.3394 -0.8815 -0.9586 -0.8682 -0.9813 -0.7357 0.0838 -0.5055 -0.4905 -1.027 1.5789 -0.6825 -0.2498 0.2073 0.1573 0.4398 0.2265 -0.1975
Actin 0.0117 0.0002 0.0173 0.0317 0.1604 0.0055 0.0864 0.013 0.0237 0.0168 0.0303 0.0206 0.0093 0.0006 0.0059 0.0585 0.0068 0.0009
Bud 0 0.0003 0.0004 0.0003 0.0007 0 0.0082 0.0166 0.005 0.012 0.0135 0.0031 0.0028 0.0005 0.0002 0.006 0.0001 0.0001
Bud Neck 0.0002 0 0.0064 0.0022 0.0037 0.0002 0.0033 0.0004 0.0205 0.0002 0.0008 0.0014 0.0165 0.0001 0.0004 0.0057 0.0016 0.0004
Bud Periphery 0.0001 0.0003 0.0008 0.0003 0.0011 0 0.0065 0.0083 0.0027 0.0147 0.0165 0.0015 0.004 0.0004 0.0002 0.0143 0.0001 0.0002
Bud Site 0.0002 0.0003 0.0005 0.002 0.0068 0 0.0123 0.0288 0.0194 0.0004 0.004 0.001 0.0127 0.0101 0.0021 0.0199 0.0008 0.0001
Cell Periphery 0.0001 0 0.0001 0.0001 0.0003 0 0.0006 0.0008 0.0011 0.0004 0.0005 0.0001 0.0006 0.0001 0.0001 0.0004 0.0001 0
Cytoplasm 0.032 0.0243 0.0609 0.1065 0.1074 0.0944 0.0855 0.1129 0.1205 0.0884 0.0899 0.1347 0.06 0.1154 0.0835 0.1656 0.0477 0.1479
Cytoplasmic Foci 0.0165 0.0039 0.0052 0.0266 0.0384 0.0045 0.0661 0.0145 0.0097 0.015 0.0521 0.0205 0.0083 0.0024 0.0161 0.0128 0.0048 0.0107
Eisosomes 0.0005 0.0001 0.0005 0.0005 0.0018 0.0002 0.0008 0.0003 0.0006 0.0005 0.0003 0.0002 0.0004 0.0001 0.0003 0.0008 0.0007 0.0001
Endoplasmic Reticulum 0.0006 0.0003 0.001 0.0023 0.001 0.0004 0.0033 0.0016 0.0027 0.0007 0.0157 0.0013 0.0024 0.0006 0.0014 0.0022 0.0021 0.0007
Endosome 0.0021 0.0003 0.0035 0.0216 0.0183 0.0053 0.0534 0.0009 0.0083 0.0224 0.0366 0.0097 0.0037 0.0003 0.0076 0.0351 0.0173 0.003
Golgi 0.0012 0.0001 0.0016 0.007 0.0059 0.0014 0.0149 0.0002 0.01 0.0531 0.0666 0.0227 0.0009 0.0001 0.0015 0.0032 0.0012 0.0003
Lipid Particles 0.0056 0.0004 0.003 0.0226 0.0103 0.004 0.0628 0.0003 0.0047 0.0179 0.0306 0.0283 0.0029 0.0001 0.0036 0.0043 0.0033 0.0007
Mitochondria 0.0029 0.0003 0.0011 0.0039 0.0025 0.0004 0.0058 0.0014 0.0186 0.015 0.1546 0.0242 0.0016 0.0001 0.0005 0.0014 0.001 0.0002
None 0.9115 0.9675 0.8918 0.7308 0.6216 0.8749 0.5126 0.792 0.7248 0.7299 0.2781 0.6678 0.8448 0.8679 0.8633 0.6496 0.8527 0.8186
Nuclear Periphery 0.0003 0.0001 0.0007 0.0095 0.0017 0.0042 0.0081 0.0006 0.0064 0.0005 0.0317 0.0007 0.0021 0.0001 0.0015 0.0055 0.0119 0.0003
Nucleolus 0.0002 0.0002 0.0003 0.001 0.0014 0.0001 0.0009 0.0009 0.0018 0.0002 0.0003 0.0004 0.0069 0.0001 0.0006 0.001 0.0057 0.0004
Nucleus 0.0003 0.0002 0.0004 0.0016 0.0018 0.0003 0.0028 0.0024 0.0061 0.0008 0.0162 0.0034 0.0052 0.0002 0.0009 0.0017 0.0113 0.0004
Peroxisomes 0.0129 0.0003 0.0026 0.0186 0.0049 0.0014 0.0462 0.0009 0.0086 0.0082 0.1465 0.0444 0.0013 0.0001 0.002 0.0023 0.0008 0.0008
Punctate Nuclear 0.0006 0.0006 0.0009 0.0091 0.0076 0.0024 0.0078 0.002 0.0021 0.0006 0.0012 0.0129 0.0115 0.0003 0.0076 0.006 0.0273 0.0139
Vacuole 0.0004 0.0002 0.0009 0.0009 0.0018 0.0001 0.0086 0.001 0.002 0.0013 0.0093 0.0007 0.0019 0.0003 0.0006 0.0026 0.0019 0.0003
Vacuole Periphery 0.0001 0 0.0002 0.0009 0.0005 0.0002 0.003 0.0001 0.0007 0.0008 0.0047 0.0005 0.0004 0 0.0001 0.0009 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 288.6543 355.8446 376.776 207.4643 259.5625 335.0259 233.0245 207.3343 214.9711 127.9684
Translational Efficiency 2.1537 2.226 1.6831 3.1302 2.2443 2.005 2.3153 2.9642 2.2732 2.6852

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication; human EIF5A complements the inviability of the yeast hyp2 anb1 double null mutant
Localization
Cell Percentages cytoplasm (75%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Anb1

Anb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Anb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available